Please review the QC test results indicated by icon below as well as clone information before placing your order.

pT7-hCas9-mC-polyA

Cas9-poly(A) expressing improved plasmid.

Sequence (full)	RDB14423zzt02.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Mizuno-Iijima, S., et al., Methods 191: 23-31 (2021)) is requested. The RECIPIENT agrees to expressly describe the acknowledgment of the RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Remarks	The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	本件リソースの使用は学術機関での学術研究に限る。利用者は、研究成果の公表にあたって寄託者の指定する文献 (Mizuno-Iijima, S., et al., Methods 191: 23-31 (2021)) を引用する。本件リソースを使用して研究成果を発表する場合、理研BRCから提供されたことを明示する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
備考	本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

References and tips

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14423_A6L1p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro, T7 pro, SV40 NLS, insert5'(Cas9 5') Sequence file: RDB14423_A6L1a.seq
>14423_14423_B2FgF_1_CMV_Forward_F03_18_ABI24.ab1     1 NNNNNNNNNN NNNNNNTTGN NGCNNNTGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT    61 AAGCAGAGCT CGTTTAGTGA ACCGTCAGAT CGCCTGGAGA CGCCATCCAC GCTGTTTTGA   121 CCTCCATAGA AGACACCGGG ACCGATCCAG CCTCCGGACT CTAGTAATAC GACTCACTAT   181 AGGGAGAGCC GCCACCATGG CCCCAAAGAA GAAGCGGAAG GTCGGTATCC ACGGAGTCCC   241 AGCAGCCGAC AAGAAGTACT CCATTGGGCT CGATATCGGC ACAAACAGCG TCGGCTGGGC   301 CGTCATTACG GACGAGTACA AGGTGCCGAG CAAAAAATTC AAAGTTCTGG GCAATACCGA   361 TCGCCACAGC ATAAAGAAGA ACCTCATTGG CGCCCTCCTG TTCGACTCCG GGGAGACGGC   421 CGAAGCCACG CGGCTCAAAA GAACAGCACG GCGCAGATAT ACCCGCAGAA AGAATCGGAT   481 CTGCTACCTG CAGGAGATCT TTAGTAATGA GATGGCTAAG GTGGATGACT CTTTCTTCCA   541 TAGGCTGGAG GAGTCCTTTT TGGTGGAGGA GGATAAAAAG CACGAGCGCC ACCCAATCTT   601 TGGCAATATC GTGGACGAGG TGGCGTACCA TGAAAAGTAC CCAACCATAT ATCATCTGAG   661 GAAGAAGCTT GTAGACAGTA CTGATAAGGC TGACTTGCGG TTGATCTATC TCGCGCTGGC   721 GCATATGATC AAATTTCGGG GANACTTCCT CATCGAGGGG GANCTGAACC CAGACAACAG   781 CGATGTCGAC AAACTCTTTA TCCAACTGGT TCAGANTTAC AATCAGCTTT TCGAAGAGAA   841 NCCGATCNAC GCATCCGGAG TTGANGCCNA AGCAATCCTG ANCGCTANGN TGTNNNANTC   901 CNGGCGGCTC GAAAANCNCA TCGCACAGCN CCCNGGGGAN AANAANAACG GNCNGNTTGG   961 TANTCNNANC GCCCTGNCNC TCGGGNNGAN CCCCNACTTT AAATCTNANT TCGNNCNNNN 1021 NNANNNNNNN NNN //
Primer: HSV-pA_R (Pr0949) Region: SV40 NLS, insert3' (ccdt1, Cas9 3') Sequence file: RDB14423_A6L1b.seq
>14423_14423_B2FgF_1_HSV-pA_R_G03_21_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GNGTTNNNGT TAGCCTCCCC CGTTNAAACT CNTTACTAAC    61 CGGTAGGGAT CGAACCCTTT CACACCTTCC TCTTCTTCTT GGGGTCAGCC CTGCTGACGG   121 AGTCCTCTGA GGGGATCTTG CCCGGCTGTT GACCAACATA AACAGTTGTA CTCTTTGTTC   181 GCTTGACGGG GGATGGGCAA ACGGGGGAGC CAGGGTCAGC TGGAGGGCTA GGGTCAGCTG   241 GAGGGCTGGG CTCAGCTGGG GAACTGGGCT CAGGCAGAGA ACTGGGGCAG GAGTCCAATC   301 CAGGCAGCCG TAACCTCCGG CGCGCGAGCG GCGCGGGCTG GTCACTCCCG GGTTCGGCTG   361 GGGGCCGGGC GCGCTTGCGG CTGCTGCTCC GGGTGAACGC AGGAGCCACG AGGCCACCAG   421 GGCTCGGGTC TCCACCGAGC TGAGAGAGGT CGATTCTTGT TTCATAGAGC CCCGTAATTG   481 ACTGATGAAT CAGTGTGGCG TCCAGGACCT CCTTTGTAGA GGTGTACCGC TTTCTGTCTA   541 TGGTGGTGTC GAAGTACTTG AAGGCTGCAG GCGCGCCCAA GTTGGTCAGA GTAAACAAGT   601 GGATAATGTT TTCTGCCTGC TCCCTGATGG GCTTATCCCT GTGCTTATTG TAAGCAGAAA   661 GCACCTTATC GAGGTTAGCG TCGGCGAGGA TCACTCTTTT GGAGAATTCG CTTATTTGCT   721 CGATGATCTC ATCAAGGTAG TGTTTGTGTT GTTCCACGAA CAGCTGCTTC TGCTCATTAT   781 CTTCGGGAGA CCCTTTGAGC TTTTCATAGT GGCTGGNCAG ATACAAGAAA TTAACGTATT   841 TAGAGGGCAG TGCCAGCTCG TTACNTTTCT GCAGCTCGCC CGCACTAGCG AGCATTCGTT   901 TCCGGNCGTT TTCAAGCTCN AAGANAGAGN ANTTNGGAAG CTNNNTGATG AGGNCTTTTT   961 TTGANNNCTT TNTATCCNTT CNNNNCNAGA AANTCNNNGG GGNNTTTTTT TCNAANNNNG 1021 NTNNNNNNNN NANNGNGANN NCNNNNNNNN NN //
Primer: f1ori_F (Pr0280) Region: f1 ori, pA Sequence file: RDB14423_A6L1c.seq
>14423_14423_B2FgF_1_f1ori_F_H03_24_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGN ANNGNAGCGG TCACGCTGCG CGTAACCACC ACACCCGCCG    61 CGCTTAATGC GCCGCNACNG GGCGCGTGGG GATACCCCCT AGAGCCCCAG CTGCGCAGAT   121 CTGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC   181 TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG   241 GTGGGGTATC GATGTACAGC TAGCTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT 301 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTN NNNNNNNNN //
Primer: SV40pro_F (Pr0110) Region: SV40pro_ori, NeoR Sequence file: RDB14423_A6L1d.seq
>D04203A5_A6L1_1_SV40pro_F_B06_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTG NNGANGCTTT TTTGGAGGCC TAGGCTTTTG    61 CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG   121 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA   181 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT   241 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT   301 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG   361 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT   421 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC   481 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC   541 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA   601 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG   661 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT   721 GGTGGAAAAT GGNCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGANCG   781 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC   841 TGANNGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA   901 TCGCCTNNTT GACGAGTTCT TCTGANCGGG ANNCTNGGGN NCNCGAANTG ANNNANCNAG   961 CGANGCCNNA NCNNGCCNTC ACNANATTTC NANNNCNNNN NNNNCNTNNN ATGNAANNNN 1021 NGGNNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl