Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCoGFP-mam (#RDB14369)

Dual-color-emitting green fluorescent protein of sea cactus Cavernularia obesa.

Clone info. Dual-color-emitting green fluorescent protein of sea cactus Cavernularia obesa. Mammalian codon optimized (Nucleotide sequence accession number: AB743882). pCoGFP-mam is not expression vector. When the fluorescent protein is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Cavernularia obesa GFP cDNA
Reference of insert sequence AB743882 (DDBJ accession number)
Depositor|Developer OLYMPUS CORPORATION, | Ogoh, Katsunori |
 
Sequence (full) RDB14369zzt01.seq compiled by us

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Ogoh, K. Luminescence 28 (4): 582-591 (2013)). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって別紙に定める寄託者の指定する文献を引用する (Ogoh, K. Luminescence 28 (4): 582-591 (2013))。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び商業活動(*)を行う場合は、オリンパス株式会社と別途協議を行う。(*)商用活動とは，本件リソースが製品の一部に組み込まれる場合，本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。そのための基礎検討の使用に関しては，営利，非営利機関等の利用者制限は定めない。

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RDB14369 pCoGFP-mam DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCoGFP-mam was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14369).

Reference section:

Ogoh, K., Kinebuchi, T., Murai, M., Takahashi, T., Ohmiya, Y., Suzuki, H., Dual-color-emitting green fluorescent protein from the sea cactus Cavernularia obesa and its use as a pH indicator for fluorescence microscopy. Luminescence 28 (4): 582-591 (2013). PMID 23468077. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14369_A6Jcp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: reverse-2 (Pr0100)
Region: insert
Sequence file: RDB14369_A6Jcb.seq check
>D03707B2_A6Jc_1_Reverse2_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNGCNNGCTT GNNTGCCTGC AGGTCGANTC TAGAGGANCC GATGAGCATC
   61 CCCGAGAACA GCGGCCTGAC CGAAGAGATG CCCGCCCAGA TGAACCTGGA AGGGGTGGTC
  121 AACGGCCACG CCTTCTCCAT GGAAGGCATC GGCGGAGGCA ACATCCTGAC CGGCATCCAG
  181 AAACTGGACA TCAGAGTGAT CGAGGGCGAC CCCCTGCCCT TCAGCTTTGA CATCCTGAGC
  241 GTGGCCTTCC AGTACGGCAA CCGGACCTAC ACCAGCTACC CCGCCAAGAT CCCCGACTAC
  301 TTCGTGCAGA GCTTCCCCGA GGGCTTCACC TTCGAGCGGA CCCTGAGCTT TGAGGACGGC
  361 GCCATCGTGA AGGTGGAAAG CGACATCAGC ATCGAGGACG GCAAGTTCGT GGGCAAGATC
  421 AAGTACAACG GCGAGTCCTT CCCAGAGGAC GGCCCCGTGA TGAAGAAAGA AGTGACCAAG
  481 CTGGAACCCA GCAGCGAGTC TATGTACGTG TGCGACGACA CCCTCGTGGG CGAGGTGGTG
  541 CTGAGCTACA AGACCCAGAG CACCCACTAC ACCTGTCACA TGAAGACCAT CTACCGGTCC
  601 AAGAAACCCG TGGAAAACCT GCCCAAGTTC CACTACGTGC ACCACCGGCT CGAAAAGAAA
  661 AAGGTCGAAG AGGGCAACTA CTACGAGCAG CACGAGACAG CCATTGCCAA GCCCTGAGAA
  721 TTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGNAAAC CCTGGCGTTA CCCAACTTAA
  781 TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT ANCGAANANG CCCGCACCGA
  841 TCGCCCTTCC CNACAGTTGC GCAGCCNGAA TGGNGAATGG CGCCNGATGC GGNATTTTCN
  901 CCTTACGCAT CTGNNCGGNA TTTCACACCN NNNANGNNGN NNTNNCANNN NNATCNGNNN
961 NNNNNNN
//
Primer: pUC-CAPbs_up_F (Pr0536)
Region: insert 5'
Sequence file: RDB14369_A6Jcc.seq check
>14369_14369_B2KoF_pUC-CAPbs_up_F_C02_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NANNGCAATT AATGTGAGTT AGCTCACTCA
   61 TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG
  121 CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC TTGCATGCCT
  181 GCAGGTCGAC TCTAGAGGAT CCGATGAGCA TCCCCGAGAA CAGCGGCCTG ACCGAAGAGA
  241 TGCCCGCCCA GATGAACCTG GAAGGGGTGG TCAACGGCCA CGCCTTCTCC ATGGAAGGCA
  301 TCGGCGGAGG CAACATCCTG ACCGGCATCC AGAAACTGGA CATCAGAGTG ATCGAGGGCG
  361 ACCCCCTGCC CTTCAGCTTT GACATCCTGA GCGTGGCCTT CCAGTACGGC AACCGGACCT
  421 ACACCAGCTA CCCCGCCAAG ATCCCCGACT ACTTCGTGCA GAGCTTCCCC GAGGGCTTCA
  481 CCTTCGAGCG GACCCTGAGC TTTGAGGACG GCGCCATCGT GAAGGTGGAA AGCGACATCA
  541 GCATCGAGGA CGGCAAGTTC GTGGGCAAGA TCAAGTACAA CGGCGAGTCC TTCCCAGAGG
  601 ACGGCCCCGT GATGAAGAAA GAAGTGACCA AGCTGGAACC CAGCAGCGAG TCTATGTACG
  661 TGTGCGACGA CACCCTCGTG GGCGAGGTGG TGCTGAGCTA CNAGACCCAG AGCACCCACT
  721 ACACCTGTCA CATGAAGACC ATCTACCGGT CCAAGAAACC CGTGGAAAAC CTGCCCAAGT
  781 TCCACTACGT GCACCACCGG CTCGAAAAGA AAAAAGGNCG AAGANGGCAN CTNCTACGAG
  841 CAGCACGAGA CAGCCNTTGC NNAAGCCCTG AGAATTCACT GGCCGTCGTT TTACNACGNC
  901 GTGACTGGGN AAAACCCTGN CGTTNNCCAN CTTAATCNNC TTGCAGCNCA TNCCCNNTTC
  961 NNNNNCNNGN GTAATANCGA ANNAGGNCCN CNNCGNTNGC CCTNNCCNNC NNTNGNNCNN
1021 NCNTGNNNNN NNNANNNNNN CNGNTGNNNN ATTTTNNNCC NTNANNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ogoh, K., Dual-color-emitting green fluorescent protein from the sea cactus Cavernularia obesa and its use as a pH indicator for fluorescence microscopy. Luminescence 28 (4): 582-591 (2013). PMID 23468077. [link to RRC of NBRP]