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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMALuci2Luc (#RDB14363)

Green-shifted luciferase of Malaysian Luciola firefly with higher luminous intensity.

Clone info. Green-shifted luciferase of Malaysian Luciola firefly with higher luminous intensity. A maximum luminescent wavelength falls 557 nm at pH8 and within a range of 555 to 560 nm over the pH range of 5.5 to 8.0 (Fig. 1 of patent application publication US 2015/0291938 A1). pMALuci2Luc is not expression vector. When the luciferase is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Comment The luciferase gene originated from Malaysian firefly was developed together with Perak State Development Corporation and Nimura Genetic Solutions in conformity with the Convention on Biological Diversity.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Luciola sp2 Luciferase cDNA
Depositor|Developer OLYMPUS CORPORATION, | Ogoh, Katsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Akiyoshi R, Ogoh K, Suzuki H. 2015. FIREFLY LUCIFERASE, Patent publication US2015/0291938 A1). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって別紙に定める寄託者の指定する文献を引用する (Akiyoshi R, Ogoh K, Suzuki H. 2015. FIREFLY LUCIFERASE, Patent publication US2015/0291938 A1)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び商業活動(*)を行う場合は、オリンパス株式会社と別途協議を行う。(*)商用活動とは，本件リソースが製品の一部に組み込まれる場合，本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。そのための基礎検討の使用に関しては，営利，非営利機関等の利用者制限は定めない。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14363 pMALuci2Luc DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMALuci2Luc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14363).

Reference section:

Ogoh, K., Akiyoshi, R., Suzuki, H., Cloning and mutagenetic modification of the firefly luciferase gene and its use for bioluminescence microscopy of engrailed expression during Drosophila metamorphosis. Biochem. Biophys. Rep. 23: 100771 (2020). PMID 32490216. [link to RRC of NBRP]
Akiyoshi, R., Ogoh, K., Suzuki, H., FIREFLY LUCIFERASE. Patent publication US2015/0291938 A1. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14363_A6Jcp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: reverse-2 (Pr0100)
Region: insert 5'
Sequence file: RDB14363_A6Jcc.seq check
>D03707A5_A6Jc_1_Reverse2_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNCNNGCTT GNATGCCTGC NGGTCGANTC TAGAGGNNCC GATGAACAAG
   61 AACATCATCT ACGGCCCTCC CCCCGTGTAC CCCCTGGATG ATGGCACAGG CGGCGAGCAG
  121 CTGTACAAGT GCATCCTGAG ATACGCCAAG ATCCCCGAGT GCGTGGCCCT GACCAGCGCC
  181 CACACCAAAG AGAGCATCCT GTACGAGGAA CTGCTGCAGC TGACCTGCAA GCTGGCCCAG
  241 AGCCTGAAGA GATGCGGCAT CACCCGGAAC AGCACAATCG CCGTGTGCAG CGAGAACAAC
  301 CTGCAGTACT TCATCCCCAT CATTGCCGGC CTGTACATCG GAGCCGCCAC AGCCGCCGTG
  361 AACAACCGGT ACAACGAGAG AGAGCTGACC GACATCCTGA ACCTGAGCAA GCCCGACATC
  421 ATCTTTTGCT CCAAAGAGAC ACTGCCCAAG ATCTGCCAGG TCAAGAAGAA GCTGAACTAC
  481 ATCAAAGAAA TCATCGTGCT GGACAGCAAG CACGACAGCG AGCTGGCTCA GTGTCTGGAC
  541 AACTTCATCA GCCACAACTG CAACAAGGAC TTCGACGCCT ACCAGTTCAA GCCCAGCAGC
  601 TTCAACCGGA ACGAACAGGT CGGCCTGATC CTGAACAGCA GCGGCAGCAC CGGCCTGCCC
  661 AAGGGCGTGA TGCTGACCCA CAAGAACCTG GTGGTGCGCT TCAGCCACTG CAAGGACCCC
  721 GTGTTCGGCA ACATCATCAG CCCCGGCACC GCCATCCTGA CCGTGATCCC TTTCCACCAC
  781 GGCTTCNGCA TGTTCACCAC CCTGGGCTAN TTCACCTGNG GCTTCCGGAT CGTGCTGATG
  841 CACACCTTCN ACGANAAGCT GTNCCTGCAN GCCCNNGAAG ANTACAAGGN GGAAAGCACC
901 CNGCNNGNGC CNANCCNGAT NANCNTCNTC GNNNNNNNCG NCNNNNNNNN NN
//
Primer: pUC-M13_-100 (Pr0310)
Region: insert 3'
Sequence file: RDB14363_A6Jcd.seq check
>D03707A5_A6Jc_1_pUC_M13-100_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTNGG NNNNGCCNGG GNTTTCCCNG TCNCGACGTT GTAAAACGAC
   61 GGCCAGTGAA TTCTCACAGC TTGCAGTTGG GCTTCTGCAG GATCTGCTTC AGGGCGGTGG
  121 TGTCGATCTT GCCGGTGGAG CCCTTAGGGA TCTCGTCCAG GAACCGCACG CCGCCTCTCA
  181 GCCGCTTCAC GCTGGACACC TGGCTGGACA CGTAGTTGAT CACGTTTTCC TCGGTCAGGT
  241 GTCTGCCGGG TTTCACCACC ACGCAGGCGG CAGGCAGCTC GCCGGCATTA TCGTCGGGGA
  301 TGCCGGTCAC GCCGGCATCC ATGATGCTGG GGTGTGTCAG CAGGATGCCT TCCAGCTCGG
  361 CAGGGGCCAC CTGGTAGCCC TTGTACTTGA TGATGGACTT CAGCCGGTCC ACGATGAAGA
  421 AGTGCTTGTC GTCGTTGTAG TAGCCCAGAT CGCCGCTGTG CAGCCAGCCG TCGTTGTCGA
  481 TGATGGCGTT GGTGGCCTCG ATGTTGTTGC AGTAGCCCTT CATGATCATG TCGCCCTTGA
  541 AGCACAGCTC GCCCACTTCA TTAGGGCCCA GGGCCTTGCC GGTCCGGTGG TCGATGATCT
  601 TGGCGGCGAA GAATGGCACC ACCTTGCCGA TGCNNNNGGG CACTGTCTCG CTCTCGGGGG
  661 TGATCAGCAC GGCGCTGGTT GTCTCGGTCA GGCCGTAGCC CTGCCGGATG GCGTTCAGCT
  721 TGAACCGTCT GGCCACGGCC TCGCCGATTT CTTTGCTCAG GGGGGCTCCG CCGCTGGCGA
  781 TCTCTTTCAN GTANGGCAGG TTGTACTTGT CCACCANGGC GCTCTTGGCG AANAANGTCA
  841 TCANGGNANG CACCAGCANG GNGCTTTCCA CCTNNNNATC TTNNAGGGNC TGCANGANCA
901 GCTTCNCGTA NAANNNNGNG NNTCANCACN NNNCNGNNNN NNNNNN
//
Primer: Luc2-M_F (Pr1049)
Region: insert mid
Sequence file: RDB14363_A6Jce.seq check
>14363_14363_B1IoF_Luc2-M_F_E02_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNTTCN NNNNCNTCAT CAGCCCCGGC ACCGCCATCC
   61 TGACCGTGAT CCCTTTCCAC CACGGCTTCG GCATGTTCAC CACCCTGGGC TACTTCACCT
  121 GTGGCTTCCG GATCGTGCTG ATGCACACCT TCTACGAGAA GCTGTTCCTG CAGGCCCTGG
  181 AAGATTACAA GGTGGAAAGC ACCCTGCTGG TGCCTACCCT GATGACCTTC TTCGCCAAGA
  241 GCGCCCTGGT GGACAAGTAC AACCTGCCCT ACCTGAAAGA GATCGCCAGC GGCGGAGCCC
  301 CCCTGAGCAA AGAAATCGGC GAGGCCGTGG CCAGACGGTT CAAGCTGAAC GCCATCCGGC
  361 AGGGCTACGG CCTGACCGAG ACAACCAGCG CCGTGCTGAT CACCCCCGAG AGCGAGACAG
  421 TGCCCGGCAG CATCGGCAAG GTGGTGCCAT TCTTCGCCGC CAAGATCATC GACCACCGGA
  481 CCGGCAAGGC CCTGGGCCCT AATGAAGTGG GCGAGCTGTG CTTCNNNGGC GACATGATCA
  541 TGANGGGCTA CTGCAACNNC ATCGAGGCCA CCNACGCCAT CATCGACAAC GACGGCTGGC
  601 TGCACAGCNG CGATCTGGGC TACTACNACG ANNACNANCA CTTCNTCATC NTGGANCGGC
  661 TGANNTCNNT CATCNAGTAC NNGGGNTANN NNGTGGNCCC CNNNCNNANC NNNNNNNANN
721 NNGNNNNNNN NCNNNCNNNN NNNNNN
//
Primer: Luc2-M_R (Pr1050)
Region: insert mid
Sequence file: RDB14363_A6Jcf.seq check
>14363_14363_B1IoF_Luc2-M_R_F02_06_ABI08.ab1
    1 NNNNNNNNNN NNGNNNNNNN NCTTGGCGGC GNANANNGGC ACCACCTTGC CGATGCTGCC
   61 GGGCACTGTC TCGCTCTCGG GGGTGATCAG CACGGCGCTG GTTGTCTCGG TCAGGCCGTA
  121 GCCCTGCCGG ATGGCGTTCA GCTTGAACCG TCTGGCCACG GCCTCGCCGA TTTCTTTGCT
  181 CAGGGGGGCT CCGCCGCTGG CGATCTCTTT CAGGTAGGGC AGGTTGTACT TGTCCACCAG
  241 GGCGCTCTTG GCGAAGAAGG TCATCAGGGT AGGCACCAGC AGGGTGCTTT CCACCTTGTA
  301 ATCTTCCAGG GCCTGCAGGA ACAGCTTCTC GTAGAAGGTG TGCATCAGCA CGATCCGGAA
  361 GCCACAGGTG AAGTAGCCCA GGGTGGTGAA CATGCCGAAG CCGTGGTGGA AAGGGATCAC
  421 GGTCAGGATG GCGGTGCCGG GGCTGATGAT GTTGCCGAAC ACGGGGTCCT TGCAGTGGCT
  481 GAAGCGCACC ACCAGGTTCT TGTGGGTCAG CATCACGCCC TTGGGCAGGC CGGTGCTGCC
  541 GCTGCTGTTC AGGATCAGGC CGACCTGTTC GTTCCGGTTG AAGCTGCTGG GCTTGAACTG
  601 GTAGGCGTCG AAGTCCTTGT TGCAGTTGNG GCTNANGAAG TTGTCCAGAC ACTGAGCNNG
  661 CTCGCTGNCG TGNTTGCTGT CCANCACGAN GANTTCNTTN NNGNANNNNN NCNTNNNNNN
721 GANCNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ogoh, K., Cloning and mutagenetic modification of the firefly luciferase gene and its use for bioluminescence microscopy of engrailed expression during Drosophila metamorphosis. Biochem. Biophys. Rep. 23: 100771 (2020). PMID 32490216. [link to RRC of NBRP]
original Akiyoshi, R., FIREFLY LUCIFERASE. Patent publication US2015/0291938 A1. [link to RRC of NBRP]
review Suzuki, H., Bioluminescence microscopy for the visualization of gene expression patterns and the study of embryonic development. Proc. Arthropod. Embryol. Soc. Jpn. 53: 1-7 (2021).