Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMXs-IRES-GFP_IgM

Retroviral expression vector of mouse Immunoglobulin M (IgM).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested. Additional terms and conditions: Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 （Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016） を引用し、寄託者への謝辞の表明を必要とする。 付加的提供条件： pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14501_A6Lep1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5' (Pr0061) Region: pol region, insert 5' Sequence file: RDB14501_A6Lea.seq
>D04073A1_A6Le_1_pBABE5'_A04_01_ABI24.ab1     1 GCGATATGGC ATATGAGATC TTATATGGGG CACCCCCGCC CCTTGTAAAC TTCCCTGACC    61 CTGACATGAC AAGAGTTACT AACAGCCCCT CTCTCCAAGC TCACTTACAG GCTCTCTACT   121 TAGTCCAGCA CGAAGTCTGG AGACCTCTGG CGGCAGCCTA CCAAGAACAA CTGGACCGAC   181 CGGTGGTACC TCACCCTTAC CGAGTCGGCG ACACAGTGTG GGTCCGCCGA CACCAGACTA   241 AGAACCTAGA ACCTCGCTGG AAAGGACCTT ACACAGTCCT GCTGACCACC CCCACCGCCC   301 TCAAAGTAGA CGGCATCGCA GCTTGGATAC ACGCCGCCCA CGTGAAGGCT GCCGACCCCG   361 GGGGTGGACC ATCCTCTAGA CTGCCGGATC CCAGTGTGGT GGTACGGGAA TTCACACAGG   421 ACCTCACCAT GGGATGGAGC TGTATCATGC TCTTCTTGGC AGCAACAGCT ACAGGTGTCC   481 ACTCCCAGGT CCAACTGCAG CAGCCTGGGG CTGAGCTTGT GAAGCCTGGG GCTTCAGTGA   541 AGCTGTCCTG CAAGGCTTCT GGCTACACCT TCACCAGCTA CTTGATGCAC TGGGTGAAGC   601 AGAGGCCTGG ACGAGGCCTT GAGTGGATTG GAAGGATTGA TCCTAATAGT GGTGGTACTA   661 AGTACAATGA GAAGTTCAAG AGCAAGGCCA CACTGACTGT AGACAAACCC TCCAGCACAG   721 CCTACATGCA GCTCAGCAGC CTGACATCTG AGGACTCTGC GGTCTATTAT TGTGCAAGAT   781 ATGATTACTA CGGTAGTAGC TACTTTGACT ACTGGGGCCA AGGCACCACT CTCACAGTCT   841 CGAGTAGTCA GTCCTTCCCA AATGTCTTCC CCCTCGTCTC CTGCGAGAGC CCCCCTGTCT   901 GATAAGAATC TGGTGGCCAT GGGCTGCCTG GCCCGGGACT TCCTGCCCAG CACCATTTCC   961 TTCACCTGGA ACTACCAGAA CAACACTGAA AGTCATCCAG GGGTATCAGA ACCCTTCCCA  1021 ACACCTGAAG ACAGGGGGCA GTACCTAGCC AACATCGCAA GTGATGGCTG TCTCCTAGAG 1081 CCATCTCAAG ATCAGATGGA GTTACCTGGG GATTGCCGA //
Primer: IRES-R (Pr0037) Region: IRES, insert 3' Sequence file: RDB14501_A6Leb.seq
>D04073A1_A6Le_1_IRES-R_A02_02_ABI24.ab1     1 GCATCAGCGG GCTTCGGCCA GTACGTTAGG GGGGGGGGAG GGAGAGGGGG GGGGGGGGCG    61 GAATTTACGT AGCGGCCGCT CATTTTACCT TGAACAGGGT GACGGTGGTG CTGTAGAAGA   121 GGCTCAGGAG GAAGAGGACG ATGAAGGTGG AGGCAGTGGT CCACAGGTTC TCAAAGCCTT   181 CCTCCTCAGC ATTCACCTCC CCCTCAGTGG ACTTGTCCAC GGTCCTCTCG GTCACCAGGT   241 GTGGCAGGGC CTCGTGGCCT ACAACACAGG TATAGGTCTC TCCGGAGTTC CATTCCTCCT   301 CTGTCACAGT CAGGATGCTG TGGGTAAAGT AGAAGCCTGG GGCCCCAGGC TCTGGCATCG   361 GGGCACTGGT CACATACTTC TCTTGGGGCA AGAGTTGCCC TCTCTGAAGC CACTGCACAC   421 TGATGTCTGC AGGAGAGAAG CCCTTCACCA GGCAGGTGAC TGTGGCTGAC TCCCTCAGGT   481 TCAGTTGCTC ACGAGCTGGT GGCAGCAGGT ACACAGCAGG TGGATGTTTG TGCACCTCAT   541 TGGGTTTTGA GATGAATTTC TTCTGTGGTG AAGGCAGATC CCTGTGAGTC ACAGTACACA   601 CAAATTCCTT CCTGTTATTC CAGTCTTCCA CACAAACACT AGCCACACCC TTAGCACTGA   661 AGGTGCCATT GGGATGGCTT TCCATGATTT TAATTTTGGT TTCCAGTGGT TCACCACTTT   721 GAGAAGCCCA GGAGATATTC AGGGTTTCAT AGGTTGCCAG GTTTGAGACC AGACAGGTCA   781 GGTTAGCGGA CTTGCTGAGG AAGATGTCGG CAAAGGAGGG GGGGATGGTG AAGGTTAGGA   841 TGTCTGTGGA GGGACTGGCA GCACATGTGG AGGACACGTT CTTCAAGAAG GTGAGACCCC   901 TGTGATCCAC ACGGCAGTGT ACACATTTCA GGTTCAGCCA GTCGATTTCA GAGATGGTAG   961 TGTGCTATGA CCCTTGTAGG TTGGGGTGTG ATCCTTTGTT CTCGATGTCA CGAATCTGTG  1021 TGAGGCAGAT CCACGAGCCT CCCATCATAG CAGATACCTG GATCGGATTG AGTGAAGTTG 1081 ATGCTGCGAT GATTAGACCT GCTGAGTGCA GGCTAGAAGA AG //
Primer: IRES-F (Pr0036) Region: IRES, EGFP Sequence file: RDB14501_A6Lec.seq
>D04073A1_A6Le_1_IRES-F_C04_07_ABI24.ab1     1 GGCAAGCCTT CATGTGTTTA GTCGAGGTTA AAAAACGTCT AGGCCCCCCG AACCACGGGG    61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC   121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC   181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG   241 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG   301 ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC   361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC   421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG   481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC   541 TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC   601 TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG   661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG   721 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG   781 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAAGCGG CCGGCCGCCA   841 GCACAGTGGT CGACGATAAA ATAAAAGATT TTATTTAGTC TCCAGAAAAA GGGGGGAATG   901 AAAGACCCCA CCTGTAGGTT TGGCAAGCTA GCTTAAGTAA CGCCATTTTT GCAAAGGCAT   961 GGGAAAAAAT ACATAAACTG AGAATAGGAG GAAGTTTCAG GATCAAAGGT CATGAACAGA  1021 TGGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTTC CTGCCCCGCT 1081 CAGCTAGACA GATGGAACAG CTGAAATAAT GGGGGCCCA //
Primer: Amp_R (Pr0008) Region: AmpR pro, 5’LTR, MMLV psi Sequence file: RDB14501_A6Led.seq
>D04073A1_A6Le_1_Amp_R_D04_10_ABI24.ab1     1 CTTCATTTTC ATTATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA    61 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG   121 CCACCTGCAT AATGAAAGAC CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT   181 TTTGCAAGGC ATGGAAAAAT ACATAACTGA GAATAGAAAA GTTCAGATCA AGGTCAGGAA   241 CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG   301 GCTCAGGGCC AAGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA   361 GCAGTTCCTG CCCCGGCTCA GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCAGCCCTC   421 AGCAGTTTCT AGAGAACCAT CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT   481 GTGCCTTATT TGAACTAACC AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC   541 CCCGAGCTCA ATAAAAGAGC CCACAACCCC TCACTCGGCG CGCCAGTCCT CCGATTGACT   601 GAGTCGCCCG GGTACCCGTG TATCCAATAA ACCCTCTTGC AGTTGCATCC GACTTGTGGT   661 CTCGCTGTTC CTTGGGAGGG TCTCCTCTGA GTGATTGACT ACCCGTCAGC GGGGGTCTTT   721 CATTTGGGGG CTCGTCCGGG ATCGGGAGAC CCCTGCCCAG GGACCACCGA CCCACCACCG   781 GGAGGTAAGC TGGCCAGCAA CTTATCTGTG TCTGTCCGAT TGTCTAGTGT CTATGACTGA   841 TTTTATGCGC CTGCGTCGGT ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG   901 TGGAACTGAC GAGTTCGGAA CACCCGGGCC GCAACCCTGG GAGACGTCCC AGGGACTTCG   961 GGGGGCCCGT TTTTTGTGGC CCGACCTGAG TCCAAAAAAT CCCGATCGTT TTGGGACTCT  1021 TTGGTGCACC CCCCCTTTAG AGGGAGCATA TGGTGATCTG TTAGAGACGA GACTTAAACC 1081 AGGATCCCGG CCATCATTCT GAATTTTTTT TGC //
Primer: SV40pA3'Element Reverse (Pr0108) Region: 3’LTR Sequence file: RDB14501_A6Lee.seq
>D04073A1_A6Le_1_SV40pA3element Reverse_E04_13_ABI24.ab1     1 GTACTCAGTG GGCGGACCTA TGGAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC    61 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA   121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG   181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG   241 TTGGCCGATT CATTAATGCA ACTATGGCCA TTTAATGTAA ATACTTAAGA AAAAAAACCA   301 AATTAATTTT GATACATGCT GCATGTGAAA GACCCCCGCT GACGGGTAGT CAATCACTCA   361 GAGGAGACCC TCCCAAGGAA CAGCGAGACC ACAAGTCGGA TGCAACTGCA AGAGGGTTTA   421 TTGGATACAC GGGTACCCGG GCGACTCAGT CAATCGGAGG ACTGGCGCCC CGAGTGAGGG   481 GTTGTGGGCT CTTTTATTGA GCTCGGGGAG CAGAAGCGCG CGAACAGAAG CGAGAAGCGA   541 ACTGATTGGT TAGTTCAAAT AAGGCACAGG GTCATTTCAG GTCCTTGGGG CACCCTGGAA   601 ACATCTGATG GTTCTCTAGA AACTGCTGAG GGCTGGACCG CATCTGGGGA CCATCTGTTC   661 TTGGCCCTGA GCCGGGGCAG GAACTGCTTA CCACAGATAT CCTGTTTGGC CCATATTCAG   721 CTGTTCCATC TGTTCTTGGC CCTGAGCCGG GGCAGGAACT GCTTACCACA GATATCCTGT   781 TTGGCCCATA TTCAGCTGTT CCATCTGTTC CTGACCTTGA TCTGAACTTC TCTATTCTCA   841 GTTATGTATT TTTTCCATGC CTTGCAAAAT GGCGTTACTT AAGCTAGCTT GCCAAACCTA   901 CAGGTGGGGG TCTTTCATTC CCCCCTTTTT TCTGGAGACT AAATAAAATC TTTTTATTTT   961 ATCGTCGACC ACTGTGCTGG CGGCCGGGCC CGCTTTACTT GTACAGCTCG TCCATGCCGA  1021 GGAGTGATTC CCGGCGGCGT TCATCGAACT TCCAGCAAGA ACCAATGTGA ATCGCGCATC 1081 TCGATAGGGA TTCTTTGGCT CAGGCCGGAA CCTGGGGGGT G //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl