Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMXs-IRES-GFP_IgD

Retroviral expression vector of mouse Immunoglobulin D (IgD).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested. Additional terms and conditions: Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 （Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016） を引用し、寄託者への謝辞の表明を必要とする。 付加的提供条件： pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14502_A6Lep1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5' Sequence file: RDB14502_A6Lea.seq
>D04073A2_A6Le_1_pBABE5'_A01_01_ABI24.ab1     1 GCTTTAATAT GGGCATATGA GATCTTATAT GGGGCACCCC CGCCCCTTGT AAACTTCCCT    61 GACCCTGACA TGACAAGAGT TACTAACAGC CCCTCTCTCC AAGCTCACTT ACAGGCTCTC   121 TACTTAGTCC AGCACGAAGT CTGGAGACCT CTGGCGGCAG CCTACCAAGA ACAACTGGAC   181 CGACCGGTGG TACCTCACCC TTACCGAGTC GGCGACACAG TGTGGGTCCG CCGACACCAG   241 ACTAAGAACC TAGAACCTCG CTGGAAAGGA CCTTACACAG TCCTGCTGAC CACCCCCACC   301 GCCCTCAAAG TAGACGGCAT CGCAGCTTGG ATACACGCCG CCCACGTGAA GGCTGCCGAC   361 CCCGGGGGTG GACCATCCTC TAGACTGCCG GATCCCAGTG TGGTGGTACG GGAATTCACA   421 CAGGACCTCA CCATGGGATG GAGCTGTATC ATGCTCTTCT TGGCAGCAAC AGCTACAGGT   481 GTCCACTCCC AGGTCCAACT GCAGCAGCCT GGGGCTGAGC TTGTGAAGCC TGGGGCTTCA   541 GTGAAGCTGT CCTGCAAGGC TTCTGGCTAC ACCTTCACCA GCTACTTGAT GCACTGGGTG   601 AAGCAGAGGC CTGGACGAGG CCTTGAGTGG ATTGGAAGGA TTGATCCTAA TAGTGGTGGT   661 ACTAAGTACA ATGAGAAGTT CAAGAGCAAG GCCACACTGA CTGTAGACAA ACCCTCCAGC   721 ACAGCCTACA TGCAGCTCAG CAGCCTGACA TCTGAGGACT CTGCGGTCTA TTATTGTGCA   781 AGATATGATT ACTACGGTAG TAGCTACTTT GACTACTGGG GCCAAGGCAC CACTCTCACA   841 GTCTCGAGTG GTAATGAAAA GGGACCTGAC ATGTTCCTCC TCTCAGAGTG CAAAGCCCCA   901 GAGGAAAATG AAAAGATAAA CCTGGGCTGT TTAGTAATTG GAAGTCAGCC ACTGAAAATC   961 AGCTGGGAGC CAAAGAAGTC AAGTATAGTT GAACATGTCT TCCCCTCTGA AATGAGAAAT  1021 GGCAATTATA CAATGGTCCT CCAGGGTCAC TGTGCTGGGC TCAGAACTGA ACCTCAACCA  1081 CACTTGCACA TTAATTAAAC CCAAAAGGAA GAAATGTTCC AGCTTCTGAG TCATGGGATT 1141 CCCAGTCCCT CTTAAGGAAG AGAGAG //
Primer: IRES-R Sequence file: RDB14502_A6Leb.seq
>D04073A2_A6Le_1_IRES-R_B02_05_ABI24.ab1     1 GCATCAGCGG CTTCGGCAGT ACGTTAGGGG GGGGGGAGGG AGAGGGGGGG GGGGGGCGGA    61 ATTTACGTAG CGGCCGCCTA CTTTACCTTG ATGAAGGTGA CGAAGCCACT GTAGAGCAGT   121 GTGAGCAGGA AGAGGGCCAC GAAGGTGCAC ATTGTGGGCC ACAGGCCGTT CTCCTCCTCT   181 AAGTCCATGT AGCTGTCACT TTGCTCATCC ATGATACACG AGTGTTGGAT GGTGTTGACT   241 ATGCCACTAA TTGCTAGGCT CTTGCTGGCA TTAAGCTTTG TCTTTGAGGC CTCATGTTCC   301 ACCACACATG TGTAAGTGTC AAGTGATGAG CTCAAAGCGA CTGGTAGTCT CAGGACACTC   361 CAGGTCTGGA ATGTGCCCCC AGGCTGGGGG GTGGGGTTTG CAGTGACAAA GTTTGTAGAC   421 TTCATTTTAC TGTGGACACT CAGCCACATG AGGTGGATAT TTTCGGGGAA GAAGCCAGAT   481 ACTTCACACA GGAGCCAAGA TGACATCTCA GGATGGGTGG ATGTGGTCAG GATGTTCACA   541 GTGAGGTTGC TGGGTGCCAT GGCCCCTTCT ATGTCCTTTT TGGTGGTAAT AGCTTTGGTG   601 GCTTCTGTGG AGTGATTCTT TGCTTGGAGA GTTGGAGTGA CTCTCTTAGA GGACTGGGAA   661 TCCCATGACT CAGGAAACTT GAAAGGTTTT TCTTTCCTTT TGGGTTTATT TATGGTGCAA   721 GTGTGGTTGA GGTTCAGTTC TGAGGCCAGC ACAGTGACCT GGAGGACCAT TGTATAATTG   781 CCATTTCTCA TTTCAGAGGG GAAGACATGT TCAACTATAC TTGACTTCTT TGGCTCCCAG   841 CTGATTTTCA GTGGCTGACT TCCAATTACT AAACAGCCCA GGTTTATCTT TTCATTTTCC   901 TCTGGGGCTT TGCACTCTGA GAGGAGGAAC ATGTCAGGTC CCTTTTCATT ACCACTCGAG   961 ACTGTGAGAG TGGTGCCTTT GGCCCCAGTA GTCAAAGTAG CTACTACCGT AGTAATCATA  1021 TCTTGCACAA TAATAGACCC GCAGAGTCCT CAGATGTCAG GCTGCTGAGC TGCATGTAGC  1081 TGTGCTTGGA GGATTTGTCT ACAGGTCAGT GTGGCTGCTC ATGAACTCTC AATTGTACCT 1141 TAGTACATCA CTAATAGATC GACTTCCGAT //
Primer: IRES-F Sequence file: RDB14502_A6Lec.seq
>D04073A2_A6Le_1_IRES-F_C05_08_ABI24.ab1     1 GGCATCTTCT GTGTTTAGTC GAGGTTAAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG    61 TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGGTGAG CAAGGGCGAG   121 GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC   181 AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG   241 TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC   301 TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG   361 TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC   421 TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG   481 AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC   541 AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC   601 AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC   661 ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC   721 GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC   781 GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT AAAGCGGCCG GCCGCCAGCA   841 CAGTGGTCGA CGATAAAATA AAAGATTTTA TTTAGTCTCC AGAAAAAGGG GGGAATGAAA   901 GACCCCACCT GTAGGTTTGG CAAGCTAGCT TAAGTAACGC CATTTTGCAA GGCATGGAAA   961 AATACATAAA CTGAGAATAG AGAAAGTTCA GATCAAGGTC AGGAACAGAT GGGAACAGCT  1021 GATATGGGCC CAAACAGGGA TATCTGTGGT AAGCAGTTTC CCTGCCCCCG GCTTCAGATC 1081 AGAACAGATG GACAGCTGAA TTATGGGTCC C //
Primer: Amp_R Sequence file: RDB14502_A6Led.seq
