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pCoGFP-Th2co

Thermostable green fluorescent protein of sea cactus Cavernularia obesa.

Catalog number RDB14367
Resource name pCoGFP-Th2co
Clone info. Thermostable green fluorescent protein of sea cactus Cavernularia obesa. Mutant 2 codon optimized. pCoGFP-Th2co is not expression vector. When the fluorescent protein is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Cavernularia obesa GFP cDNA
Depositor|Developer OLYMPUS CORPORATION, | Ogoh, Katsunori |
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External Database
Cavernularia obesa GFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Murai M, Ogoh K, Kinebuchi T, Suzuki H. Thermostable fluorescent protein and its utility. Japan patent publication 2014-60947). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって別紙に定める寄託者の指定する文献を引用する (熱安定性を有する蛍光タンパク質およびそれを利用する方法 特開2014-60947)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び商業活動(*)を行う場合は、オリンパス株式会社と別途協議を行う。(*)商用活動とは,本件リソースが製品の一部に組み込まれる場合,本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。そのための基礎検討の使用に関しては,営利,非営利機関等の利用者制限は定めない。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB14367 pCoGFP-Th2co DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Murai, M., Thermostable fluorescent protein and its utility. Japan Patent Abstracts 2014-60947.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14367_A6Jcp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: Reverse-2
            Region: insert
            Sequence file: RDB14367_A6Jca.seq check
            >D03707A9_A6Jc_1_Reverse2_B02_05_ABI24.ab1
                1 CGGAAAGCAT TACGCCAGCT TGCATGCCTG CAGGTCGACT CTAGAGGATC CGATGAGCAT
               61 CCCCGAGAAC AGCGGCCTGA CCGAAGAGAT GCCCGCCCAG ATGAACCTGG AAGGGGTGGT
              121 CAACGGCCAC GCCTTCTCCA TGGAAGGCAT CGGCGGAGGC AACATCCTGA CCGGCATCCA
              181 GAAACTGGAC ATCAGAGTGA CCGAGGGCGA CCCCCTGCCC TTCAGCTTTG ACATCCTGAG
              241 CGTGGCCTTC CAGTACGGCA ACCGGACCTA CACCAGCTAC CCCGCCAAGA TCCCCGACTA
              301 CTTCGTGCAG AGCTTCCCCG AGGGCTTCAC CTTCGAGCGG ACCCTGAGCT TTGAGGACGG
              361 CGCCATCGTG AAGGTGGAAA GCGACATCAG CATCGAGGAC GGCAAGTTCG TGGGCAAGAT
              421 CAAGTACAAC GGCGAGGGCT TCCCAGAGGA CGGCCCCGTG ATGAAGAAAG AAGTGACCAA
              481 GCTGGAACCC AGCAGCGAGT CTATGTACGT GAGCGACGGC ACCCTCGTGG GCGAGGTGGT
              541 GCTGAGCTAC AAGACCCAGA GCACCCACTA CACCTGTCAC ATGAAGACCA TCTACCGGTC
              601 CAAGAAACCC GTGGAAAACC TGCCCAAGTT CCACTACGTG CACCACCGGC TCGAAAAGAA
              661 AATCGTCGAA GAGGGCTACT ACTACGAGCA GCACGAGACA GCCATTGCCA AGCCCTGAGA
              721 ATTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA
              781 ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG
              841 ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GCGCCTGATG CGGTATTTTC
              901 TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATGGTG CACTCTCAGT ACAATCTGCT
              961 CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCCAA CACCCGCTGA CGCGGCCCTG
             1021 ACGGGCTTTG TCTGCTCCGG CCATCCGGCT TTACAGACAG CTGTGACGTC TCCGGCAAGC
            1081 TGCATGTGTT CAGGAGGATT TTTCAACG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Murai, M., Thermostable fluorescent protein and its utility. Japan Patent Abstracts 2014-60947.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl