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pCAGGS/hBACE1 N153/223S

Expression vector of human BACE1mutant, N153S/N223S.

Catalog number RDB14348
Resource name pCAGGS/hBACE1 N153/223S
Alternative name pCAGGS/human BACE1 Asn153,223Ser
Clone info. Expression vector of human BACE1mutant, N153S/N223S. Point mutations at two N-glycosylation sites (Asn153 and Asn223) abolish the bisecting GlcNAc modification on BACE1.
Expression was confirmed by the depositor with western blotting and immunoprecipitation.
Vector backbone pCAGGS (plasmid)
Selectable markers Amp^r
Gene/insert name human BACE1 cDNA
Depositor Taniguchi, Naoyuki | Kizuka, Yasuhiko |
 
Other clones in our bank human BACE1 (NCBI Gene 23621) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016)
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RDB14348 pCAGGS/hBACE1 N153/223S DNA solution

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References and tips

Electronic file

Featured content

References

original Kizuka, Y., Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions. Biochem. J. 473 (1): 21-30 (2016) PMID 26467158.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14348_A6Itp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB14348_A6Ita.seq
>D03881A6_A6It_2_pAxCA_F1_D11_04_ABI08.ab1
    1 GAAAACCCTG ACTTGCTACA TGTTCATGCC TTCTTCTTTT TCCTACAGCT CCTGGGCAAC
   61 GTGCTGGTTA TTGTGCTGTC TCATCATTTT GGCAAAGAAT TCATGGCCCA AGCCCTGCCC
  121 TGGCTCCTGC TGTGGATGGG CGCGGGAGTG CTGCCTGCCC ACGGCACCCA GCACGGCATC
  181 CGGCTGCCCC TGCGCAGCGG CCTGGGGGGC GCCCCCCTGG GGCTGCGGCT GCCCCGGGAG
  241 ACCGACGAAG AGCCCGAGGA GCCCGGCCGG AGGGGCAGCT TTGTGGAGAT GGTGGACAAC
  301 CTGAGGGGCA AGTCGGGGCA GGGCTACTAC GTGGAGATGA CCGTGGGCAG CCCCCCGCAG
  361 ACGCTCAACA TCCTGGTGGA TACAGGCAGC AGTAACTTTG CAGTGGGTGC TGCCCCCCAC
  421 CCCTTCCTGC ATCGCTACTA CCAGAGGCAG CTGTCCAGCA CATACCGGGA CCTCCGGAAG
  481 GGTGTGTATG TGCCCTACAC CCAGGGCAAG TGGGAAGGGG AGCTGGGCAC CGACCTGGTA
  541 AGCATCCCCC ATGGCCCCAG CGTCACTGTG CGTGCCAACA TTGCTGCCAT CACTGAATCA
  601 GACAAGTTCT TCATCAACGG CTCCAACTGG GAAGGCATCC TGGGGCTGGC CTATGCTGAG
  661 ATTGCCAGGC CTGACGACTC CCTGGAGCCT TTCTTTGACT CTCTGGTAAA GCAGACCCAC
  721 GTTCCCAACC TCTTCTCCCT GCAGCTTTGT GGTGCTGGCT TCCCCCTCAG CCAGTCTGAA
  781 GTGCTGGCCT CTGTCGGAGG GAGCATGATC ATTGGAGGTA TCGACCACTC GCTGTACACA
  841 GGCAGTCTCT GGTATACACC CATCCGGCGG GAGTGGTATT ATGAGGTGAT CATTGTGCGG
  901 GTGGAGATCA AATGGACAGG ATCTGAAAAT GGACTGCAAG GAGTACAACT ATGACAAGAG
  961 CATTGTGGAC AGTGGCACCA CCAACCTTCG TTTGCCCAAG AAAGTGTTTG AAGCTGCAGT
 1021 CAAATCCATC AAAGGCCAGC CTTCCTCCAC GGGAGAGTTT CCCTGATGGG TTTTCTGGCT
 1081 AGGAGAGCAG CTGGTGTGCT GACAAGGCAG CCACCAACCC TGGACCATTT TTCCAGTCAA
1141 TCCTCACTCT ACCTTAAATG GGGTTGAAGG GTTACCA
//
Primer: pCA-bgter RDB14348_A6Itb.seq
>D03881A6_A6It_2_pCA-bgter_B11_05_ABI24.ab1
    1 AGCCCCCAGT AATGTCCCAG AATTTTTGGC AGAGGGAAAA AGATCTCAGT GGTATTTGTG
   61 AGCCAGGGCA TTGGCCACAC CAGCCACCAC CTTCTGATAG GCAGCCTGCA CCTGAGGAGT
  121 GAATTCTCAC TTCAGCAGGG AGATGTCATC AGCAAAGTCA TCATGCTGCT GGCGCAGGCA
  181 GCGGAGGCAG CGCCACTGAC ACACCATGAG GCAGAGTGGC AGCATGAAGA GGGCGCAGAT
  241 GGCAGCCATG ACATAGGCTA TGGTCATGAG GGTTGACTCA TCTGTCTGTG GAATGTTGTA
  301 GCCACAGTCT TCCATGTCCA AGGTGACAAA AGGGCCTTCC ACCGCTGCCG TCCTGAACTC
  361 ATCGTGCACA TGGCAAGCGC TGACAGCAAA GCCAATTCGT TTTCGGGCCC GATCAAAGAC
  421 AACGTAGAAG CCCTCCATGA TAACAGCTCC CATAACAGTG CCCGTGGATG ACTGTGAGAT
  481 GGCAAACTTG TAACAGTCGT CTTGGGACGT GGCCACATCT TCCACTGGCC GCAGGTATTG
  541 CTGCGGAAGG ATGGTGATGC GGAAGGACTG GTTGGTAACC TCACCCATTA GGTAGAGTGA
  601 GATGACTGGG AAAATGTTCC AAGGGGTGGT GCCTGCTTGC CAGCACACCA GCTGCTCTCC
  661 TAGCCAGAAA CCATCAGGGA ACTTCTCCGT GGAGGAGGCT GCCTTGATGG ATTTGACTGC
  721 AGCTTCAAAC ACTTTCTTGG GCAAACGAAG GTTGGTGGTG CCACTGTCCA CAATGCTCTT
  781 GTCATAGTTG TACTCCTTGC AGTCCATTTT CAGATCCTGT CCATTGATCT CCACCCGCAC
  841 AATGATCACC TCATAATACC ACTCCCGCCG GATGGGTGTA TACCAGAGAC TGCCTGTGTA
  901 CAGCGAGTGG TCGATACCTC CAATGATCAT GCTCCCTCCG ACAGAGGCCA GCACTTCAGA
  961 CTGGGCTGAG GGGGAAGCCA GCACCACAAA GCTGCAGGGA GAAGAGGTTG GGAACGTGGG
 1021 TCTGCTTTAC CAGAGAGTCA AAGAAAGGCT CCAGGGGAGT CGTCAAGGCC TGCAATCTCA
 1081 GCATAGCCAG CCCCAAGATG CGTCCAGTGG AGCCGTGGAT GAGACTGTCT GAATTCAGTG
1141 ATGCAGCCAA TGGTGGCACG CCAACAAGTG AACCGCCTTG GGGCCCATTG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Kizuka, Y., Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions. Biochem. J. 473 (1): 21-30 (2016) PMID 26467158.

2018.12.11

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