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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMALuci2Luc

Green-shifted luciferase of Malaysian Luciola firefly with higher luminous intensity.

Catalog number RDB14363
Resource name pMALuci2Luc
Clone info. Green-shifted luciferase of Malaysian Luciola firefly with higher luminous intensity. A maximum luminescent wavelength falls 557 nm at pH8 and within a range of 555 to 560 nm over the pH range of 5.5 to 8.0 (Fig. 1 of patent application publication US 2015/0291938 A1). pMALuci2Luc is not expression vector. When the luciferase is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Comment The luciferase gene originated from Malaysian firefly was developed together with Perak State Development Corporation and Nimura Genetic Solutions in conformity with the Convention on Biological Diversity.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Luciola sp2 Luciferase cDNA
Depositor|Developer OLYMPUS CORPORATION, | Ogoh, Katsunori |
Other clones in our bank

External Database
Luciola sp2 Luciferase

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Akiyoshi R, Ogoh K, Suzuki H. 2015. FIREFLY LUCIFERASE, Patent publication US2015/0291938 A1). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって別紙に定める寄託者の指定する文献を引用する (Akiyoshi R, Ogoh K, Suzuki H. 2015. FIREFLY LUCIFERASE, Patent publication US2015/0291938 A1)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び商業活動(*)を行う場合は、オリンパス株式会社と別途協議を行う。(*)商用活動とは,本件リソースが製品の一部に組み込まれる場合,本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。そのための基礎検討の使用に関しては,営利,非営利機関等の利用者制限は定めない。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB14363 pMALuci2Luc DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Akiyoshi, R., FIREFLY LUCIFERASE. Patent publication US2015/0291938 A1.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Higher intensity luciferase (English text)
          Featured content Higher intensity luciferase (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14363_A6Jcp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: reverse-2
          Region: insert 5'
          Sequence file: RDB14363_A6Jcc.seq check
          >D03707A5_A6Jc_1_Reverse2_B01_04_ABI24.ab1
              1 NNNNNNNNNN NNNCNNGCTT GNATGCCTGC NGGTCGANTC TAGAGGNNCC GATGAACAAG
             61 AACATCATCT ACGGCCCTCC CCCCGTGTAC CCCCTGGATG ATGGCACAGG CGGCGAGCAG
            121 CTGTACAAGT GCATCCTGAG ATACGCCAAG ATCCCCGAGT GCGTGGCCCT GACCAGCGCC
            181 CACACCAAAG AGAGCATCCT GTACGAGGAA CTGCTGCAGC TGACCTGCAA GCTGGCCCAG
            241 AGCCTGAAGA GATGCGGCAT CACCCGGAAC AGCACAATCG CCGTGTGCAG CGAGAACAAC
            301 CTGCAGTACT TCATCCCCAT CATTGCCGGC CTGTACATCG GAGCCGCCAC AGCCGCCGTG
            361 AACAACCGGT ACAACGAGAG AGAGCTGACC GACATCCTGA ACCTGAGCAA GCCCGACATC
            421 ATCTTTTGCT CCAAAGAGAC ACTGCCCAAG ATCTGCCAGG TCAAGAAGAA GCTGAACTAC
            481 ATCAAAGAAA TCATCGTGCT GGACAGCAAG CACGACAGCG AGCTGGCTCA GTGTCTGGAC
            541 AACTTCATCA GCCACAACTG CAACAAGGAC TTCGACGCCT ACCAGTTCAA GCCCAGCAGC
            601 TTCAACCGGA ACGAACAGGT CGGCCTGATC CTGAACAGCA GCGGCAGCAC CGGCCTGCCC
            661 AAGGGCGTGA TGCTGACCCA CAAGAACCTG GTGGTGCGCT TCAGCCACTG CAAGGACCCC
            721 GTGTTCGGCA ACATCATCAG CCCCGGCACC GCCATCCTGA CCGTGATCCC TTTCCACCAC
            781 GGCTTCNGCA TGTTCACCAC CCTGGGCTAN TTCACCTGNG GCTTCCGGAT CGTGCTGATG
            841 CACACCTTCN ACGANAAGCT GTNCCTGCAN GCCCNNGAAG ANTACAAGGN GGAAAGCACC
          901 CNGCNNGNGC CNANCCNGAT NANCNTCNTC GNNNNNNNCG NCNNNNNNNN NN
          //
          Primer: pUC-M13_-100
          Region: insert 3'
          Sequence file: RDB14363_A6Jcd.seq check
          >D03707A5_A6Jc_1_pUC_M13-100_A01_01_ABI24.ab1
              1 NNNNNNNNNN NNNNNNTNGG NNNNGCCNGG GNTTTCCCNG TCNCGACGTT GTAAAACGAC
             61 GGCCAGTGAA TTCTCACAGC TTGCAGTTGG GCTTCTGCAG GATCTGCTTC AGGGCGGTGG
            121 TGTCGATCTT GCCGGTGGAG CCCTTAGGGA TCTCGTCCAG GAACCGCACG CCGCCTCTCA
            181 GCCGCTTCAC GCTGGACACC TGGCTGGACA CGTAGTTGAT CACGTTTTCC TCGGTCAGGT
            241 GTCTGCCGGG TTTCACCACC ACGCAGGCGG CAGGCAGCTC GCCGGCATTA TCGTCGGGGA
            301 TGCCGGTCAC GCCGGCATCC ATGATGCTGG GGTGTGTCAG CAGGATGCCT TCCAGCTCGG
            361 CAGGGGCCAC CTGGTAGCCC TTGTACTTGA TGATGGACTT CAGCCGGTCC ACGATGAAGA
            421 AGTGCTTGTC GTCGTTGTAG TAGCCCAGAT CGCCGCTGTG CAGCCAGCCG TCGTTGTCGA
            481 TGATGGCGTT GGTGGCCTCG ATGTTGTTGC AGTAGCCCTT CATGATCATG TCGCCCTTGA
            541 AGCACAGCTC GCCCACTTCA TTAGGGCCCA GGGCCTTGCC GGTCCGGTGG TCGATGATCT
            601 TGGCGGCGAA GAATGGCACC ACCTTGCCGA TGCNNNNGGG CACTGTCTCG CTCTCGGGGG
            661 TGATCAGCAC GGCGCTGGTT GTCTCGGTCA GGCCGTAGCC CTGCCGGATG GCGTTCAGCT
            721 TGAACCGTCT GGCCACGGCC TCGCCGATTT CTTTGCTCAG GGGGGCTCCG CCGCTGGCGA
            781 TCTCTTTCAN GTANGGCAGG TTGTACTTGT CCACCANGGC GCTCTTGGCG AANAANGTCA
            841 TCANGGNANG CACCAGCANG GNGCTTTCCA CCTNNNNATC TTNNAGGGNC TGCANGANCA
          901 GCTTCNCGTA NAANNNNGNG NNTCANCACN NNNCNGNNNN NNNNNN
          //
          Primer: Luc2-M_F
          Region: insert mid
          Sequence file: RDB14363_A6Jce.seq check
          >14363_14363_B1IoF_Luc2-M_F_E02_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNTTCN NNNNCNTCAT CAGCCCCGGC ACCGCCATCC
             61 TGACCGTGAT CCCTTTCCAC CACGGCTTCG GCATGTTCAC CACCCTGGGC TACTTCACCT
            121 GTGGCTTCCG GATCGTGCTG ATGCACACCT TCTACGAGAA GCTGTTCCTG CAGGCCCTGG
            181 AAGATTACAA GGTGGAAAGC ACCCTGCTGG TGCCTACCCT GATGACCTTC TTCGCCAAGA
            241 GCGCCCTGGT GGACAAGTAC AACCTGCCCT ACCTGAAAGA GATCGCCAGC GGCGGAGCCC
            301 CCCTGAGCAA AGAAATCGGC GAGGCCGTGG CCAGACGGTT CAAGCTGAAC GCCATCCGGC
            361 AGGGCTACGG CCTGACCGAG ACAACCAGCG CCGTGCTGAT CACCCCCGAG AGCGAGACAG
            421 TGCCCGGCAG CATCGGCAAG GTGGTGCCAT TCTTCGCCGC CAAGATCATC GACCACCGGA
            481 CCGGCAAGGC CCTGGGCCCT AATGAAGTGG GCGAGCTGTG CTTCNNNGGC GACATGATCA
            541 TGANGGGCTA CTGCAACNNC ATCGAGGCCA CCNACGCCAT CATCGACAAC GACGGCTGGC
            601 TGCACAGCNG CGATCTGGGC TACTACNACG ANNACNANCA CTTCNTCATC NTGGANCGGC
            661 TGANNTCNNT CATCNAGTAC NNGGGNTANN NNGTGGNCCC CNNNCNNANC NNNNNNNANN
          721 NNGNNNNNNN NCNNNCNNNN NNNNNN
          //
          Primer: Luc2-M_R
          Region: insert mid
          Sequence file: RDB14363_A6Jcf.seq check
          >14363_14363_B1IoF_Luc2-M_R_F02_06_ABI08.ab1
              1 NNNNNNNNNN NNGNNNNNNN NCTTGGCGGC GNANANNGGC ACCACCTTGC CGATGCTGCC
             61 GGGCACTGTC TCGCTCTCGG GGGTGATCAG CACGGCGCTG GTTGTCTCGG TCAGGCCGTA
            121 GCCCTGCCGG ATGGCGTTCA GCTTGAACCG TCTGGCCACG GCCTCGCCGA TTTCTTTGCT
            181 CAGGGGGGCT CCGCCGCTGG CGATCTCTTT CAGGTAGGGC AGGTTGTACT TGTCCACCAG
            241 GGCGCTCTTG GCGAAGAAGG TCATCAGGGT AGGCACCAGC AGGGTGCTTT CCACCTTGTA
            301 ATCTTCCAGG GCCTGCAGGA ACAGCTTCTC GTAGAAGGTG TGCATCAGCA CGATCCGGAA
            361 GCCACAGGTG AAGTAGCCCA GGGTGGTGAA CATGCCGAAG CCGTGGTGGA AAGGGATCAC
            421 GGTCAGGATG GCGGTGCCGG GGCTGATGAT GTTGCCGAAC ACGGGGTCCT TGCAGTGGCT
            481 GAAGCGCACC ACCAGGTTCT TGTGGGTCAG CATCACGCCC TTGGGCAGGC CGGTGCTGCC
            541 GCTGCTGTTC AGGATCAGGC CGACCTGTTC GTTCCGGTTG AAGCTGCTGG GCTTGAACTG
            601 GTAGGCGTCG AAGTCCTTGT TGCAGTTGNG GCTNANGAAG TTGTCCAGAC ACTGAGCNNG
            661 CTCGCTGNCG TGNTTGCTGT CCANCACGAN GANTTCNTTN NNGNANNNNN NCNTNNNNNN
          721 GANCNNNNNN NNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akiyoshi, R., FIREFLY LUCIFERASE. Patent publication US2015/0291938 A1.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl