Please review the QC test results indicated by icon below as well as clone information before placing your order.

pDkumLuc1

Luciferase of Drilaster kumejimensis firefly with higher luminous intensity.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Akiyoshi R, Ogoh K, Suzuki H. 2013. Firefly luciferase. Japan patent publication 2013-74861). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.

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Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって別紙に定める寄託者の指定する文献を引用する (ホタル由来ルシフェラーゼ　特開2013-74861)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び商業活動(*)を行う場合は、オリンパス株式会社と別途協議を行う。(*)商用活動とは，本件リソースが製品の一部に組み込まれる場合，本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。そのための基礎検討の使用に関しては，営利，非営利機関等の利用者制限は定めない。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14360_A6Jcp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-CAPbs_up_F (Pr0536) Region: insert 5' Sequence file: RDB14360_A6Jcd.seq
>14360_14360_B2DkF_3_pUC-CAPbs_up_F_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNCA ATTAATGNGA GTTAGCTCAC TCATTAGGCA    61 CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA   121 CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTTGCATG CCTGCAGGTC   181 GACTCTAGAG GATCCGATGG ACATGGAAGA TAAGAACGTG GTGCACGGCC CTGCCAGCTT   241 CTACCCTGTG GATGATGGCA CAGCCGGCCA GCAGCTGCAC AAGACCATCC TGAGATACGC   301 CCAGCTGCCC GGCACAATCG CCTTTACCGA TGCCCACACC GAGCAGAACC TGACCTACGC   361 CGAGTACTTC GACCTGACCT GCAGACTGGC CGAGAGCATG AAGAGATACG GCCTGGGCCT   421 GAAGCACGCC ATTGCCGTGT GCAGCGAGAA CAGCCTGAAC TTCTTCGCCC CCGTGATCGC   481 CGCCCTGTAT CTGGGAGTGG CCGTGGCCCC CACCAACGAC ATCTACAACG AGAGAGAGCT   541 GTACAACTCC CTGAACATCA GCCAGCCCAC CATCGTGTTC TGCAGCAAGC GGGCCCTGCT   601 GAAGATCATG GAAGTGCAGA AAAAGCTGCC CGTGATCCAG AAAATCATCG TGCTGGACCA   661 CAAAGAGGAC TTCCAGGGCC TGCACAGCAT GCACAGCTTC ATCAAGCACT ACCTGCCCAG   721 CCACTTCAAC GAGCACGACT TCNAGCCCGA AGAGTTCGAC CGGAACGACC AGGTGGCCCT   781 GATCATGAAC AGCAGCGGCA GCACCGGCCT GCCTAAAGGC GTGATGCTGA NCCACAAGAA   841 TGTGGTCGTG CGGTTCNNCC ACTGCANNGN NCCNNNGTTN NGNNAACCAG ATCATCCCCG   901 ANACCGCTAT CCTGANCGNG ATCCCTTTNC NNCACGGCTT CGGCNNGTTC ACCACCCTGG   961 GCTNNCNGAN CNGNGNNNNN CNGNNTCNNG GTCNTGNNCN ANTTCNANGA ANANCTGNNC 1021 CTGNNNNNNN NGNNNNNNNN CNNNATCNNN NANCNCCCNN NNNNGNNNNN NNNNN //
Primer: pUC-M13_-100 (Pr0310) Region: insert 3' Sequence file: RDB14360_A6Jce.seq
>D03707A2_A6Jc_3_pUC-M13_-100_C04_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN TNGGNNNGCN NGGNNTTTCC CNGTCACGAC GTTGTAAAAC    61 GACGGCCAGT GAATTCTCAC ATCTTGCTCT GGGGCCGCTT CAGGATTTCT TTCAGGCCTC   121 TGCTGTCGAT CTTGCCGGTC AGGCCCTTAG GGATCTCGTC GATGAAGCGC ACGCCGCCTC   181 TCAGTTTCTT GCTGCTGGAC ACCTGGCTGG CGACGTAGTC CATCACTTCT TTCTCGGTCA   241 GGTGCTTGCC CTTTTCCAGC ACCACACAGG CGGCAGGCAG TTCGCCTGCC ACTTCATCAG   301 GGATGCCGGT CACGCCAGCG TCGAAGATGT AGGGATGCTG CAGCAGCACG CTTTCCAGCT   361 CGGCAGGGGC CACCTGATAG CCCTTGTACT TGATCAGGGA CTTCAGCCGG TCCACGATGA   421 AGAAGTGGCC GTCCTCGTCG TAGTAGGCCA GGTCGCCGCT GTGCAGCCAG CCATCCTTGT   481 CGATCAGGGC GTTGGTGGCC TCGGGGTTGT TGGCGTAGCC CTTCATGATC ATGTCGCCCT   541 TCAGGCACAG CTCGCCTTTC TGGTGAGGGC CCAGGCTCTG TCTGGTGTCC AGGTCGATGA   601 TCTTGGCGCT GAAGAATGGC ACCACCTTGC CTGTAGAGCC GGCCTTATCT TCGCCCTCGG   661 GTGTGATGAT CACGGCGCTG GTTGTCTCTG TCAGTCCGTA GCCCTGCCGC ACGCCAGGCA   721 GCTTAAACCG CTTGGCGACN GCCTCTCCCA CTTCTTTAGC CAGGGGGGCT CCGCCGCNGG   781 CGATCTCTTT CNNNTTGCTC NNNCGTACTT ATCCACCAGG GNGCTCTTGG CGAANAANCT   841 GAACANGGTA NGCACCAGCN NGGCGCNCNN GATCTTGTAN NNCNGCNNNN TCCNNNNNNN 901 ANTTCNTCCN NNNNCTTGNN CATGANCNCN NTCCNGNAAG NNNNNNNNNN //
Primer: RDB14360_F1 (Pr1203) Region: insert mid Sequence file: RDB14360_A6Jcf.seq
>14360_14360_B2DkF_3_RDB14360_F1_D01_04_ABI08.ab1     1 ANNNNNNNNN NNNNCNNNNN NNCNTCGTGC TGGACCACAA AGAGGACTTC CAGGGCCTGC    61 ACAGCATGCA CAGCTTCATC AAGCACTACC TGCCCAGCCA CTTCAACGAG CACGACTTCA   121 AGCCCGAAGA GTTCGACCGG AACGACCAGG TGGCCCTGAT CATGAACAGC AGCGGCAGCA   181 CCGGCCTGCC TAAAGGCGTG ATGCTGACCC ACAAGAATGT GGTCGTGCGG TTCAGCCACT   241 GCAAGGACCC CGTGTTCGGC AACCAGATCA TCCCCGACAC CGCTATCCTG ACCGTGATCC   301 CTTTCCACCA CGGCTTCGGC ATGTTCACCA CCCTGGGCTA CCTGACCTGT GGCTTCCGGA   361 TCGTGGTCAT GCACAAGTTC GAGGAAGAAC TGTTCCTGCG GAGCCTGCAG GACTACAAGA   421 TCCAGAGCGC CCTGCTGGTG CCTACCCTGT TCAGCTTCTT CGCCAAGAGC ACCCTGGTGG   481 ATAAGTACGA CCTGAGCAAC CTGAAAGAGA TCGCCAGCGG CGGAGCCCCC CTGGCTAAAG   541 AAGTGGGAGA GGCCGTCGCC AAGCGGTTTA AGCTGCCTGG CGTGCGGCAG GGCTACGGAC   601 TGACAGAGAC AACCAGCGCC GTGATCATCA CACCCGANGG CGAANANAAN GNCGGCTCTA 661 CNGGCAAGGN GGTGNCNTTC TTCNNCGNCN ANNTCATCGA NNNNNNNNNN //
Primer: Luc2-M_F (Pr1049) Region: insert mid Sequence file: RDB14360_A6Jcg.seq
>14360_14360_B2DkF_3_Luc2-M_F_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNAGGNN NCCGNGTTCG GNNNCCNGAT CATCCCCGAC ACCGCTATCC    61 TGACCGTGAT CCCTTTCCAC CACGGCTTCG GCATGTTCAC CACCCTGGGC TACCTGACCT   121 GTGGCTTCCG GATCGTGGTC ATGCACAAGT TCGAGGAAGA ACTGTTCCTG CGGAGCCTGC   181 AGGACTACAA GATCCAGAGC GCCCTGCTGG TGCCTACCCT GTTCAGCTTC TTCGCCAAGA   241 GCACCCTGGT GGATAAGTAC GACCTGAGCA ACCTGAAAGA GATCGCCAGC GGCGGAGCCC   301 CCCTGGCTAA AGAAGTGGGA GAGGCCGTCG CCAAGCGGTT TAAGCTGCCT GGCGTGCGGC   361 AGGGCTACGG ACTGACAGAG ACAACCAGCG CCGTGATCAT CACACCCGAG GGCGAAGATA   421 AGGCCGGCTC TACAGGCAAG GTGGTGCCAT TCTTCAGCGC CAAGATCATC GACCTGGACA   481 CCAGACAGAG CCTGGGCCCT CACCAGAAAG GCGAGCTGTG CCTGAAGGGC GACATGATCA   541 TGAAGGGCTA CGCCAACAAC CCCGAGGCCA CCAACGCCCT GATCGACAAG GATGGCTGGC   601 TGCACAGCGG CGACCTGGCC TACTACGACG AGGACGGCCA CTTCTTCATC GTGGACCGGC   661 TGAAGTCCCT GATCAAGTAC AAGGGCTATC AGGTGGCCCC TGCCGAGCTG GAAAGCGTGC   721 TGCTGCAGCA TCCCTACATC TTCGACGCTG GCGTGACCGG CATCCCTGAN GAAGTGGCAG   781 GCGAACTGCC TGCCGCCTGT GTGGTGCTGG AAAAGGGCAA GCACCTGACC GAGAAAGAAG   841 TGATGGACTA CGTCNCCNGC CAGGTGTCCA GCAGCAAGAA ACTGANANGC GGCGNGCGCT   901 TCNTCGACGA NATCCCTAAG GGNCTGANCG GCAAGNTCGA CAGCANANGN CNGAAAGAAN   961 TCCTGAAGCG GCCCCNNAGC ANGATGNGAG AANTTCACTG GNNNGTCNTT TTACNACGTC 1021 NNGACTGGGA AANNCCNNNN NNNNNCCNNC NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.09.13

GNP_filter3_RDBDEP_html_230702.pl