Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMXs-IRES-GFP_IgM (#RDB14501)

Retroviral expression vector of mouse Immunoglobulin M (IgM).

Clone info. Retroviral expression vector of mouse Immunoglobulin M (IgM), cloned into the pMXs-IRES-GFP. Refer Fig.1 of Haniuda, K., Nat. Immunol., 17 (9): 1109-1117 (2016).
Comment Expression was confirmed by the depositor with flow cytometry. Sequence primer for IgH 5term-GCGGTCTATTATTGTGCAAG-3term.
Vector backbone pMXs-IRES-GFP (Plasmid)
Selectable markers Amp^r (E. coli)
Gene/insert name mouse Ighm cDNA
Depositor|Developer Kitamura, Daisuke |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested.
Additional terms and conditions:
Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 （Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016） を引用し、寄託者への謝辞の表明を必要とする。
MTAに書く付加的使用条件：
pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14501 pMXs-IRES-GFP_IgM DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-IRES-GFP_IgM was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14501).

Reference section:

Haniuda, K., Fukao, S., Kodama, T., Hasegawa, H., Kitamura, D., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14501_A6Lep1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5' (Pr0061)
Region: pol region, insert 5'
Sequence file: RDB14501_A6Lea.seq check
>D04073A1_A6Le_1_pBABE5'_A04_01_ABI24.ab1
    1 GCGATATGGC ATATGAGATC TTATATGGGG CACCCCCGCC CCTTGTAAAC TTCCCTGACC
   61 CTGACATGAC AAGAGTTACT AACAGCCCCT CTCTCCAAGC TCACTTACAG GCTCTCTACT
  121 TAGTCCAGCA CGAAGTCTGG AGACCTCTGG CGGCAGCCTA CCAAGAACAA CTGGACCGAC
  181 CGGTGGTACC TCACCCTTAC CGAGTCGGCG ACACAGTGTG GGTCCGCCGA CACCAGACTA
  241 AGAACCTAGA ACCTCGCTGG AAAGGACCTT ACACAGTCCT GCTGACCACC CCCACCGCCC
  301 TCAAAGTAGA CGGCATCGCA GCTTGGATAC ACGCCGCCCA CGTGAAGGCT GCCGACCCCG
  361 GGGGTGGACC ATCCTCTAGA CTGCCGGATC CCAGTGTGGT GGTACGGGAA TTCACACAGG
  421 ACCTCACCAT GGGATGGAGC TGTATCATGC TCTTCTTGGC AGCAACAGCT ACAGGTGTCC
  481 ACTCCCAGGT CCAACTGCAG CAGCCTGGGG CTGAGCTTGT GAAGCCTGGG GCTTCAGTGA
  541 AGCTGTCCTG CAAGGCTTCT GGCTACACCT TCACCAGCTA CTTGATGCAC TGGGTGAAGC
  601 AGAGGCCTGG ACGAGGCCTT GAGTGGATTG GAAGGATTGA TCCTAATAGT GGTGGTACTA
  661 AGTACAATGA GAAGTTCAAG AGCAAGGCCA CACTGACTGT AGACAAACCC TCCAGCACAG
  721 CCTACATGCA GCTCAGCAGC CTGACATCTG AGGACTCTGC GGTCTATTAT TGTGCAAGAT
  781 ATGATTACTA CGGTAGTAGC TACTTTGACT ACTGGGGCCA AGGCACCACT CTCACAGTCT
  841 CGAGTAGTCA GTCCTTCCCA AATGTCTTCC CCCTCGTCTC CTGCGAGAGC CCCCCTGTCT
  901 GATAAGAATC TGGTGGCCAT GGGCTGCCTG GCCCGGGACT TCCTGCCCAG CACCATTTCC
  961 TTCACCTGGA ACTACCAGAA CAACACTGAA AGTCATCCAG GGGTATCAGA ACCCTTCCCA
 1021 ACACCTGAAG ACAGGGGGCA GTACCTAGCC AACATCGCAA GTGATGGCTG TCTCCTAGAG
1081 CCATCTCAAG ATCAGATGGA GTTACCTGGG GATTGCCGA
//
Primer: IRES-R (Pr0037)
Region: IRES, insert 3'
Sequence file: RDB14501_A6Leb.seq check
>D04073A1_A6Le_1_IRES-R_A02_02_ABI24.ab1
