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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDkumLuc1 (#RDB14360)

Luciferase of Drilaster kumejimensis firefly with higher luminous intensity.

Clone info. Luciferase of Drilaster kumejimensis firefly with higher luminous intensity. A maximum luminous wavelength falls within a range of 564 to 577 nm over the temperature of 25 to 37 centigrade (Fig. 5 of Akiyoshi R, Ogoh K, Suzuki H. 2013. Firefly luciferase. Japan patent publication 2013-74861). pDkumLuc1 is not expression vector. When the luciferase is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Drilaster kumejimensis Luciferase cDNA
Depositor|Developer OLYMPUS CORPORATION, | Ogoh, Katsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Akiyoshi R, Ogoh K, Suzuki H. 2013. Firefly luciferase. Japan patent publication 2013-74861). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって別紙に定める寄託者の指定する文献を引用する (ホタル由来ルシフェラーゼ　特開2013-74861)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び商業活動(*)を行う場合は、オリンパス株式会社と別途協議を行う。(*)商用活動とは，本件リソースが製品の一部に組み込まれる場合，本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。そのための基礎検討の使用に関しては，営利，非営利機関等の利用者制限は定めない。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14360 pDkumLuc1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDkumLuc1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14360).

Reference section:

Ogoh, K., Akiyoshi, R., Suzuki, H., Cloning and mutagenetic modification of the firefly luciferase gene and its use for bioluminescence microscopy of engrailed expression during Drosophila metamorphosis. Biochem. Biophys. Rep. 23: 100771 (2020). PMID 32490216. [link to RRC of NBRP]
Akiyoshi, R., Ogoh, K., Suzuki, H., Firefly luciferase. Japan Patent Abstracts 2013-74861. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14360_A6Jcp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-CAPbs_up_F (Pr0536)
Region: insert 5'
Sequence file: RDB14360_A6Jcd.seq check
>14360_14360_B2DkF_3_pUC-CAPbs_up_F_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNCA ATTAATGNGA GTTAGCTCAC TCATTAGGCA
   61 CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA
  121 CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTTGCATG CCTGCAGGTC
  181 GACTCTAGAG GATCCGATGG ACATGGAAGA TAAGAACGTG GTGCACGGCC CTGCCAGCTT
  241 CTACCCTGTG GATGATGGCA CAGCCGGCCA GCAGCTGCAC AAGACCATCC TGAGATACGC
  301 CCAGCTGCCC GGCACAATCG CCTTTACCGA TGCCCACACC GAGCAGAACC TGACCTACGC
  361 CGAGTACTTC GACCTGACCT GCAGACTGGC CGAGAGCATG AAGAGATACG GCCTGGGCCT
  421 GAAGCACGCC ATTGCCGTGT GCAGCGAGAA CAGCCTGAAC TTCTTCGCCC CCGTGATCGC
  481 CGCCCTGTAT CTGGGAGTGG CCGTGGCCCC CACCAACGAC ATCTACAACG AGAGAGAGCT
  541 GTACAACTCC CTGAACATCA GCCAGCCCAC CATCGTGTTC TGCAGCAAGC GGGCCCTGCT
  601 GAAGATCATG GAAGTGCAGA AAAAGCTGCC CGTGATCCAG AAAATCATCG TGCTGGACCA
  661 CAAAGAGGAC TTCCAGGGCC TGCACAGCAT GCACAGCTTC ATCAAGCACT ACCTGCCCAG
  721 CCACTTCAAC GAGCACGACT TCNAGCCCGA AGAGTTCGAC CGGAACGACC AGGTGGCCCT
  781 GATCATGAAC AGCAGCGGCA GCACCGGCCT GCCTAAAGGC GTGATGCTGA NCCACAAGAA
  841 TGTGGTCGTG CGGTTCNNCC ACTGCANNGN NCCNNNGTTN NGNNAACCAG ATCATCCCCG
  901 ANACCGCTAT CCTGANCGNG ATCCCTTTNC NNCACGGCTT CGGCNNGTTC ACCACCCTGG
  961 GCTNNCNGAN CNGNGNNNNN CNGNNTCNNG GTCNTGNNCN ANTTCNANGA ANANCTGNNC
1021 CTGNNNNNNN NGNNNNNNNN CNNNATCNNN NANCNCCCNN NNNNGNNNNN NNNNN
//
Primer: pUC-M13_-100 (Pr0310)
Region: insert 3'
Sequence file: RDB14360_A6Jce.seq check
>D03707A2_A6Jc_3_pUC-M13_-100_C04_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN TNGGNNNGCN NGGNNTTTCC CNGTCACGAC GTTGTAAAAC
   61 GACGGCCAGT GAATTCTCAC ATCTTGCTCT GGGGCCGCTT CAGGATTTCT TTCAGGCCTC
  121 TGCTGTCGAT CTTGCCGGTC AGGCCCTTAG GGATCTCGTC GATGAAGCGC ACGCCGCCTC
  181 TCAGTTTCTT GCTGCTGGAC ACCTGGCTGG CGACGTAGTC CATCACTTCT TTCTCGGTCA
  241 GGTGCTTGCC CTTTTCCAGC ACCACACAGG CGGCAGGCAG TTCGCCTGCC ACTTCATCAG
  301 GGATGCCGGT CACGCCAGCG TCGAAGATGT AGGGATGCTG CAGCAGCACG CTTTCCAGCT
  361 CGGCAGGGGC CACCTGATAG CCCTTGTACT TGATCAGGGA CTTCAGCCGG TCCACGATGA
  421 AGAAGTGGCC GTCCTCGTCG TAGTAGGCCA GGTCGCCGCT GTGCAGCCAG CCATCCTTGT
  481 CGATCAGGGC GTTGGTGGCC TCGGGGTTGT TGGCGTAGCC CTTCATGATC ATGTCGCCCT
  541 TCAGGCACAG CTCGCCTTTC TGGTGAGGGC CCAGGCTCTG TCTGGTGTCC AGGTCGATGA
  601 TCTTGGCGCT GAAGAATGGC ACCACCTTGC CTGTAGAGCC GGCCTTATCT TCGCCCTCGG
  661 GTGTGATGAT CACGGCGCTG GTTGTCTCTG TCAGTCCGTA GCCCTGCCGC ACGCCAGGCA
  721 GCTTAAACCG CTTGGCGACN GCCTCTCCCA CTTCTTTAGC CAGGGGGGCT CCGCCGCNGG
  781 CGATCTCTTT CNNNTTGCTC NNNCGTACTT ATCCACCAGG GNGCTCTTGG CGAANAANCT
  841 GAACANGGTA NGCACCAGCN NGGCGCNCNN GATCTTGTAN NNCNGCNNNN TCCNNNNNNN
901 ANTTCNTCCN NNNNCTTGNN CATGANCNCN NTCCNGNAAG NNNNNNNNNN
//
Primer: RDB14360_F1 (Pr1203)
Region: insert mid
Sequence file: RDB14360_A6Jcf.seq check
>14360_14360_B2DkF_3_RDB14360_F1_D01_04_ABI08.ab1
    1 ANNNNNNNNN NNNNCNNNNN NNCNTCGTGC TGGACCACAA AGAGGACTTC CAGGGCCTGC
   61 ACAGCATGCA CAGCTTCATC AAGCACTACC TGCCCAGCCA CTTCAACGAG CACGACTTCA
  121 AGCCCGAAGA GTTCGACCGG AACGACCAGG TGGCCCTGAT CATGAACAGC AGCGGCAGCA
  181 CCGGCCTGCC TAAAGGCGTG ATGCTGACCC ACAAGAATGT GGTCGTGCGG TTCAGCCACT
  241 GCAAGGACCC CGTGTTCGGC AACCAGATCA TCCCCGACAC CGCTATCCTG ACCGTGATCC
  301 CTTTCCACCA CGGCTTCGGC ATGTTCACCA CCCTGGGCTA CCTGACCTGT GGCTTCCGGA
  361 TCGTGGTCAT GCACAAGTTC GAGGAAGAAC TGTTCCTGCG GAGCCTGCAG GACTACAAGA
  421 TCCAGAGCGC CCTGCTGGTG CCTACCCTGT TCAGCTTCTT CGCCAAGAGC ACCCTGGTGG
  481 ATAAGTACGA CCTGAGCAAC CTGAAAGAGA TCGCCAGCGG CGGAGCCCCC CTGGCTAAAG
  541 AAGTGGGAGA GGCCGTCGCC AAGCGGTTTA AGCTGCCTGG CGTGCGGCAG GGCTACGGAC
  601 TGACAGAGAC AACCAGCGCC GTGATCATCA CACCCGANGG CGAANANAAN GNCGGCTCTA
661 CNGGCAAGGN GGTGNCNTTC TTCNNCGNCN ANNTCATCGA NNNNNNNNNN
//
Primer: Luc2-M_F (Pr1049)
Region: insert mid
Sequence file: RDB14360_A6Jcg.seq check
>14360_14360_B2DkF_3_Luc2-M_F_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNAGGNN NCCGNGTTCG GNNNCCNGAT CATCCCCGAC ACCGCTATCC
   61 TGACCGTGAT CCCTTTCCAC CACGGCTTCG GCATGTTCAC CACCCTGGGC TACCTGACCT
  121 GTGGCTTCCG GATCGTGGTC ATGCACAAGT TCGAGGAAGA ACTGTTCCTG CGGAGCCTGC
  181 AGGACTACAA GATCCAGAGC GCCCTGCTGG TGCCTACCCT GTTCAGCTTC TTCGCCAAGA
  241 GCACCCTGGT GGATAAGTAC GACCTGAGCA ACCTGAAAGA GATCGCCAGC GGCGGAGCCC
  301 CCCTGGCTAA AGAAGTGGGA GAGGCCGTCG CCAAGCGGTT TAAGCTGCCT GGCGTGCGGC
  361 AGGGCTACGG ACTGACAGAG ACAACCAGCG CCGTGATCAT CACACCCGAG GGCGAAGATA
  421 AGGCCGGCTC TACAGGCAAG GTGGTGCCAT TCTTCAGCGC CAAGATCATC GACCTGGACA
  481 CCAGACAGAG CCTGGGCCCT CACCAGAAAG GCGAGCTGTG CCTGAAGGGC GACATGATCA
  541 TGAAGGGCTA CGCCAACAAC CCCGAGGCCA CCAACGCCCT GATCGACAAG GATGGCTGGC
  601 TGCACAGCGG CGACCTGGCC TACTACGACG AGGACGGCCA CTTCTTCATC GTGGACCGGC
  661 TGAAGTCCCT GATCAAGTAC AAGGGCTATC AGGTGGCCCC TGCCGAGCTG GAAAGCGTGC
  721 TGCTGCAGCA TCCCTACATC TTCGACGCTG GCGTGACCGG CATCCCTGAN GAAGTGGCAG
  781 GCGAACTGCC TGCCGCCTGT GTGGTGCTGG AAAAGGGCAA GCACCTGACC GAGAAAGAAG
  841 TGATGGACTA CGTCNCCNGC CAGGTGTCCA GCAGCAAGAA ACTGANANGC GGCGNGCGCT
  901 TCNTCGACGA NATCCCTAAG GGNCTGANCG GCAAGNTCGA CAGCANANGN CNGAAAGAAN
  961 TCCTGAAGCG GCCCCNNAGC ANGATGNGAG AANTTCACTG GNNNGTCNTT TTACNACGTC
1021 NNGACTGGGA AANNCCNNNN NNNNNCCNNC NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ogoh, K., Cloning and mutagenetic modification of the firefly luciferase gene and its use for bioluminescence microscopy of engrailed expression during Drosophila metamorphosis. Biochem. Biophys. Rep. 23: 100771 (2020). PMID 32490216. [link to RRC of NBRP]
original Akiyoshi, R., Firefly luciferase. Japan Patent Abstracts 2013-74861. [link to RRC of NBRP]
review Suzuki, H., Bioluminescence microscopy for the visualization of gene expression patterns and the study of embryonic development. Proc. Arthropod. Embryol. Soc. Jpn. 53: 1-7 (2021).