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pPsagRE1Luc

Red-shifted luciferase of Pristolycus sagulatus firefly with higher luminous intensity.

Catalog number RDB14361
Resource name pPsagRE1Luc
Clone info. Red-shifted luciferase of Pristolycus sagulatus firefly with higher luminous intensity. A maximum luminescent wavelength falls 609 nm (Fig. 11 of Ogoh K, Akiyoshi R, Suzuki H. 2013. Firefly luciferase. Japan patent publication 2013-138668). pPsagRE1Luc is not expression vector. When the luciferase is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Pristolycus sagulatus Luciferase cDNA
Depositor|Developer OLYMPUS CORPORATION, | Ogoh, Katsunori |
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External Database
Pristolycus sagulatus Luciferase

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Ogoh K, Akiyoshi R, Suzuki H. 2013. Firefly luciferase. Japan patent publication 2013-138668). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
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          提供条件 利用者は、研究成果の公表にあたって別紙に定める寄託者の指定する文献を引用する (ホタル由来ルシフェラーゼ 特開2013-138668)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び商業活動(*)を行う場合は、オリンパス株式会社と別途協議を行う。(*)商用活動とは,本件リソースが製品の一部に組み込まれる場合,本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。そのための基礎検討の使用に関しては,営利,非営利機関等の利用者制限は定めない。
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          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB14361 pPsagRE1Luc DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Ogoh, K., Firefly luciferase. Japan Patent Abstracts 2013-138668.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Higher intensity luciferase (English text)
          Featured content Higher intensity luciferase (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14361_A6Jcp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: Reverse2
          Sequence file: RDB14361_A6Jca.seq check
          >D03707A3_A6Jc_3_Reverse2_F04_16_ABI24.ab1
              1 ATCAGGATTA CGCCAAGCTT GCATGCCTGC AGGTCGACTC TAGAGGATCC GATGGACAAC
             61 GACAAGAACA TCGTGTACGG CCCCGAGCCC TTCTACCCCG TGGATAAGGG ATCTGCCGGC
            121 GCTCAGATCC ACAAGTACCT GCACCAGTAC AGCAAGCTGG GCGCCATTGC CTTCACCAAT
            181 GCCCTGACCG GCGTGGACAT CACCTACGCC GAGTACTTCG ACATCACCTG TCGGCTGGGC
            241 GAGGCCATGA AGAGATACGG CATCAAGGTG GACGGCCGGA TCGCCCTGTG CAGCGAGAAC
            301 TGCGAAGAGT TCTTCTACCC TGTGATCGCC GGCCTGTACA TCGGAGCTGG CGTGGCCCCC
            361 ACCAACGAGA TCTACACCCT GCGGGAACTG GTGCACAGCC TGGGCATCAG CAAGCCCACC
            421 ATCGTGTTCA GCAGCAAGAA AGGCCTGGAC AAGGTGCTGC ATGTGCAGAA AACCGTGACC
            481 TGCATCAAGA CCGTCGTGAT CCTGGACAGC AAGGTGGACT ACAAGGGCTA CGACTGCGTG
            541 GAATCCTTCA TCAAGCGGTA CAACCCCCCA GGCTTCCAGG CCGCCAGCTT CAAGACCGTG
            601 GAAGTGGACC GGAAAGAGCA GATCGCCCTG ATCATGAACA GCAGCGGCAG CACCGGCCTG
            661 CCTAAAGGCG TGCAGCTGAC ACACGAGTGC GCCGTGACCA GATTCAGCCA CGCCAAGGAC
            721 CCCATCTACG GCAACCAGGT GTCCCCTGGA ACCGCCGTGC TGACCGTGGT GCCTTTCCAC
            781 CACGGCTTCG GCATGTTCAC CACCCTGGGC TATCTGGCCT GCGGCTTCAG AGTGGTCATG
            841 CTGACCCGCT TCGACGAGGA AATCTTCCTG AAAACCATCC AGGACTACAA GTGCACCCTA
            901 GTGATCCTGG TGCCCACCCT GTTCGGCATC CTGAGCAAGA GCAAGCTGCT GGATAAGTAC
            961 GACCCTGAGC AACCCTGCTG GAAATCGCTC TGGGCGGGAG CCCCCCCCTG GTCAAAGGAG
           1021 GTGTCCTGAG CCGTCGTCAG AACCGCTCCA TCTGCCCTTG CGTGCGTCAT ACTTACGAAC
          1081 TGACAGAGAA CGATCG
          //
          Primer: pUC-M13_-100
          Sequence file: RDB14361_A6Jcb.seq check
          >D03707A3_A6Jc_3_pUC-M13_-100_E04_13_ABI24.ab1
              1 GGCGGTCGAG CGAATTAAGT TGGGTACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC
             61 GACGGCCAGT GAATTCTCAC ATCTTGCTCT TGGGCTTCAT CAGGATCTCG CGGATGGCCT
            121 TGCCGTCGAT CTTGCCGGTC AGGCCCTTAG GCACTTCATC CACGAAGCGC ACGCCGGCTC
            181 TCAGTCTCTT GGCGTTGGAC ACCTGTCTGG CCACGTAGTC CATGATTTCT TTCTCGGTCA
            241 CGTGCTTGCC CTTTTCCAGC ACCACGACAG CCCCAGGCAG TTCGCCTGCT TCAGCATCTG
            301 GCACCCCGGC CACGCCGGCA TCGATGATGT AGGGATGCTG CAGCAGCACA GATTCCAGCT
            361 CGGCTGGGGG CACCTGGTAG CCCTTATACT TGATCAGGGA CTTCAGCCGG TCCACGATGA
            421 AGAAGTGCTG GTCCTCGTCG TAGTAGCCGA TGTCGCCTGT GTGCAGCCAG CCGTCCTCGT
            481 CGATGATCTC TCTGGTGGCC TCGGGGTTGT CCATGTAGCC CTTCATCAGC ATGGGGCCCT
            541 TCACACAGAC CTCGCCCCGC TGGTTGGGGC CCAGGGTTTT CTTGGTGTCC AGGTCCACCA
            601 CCTTGGCCTT GAACAGGGGC ACCACCTTTC CAGAGGCGCC AGGCTTATCG TCGCCCTCGG
            661 GGGTGATGAT GATGGCGCTG GTTGTCTCTG TCAGTCCGTA GCCCTGCCGC ACGCCAGGCA
            721 GGTTGAACCG TCTGGCGACG GCTTCAGACA CTTCTTTGGC CAGGGGGGCT CCGCCAGAGG
            781 CGATTTCCAC CAGGTTGCTC AGGTCGTACT TATCCAGCAG CTTGCTCTTG CTCAGATGCC
            841 GAACAGGGTG GGCACCAGGA TCACTAGGGT GCACTTGTAG TCCTGGATGG TTTTCAGGAA
            901 GATTTCCTCG TCGAAGCGGG TCAGCATGAC CACTCTGAAG CCGGCAGGCC AGATAGCCCA
            961 GGGTGGGTGA ACATGCCGAA AGCCGTGGTG GAAGGGCACC ACGTCAGCAC GGCGGGTTCC
           1021 CAGGGACACC TGATTTGCCC GTAGATTGGG GTTCCTTGCG TACTGATCCT GATCACGACG
          1081 CCACTCGTAT GTCCAGCTGT CCACCGTCCC T
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ogoh, K., Firefly luciferase. Japan Patent Abstracts 2013-138668.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl