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T7-NLS-hCAS9(D10A)-NLS-pA

Cas9-poly(A) nickase expressing plasmid.

Catalog number RDB14419
Resource name T7-NLS-hCAS9(D10A)-NLS-pA
Clone info. Cas9 expressing plasmid. Induction with CMV or T7 promoter. Applicable for both direct transfection into mammalian cells and T7 in-vitro translation. Harboring nickase mutation (D10A).
Constructed from T7-NLS hCas9-pA (RDB13130, Yoshimi, K., Nat. Commun. 7: 10431, 2016. PMID 26786405).
Vector backbone pcDNA3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Streptococcus pyogenes Cas9 genomic DNA
Depositor DNA Bank, |
 
Other clones in our bank Streptococcus pyogenes (KEGG orthology K09952) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. The RECIPIENT agrees to expressly describe the acknowledgement of the RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14419 T7-NLS-hCAS9(D10A)-NLS-pA DNA solution

Ordering Information [in Japanese] [in English]

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

References


2018.09.20

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