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pLL3.7-FLAG-YAP1

Expression vector of FLAG-tagged human YAP1 with GFP marker.

Catalog number RDB14395
Resource name pLL3.7-FLAG-YAP1
Alternative name pLL3.7-K122-FLAG-YAP
Clone info. Expression vector of N-terminal FLAG-fused yes-associated protein 1 (YAP1) linked to the IRES and GFP under the CMV promoter.
Expression was confirmed by the depositor with immunoprecipitation and immunoblotting. Known difference to YAP1 (NM_001130145.2): C to T at 516nt (Ala 43 Val).
Vector backbone pLL3.7 K122 (plasmid)
Size of vector backbone 7.7 kb
Selectable markers Amp^r (E. coli)
Gene/insert name human YAP1 cDNA
Depositor Hata, Yutaka |
 
Other clones in our bank human YAP1 (NCBI Gene 10413) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nagashima, S. et al. Cancer Sci. 107 (6), 791-802, 2016) and an acknowledgment to the DEPOSITOR are requested.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks Remember that you will be working with samples containing infectious virus.
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14395 pLL3.7-FLAG-YAP1 Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Nagashima, S., Validation of chemical compound library screening for transcriptional co-activator with PDZ-binding motif inhibitors using GFP-fused transcriptional co-activator with PDZ-binding motif. Cancer Sci. 107 (6): 791-802 (2016). PMID 27009852.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Nagashima, S., Validation of chemical compound library screening for transcriptional co-activator with PDZ-binding motif inhibitors using GFP-fused transcriptional co-activator with PDZ-binding motif. Cancer Sci. 107 (6): 791-802 (2016). PMID 27009852.

2019.07.19

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