Please review the QC test results indicated by icon below as well as clone information before placing your order.

T7-NLS-hCAS9(ver1.1)-NLS-pA

Cas9-poly(A) with enhanced specificity.

Sequence (full)	RDB14420zzt02.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. The RECIPIENT agrees to expressly describe the acknowledgement of the RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Remarks	The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	本件リソースの使用は学術機関での学術研究に限る。本件リソースを使用して研究成果を発表する場合、理研BRCから提供されたことを明示する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
備考	本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14420_A6L1p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro, SV40 NLS, insert 5' Sequence file: RDB14420_A6L1a.seq
>D04203A2_A6L1_1_CMV_Forward_C06_03_ABI08.ab1     1 NNNNNNNNNN NNNCNNNNTT GNNGCNNNGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA    61 TAAGCAGAGC TCGTTTAGTG AACCGTCAGA TCGCCTGGAG ACGCCATCCA CGCTGTTTTG   121 ACCTCCATAG AAGACACCGG GACCGATCCA GCCTCCGGAC TCTAGTAATA CGACTCACTA   181 TAGGGAGAGC CGCCACCATG GCCCCAAAGA AGAAGCGGAA GGTCGGTATC CACGGAGTCC   241 CAGCAGCCGA CAAGAAGTAC TCCATTGGGC TCGATATCGG CACAAACAGC GTCGGCTGGG   301 CCGTCATTAC GGACGAGTAC AAGGTGCCGA GCAAAAAATT CAAAGTTCTG GGCAATACCG   361 ATCGCCACAG CATAAAGAAG AACCTCATTG GCGCCCTCCT GTTCGACTCC GGGGAGACGG   421 CCGAAGCCAC GCGGCTCAAA AGAACAGCAC GGCGCAGATA TACCCGCAGA AAGAATCGGA   481 TCTGCTACCT GCAGGAGATC TTTAGTAATG AGATGGCTAA GGTGGATGAC TCTTTCTTCC   541 ATAGGCTGGA GGAGTCCTTT TTGGTGGAGG AGGATAAAAA GCACGAGCGC CACCCAATCT   601 TTGGCAATAT CGTGGACGAG GTGGCGTACC ATGAAAAGTA CCCAACCATA TATCATCTGA   661 GGAAGAAGCT TGTAGACAGT ACTGATAAGG CTGACTTGCG GTTGATCTAT CTCGCGCTGG   721 CGCATATGAT CAAATTTCGG GGACACTTCC TCATCGAGGG GGANCTGAAC CCAGACAACA   781 GCGNTGTCGA CNAACTCTTT ATCCNACTGG NTCNNANTTA CAATCAGCTT TTCGAAGANN   841 AACCCGATCA ACGCATCCNG AGTTGANGCC NAAGCNATCC TGANCGCTNN GCTGNNNNAA   901 TCCCNGNCGG NTCNAAAANC TCNTCGCACA NCTCCCNGGG GNNNNNAAGN NNNGNCNNNT 961 NNNNNNNNNN //
Primer: HSV_TK_pA_R (Pr0137) Region: SV40 NLS, insert 3' Sequence file: RDB14420_A6L1b.seq
