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pCoGFP-mam

Dual-color-emitting green fluorescent protein of sea cactus Cavernularia obesa.

Catalog number RDB14369
Resource name pCoGFP-mam
Clone info. Dual-color-emitting green fluorescent protein of sea cactus Cavernularia obesa. Mammalian codon optimized (Nucleotide sequence accession number: AB743882). pCoGFP-mam is not expression vector. When the fluorescent protein is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Cavernularia obesa GFP cDNA
Reference of nucleotide sequence AB743882 (DDBJ accession number)
Depositor OLYMPUS CORPORATION, | Ogoh, Katsunori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Ogoh, K. Luminescence 28 (4): 582-591 (2013)). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14369 pCoGFP-mam DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Ogoh, K., Dual-color-emitting green fluorescent protein from the sea cactus Cavernularia obesa and its use as a pH indicator for fluorescence microscopy. Luminescence 28 (4): 582-591 (2013). PMID 23468077.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Sensor and Visualization (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14369_A6Jcp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Reverse2
Sequence file: RDB14369_A6Jca.seq check
>D03707B2_A6Jc_1_Reverse2_F02_17_ABI24.ab1
    1 AATCATCATT ACGCCAGCTT GCATGCCTGC AGGTCGACTC TAGAGGATCC GATGAGCATC
   61 CCCGAGAACA GCGGCCTGAC CGAAGAGATG CCCGCCCAGA TGAACCTGGA AGGGGTGGTC
  121 AACGGCCACG CCTTCTCCAT GGAAGGCATC GGCGGAGGCA ACATCCTGAC CGGCATCCAG
  181 AAACTGGACA TCAGAGTGAT CGAGGGCGAC CCCCTGCCCT TCAGCTTTGA CATCCTGAGC
  241 GTGGCCTTCC AGTACGGCAA CCGGACCTAC ACCAGCTACC CCGCCAAGAT CCCCGACTAC
  301 TTCGTGCAGA GCTTCCCCGA GGGCTTCACC TTCGAGCGGA CCCTGAGCTT TGAGGACGGC
  361 GCCATCGTGA AGGTGGAAAG CGACATCAGC ATCGAGGACG GCAAGTTCGT GGGCAAGATC
  421 AAGTACAACG GCGAGTCCTT CCCAGAGGAC GGCCCCGTGA TGAAGAAAGA AGTGACCAAG
  481 CTGGAACCCA GCAGCGAGTC TATGTACGTG TGCGACGACA CCCTCGTGGG CGAGGTGGTG
  541 CTGAGCTACA AGACCCAGAG CACCCACTAC ACCTGTCACA TGAAGACCAT CTACCGGTCC
  601 AAGAAACCCG TGGAAAACCT GCCCAAGTTC CACTACGTGC ACCACCGGCT CGAAAAGAAA
  661 AAGGTCGAAG AGGGCAACTA CTACGAGCAG CACGAGACAG CCATTGCCAA GCCCTGAGAA
  721 TTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA
  781 TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA
  841 TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG CGCCTGATGC GGTATTTTCT
  901 CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATGGTGC ACTCTCAGTA CAATCTGCTC
  961 TGATGCCGCA TAGTTTAAGC CAGCCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA
 1021 CGGGCTTGTC TGCTCCCGGC ATCCGGCTAC AGACAGCTGT GACGTCTCGG CAGCCTGCCA
1081 TGTTTCAGAG GATTTTTCCA CCCGATCT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ogoh, K., Dual-color-emitting green fluorescent protein from the sea cactus Cavernularia obesa and its use as a pH indicator for fluorescence microscopy. Luminescence 28 (4): 582-591 (2013). PMID 23468077.

2019.12.18

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