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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pPsagRE1Luc (#RDB14361)

Red-shifted luciferase of Pristolycus sagulatus firefly with higher luminous intensity.

Clone info. Red-shifted luciferase of Pristolycus sagulatus firefly with higher luminous intensity. A maximum luminescent wavelength falls 609 nm (Fig. 11 of Ogoh K, Akiyoshi R, Suzuki H. 2013. Firefly luciferase. Japan patent publication 2013-138668). pPsagRE1Luc is not expression vector. When the luciferase is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Pristolycus sagulatus Luciferase cDNA
Depositor|Developer OLYMPUS CORPORATION, | Ogoh, Katsunori |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Ogoh K, Akiyoshi R, Suzuki H. 2013. Firefly luciferase. Japan patent publication 2013-138668). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって別紙に定める寄託者の指定する文献を引用する (ホタル由来ルシフェラーゼ　特開2013-138668)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び商業活動(*)を行う場合は、オリンパス株式会社と別途協議を行う。(*)商用活動とは，本件リソースが製品の一部に組み込まれる場合，本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。そのための基礎検討の使用に関しては，営利，非営利機関等の利用者制限は定めない。

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RDB14361 pPsagRE1Luc DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pPsagRE1Luc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14361).

Reference section:

Ogoh, K., Akiyoshi, R., Suzuki, H., Cloning and mutagenetic modification of the firefly luciferase gene and its use for bioluminescence microscopy of engrailed expression during Drosophila metamorphosis. Biochem. Biophys. Rep. 23: 100771 (2020). PMID 32490216. [link to RRC of NBRP]
Ogoh, K., Akiyoshi, R., Suzuki, H., Firefly luciferase. Japan Patent Abstracts 2013-138668. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14361_A6Jcp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Reverse2
Sequence file: RDB14361_A6Jca.seq check
>D03707A3_A6Jc_3_Reverse2_F04_16_ABI24.ab1
    1 ATCAGGATTA CGCCAAGCTT GCATGCCTGC AGGTCGACTC TAGAGGATCC GATGGACAAC
   61 GACAAGAACA TCGTGTACGG CCCCGAGCCC TTCTACCCCG TGGATAAGGG ATCTGCCGGC
  121 GCTCAGATCC ACAAGTACCT GCACCAGTAC AGCAAGCTGG GCGCCATTGC CTTCACCAAT
  181 GCCCTGACCG GCGTGGACAT CACCTACGCC GAGTACTTCG ACATCACCTG TCGGCTGGGC
  241 GAGGCCATGA AGAGATACGG CATCAAGGTG GACGGCCGGA TCGCCCTGTG CAGCGAGAAC
  301 TGCGAAGAGT TCTTCTACCC TGTGATCGCC GGCCTGTACA TCGGAGCTGG CGTGGCCCCC
  361 ACCAACGAGA TCTACACCCT GCGGGAACTG GTGCACAGCC TGGGCATCAG CAAGCCCACC
  421 ATCGTGTTCA GCAGCAAGAA AGGCCTGGAC AAGGTGCTGC ATGTGCAGAA AACCGTGACC
  481 TGCATCAAGA CCGTCGTGAT CCTGGACAGC AAGGTGGACT ACAAGGGCTA CGACTGCGTG
  541 GAATCCTTCA TCAAGCGGTA CAACCCCCCA GGCTTCCAGG CCGCCAGCTT CAAGACCGTG
  601 GAAGTGGACC GGAAAGAGCA GATCGCCCTG ATCATGAACA GCAGCGGCAG CACCGGCCTG
  661 CCTAAAGGCG TGCAGCTGAC ACACGAGTGC GCCGTGACCA GATTCAGCCA CGCCAAGGAC
  721 CCCATCTACG GCAACCAGGT GTCCCCTGGA ACCGCCGTGC TGACCGTGGT GCCTTTCCAC
  781 CACGGCTTCG GCATGTTCAC CACCCTGGGC TATCTGGCCT GCGGCTTCAG AGTGGTCATG
  841 CTGACCCGCT TCGACGAGGA AATCTTCCTG AAAACCATCC AGGACTACAA GTGCACCCTA
  901 GTGATCCTGG TGCCCACCCT GTTCGGCATC CTGAGCAAGA GCAAGCTGCT GGATAAGTAC
  961 GACCCTGAGC AACCCTGCTG GAAATCGCTC TGGGCGGGAG CCCCCCCCTG GTCAAAGGAG
 1021 GTGTCCTGAG CCGTCGTCAG AACCGCTCCA TCTGCCCTTG CGTGCGTCAT ACTTACGAAC
1081 TGACAGAGAA CGATCG
//
Primer: pUC-M13_-100
Sequence file: RDB14361_A6Jcb.seq check
>D03707A3_A6Jc_3_pUC-M13_-100_E04_13_ABI24.ab1
    1 GGCGGTCGAG CGAATTAAGT TGGGTACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC
   61 GACGGCCAGT GAATTCTCAC ATCTTGCTCT TGGGCTTCAT CAGGATCTCG CGGATGGCCT
  121 TGCCGTCGAT CTTGCCGGTC AGGCCCTTAG GCACTTCATC CACGAAGCGC ACGCCGGCTC
  181 TCAGTCTCTT GGCGTTGGAC ACCTGTCTGG CCACGTAGTC CATGATTTCT TTCTCGGTCA
  241 CGTGCTTGCC CTTTTCCAGC ACCACGACAG CCCCAGGCAG TTCGCCTGCT TCAGCATCTG
  301 GCACCCCGGC CACGCCGGCA TCGATGATGT AGGGATGCTG CAGCAGCACA GATTCCAGCT
  361 CGGCTGGGGG CACCTGGTAG CCCTTATACT TGATCAGGGA CTTCAGCCGG TCCACGATGA
  421 AGAAGTGCTG GTCCTCGTCG TAGTAGCCGA TGTCGCCTGT GTGCAGCCAG CCGTCCTCGT
  481 CGATGATCTC TCTGGTGGCC TCGGGGTTGT CCATGTAGCC CTTCATCAGC ATGGGGCCCT
  541 TCACACAGAC CTCGCCCCGC TGGTTGGGGC CCAGGGTTTT CTTGGTGTCC AGGTCCACCA
  601 CCTTGGCCTT GAACAGGGGC ACCACCTTTC CAGAGGCGCC AGGCTTATCG TCGCCCTCGG
  661 GGGTGATGAT GATGGCGCTG GTTGTCTCTG TCAGTCCGTA GCCCTGCCGC ACGCCAGGCA
  721 GGTTGAACCG TCTGGCGACG GCTTCAGACA CTTCTTTGGC CAGGGGGGCT CCGCCAGAGG
  781 CGATTTCCAC CAGGTTGCTC AGGTCGTACT TATCCAGCAG CTTGCTCTTG CTCAGATGCC
  841 GAACAGGGTG GGCACCAGGA TCACTAGGGT GCACTTGTAG TCCTGGATGG TTTTCAGGAA
  901 GATTTCCTCG TCGAAGCGGG TCAGCATGAC CACTCTGAAG CCGGCAGGCC AGATAGCCCA
  961 GGGTGGGTGA ACATGCCGAA AGCCGTGGTG GAAGGGCACC ACGTCAGCAC GGCGGGTTCC
 1021 CAGGGACACC TGATTTGCCC GTAGATTGGG GTTCCTTGCG TACTGATCCT GATCACGACG
1081 CCACTCGTAT GTCCAGCTGT CCACCGTCCC T
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ogoh, K., Cloning and mutagenetic modification of the firefly luciferase gene and its use for bioluminescence microscopy of engrailed expression during Drosophila metamorphosis. Biochem. Biophys. Rep. 23: 100771 (2020). PMID 32490216. [link to RRC of NBRP]
original Ogoh, K., Firefly luciferase. Japan Patent Abstracts 2013-138668. [link to RRC of NBRP]
review Suzuki, H., Bioluminescence microscopy for the visualization of gene expression patterns and the study of embryonic development. Proc. Arthropod. Embryol. Soc. Jpn. 53: 1-7 (2021).