Resource data sheet
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Cas9-poly(A) nickase with enhanced specificity.

Catalog number RDB14421
Resource name T7-NLS-hCAS9(D10A_ver1.1)-NLS-pA
Clone info. Cas9 expressing plasmid. Induction with CMV or T7 promoter. Applicable for both direct transfection into mammalian cells and T7 in-vitro translation. Harboring nickase mutation (D10A). Enhanced specificity version mutations (SpCas9-K848A,K1003A,R1060A; Slaymaker IM et al., science 351(6268), 84-88, 2016: PMID26628643) was also introduced.
Comment Constructed from T7-NLS hCas9-pA (RDB13130, Yoshimi, K., Nat. Commun. 7: 10431, 2016. PMID 26786405).
Vector backbone pcDNA3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Streptococcus pyogenes Cas9 genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Streptococcus pyogenes Cas9

          Reference sequence
          Sequence (full) RDB14421zzt02.seq compiled by us

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. The RECIPIENT agrees to expressly describe the acknowledgement of the RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication.
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          Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
          The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
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          提供条件 本件リソースの使用は学術機関での学術研究に限る。本件リソースを使用して研究成果を発表する場合、理研BRCから提供されたことを明示する。
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          提供依頼書 [Word]
          専用MTA(CRISPR/Cas9内包遺伝子材料専用 非営利学術目的)をお使いください [Word]
          本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB14421 T7-NLS-hCAS9(D10A_ver1.1)-NLS-pA Under QC test. Please contact us. DNA solution

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          References and tips

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Genome Editing (English text)
          Featured content Genome Editing (Japanese text)

          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          Original, user report and related articles