    1 GCATCAGCGG GCTTCGGCCA GTACGTTAGG GGGGGGGGAG GGAGAGGGGG GGGGGGGGCG
   61 GAATTTACGT AGCGGCCGCT CATTTTACCT TGAACAGGGT GACGGTGGTG CTGTAGAAGA
  121 GGCTCAGGAG GAAGAGGACG ATGAAGGTGG AGGCAGTGGT CCACAGGTTC TCAAAGCCTT
  181 CCTCCTCAGC ATTCACCTCC CCCTCAGTGG ACTTGTCCAC GGTCCTCTCG GTCACCAGGT
  241 GTGGCAGGGC CTCGTGGCCT ACAACACAGG TATAGGTCTC TCCGGAGTTC CATTCCTCCT
  301 CTGTCACAGT CAGGATGCTG TGGGTAAAGT AGAAGCCTGG GGCCCCAGGC TCTGGCATCG
  361 GGGCACTGGT CACATACTTC TCTTGGGGCA AGAGTTGCCC TCTCTGAAGC CACTGCACAC
  421 TGATGTCTGC AGGAGAGAAG CCCTTCACCA GGCAGGTGAC TGTGGCTGAC TCCCTCAGGT
  481 TCAGTTGCTC ACGAGCTGGT GGCAGCAGGT ACACAGCAGG TGGATGTTTG TGCACCTCAT
  541 TGGGTTTTGA GATGAATTTC TTCTGTGGTG AAGGCAGATC CCTGTGAGTC ACAGTACACA
  601 CAAATTCCTT CCTGTTATTC CAGTCTTCCA CACAAACACT AGCCACACCC TTAGCACTGA
  661 AGGTGCCATT GGGATGGCTT TCCATGATTT TAATTTTGGT TTCCAGTGGT TCACCACTTT
  721 GAGAAGCCCA GGAGATATTC AGGGTTTCAT AGGTTGCCAG GTTTGAGACC AGACAGGTCA
  781 GGTTAGCGGA CTTGCTGAGG AAGATGTCGG CAAAGGAGGG GGGGATGGTG AAGGTTAGGA
  841 TGTCTGTGGA GGGACTGGCA GCACATGTGG AGGACACGTT CTTCAAGAAG GTGAGACCCC
  901 TGTGATCCAC ACGGCAGTGT ACACATTTCA GGTTCAGCCA GTCGATTTCA GAGATGGTAG
  961 TGTGCTATGA CCCTTGTAGG TTGGGGTGTG ATCCTTTGTT CTCGATGTCA CGAATCTGTG
 1021 TGAGGCAGAT CCACGAGCCT CCCATCATAG CAGATACCTG GATCGGATTG AGTGAAGTTG
1081 ATGCTGCGAT GATTAGACCT GCTGAGTGCA GGCTAGAAGA AG
//
Primer: IRES-F (Pr0036)
Region: IRES, EGFP
Sequence file: RDB14501_A6Lec.seq check
>D04073A1_A6Le_1_IRES-F_C04_07_ABI24.ab1
    1 GGCAAGCCTT CATGTGTTTA GTCGAGGTTA AAAAACGTCT AGGCCCCCCG AACCACGGGG
   61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC
  121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
  181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG
  241 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG
  301 ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
  361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC
  421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG
  481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC
  541 TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC
  601 TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG
  661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG
  721 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG
  781 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAAGCGG CCGGCCGCCA
  841 GCACAGTGGT CGACGATAAA ATAAAAGATT TTATTTAGTC TCCAGAAAAA GGGGGGAATG
  901 AAAGACCCCA CCTGTAGGTT TGGCAAGCTA GCTTAAGTAA CGCCATTTTT GCAAAGGCAT
  961 GGGAAAAAAT ACATAAACTG AGAATAGGAG GAAGTTTCAG GATCAAAGGT CATGAACAGA
 1021 TGGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTTC CTGCCCCGCT
1081 CAGCTAGACA GATGGAACAG CTGAAATAAT GGGGGCCCA
//
Primer: Amp_R (Pr0008)
Region: AmpR pro, 5’LTR, MMLV psi
Sequence file: RDB14501_A6Led.seq check
>D04073A1_A6Le_1_Amp_R_D04_10_ABI24.ab1
    1 CTTCATTTTC ATTATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
   61 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG
  121 CCACCTGCAT AATGAAAGAC CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT
  181 TTTGCAAGGC ATGGAAAAAT ACATAACTGA GAATAGAAAA GTTCAGATCA AGGTCAGGAA
  241 CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG
  301 GCTCAGGGCC AAGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA
  361 GCAGTTCCTG CCCCGGCTCA GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCAGCCCTC
  421 AGCAGTTTCT AGAGAACCAT CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT
  481 GTGCCTTATT TGAACTAACC AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC
  541 CCCGAGCTCA ATAAAAGAGC CCACAACCCC TCACTCGGCG CGCCAGTCCT CCGATTGACT
  601 GAGTCGCCCG GGTACCCGTG TATCCAATAA ACCCTCTTGC AGTTGCATCC GACTTGTGGT
  661 CTCGCTGTTC CTTGGGAGGG TCTCCTCTGA GTGATTGACT ACCCGTCAGC GGGGGTCTTT
  721 CATTTGGGGG CTCGTCCGGG ATCGGGAGAC CCCTGCCCAG GGACCACCGA CCCACCACCG
  781 GGAGGTAAGC TGGCCAGCAA CTTATCTGTG TCTGTCCGAT TGTCTAGTGT CTATGACTGA
  841 TTTTATGCGC CTGCGTCGGT ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG
  901 TGGAACTGAC GAGTTCGGAA CACCCGGGCC GCAACCCTGG GAGACGTCCC AGGGACTTCG
  961 GGGGGCCCGT TTTTTGTGGC CCGACCTGAG TCCAAAAAAT CCCGATCGTT TTGGGACTCT
 1021 TTGGTGCACC CCCCCTTTAG AGGGAGCATA TGGTGATCTG TTAGAGACGA GACTTAAACC
1081 AGGATCCCGG CCATCATTCT GAATTTTTTT TGC
//
Primer: SV40pA3'Element Reverse (Pr0108)
Region: 3’LTR
Sequence file: RDB14501_A6Lee.seq check
>D04073A1_A6Le_1_SV40pA3element Reverse_E04_13_ABI24.ab1
    1 GTACTCAGTG GGCGGACCTA TGGAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC
   61 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA
  121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG
  181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG
  241 TTGGCCGATT CATTAATGCA ACTATGGCCA TTTAATGTAA ATACTTAAGA AAAAAAACCA
  301 AATTAATTTT GATACATGCT GCATGTGAAA GACCCCCGCT GACGGGTAGT CAATCACTCA
  361 GAGGAGACCC TCCCAAGGAA CAGCGAGACC ACAAGTCGGA TGCAACTGCA AGAGGGTTTA
  421 TTGGATACAC GGGTACCCGG GCGACTCAGT CAATCGGAGG ACTGGCGCCC CGAGTGAGGG
  481 GTTGTGGGCT CTTTTATTGA GCTCGGGGAG CAGAAGCGCG CGAACAGAAG CGAGAAGCGA
  541 ACTGATTGGT TAGTTCAAAT AAGGCACAGG GTCATTTCAG GTCCTTGGGG CACCCTGGAA
  601 ACATCTGATG GTTCTCTAGA AACTGCTGAG GGCTGGACCG CATCTGGGGA CCATCTGTTC
  661 TTGGCCCTGA GCCGGGGCAG GAACTGCTTA CCACAGATAT CCTGTTTGGC CCATATTCAG
  721 CTGTTCCATC TGTTCTTGGC CCTGAGCCGG GGCAGGAACT GCTTACCACA GATATCCTGT
  781 TTGGCCCATA TTCAGCTGTT CCATCTGTTC CTGACCTTGA TCTGAACTTC TCTATTCTCA
  841 GTTATGTATT TTTTCCATGC CTTGCAAAAT GGCGTTACTT AAGCTAGCTT GCCAAACCTA
  901 CAGGTGGGGG TCTTTCATTC CCCCCTTTTT TCTGGAGACT AAATAAAATC TTTTTATTTT
  961 ATCGTCGACC ACTGTGCTGG CGGCCGGGCC CGCTTTACTT GTACAGCTCG TCCATGCCGA
 1021 GGAGTGATTC CCGGCGGCGT TCATCGAACT TCCAGCAAGA ACCAATGTGA ATCGCGCATC
1081 TCGATAGGGA TTCTTTGGCT CAGGCCGGAA CCTGGGGGGT G
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827. [link to RRC of NBRP]