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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDkumLuc1

Luciferase of Drilaster kumejimensis firefly with higher luminous intensity.

Catalog number RDB14360
Resource name pDkumLuc1
Clone info. Luciferase of Drilaster kumejimensis firefly with higher luminous intensity. A maximum luminous wavelength falls within a range of 564 to 577 nm over the temperature of 25 to 37 centigrade (Fig. 5 of Akiyoshi R, Ogoh K, Suzuki H. 2013. Firefly luciferase. Japan patent publication 2013-74861). pDkumLuc1 is not expression vector. When the luciferase is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Drilaster kumejimensis Luciferase cDNA
Depositor|Developer OLYMPUS CORPORATION, | Ogoh, Katsunori |
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External Database
Drilaster kumejimensis Luciferase

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Akiyoshi R, Ogoh K, Suzuki H. 2013. Firefly luciferase. Japan patent publication 2013-74861). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって別紙に定める寄託者の指定する文献を引用する (ホタル由来ルシフェラーゼ 特開2013-74861)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び商業活動(*)を行う場合は、オリンパス株式会社と別途協議を行う。(*)商用活動とは,本件リソースが製品の一部に組み込まれる場合,本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。そのための基礎検討の使用に関しては,営利,非営利機関等の利用者制限は定めない。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB14360 pDkumLuc1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Akiyoshi, R., Firefly luciferase. Japan Patent Abstracts 2013-74861.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Higher intensity luciferase (English text)
          Featured content Higher intensity luciferase (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14360_A6Jcp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pUC-CAPbs_up_F
          Region: insert 5'
          Sequence file: RDB14360_A6Jcd.seq check
          >14360_14360_B2DkF_3_pUC-CAPbs_up_F_B01_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNCA ATTAATGNGA GTTAGCTCAC TCATTAGGCA
             61 CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA
            121 CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTTGCATG CCTGCAGGTC
            181 GACTCTAGAG GATCCGATGG ACATGGAAGA TAAGAACGTG GTGCACGGCC CTGCCAGCTT
            241 CTACCCTGTG GATGATGGCA CAGCCGGCCA GCAGCTGCAC AAGACCATCC TGAGATACGC
            301 CCAGCTGCCC GGCACAATCG CCTTTACCGA TGCCCACACC GAGCAGAACC TGACCTACGC
            361 CGAGTACTTC GACCTGACCT GCAGACTGGC CGAGAGCATG AAGAGATACG GCCTGGGCCT
            421 GAAGCACGCC ATTGCCGTGT GCAGCGAGAA CAGCCTGAAC TTCTTCGCCC CCGTGATCGC
            481 CGCCCTGTAT CTGGGAGTGG CCGTGGCCCC CACCAACGAC ATCTACAACG AGAGAGAGCT
            541 GTACAACTCC CTGAACATCA GCCAGCCCAC CATCGTGTTC TGCAGCAAGC GGGCCCTGCT
            601 GAAGATCATG GAAGTGCAGA AAAAGCTGCC CGTGATCCAG AAAATCATCG TGCTGGACCA
            661 CAAAGAGGAC TTCCAGGGCC TGCACAGCAT GCACAGCTTC ATCAAGCACT ACCTGCCCAG
            721 CCACTTCAAC GAGCACGACT TCNAGCCCGA AGAGTTCGAC CGGAACGACC AGGTGGCCCT
            781 GATCATGAAC AGCAGCGGCA GCACCGGCCT GCCTAAAGGC GTGATGCTGA NCCACAAGAA
            841 TGTGGTCGTG CGGTTCNNCC ACTGCANNGN NCCNNNGTTN NGNNAACCAG ATCATCCCCG
            901 ANACCGCTAT CCTGANCGNG ATCCCTTTNC NNCACGGCTT CGGCNNGTTC ACCACCCTGG
            961 GCTNNCNGAN CNGNGNNNNN CNGNNTCNNG GTCNTGNNCN ANTTCNANGA ANANCTGNNC
          1021 CTGNNNNNNN NGNNNNNNNN CNNNATCNNN NANCNCCCNN NNNNGNNNNN NNNNN
          //
          Primer: pUC-M13_-100
          Region: insert 3'
          Sequence file: RDB14360_A6Jce.seq check
          >D03707A2_A6Jc_3_pUC-M13_-100_C04_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN TNGGNNNGCN NGGNNTTTCC CNGTCACGAC GTTGTAAAAC
             61 GACGGCCAGT GAATTCTCAC ATCTTGCTCT GGGGCCGCTT CAGGATTTCT TTCAGGCCTC
            121 TGCTGTCGAT CTTGCCGGTC AGGCCCTTAG GGATCTCGTC GATGAAGCGC ACGCCGCCTC
            181 TCAGTTTCTT GCTGCTGGAC ACCTGGCTGG CGACGTAGTC CATCACTTCT TTCTCGGTCA
            241 GGTGCTTGCC CTTTTCCAGC ACCACACAGG CGGCAGGCAG TTCGCCTGCC ACTTCATCAG
            301 GGATGCCGGT CACGCCAGCG TCGAAGATGT AGGGATGCTG CAGCAGCACG CTTTCCAGCT
            361 CGGCAGGGGC CACCTGATAG CCCTTGTACT TGATCAGGGA CTTCAGCCGG TCCACGATGA
            421 AGAAGTGGCC GTCCTCGTCG TAGTAGGCCA GGTCGCCGCT GTGCAGCCAG CCATCCTTGT
            481 CGATCAGGGC GTTGGTGGCC TCGGGGTTGT TGGCGTAGCC CTTCATGATC ATGTCGCCCT
            541 TCAGGCACAG CTCGCCTTTC TGGTGAGGGC CCAGGCTCTG TCTGGTGTCC AGGTCGATGA
            601 TCTTGGCGCT GAAGAATGGC ACCACCTTGC CTGTAGAGCC GGCCTTATCT TCGCCCTCGG
            661 GTGTGATGAT CACGGCGCTG GTTGTCTCTG TCAGTCCGTA GCCCTGCCGC ACGCCAGGCA
            721 GCTTAAACCG CTTGGCGACN GCCTCTCCCA CTTCTTTAGC CAGGGGGGCT CCGCCGCNGG
            781 CGATCTCTTT CNNNTTGCTC NNNCGTACTT ATCCACCAGG GNGCTCTTGG CGAANAANCT
            841 GAACANGGTA NGCACCAGCN NGGCGCNCNN GATCTTGTAN NNCNGCNNNN TCCNNNNNNN
          901 ANTTCNTCCN NNNNCTTGNN CATGANCNCN NTCCNGNAAG NNNNNNNNNN
          //
          Primer: RDB14360_F1
          Region: insert mid
          Sequence file: RDB14360_A6Jcf.seq check
          >14360_14360_B2DkF_3_RDB14360_F1_D01_04_ABI08.ab1
              1 ANNNNNNNNN NNNNCNNNNN NNCNTCGTGC TGGACCACAA AGAGGACTTC CAGGGCCTGC
             61 ACAGCATGCA CAGCTTCATC AAGCACTACC TGCCCAGCCA CTTCAACGAG CACGACTTCA
            121 AGCCCGAAGA GTTCGACCGG AACGACCAGG TGGCCCTGAT CATGAACAGC AGCGGCAGCA
            181 CCGGCCTGCC TAAAGGCGTG ATGCTGACCC ACAAGAATGT GGTCGTGCGG TTCAGCCACT
            241 GCAAGGACCC CGTGTTCGGC AACCAGATCA TCCCCGACAC CGCTATCCTG ACCGTGATCC
            301 CTTTCCACCA CGGCTTCGGC ATGTTCACCA CCCTGGGCTA CCTGACCTGT GGCTTCCGGA
            361 TCGTGGTCAT GCACAAGTTC GAGGAAGAAC TGTTCCTGCG GAGCCTGCAG GACTACAAGA
            421 TCCAGAGCGC CCTGCTGGTG CCTACCCTGT TCAGCTTCTT CGCCAAGAGC ACCCTGGTGG
            481 ATAAGTACGA CCTGAGCAAC CTGAAAGAGA TCGCCAGCGG CGGAGCCCCC CTGGCTAAAG
            541 AAGTGGGAGA GGCCGTCGCC AAGCGGTTTA AGCTGCCTGG CGTGCGGCAG GGCTACGGAC
            601 TGACAGAGAC AACCAGCGCC GTGATCATCA CACCCGANGG CGAANANAAN GNCGGCTCTA
          661 CNGGCAAGGN GGTGNCNTTC TTCNNCGNCN ANNTCATCGA NNNNNNNNNN
          //
          Primer: Luc2-M_F
          Region: insert mid
          Sequence file: RDB14360_A6Jcg.seq check
          >14360_14360_B2DkF_3_Luc2-M_F_A01_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNAGGNN NCCGNGTTCG GNNNCCNGAT CATCCCCGAC ACCGCTATCC
             61 TGACCGTGAT CCCTTTCCAC CACGGCTTCG GCATGTTCAC CACCCTGGGC TACCTGACCT
            121 GTGGCTTCCG GATCGTGGTC ATGCACAAGT TCGAGGAAGA ACTGTTCCTG CGGAGCCTGC
            181 AGGACTACAA GATCCAGAGC GCCCTGCTGG TGCCTACCCT GTTCAGCTTC TTCGCCAAGA
            241 GCACCCTGGT GGATAAGTAC GACCTGAGCA ACCTGAAAGA GATCGCCAGC GGCGGAGCCC
            301 CCCTGGCTAA AGAAGTGGGA GAGGCCGTCG CCAAGCGGTT TAAGCTGCCT GGCGTGCGGC
            361 AGGGCTACGG ACTGACAGAG ACAACCAGCG CCGTGATCAT CACACCCGAG GGCGAAGATA
            421 AGGCCGGCTC TACAGGCAAG GTGGTGCCAT TCTTCAGCGC CAAGATCATC GACCTGGACA
            481 CCAGACAGAG CCTGGGCCCT CACCAGAAAG GCGAGCTGTG CCTGAAGGGC GACATGATCA
            541 TGAAGGGCTA CGCCAACAAC CCCGAGGCCA CCAACGCCCT GATCGACAAG GATGGCTGGC
            601 TGCACAGCGG CGACCTGGCC TACTACGACG AGGACGGCCA CTTCTTCATC GTGGACCGGC
            661 TGAAGTCCCT GATCAAGTAC AAGGGCTATC AGGTGGCCCC TGCCGAGCTG GAAAGCGTGC
            721 TGCTGCAGCA TCCCTACATC TTCGACGCTG GCGTGACCGG CATCCCTGAN GAAGTGGCAG
            781 GCGAACTGCC TGCCGCCTGT GTGGTGCTGG AAAAGGGCAA GCACCTGACC GAGAAAGAAG
            841 TGATGGACTA CGTCNCCNGC CAGGTGTCCA GCAGCAAGAA ACTGANANGC GGCGNGCGCT
            901 TCNTCGACGA NATCCCTAAG GGNCTGANCG GCAAGNTCGA CAGCANANGN CNGAAAGAAN
            961 TCCTGAAGCG GCCCCNNAGC ANGATGNGAG AANTTCACTG GNNNGTCNTT TTACNACGTC
          1021 NNGACTGGGA AANNCCNNNN NNNNNCCNNC NNNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akiyoshi, R., Firefly luciferase. Japan Patent Abstracts 2013-74861.

          2022.05.02

          GNP_filter3_RDBDEP_html_220427.pl