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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMXs-IRES-GFP_IgD (#RDB14502)

Retroviral expression vector of mouse Immunoglobulin D (IgD).

Clone info. Retroviral expression vector of mouse Immunoglobulin D (IgD), cloned into the pMXs-IRES-GFP. Refer Fig.1 of Haniuda, K., Nat. Immunol., 17 (9): 1109-1117 (2016).
Comment Expression was confirmed by the depositor with flow cytometry. Sequence primer for IgH 5term-GCGGTCTATTATTGTGCAAG-3term.
Vector backbone pMXs-IRES-GFP (Plasmid)
Selectable markers Amp^r (E. coli)
Gene/insert name mouse Ighd cDNA
Depositor|Developer Kitamura, Daisuke |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested.
Additional terms and conditions:
Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 （Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016） を引用し、寄託者への謝辞の表明を必要とする。
MTAに書く付加的使用条件：
pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14502 pMXs-IRES-GFP_IgD DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-IRES-GFP_IgD was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14502).

Reference section:

Haniuda, K., Fukao, S., Kodama, T., Hasegawa, H., Kitamura, D., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14502_A6Lep1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5'
Sequence file: RDB14502_A6Lea.seq check
>D04073A2_A6Le_1_pBABE5'_A01_01_ABI24.ab1
    1 GCTTTAATAT GGGCATATGA GATCTTATAT GGGGCACCCC CGCCCCTTGT AAACTTCCCT
   61 GACCCTGACA TGACAAGAGT TACTAACAGC CCCTCTCTCC AAGCTCACTT ACAGGCTCTC
  121 TACTTAGTCC AGCACGAAGT CTGGAGACCT CTGGCGGCAG CCTACCAAGA ACAACTGGAC
  181 CGACCGGTGG TACCTCACCC TTACCGAGTC GGCGACACAG TGTGGGTCCG CCGACACCAG
  241 ACTAAGAACC TAGAACCTCG CTGGAAAGGA CCTTACACAG TCCTGCTGAC CACCCCCACC
  301 GCCCTCAAAG TAGACGGCAT CGCAGCTTGG ATACACGCCG CCCACGTGAA GGCTGCCGAC
  361 CCCGGGGGTG GACCATCCTC TAGACTGCCG GATCCCAGTG TGGTGGTACG GGAATTCACA
  421 CAGGACCTCA CCATGGGATG GAGCTGTATC ATGCTCTTCT TGGCAGCAAC AGCTACAGGT
  481 GTCCACTCCC AGGTCCAACT GCAGCAGCCT GGGGCTGAGC TTGTGAAGCC TGGGGCTTCA
  541 GTGAAGCTGT CCTGCAAGGC TTCTGGCTAC ACCTTCACCA GCTACTTGAT GCACTGGGTG
  601 AAGCAGAGGC CTGGACGAGG CCTTGAGTGG ATTGGAAGGA TTGATCCTAA TAGTGGTGGT
  661 ACTAAGTACA ATGAGAAGTT CAAGAGCAAG GCCACACTGA CTGTAGACAA ACCCTCCAGC
  721 ACAGCCTACA TGCAGCTCAG CAGCCTGACA TCTGAGGACT CTGCGGTCTA TTATTGTGCA
  781 AGATATGATT ACTACGGTAG TAGCTACTTT GACTACTGGG GCCAAGGCAC CACTCTCACA
  841 GTCTCGAGTG GTAATGAAAA GGGACCTGAC ATGTTCCTCC TCTCAGAGTG CAAAGCCCCA
  901 GAGGAAAATG AAAAGATAAA CCTGGGCTGT TTAGTAATTG GAAGTCAGCC ACTGAAAATC
  961 AGCTGGGAGC CAAAGAAGTC AAGTATAGTT GAACATGTCT TCCCCTCTGA AATGAGAAAT
 1021 GGCAATTATA CAATGGTCCT CCAGGGTCAC TGTGCTGGGC TCAGAACTGA ACCTCAACCA
 1081 CACTTGCACA TTAATTAAAC CCAAAAGGAA GAAATGTTCC AGCTTCTGAG TCATGGGATT
1141 CCCAGTCCCT CTTAAGGAAG AGAGAG
//
Primer: IRES-R
Sequence file: RDB14502_A6Leb.seq check
>D04073A2_A6Le_1_IRES-R_B02_05_ABI24.ab1
    1 GCATCAGCGG CTTCGGCAGT ACGTTAGGGG GGGGGGAGGG AGAGGGGGGG GGGGGGCGGA
   61 ATTTACGTAG CGGCCGCCTA CTTTACCTTG ATGAAGGTGA CGAAGCCACT GTAGAGCAGT
  121 GTGAGCAGGA AGAGGGCCAC GAAGGTGCAC ATTGTGGGCC ACAGGCCGTT CTCCTCCTCT
  181 AAGTCCATGT AGCTGTCACT TTGCTCATCC ATGATACACG AGTGTTGGAT GGTGTTGACT
  241 ATGCCACTAA TTGCTAGGCT CTTGCTGGCA TTAAGCTTTG TCTTTGAGGC CTCATGTTCC
  301 ACCACACATG TGTAAGTGTC AAGTGATGAG CTCAAAGCGA CTGGTAGTCT CAGGACACTC
  361 CAGGTCTGGA ATGTGCCCCC AGGCTGGGGG GTGGGGTTTG CAGTGACAAA GTTTGTAGAC
  421 TTCATTTTAC TGTGGACACT CAGCCACATG AGGTGGATAT TTTCGGGGAA GAAGCCAGAT
  481 ACTTCACACA GGAGCCAAGA TGACATCTCA GGATGGGTGG ATGTGGTCAG GATGTTCACA
  541 GTGAGGTTGC TGGGTGCCAT GGCCCCTTCT ATGTCCTTTT TGGTGGTAAT AGCTTTGGTG
  601 GCTTCTGTGG AGTGATTCTT TGCTTGGAGA GTTGGAGTGA CTCTCTTAGA GGACTGGGAA
  661 TCCCATGACT CAGGAAACTT GAAAGGTTTT TCTTTCCTTT TGGGTTTATT TATGGTGCAA
  721 GTGTGGTTGA GGTTCAGTTC TGAGGCCAGC ACAGTGACCT GGAGGACCAT TGTATAATTG
  781 CCATTTCTCA TTTCAGAGGG GAAGACATGT TCAACTATAC TTGACTTCTT TGGCTCCCAG
  841 CTGATTTTCA GTGGCTGACT TCCAATTACT AAACAGCCCA GGTTTATCTT TTCATTTTCC
  901 TCTGGGGCTT TGCACTCTGA GAGGAGGAAC ATGTCAGGTC CCTTTTCATT ACCACTCGAG
  961 ACTGTGAGAG TGGTGCCTTT GGCCCCAGTA GTCAAAGTAG CTACTACCGT AGTAATCATA
 1021 TCTTGCACAA TAATAGACCC GCAGAGTCCT CAGATGTCAG GCTGCTGAGC TGCATGTAGC
 1081 TGTGCTTGGA GGATTTGTCT ACAGGTCAGT GTGGCTGCTC ATGAACTCTC AATTGTACCT
1141 TAGTACATCA CTAATAGATC GACTTCCGAT
//
Primer: IRES-F
Sequence file: RDB14502_A6Lec.seq check
>D04073A2_A6Le_1_IRES-F_C05_08_ABI24.ab1
    1 GGCATCTTCT GTGTTTAGTC GAGGTTAAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG
   61 TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGGTGAG CAAGGGCGAG
  121 GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC
  181 AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG
  241 TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC
  301 TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG
  361 TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC
  421 TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG
  481 AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC
  541 AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC
  601 AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC
  661 ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC
  721 GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC
  781 GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT AAAGCGGCCG GCCGCCAGCA
  841 CAGTGGTCGA CGATAAAATA AAAGATTTTA TTTAGTCTCC AGAAAAAGGG GGGAATGAAA
  901 GACCCCACCT GTAGGTTTGG CAAGCTAGCT TAAGTAACGC CATTTTGCAA GGCATGGAAA
  961 AATACATAAA CTGAGAATAG AGAAAGTTCA GATCAAGGTC AGGAACAGAT GGGAACAGCT
 1021 GATATGGGCC CAAACAGGGA TATCTGTGGT AAGCAGTTTC CCTGCCCCCG GCTTCAGATC
1081 AGAACAGATG GACAGCTGAA TTATGGGTCC C
//
Primer: Amp_R
Sequence file: RDB14502_A6Led.seq check
>D04073A2_A6Le_1_Amp_R_D05_11_ABI24.ab1
    1 CTTCGTTTCA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGCATAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT
  181 GCAAGGCATG GAAAAATACA TAACTGAGAA TAGAAAAGTT CAGATCAAGG TCAGGAACAG
  241 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT
  301 CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA
  361 GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC
  421 AGTTTCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG
  481 CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC
  541 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGCGCGC CAGTCCTCCG ATTGACTGAG
  601 TCGCCCGGGT ACCCGTGTAT CCAATAAACC CTCTTGCAGT TGCATCCGAC TTGTGGTCTC
  661 GCTGTTCCTT GGGAGGGTCT CCTCTGAGTG ATTGACTACC CGTCAGCGGG GGTCTTTCAT
  721 TTGGGGGCTC GTCCGGGATC GGGAGACCCC TGCCCAGGGA CCACCGACCC ACCACCGGGA
  781 GGTAAGCTGG CCAGCAACTT ATCTGTGTCT GTCCGATTGT CTAGTGTCTA TGACTGATTT
  841 TATGCGCCTG CGTCGGTACT AGTTAGCTAA CTAGCTCTGT ATCTGGCGGA CCCGTGGTGG
  901 AACTGACGAG TTCGGAACAC CCGGCCGCAA CCCTGGGAGA CGTCCCAGGG ACTTCTGGGG
  961 CCGTTTTTTG TGGCCCGACC TGAGTCCCAA AAATCCCGAT CGTTTTGGGA CTCTTTGGGT
 1021 GCACCCCCCT TAGAGGAAGG ATATGTGGAT TCCTGTAGAG ACGAGGAACC CTAACAGTCC
1081 GGCCATCGGT CTGAGTTTTG CTTTCCGGAT TTTGGGGGGG A
//
Primer: SV40pA3'element Reverse
Sequence file: RDB14502_A6Lee.seq check
>D04073A2_A6Le_1_SV40pA3element Reverse_E05_14_ABI24.ab1
    1 GAGGTTAGGG GGGCGGACCT ATGGAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC
   61 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA
  121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG
  181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG
  241 TTGGCCGATT CATTAATGCA ACTATGGCCA TTTAATGTAA ATACTTAAGA AAAAAAACCA
  301 AATTAATTTT GATACATGCT GCATGTGAAA GACCCCCGCT GACGGGTAGT CAATCACTCA
  361 GAGGAGACCC TCCCAAGGAA CAGCGAGACC ACAAGTCGGA TGCAACTGCA AGAGGGTTTA
  421 TTGGATACAC GGGTACCCGG GCGACTCAGT CAATCGGAGG ACTGGCGCCC CGAGTGAGGG
  481 GTTGTGGGCT CTTTTATTGA GCTCGGGGAG CAGAAGCGCG CGAACAGAAG CGAGAAGCGA
  541 ACTGATTGGT TAGTTCAAAT AAGGCACAGG GTCATTTCAG GTCCTTGGGG CACCCTGGAA
  601 ACATCTGATG GTTCTCTAGA AACTGCTGAG GGCTGGACCG CATCTGGGGA CCATCTGTTC
  661 TTGGCCCTGA GCCGGGGCAG GAACTGCTTA CCACAGATAT CCTGTTTGGC CCATATTCAG
  721 CTGTTCCATC TGTTCTTGGC CCTGAGCCGG GGCAGGAACT GCTTACCACA GATATCCTGT
  781 TTGGCCCATA TTCAGCTGTT CCATCTGTTC CTGACCTTGA TCTGAACTTC TCTATTCTCA
  841 GTTATGTATT TTTTCCATGC CTTGCAAAAT GGCGTTACTT AAGCTAGCTT GCCAAACCTA
  901 CAGGTGGGGG TCTTTCATTC CCCCCCTTTT TTCTGGAGAC TAAATAAAAA TCTTTTTATT
  961 TTTATCGTCG ACCCACTGTG CTGGCGGGCC GGCCCGCTTT TACTTTGTAC AGCTCGTCCA
 1021 TGCCGAGAGT TGATCCCCGG CGGGCGGGTC ACGACCTCCA GCACGACCAT GTGAATCGCG
1081 CATCTCGTTA GGATCTTTTG CTCCAAGGGC GGGCA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827. [link to RRC of NBRP]