>D04073A2_A6Le_1_Amp_R_D05_11_ABI24.ab1     1 CTTCGTTTCA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT    61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA   121 CCTGCATAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT   181 GCAAGGCATG GAAAAATACA TAACTGAGAA TAGAAAAGTT CAGATCAAGG TCAGGAACAG   241 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT   301 CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA   361 GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC   421 AGTTTCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG   481 CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC   541 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGCGCGC CAGTCCTCCG ATTGACTGAG   601 TCGCCCGGGT ACCCGTGTAT CCAATAAACC CTCTTGCAGT TGCATCCGAC TTGTGGTCTC   661 GCTGTTCCTT GGGAGGGTCT CCTCTGAGTG ATTGACTACC CGTCAGCGGG GGTCTTTCAT   721 TTGGGGGCTC GTCCGGGATC GGGAGACCCC TGCCCAGGGA CCACCGACCC ACCACCGGGA   781 GGTAAGCTGG CCAGCAACTT ATCTGTGTCT GTCCGATTGT CTAGTGTCTA TGACTGATTT   841 TATGCGCCTG CGTCGGTACT AGTTAGCTAA CTAGCTCTGT ATCTGGCGGA CCCGTGGTGG   901 AACTGACGAG TTCGGAACAC CCGGCCGCAA CCCTGGGAGA CGTCCCAGGG ACTTCTGGGG   961 CCGTTTTTTG TGGCCCGACC TGAGTCCCAA AAATCCCGAT CGTTTTGGGA CTCTTTGGGT  1021 GCACCCCCCT TAGAGGAAGG ATATGTGGAT TCCTGTAGAG ACGAGGAACC CTAACAGTCC 1081 GGCCATCGGT CTGAGTTTTG CTTTCCGGAT TTTGGGGGGG A //
Primer: SV40pA3'element Reverse Sequence file: RDB14502_A6Lee.seq
>D04073A2_A6Le_1_SV40pA3element Reverse_E05_14_ABI24.ab1     1 GAGGTTAGGG GGGCGGACCT ATGGAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC    61 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA   121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG   181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG   241 TTGGCCGATT CATTAATGCA ACTATGGCCA TTTAATGTAA ATACTTAAGA AAAAAAACCA   301 AATTAATTTT GATACATGCT GCATGTGAAA GACCCCCGCT GACGGGTAGT CAATCACTCA   361 GAGGAGACCC TCCCAAGGAA CAGCGAGACC ACAAGTCGGA TGCAACTGCA AGAGGGTTTA   421 TTGGATACAC GGGTACCCGG GCGACTCAGT CAATCGGAGG ACTGGCGCCC CGAGTGAGGG   481 GTTGTGGGCT CTTTTATTGA GCTCGGGGAG CAGAAGCGCG CGAACAGAAG CGAGAAGCGA   541 ACTGATTGGT TAGTTCAAAT AAGGCACAGG GTCATTTCAG GTCCTTGGGG CACCCTGGAA   601 ACATCTGATG GTTCTCTAGA AACTGCTGAG GGCTGGACCG CATCTGGGGA CCATCTGTTC   661 TTGGCCCTGA GCCGGGGCAG GAACTGCTTA CCACAGATAT CCTGTTTGGC CCATATTCAG   721 CTGTTCCATC TGTTCTTGGC CCTGAGCCGG GGCAGGAACT GCTTACCACA GATATCCTGT   781 TTGGCCCATA TTCAGCTGTT CCATCTGTTC CTGACCTTGA TCTGAACTTC TCTATTCTCA   841 GTTATGTATT TTTTCCATGC CTTGCAAAAT GGCGTTACTT AAGCTAGCTT GCCAAACCTA   901 CAGGTGGGGG TCTTTCATTC CCCCCCTTTT TTCTGGAGAC TAAATAAAAA TCTTTTTATT   961 TTTATCGTCG ACCCACTGTG CTGGCGGGCC GGCCCGCTTT TACTTTGTAC AGCTCGTCCA  1021 TGCCGAGAGT TGATCCCCGG CGGGCGGGTC ACGACCTCCA GCACGACCAT GTGAATCGCG 1081 CATCTCGTTA GGATCTTTTG CTCCAAGGGC GGGCA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl