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px330-mC

Expression vector of sgRNA with hSpCas9-Cdt1(mouse) fusion protein.

Catalog number RDB14406
Resource name px330-mC
Clone info. Expression vector of sgRNA with hSpCas9-Cdt1(mouse) fusion protein (aa29-132, fused to Cas9 C-terminus).
Vector backbone pX330-U6-Chimeric_BB-CBh-hSpCas9 (plasmid)
Size of vector backbone 8.5 kb
Selectable markers Ampicillin
Gene/insert name mouse Cdt1 cDNA S. pyogenes Cas9 genomic
Depositor|Developer Sugiyama, Fumihiro | Mizuno, Seiya |
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External Database
mouse Cdt1 S. pyogenes Cas9

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Mizuno-Iijima, S. et al., Methods 191: 23-31 (2021)).
        Ordering Please visit Information of Request for Distribution.[link] 
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        Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
        Remarks
        The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please refer the Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose).
        提供案内 (日本国内) [open/close]

        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Mizuno-Iijima, S. et al., Methods 191: 23-31 (2021))。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        専用MTA(CRISPR/Cas9内包遺伝子材料専用 非営利学術目的)をお使いください [Word]
        備考
        本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。専用MTA(CRISPR/Cas9内包遺伝子材料専用 非営利学術目的)をご参照ください。

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        RDB14406 px330-mC DNA solution

        Ordering Information [in Japanese] [in English]
        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Mizuno-Iijima, S., Efficient production of large deletion and gene fragment knock-in mice mediated by genome editing with Cas9-mouse Cdt1 in mouse zygotes. Methods 191: 23-31 (2021). PMID 32334080.

        Further references such as user reports and related articles (go to bottom)

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        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB14406_A6L1p1-2.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: SV40pA3element Reverse
        Region: U6 pro,gRNA scaffold
        Sequence file: RDB14406_A6L1d.seq check
        >D04199A2_A6L1_1_SV40pA3'element Reverse_D04_10_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNANNGN ANANNNCCNN NCNANCGCGG CCTTTTTACG
           61 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTGAGGG CCTATTTCCC ATGATTCCTT
          121 CATATTTGCA TATACGATAC AAGGCTGTTA GAGAGATAAT TGGAATTAAT TTGACTGTAA
          181 ACACAAAGAT ATTAGTACAA AATACGTGAC GTAGAAAGTA ATAATTTCTT GGGTAGTTTG
          241 CAGTTTTAAA ATTATGTTTT AAAATGGACT ATCATATGCT TACCGTAACT TGAAAGTATT
          301 TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA CACCGGGTCT TCGAGAAGAC
          361 CTGTTTTAGA GCTAGAAATA GCAAGTTAAA ATAAGGCTAG TCCGTTATCA ACTTGAAAAA
          421 GTGGCACCGA GTCGGTGCTT TTTTGTTTTA GAGCTAGAAA TAGCAAGTTA AAATAAGGCT
          481 AGTCCGTTTT TANNNCGTGN NNCNATTCTG CAGACAAATG GCTCTAGAGG TACCCGTTAC
          541 ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC
          601 AATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC
          661 TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA
          721 TGACGGTAAA TGGCCCGCCT GGCATTGTGC CCAGTACATG ACCTTATGGN ANTTTCCTAC
          781 TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GGNCGANGTG AGNCCCACGT
          841 TCTGCTNCAC TCTCCCCATC TCCCCCCCCC TCCCCCNNCC CCCAATTTTG NATTTATTTA
        901 TTTTTTTANN TATTTTTGTG CANNNANNGG GGGCGGGGGG GGGGGGGGGG GN
        //
        Primer: RDB13535#1
        Region: 3XFLAG,SV40 NLS,Cas9 5'
        Sequence file: RDB14406_A6L1e.seq check
        >D04199A2_A6L1_1_RDB13535#1_E04_13_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNTTT TTTTNNGGTT GGNNCGGTGC CNCCATGGNC TATAAGGACC
           61 ACGACGGAGA CTACAAGGAT CATGATATTG ATTACAAAGA CGATGACGAT AAGATGGCCC
          121 CAAAGAAGAA GCGGAAGGTC GGTATCCACG GAGTCCCAGC AGCCGACAAG AAGTACAGCA
          181 TCGGCCTGGA CATCGGCACC AACTCTGTGG GCTGGGCCGT GATCACCGAC GAGTACAAGG
          241 TGCCCAGCAA GAAATTCAAG GTGCTGGGCA ACACCGACCG GCACAGCATC AAGAAGAACC
          301 TGATCGGAGC CCTGCTGTTC GACAGCGGCG AAACAGCCGA GGCCACCCGG CTGAAGAGAA
          361 CCGCCAGAAG AAGATACACC AGACGGAAGA ACCGGATCTG CTATCTGCAA GAGATCTTCA
          421 GCAACGAGAT GGCCAAGGTG GACGACAGCT TCTTCCACAG ACTGGAAGAG TCCTTCCTGG
          481 TGGAAGAGGA TAAGAAGCAC GAGCGGCACC CCATCTTCGG CAACATCGTG GACGAGGTGG
          541 CCTACCACGA GAAGTACCCC ACCATCTACC ACCTGAGAAA GAAACTGGTG GACAGCACCG
          601 ACNAGGCCGA CCTGCGGCTG ATCTATCTGG CCCTGGCCCA CATGATCAAG TTCCGGGGCC
          661 ACTTCCTGAT CGAGGGCGAN CTGAACCCCG ACAACAGCGA CGTGGACAAG CTGTTCATCC
          721 AGCTGGTGCA GACCTACAAC CAGCTGTTCG ANGAAAACCN CATCAACGCC NGCGGCGTGG
          781 ANGCCNAGGC CATCCTGTCT GCCNGANTGA NCAAGANCAG ANGGCTGGAA AATCTGATCG
          841 CCCAGCTGCC CGGCGANAAG AANAATGGNC NGTNCGGAAA NCNGATTGNC NNGAGCCNGG
          901 GNNNGACCCC CNNCTTNNNN ANCNNCTTCN ANNNNNNNNN ANNNNGNNNA NNTGNAGCNN
        961 ANCNANNNAN NNCNTACNNN NANNANCNNN NNNNCNNNNN NGNNNNNNNN NN
        //
        Primer: BGH_rev2
        Region: bGHpA,SV40 NLS,mCdt1,Cas9 3'
        Sequence file: RDB14406_A6L1f.seq check
        >D04199A2_A6L1_1_BGH_rev2_F04_16_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNGGG GGNNGGNNNN NNACAGATGG CTGGCAACTA GAAGGCACAG
           61 TCGAGGCTGA TCAGCGAGCT CTAGGAATTC TTAGCTGGCC TCCACCTTTC TCTTCTTCTT
          121 GGGGCTGAAT TCGACGGAGT CCTCTGAGGG GATCTTGCCC GGCTGTTGAC CAACATAAAC
          181 AGTTGTACTC TTTGTTCGCT TGACGGGGGA TGGGCAAACG GGGGAGCCAG GGTCAGCTGG
          241 AGGGCTAGGG TCAGCTGGAG GGCTGGGCTC AGCTGGGGAA CTGGGCTCAG GCAGAGAACT
          301 GGGGCAGGAG TCCAATCCAG GCAGCCGTAA CCTCCGGCGC GCGAGCGGCG CGGGCTGGTC
          361 ACTCCCGGGT TCGGCTGGGG GCCGGGCGCG CTTGCGGCTG CTGCTCCGGG TGAACGCAGG
          421 AGCCACGAGG CCACCAGGGC TCGGGTCGCC TCCCAGCTGA GACAGGTCGA TCCGTGTCTC
          481 GTACAGGCCG GTGATGCTCT GGTGGATCAG GGTGGCGTCC AGCACCTCTT TGGTGCTGGT
          541 GTACCTCTTC CGGTCGATGG TGGTGTCAAA GTACTTGAAG GCGGCAGGGG CTCCCAGATT
          601 GGTCAGGGTA AACAGGTGGA TGATATTCTC GGCCTGCTCT CTGATGGGCT TATCCCGGTG
          661 CTTGTTGTAG GCGGACAGCA CTTTGTCCAG ATTAGCGTCG GCCAGGATCA CTCTCTTGGA
          721 NAACTCGCTG ATCTGCTCGA TGANCTCGTC CAGGTAGTGC TTGTGCTGTT CCACAAACAG
          781 CTGTTTCTGC TCATTATCCT CGGGGGANCC NNTCAGCTTC TCATAGTGGC TGGNCAGGTN
          841 CAGGAANNTC ACATATTTGG ANGGNANGGN CAGTTCGTTT CCNNTCTGCA GTTCGCCNGC
          901 ANANGNNANC ATTNNCTTCC NGNNNNTTTC CNNNNCNNAN NNNGNNNNAC NTNNNNAGCT
        961 NNNNNNNNNN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Mizuno-Iijima, S., Efficient production of large deletion and gene fragment knock-in mice mediated by genome editing with Cas9-mouse Cdt1 in mouse zygotes. Methods 191: 23-31 (2021). PMID 32334080.

        2022.02.09

        GNP_filter3_RDBDEP_html_220112.pl