Thermostable green fluorescent protein of sea cactus Cavernularia obesa. Mutant 2.
Clone info. | Thermostable green fluorescent protein of sea cactus Cavernularia obesa. Mutant 2. pCoGFP-Th2wt is not expression vector. When the fluorescent protein is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own. |
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Vector backbone | pUC19 (Plasmid) |
Size of vector backbone | 2.7 kb |
Selectable markers | Amp^r |
Gene/insert name | Cavernularia obesa GFP cDNA |
Depositor|Developer | OLYMPUS CORPORATION, | Ogoh, Katsunori | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Murai M, Ogoh K, Kinebuchi T, Suzuki H. Thermostable fluorescent protein and its utility. Japan patent publication 2014-60947). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって別紙に定める寄託者の指定する文献を引用する (熱安定性を有する蛍光タンパク質およびそれを利用する方法 特開2014-60947)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び商業活動(*)を行う場合は、オリンパス株式会社と別途協議を行う。(*)商用活動とは,本件リソースが製品の一部に組み込まれる場合,本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。そのための基礎検討の使用に関しては,営利,非営利機関等の利用者制限は定めない。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB14366 | pCoGFP-Th2wt | DNA solution |
Materials & Methods section:
The pCoGFP-Th2wt was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14366). |
Reference section:
Murai, M., Ogoh, K., Kinebuchi, T., Suzuki, H., Thermostable fluorescent protein and its utility. Japan Patent Abstracts 2014-60947. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB14366_A6Jcp1-2.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: AFR (Pr0324) Region: insert Sequence file: RDB14366_A6Jca.seq |
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>D03707A8_A6Jc_2_AFR_D12_12_ABI24.ab1 1 CTCACGCTAT GACCATGATT ACGCCAAGCT TTCATGGTTT AGCTATGGCC GTCTCATGTT 61 GCTCGTAGTA GTAGCCTTCC TCGACTATTT TCTTTTCGAG GCGATGATGA ACATAATGGA 121 ACTTTGGCAG GTTTTCTACT GGCTTTTTGG ACCTGTAGAT TGTTTTCATA TGGCAAGTGT 181 AATGAGTCGA CTGGGTCTTG TAAGACAACA CAACTTCTCC CACCAAAGTG CCGTCCGATA 241 CATACATTGA CTCACTGGAC GGTTCTAGTT TGGTAACTTC CTTTTTCATC ACCGGTCCAT 301 CCTCTGGAAA ACCTTCGCCA TTATATTTAA TCTTGCCAAC AAATTTGCCA TCTTCAATAC 361 TAATATCCGA TTCGACCTTT ACGATTGCAC CATCTTCAAA ACTAAGAGTC CTTTCAAAGG 421 TGAACCCTTC TGGGAATGAT TGAACGAAAT AATCGGGGAT CTTCGCAGGA TAACTTGTGT 481 AAGTTCTGTT GCCATATTGA AACGCAACTG AGAGAATGTC AAAGGAAAAC GGAAGAGGAT 541 CTCCCTCGGT GACACGGATA TCCAGCTTTT GAATTCCAGT TAGTATGTTC CCTCCACCAA 601 TTCCTTCCAT AGAGAAGGCA TGACCATTTA CCACTCCTTC TAAATTCATT TGAGCAGGCA 661 TCTCTTCTGT TAAGCCCGAA TTCTCTGGAA TACTCATGGA TCCCCGGGTA CCGAGCTCGA 721 ATTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA 781 ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG 841 ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GCGCCTGATG CGGTATTTTC 901 TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATGGTG CACTCTCAGT ACAATCTGCT 961 CTGATGCCGC ATAGTTTAAG CCAGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA 1021 CGGGCTTGTC TGCTCCCGGG CATCCGCTTA CAGACAGCTG TGACGTCTCC GGAGCTGCAT 1081 GTGTCAGAGA TTTCACGTCA TCACCGAACG CGCGGACGAA CGACTTCGTG AATACGCTAT 1141 TTTTTAATAG GATTAATTGT TCCATGGATA AT // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Murai, M., Thermostable fluorescent protein and its utility. Japan Patent Abstracts 2014-60947. [link to RRC of NBRP] |
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