>D04203A2-1_A6L1_1_HSV_TK_pA_R_A02_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNTG NCNNNTTCCG TGTTTNNGTT AGCCTCCCCC GTTTAAACTC    61 ATTACTAACC GGTAGGGATC GAACCCTTTC ACACCTTCCT CTTCTTCTTG GGGTCAGCCC   121 TGCTGTCTCC ACCGAGCTGA GAGAGGTCGA TTCTTGTTTC ATAGAGCCCC GTAATTGACT   181 GATGAATCAG TGTGGCGTCC AGGACCTCCT TTGTAGAGGT GTACCGCTTT CTGTCTATGG   241 TGGTGTCGAA GTACTTGAAG GCTGCAGGCG CGCCCAAGTT GGTCAGAGTA AACAAGTGGA   301 TAATGTTTTC TGCCTGCTCC CTGATGGGCT TATCCCTGTG CTTATTGTAA GCAGAAAGCA   361 CCTTATCGAG GTTAGCGTCG GCGAGGATCA CTCTTTTGGA GAATTCGCTT ATTTGCTCGA   421 TGATCTCATC AAGGTAGTGT TTGTGTTGTT CCACGAACAG CTGCTTCTGC TCATTATCTT   481 CGGGAGACCC TTTGAGCTTT TCATAGTGGC TGGCCAGATA CAAGAAATTA ACGTATTTAG   541 AGGGCAGTGC CAGCTCGTTA CCTTTCTGCA GCTCGCCCGC ACTAGCGAGC ATTCGTTTCC   601 GGCCGTTTTC AAGCTCAAAG AGAGAGTACT TGGGAAGCTT AATGATGAGG TCTTTTTTGA   661 CCTCTTTATA TCCTTTCGCC TCGAGAAAGT CGATGGGGTT TTTTTCGAAG CTTGATCGCT   721 CCATGATTGT GATGCCCAGC AGTTCCTTGA CGCTNNTGAN TTTTTTAGAC TTCCCTTTCT   781 CCACTTNGGC CACNACCAGT ACACTGNAAG CGACTGTANG AGAATCNANT CCGCCGTATT   841 TCTTGGGGNC CNAATCTTTT TTNGCGTGCN ATCAGCTNGN CGCNNTNCCT TTNNGGGANG   901 ANNNTTTCNT NNNNNAANNN NNNNGNCTGN ACNTCGGNNN TTTTANNGAN GNTNNNNNNN 961 NNN //
Primer: hCAS9+2010_F (Pr0425) Region: insert mid (K848A) Sequence file: RDB14420_A6L1c.seq
>D04203A2_A6L1_1_hCAS9+2010_F_D06_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNTNNN NNNNNNCCGA NGGNNTTGCC AACCGGAACT TCATGCAGTT    61 GATCCATGAT GACTCTCTCA CCTTTAAGGA GGACATCCAG AAAGCACAAG TTTCTGGCCA   121 GGGGGACAGT CTTCACGAGC ACATCGCTAA TCTTGCAGGT AGCCCAGCTA TCAAAAAGGG   181 AATACTGCAG ACCGTTAAGG TCGTGGATGA ACTCGTCAAA GTAATGGGAA GGCATAAGCC   241 CGAGAATATC GTTATCGAGA TGGCCCGAGA GAACCAAACT ACCCAGAAGG GACAGAAGAA   301 CAGTAGGGAA AGGATGAAGA GGATTGAAGA GGGTATAAAA GAACTGGGGT CCCAAATCCT   361 TAAGGAACAC CCAGTTGAAA ACACCCAGCT TCAGAATGAG AAGCTCTACC TGTACTACCT   421 GCAGAACGGC AGGGACATGT ACGTGGATCA GGAACTGGAC ATCAATCGGC TCTCCGACTA   481 CGACGTGGAT CATATCGTGC CCCAGTCTTT TCTCGCAGAT GATTCTATTG ATAATAAAGT   541 GTTGACAAGA TCCGATAAAA ATAGAGGGAA GAGTGATAAC GTCCCCTCAG AAGAAGTTGT   601 CAAGAAAATG AAAAATTATT GGCGGCAGCT GCTGAACGCC AAACTGATCA CACAACGGAA   661 GTTCGATAAT CTGACTAAGG CTGAACGAGG TGGCCTGTCT GANTTGGATA AAGCCGGCTT   721 CATCAAAAGG CAGCTTGTTG AGACACGCCA GATCACCAAG CACGTGGCCC AAATTCTCGA   781 NTCACGCATG AACACCANGT ACGATGANAA TGACNAACTG ATTCGAGAGG TGAAAGTTAT   841 TACTCTGAAG TCTAAGCTGG TCTCAGATTT CAGAAAGGAC TTTCNGTTTT ATANNGNGAG   901 AGAGATCAAC NNTTACNNCN NNGCGCATGA NGNCNACCNN NANGCNNNGG TANGNNNNGN 961 NCNTNNTCAA AAAATATCCN NNGNNNNNNN NNN //
Primer: hCAS9+3550_R (Pr0424) Region: insert mid (R1060A, K1003A) Sequence file: RDB14420_A6L1d.seq
>D04203A2_A6L1_1_hCAS9+3550_R_E06_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNGNNNG NGNNGCCCAG CANNTTCCTT GNNGCTTTTG    61 AGTTTTTTAG ACTTCCCTTT CTCCNCTTTG GCCACAACCA GTACACTGTA AGCGACTGTA   121 GGAGAATCGA ATCCGCCGTA TTTCTTGGGG TCCCAATCTT TTTTGCGTGC GATCAGCTTG   181 TCGCTGTTCC TTTTCGGGAG GATACTTTCC TTGGAGAAGC CTCCGGTCTG TACTTCGGTC   241 TTTTTAACGA TGTTCACCTG CGGCATGGAC AGGACCTTCC GGACTGTCGC GAAATCCCTA   301 CCCTTGTCCC ACACGATTTC TCCTGTTTCT CCGTTTGTTT CGATAAGTGG TGCCTTCCGA   361 ATCTCTCCAT TGGCCAGTGT AATCTCGGTC TTGAAAAAAT TCATAATATT GCTGTAAAAG   421 AAGTACTTAG CGGTGGCCTT GCCTATTTCC TGCTCAGACT TTGCGATCAT TTTCCTAACA   481 TCGTACACTT TATAGTCTCC GTAAACAAAT TCAGATTCAA GCGCGGGATA TTTTTTGATA   541 AGTGCAGTGC CTACCACTGC ATTCAGGTAG GCATCATGCG CATGGTGGTA ATTGTTGATC   601 TCTCTCACCT TATAAAACTG AAAGTCCTTT CTGAAATCTG AGACCAGCTT ANACTTCAGA   661 GTANTAACTT TCACCTCTCG AATCAGTTTG TCATTTTCAT CGTACTTGGT GTTCATGCGT   721 GAATCGAGAA TTTGGGCCAC GTGCTTGGTG ATCTGGCGTG TCTCANCNAG CTGCCTTTTG   781 ATGAANCCGG CTTTATCNNA CTCANANAGG CCNCCTCGTT CAGCCTTANT CAGANTATCG   841 AACTTCCGTT GTGTGATCAG TTTGNNNNNN CANCAGCTGN CGCNNANTAA TTTTTTNNNT   901 TTNCNTGANA NNTNCNTCNG AGGGGNANGT TNNNNNCTCN NCCNTCNNNT TTTNNNCNGN 961 ATNNNNGNNN NNNNNTTNNN NATNNNNANN ANNNNNNNNN N //
Primer: SV40pro_ori_F (Pr0731) Region: NeoR Sequence file: RDB14420_A6L1e.seq
>D04203A2_A6L1_1_SV40pro_ori_F_F06_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN GCCTCTGCCT CTGNNCTANT CNNNNNNNAG TGAGGAGGCT    61 TTTTTGGAGG CCTAGGCTTT TGCANANNNC TCCCGGGAGC TTGTATATCC ATTTTCGGAT   121 CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG   181 GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG   241 GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA   301 AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC   361 TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG   421 ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG   481 CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA   541 CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG   601 CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA NGGGCTCGCG CCAGCCGAAC   661 TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG   721 ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC NTCGACTGTG   781 GCCGGCTGGG NGTGGNGGAN CGCTATCANG ACATANCGTT GGCTACCCGN GATATTGCTG   841 AAGANCTNGN CGGCGAANGG GCTGANNNCT TCCTCGTGCT TTNNCNGNAT CNNCNGCTCC   901 CGATTCNNAN CGCATCNNNT TCTATCNNNN TCTNGACNAN TNCNTCNNNN CGGNNNTNNG 961 GGGGNNNNNN NNAANNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl