Resource data sheet


Cas9-poly(A) with enhanced specificity.

Catalog number RDB14420
Resource name T7-NLS-hCAS9(ver1.1)-NLS-pA
Clone info. Cas9 expressing plasmid. Induction with CMV or T7 promoter. Applicable for both direct transfection into mammalian cells and T7 in-vitro translation. Having enhanced specificity.
Constructed from T7-NLS hCas9-pA (RDB13130, Yoshimi, K., Nat. Commun. 7: 10431, 2016. PMID 26786405).
Vector backbone pcDNA3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Streptococcus pyogenes Cas9 genomic DNA
Depositor DNA Bank, |
Other clones in our bank Streptococcus pyogenes (KEGG orthology K09952) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. The RECIPIENT agrees to expressly describe the acknowledgement of the RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication.
Please use the Exclusive MTA (genome editing). [Word]
Distribution information (domestic users) [open/close]

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14420 T7-NLS-hCAS9(ver1.1)-NLS-pA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Genome Editing (English text)
Featured content Genome Editing (Japanese text)


User reports and related articles (go to bottom)

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.