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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pT7-hCas9-mC-polyA

Cas9-poly(A) expressing improved plasmid.

Catalog number RDB14423
Resource name pT7-hCas9-mC-polyA
Alternative name T7-NLS-hCAS9-Cdt1-NLS-pA
Clone info. This version contains no FLAG-tag. If you want to have a FLAG-tag version, please order RDB14604.
Comment Constructed from T7-NLS hCas9-pA (RDB13130, Yoshimi, K., Nat. Commun. 7: 10431, 2016. PMID 26786405).
Vector backbone pcDNA3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Streptococcus pyogenes Cas9 genomic DNA Mouse Cdt1 cDNA
Depositor|Developer DNA Bank, |
Other clones in our bank

External Database
Streptococcus pyogenes Cas9 Mouse Cdt1

        Reference sequence
          
         
        Sequence (full) RDB14423zzt02.seq compiled by us

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Mizuno-Iijima, S., et al., Methods 191: 23-31 (2021)) is requested. The RECIPIENT agrees to expressly describe the acknowledgment of the RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication.
        Ordering Please visit Information of Request for Distribution.[link] 
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        Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
        Remarks
        The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
        提供案内 (日本国内) [open/close]

        提供条件 本件リソースの使用は学術機関での学術研究に限る。利用者は、研究成果の公表にあたって寄託者の指定する文献 (Mizuno-Iijima, S., et al., Methods 191: 23-31 (2021)) を引用する。本件リソースを使用して研究成果を発表する場合、理研BRCから提供されたことを明示する。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        専用MTA(CRISPR/Cas9内包遺伝子材料専用 非営利学術目的)をお使いください [Word]
        備考
        本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB14423 pT7-hCas9-mC-polyA DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Original reference

        original Mizuno-Iijima, S., Efficient production of large deletion and gene fragment knock-in mice mediated by genome editing with Cas9-mouse Cdt1 in mouse zygotes. Methods 191: 23-31 (2021). PMID 32334080.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Genome Editing (English text)
        Featured content Genome Editing (Japanese text)

        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB14423_A6L1p1-1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: CMV_Forward
        Region: CMV pro, T7 pro, SV40 NLS, insert5'(Cas9 5')
        Sequence file: RDB14423_A6L1a.seq check
        >14423_14423_B2FgF_1_CMV_Forward_F03_18_ABI24.ab1
            1 NNNNNNNNNN NNNNNNTTGN NGCNNNTGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
           61 AAGCAGAGCT CGTTTAGTGA ACCGTCAGAT CGCCTGGAGA CGCCATCCAC GCTGTTTTGA
          121 CCTCCATAGA AGACACCGGG ACCGATCCAG CCTCCGGACT CTAGTAATAC GACTCACTAT
          181 AGGGAGAGCC GCCACCATGG CCCCAAAGAA GAAGCGGAAG GTCGGTATCC ACGGAGTCCC
          241 AGCAGCCGAC AAGAAGTACT CCATTGGGCT CGATATCGGC ACAAACAGCG TCGGCTGGGC
          301 CGTCATTACG GACGAGTACA AGGTGCCGAG CAAAAAATTC AAAGTTCTGG GCAATACCGA
          361 TCGCCACAGC ATAAAGAAGA ACCTCATTGG CGCCCTCCTG TTCGACTCCG GGGAGACGGC
          421 CGAAGCCACG CGGCTCAAAA GAACAGCACG GCGCAGATAT ACCCGCAGAA AGAATCGGAT
          481 CTGCTACCTG CAGGAGATCT TTAGTAATGA GATGGCTAAG GTGGATGACT CTTTCTTCCA
          541 TAGGCTGGAG GAGTCCTTTT TGGTGGAGGA GGATAAAAAG CACGAGCGCC ACCCAATCTT
          601 TGGCAATATC GTGGACGAGG TGGCGTACCA TGAAAAGTAC CCAACCATAT ATCATCTGAG
          661 GAAGAAGCTT GTAGACAGTA CTGATAAGGC TGACTTGCGG TTGATCTATC TCGCGCTGGC
          721 GCATATGATC AAATTTCGGG GANACTTCCT CATCGAGGGG GANCTGAACC CAGACAACAG
          781 CGATGTCGAC AAACTCTTTA TCCAACTGGT TCAGANTTAC AATCAGCTTT TCGAAGAGAA
          841 NCCGATCNAC GCATCCGGAG TTGANGCCNA AGCAATCCTG ANCGCTANGN TGTNNNANTC
          901 CNGGCGGCTC GAAAANCNCA TCGCACAGCN CCCNGGGGAN AANAANAACG GNCNGNTTGG
          961 TANTCNNANC GCCCTGNCNC TCGGGNNGAN CCCCNACTTT AAATCTNANT TCGNNCNNNN
        1021 NNANNNNNNN NNN
        //
        Primer: HSV-pA_R
        Region: SV40 NLS, insert3' (ccdt1, Cas9 3')
        Sequence file: RDB14423_A6L1b.seq check
        >14423_14423_B2FgF_1_HSV-pA_R_G03_21_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN GNGTTNNNGT TAGCCTCCCC CGTTNAAACT CNTTACTAAC
           61 CGGTAGGGAT CGAACCCTTT CACACCTTCC TCTTCTTCTT GGGGTCAGCC CTGCTGACGG
          121 AGTCCTCTGA GGGGATCTTG CCCGGCTGTT GACCAACATA AACAGTTGTA CTCTTTGTTC
          181 GCTTGACGGG GGATGGGCAA ACGGGGGAGC CAGGGTCAGC TGGAGGGCTA GGGTCAGCTG
          241 GAGGGCTGGG CTCAGCTGGG GAACTGGGCT CAGGCAGAGA ACTGGGGCAG GAGTCCAATC
          301 CAGGCAGCCG TAACCTCCGG CGCGCGAGCG GCGCGGGCTG GTCACTCCCG GGTTCGGCTG
          361 GGGGCCGGGC GCGCTTGCGG CTGCTGCTCC GGGTGAACGC AGGAGCCACG AGGCCACCAG
          421 GGCTCGGGTC TCCACCGAGC TGAGAGAGGT CGATTCTTGT TTCATAGAGC CCCGTAATTG
          481 ACTGATGAAT CAGTGTGGCG TCCAGGACCT CCTTTGTAGA GGTGTACCGC TTTCTGTCTA
          541 TGGTGGTGTC GAAGTACTTG AAGGCTGCAG GCGCGCCCAA GTTGGTCAGA GTAAACAAGT
          601 GGATAATGTT TTCTGCCTGC TCCCTGATGG GCTTATCCCT GTGCTTATTG TAAGCAGAAA
          661 GCACCTTATC GAGGTTAGCG TCGGCGAGGA TCACTCTTTT GGAGAATTCG CTTATTTGCT
          721 CGATGATCTC ATCAAGGTAG TGTTTGTGTT GTTCCACGAA CAGCTGCTTC TGCTCATTAT
          781 CTTCGGGAGA CCCTTTGAGC TTTTCATAGT GGCTGGNCAG ATACAAGAAA TTAACGTATT
          841 TAGAGGGCAG TGCCAGCTCG TTACNTTTCT GCAGCTCGCC CGCACTAGCG AGCATTCGTT
          901 TCCGGNCGTT TTCAAGCTCN AAGANAGAGN ANTTNGGAAG CTNNNTGATG AGGNCTTTTT
          961 TTGANNNCTT TNTATCCNTT CNNNNCNAGA AANTCNNNGG GGNNTTTTTT TCNAANNNNG
        1021 NTNNNNNNNN NANNGNGANN NCNNNNNNNN NN
        //
        Primer: f1ori_F
        Region: f1 ori, pA
        Sequence file: RDB14423_A6L1c.seq check
        >14423_14423_B2FgF_1_f1ori_F_H03_24_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNGN ANNGNAGCGG TCACGCTGCG CGTAACCACC ACACCCGCCG
           61 CGCTTAATGC GCCGCNACNG GGCGCGTGGG GATACCCCCT AGAGCCCCAG CTGCGCAGAT
          121 CTGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC
          181 TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG
          241 GTGGGGTATC GATGTACAGC TAGCTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
        301 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTN NNNNNNNNN
        //
        Primer: SV40pro_F
        Region: SV40pro_ori, NeoR
        Sequence file: RDB14423_A6L1d.seq check
        >D04203A5_A6L1_1_SV40pro_F_B06_06_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTG NNGANGCTTT TTTGGAGGCC TAGGCTTTTG
           61 CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG
          121 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA
          181 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT
          241 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT
          301 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG
          361 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT
          421 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC
          481 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC
          541 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA
          601 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG
          661 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT
          721 GGTGGAAAAT GGNCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGANCG
          781 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC
          841 TGANNGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA
          901 TCGCCTNNTT GACGAGTTCT TCTGANCGGG ANNCTNGGGN NCNCGAANTG ANNNANCNAG
          961 CGANGCCNNA NCNNGCCNTC ACNANATTTC NANNNCNNNN NNNNCNTNNN ATGNAANNNN
        1021 NGGNNNNNNN NNN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Mizuno-Iijima, S., Efficient production of large deletion and gene fragment knock-in mice mediated by genome editing with Cas9-mouse Cdt1 in mouse zygotes. Methods 191: 23-31 (2021). PMID 32334080.

        2022.08.12

        GNP_filter3_RDBDEP_html_220812.pl