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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCoGFP-mam

Dual-color-emitting green fluorescent protein of sea cactus Cavernularia obesa.

Catalog number RDB14369
Resource name pCoGFP-mam
Clone info. Dual-color-emitting green fluorescent protein of sea cactus Cavernularia obesa. Mammalian codon optimized (Nucleotide sequence accession number: AB743882). pCoGFP-mam is not expression vector. When the fluorescent protein is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Cavernularia obesa GFP cDNA
Reference of insert sequence AB743882 (DDBJ accession number)
Depositor|Developer OLYMPUS CORPORATION, | Ogoh, Katsunori |
Other clones in our bank

External Database
Cavernularia obesa GFP

            Reference sequence
              
             
            Sequence (full) RDB14369zzt01.seq compiled by us

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Ogoh, K. Luminescence 28 (4): 582-591 (2013)). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって別紙に定める寄託者の指定する文献を引用する (Ogoh, K. Luminescence 28 (4): 582-591 (2013))。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び商業活動(*)を行う場合は、オリンパス株式会社と別途協議を行う。(*)商用活動とは,本件リソースが製品の一部に組み込まれる場合,本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。そのための基礎検討の使用に関しては,営利,非営利機関等の利用者制限は定めない。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB14369 pCoGFP-mam DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Ogoh, K., Dual-color-emitting green fluorescent protein from the sea cactus Cavernularia obesa and its use as a pH indicator for fluorescence microscopy. Luminescence 28 (4): 582-591 (2013). PMID 23468077.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Sensor and Visualization (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14369_A6Jcp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: reverse-2 (Pr0100)
            Region: insert
            Sequence file: RDB14369_A6Jcb.seq check
            >D03707B2_A6Jc_1_Reverse2_F02_17_ABI24.ab1
                1 NNNNNNNNNN NNGCNNGCTT GNNTGCCTGC AGGTCGANTC TAGAGGANCC GATGAGCATC
               61 CCCGAGAACA GCGGCCTGAC CGAAGAGATG CCCGCCCAGA TGAACCTGGA AGGGGTGGTC
              121 AACGGCCACG CCTTCTCCAT GGAAGGCATC GGCGGAGGCA ACATCCTGAC CGGCATCCAG
              181 AAACTGGACA TCAGAGTGAT CGAGGGCGAC CCCCTGCCCT TCAGCTTTGA CATCCTGAGC
              241 GTGGCCTTCC AGTACGGCAA CCGGACCTAC ACCAGCTACC CCGCCAAGAT CCCCGACTAC
              301 TTCGTGCAGA GCTTCCCCGA GGGCTTCACC TTCGAGCGGA CCCTGAGCTT TGAGGACGGC
              361 GCCATCGTGA AGGTGGAAAG CGACATCAGC ATCGAGGACG GCAAGTTCGT GGGCAAGATC
              421 AAGTACAACG GCGAGTCCTT CCCAGAGGAC GGCCCCGTGA TGAAGAAAGA AGTGACCAAG
              481 CTGGAACCCA GCAGCGAGTC TATGTACGTG TGCGACGACA CCCTCGTGGG CGAGGTGGTG
              541 CTGAGCTACA AGACCCAGAG CACCCACTAC ACCTGTCACA TGAAGACCAT CTACCGGTCC
              601 AAGAAACCCG TGGAAAACCT GCCCAAGTTC CACTACGTGC ACCACCGGCT CGAAAAGAAA
              661 AAGGTCGAAG AGGGCAACTA CTACGAGCAG CACGAGACAG CCATTGCCAA GCCCTGAGAA
              721 TTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGNAAAC CCTGGCGTTA CCCAACTTAA
              781 TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT ANCGAANANG CCCGCACCGA
              841 TCGCCCTTCC CNACAGTTGC GCAGCCNGAA TGGNGAATGG CGCCNGATGC GGNATTTTCN
              901 CCTTACGCAT CTGNNCGGNA TTTCACACCN NNNANGNNGN NNTNNCANNN NNATCNGNNN
            961 NNNNNNN
            //
            Primer: pUC-CAPbs_up_F (Pr0536)
            Region: insert 5'
            Sequence file: RDB14369_A6Jcc.seq check
            >14369_14369_B2KoF_pUC-CAPbs_up_F_C02_03_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NANNGCAATT AATGTGAGTT AGCTCACTCA
               61 TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG
              121 CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC TTGCATGCCT
              181 GCAGGTCGAC TCTAGAGGAT CCGATGAGCA TCCCCGAGAA CAGCGGCCTG ACCGAAGAGA
              241 TGCCCGCCCA GATGAACCTG GAAGGGGTGG TCAACGGCCA CGCCTTCTCC ATGGAAGGCA
              301 TCGGCGGAGG CAACATCCTG ACCGGCATCC AGAAACTGGA CATCAGAGTG ATCGAGGGCG
              361 ACCCCCTGCC CTTCAGCTTT GACATCCTGA GCGTGGCCTT CCAGTACGGC AACCGGACCT
              421 ACACCAGCTA CCCCGCCAAG ATCCCCGACT ACTTCGTGCA GAGCTTCCCC GAGGGCTTCA
              481 CCTTCGAGCG GACCCTGAGC TTTGAGGACG GCGCCATCGT GAAGGTGGAA AGCGACATCA
              541 GCATCGAGGA CGGCAAGTTC GTGGGCAAGA TCAAGTACAA CGGCGAGTCC TTCCCAGAGG
              601 ACGGCCCCGT GATGAAGAAA GAAGTGACCA AGCTGGAACC CAGCAGCGAG TCTATGTACG
              661 TGTGCGACGA CACCCTCGTG GGCGAGGTGG TGCTGAGCTA CNAGACCCAG AGCACCCACT
              721 ACACCTGTCA CATGAAGACC ATCTACCGGT CCAAGAAACC CGTGGAAAAC CTGCCCAAGT
              781 TCCACTACGT GCACCACCGG CTCGAAAAGA AAAAAGGNCG AAGANGGCAN CTNCTACGAG
              841 CAGCACGAGA CAGCCNTTGC NNAAGCCCTG AGAATTCACT GGCCGTCGTT TTACNACGNC
              901 GTGACTGGGN AAAACCCTGN CGTTNNCCAN CTTAATCNNC TTGCAGCNCA TNCCCNNTTC
              961 NNNNNCNNGN GTAATANCGA ANNAGGNCCN CNNCGNTNGC CCTNNCCNNC NNTNGNNCNN
            1021 NCNTGNNNNN NNNANNNNNN CNGNTGNNNN ATTTTNNNCC NTNANNNNNN NNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Ogoh, K., Dual-color-emitting green fluorescent protein from the sea cactus Cavernularia obesa and its use as a pH indicator for fluorescence microscopy. Luminescence 28 (4): 582-591 (2013). PMID 23468077.

            2022.11.29

            GNP_filter3_RDBDEP_html_221125.pl