RDB14348 - pCAGGS/hBACE1 N153/223S

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pCAGGS/hBACE1 N153/223S (#RDB14348)

Expression vector of human BACE1mutant, N153S/N223S.

Alternative name	pCAGGS/human BACE1 Asn153,223Ser
Clone info.	Expression vector of human BACE1mutant, N153S/N223S. Point mutations at two N-glycosylation sites (Asn153 and Asn223) abolish the bisecting GlcNAc modification on BACE1.
Comment	Expression was confirmed by the depositor with western blotting and immunoprecipitation.
Vector backbone	pCAGGS (plasmid)
Selectable markers	Amp^r
Gene/insert name	human BACE1 cDNA
Depositor\|Developer	Taniguchi, Naoyuki \| Kizuka, Yasuhiko \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016)
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016) Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016)。 MTAに書く付加的使用条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016)。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14348	pCAGGS/hBACE1 N153/223S	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAGGS/hBACE1 N153/223S was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14348).

Reference section:

Kizuka, Y., Nakano, M., Kitazume, S., Saito, T., Saido, T.C., Taniguchi, N., Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions. Biochem. J. 473 (1): 21-30 (2016) PMID 26467158. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14348_A6Itp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB14348_A6Ita.seq
>D03881A6_A6It_2_pAxCA_F1_D11_04_ABI08.ab1 1 GAAAACCCTG ACTTGCTACA TGTTCATGCC TTCTTCTTTT TCCTACAGCT CCTGGGCAAC 61 GTGCTGGTTA TTGTGCTGTC TCATCATTTT GGCAAAGAAT TCATGGCCCA AGCCCTGCCC 121 TGGCTCCTGC TGTGGATGGG CGCGGGAGTG CTGCCTGCCC ACGGCACCCA GCACGGCATC 181 CGGCTGCCCC TGCGCAGCGG CCTGGGGGGC GCCCCCCTGG GGCTGCGGCT GCCCCGGGAG 241 ACCGACGAAG AGCCCGAGGA GCCCGGCCGG AGGGGCAGCT TTGTGGAGAT GGTGGACAAC 301 CTGAGGGGCA AGTCGGGGCA GGGCTACTAC GTGGAGATGA CCGTGGGCAG CCCCCCGCAG 361 ACGCTCAACA TCCTGGTGGA TACAGGCAGC AGTAACTTTG CAGTGGGTGC TGCCCCCCAC 421 CCCTTCCTGC ATCGCTACTA CCAGAGGCAG CTGTCCAGCA CATACCGGGA CCTCCGGAAG 481 GGTGTGTATG TGCCCTACAC CCAGGGCAAG TGGGAAGGGG AGCTGGGCAC CGACCTGGTA 541 AGCATCCCCC ATGGCCCCAG CGTCACTGTG CGTGCCAACA TTGCTGCCAT CACTGAATCA 601 GACAAGTTCT TCATCAACGG CTCCAACTGG GAAGGCATCC TGGGGCTGGC CTATGCTGAG 661 ATTGCCAGGC CTGACGACTC CCTGGAGCCT TTCTTTGACT CTCTGGTAAA GCAGACCCAC 721 GTTCCCAACC TCTTCTCCCT GCAGCTTTGT GGTGCTGGCT TCCCCCTCAG CCAGTCTGAA 781 GTGCTGGCCT CTGTCGGAGG GAGCATGATC ATTGGAGGTA TCGACCACTC GCTGTACACA 841 GGCAGTCTCT GGTATACACC CATCCGGCGG GAGTGGTATT ATGAGGTGAT CATTGTGCGG 901 GTGGAGATCA AATGGACAGG ATCTGAAAAT GGACTGCAAG GAGTACAACT ATGACAAGAG 961 CATTGTGGAC AGTGGCACCA CCAACCTTCG TTTGCCCAAG AAAGTGTTTG AAGCTGCAGT 1021 CAAATCCATC AAAGGCCAGC CTTCCTCCAC GGGAGAGTTT CCCTGATGGG TTTTCTGGCT 1081 AGGAGAGCAG CTGGTGTGCT GACAAGGCAG CCACCAACCC TGGACCATTT TTCCAGTCAA 1141 TCCTCACTCT ACCTTAAATG GGGTTGAAGG GTTACCA //
Primer: pCA-bgter Sequence file: RDB14348_A6Itb.seq
>D03881A6_A6It_2_pCA-bgter_B11_05_ABI24.ab1 1 AGCCCCCAGT AATGTCCCAG AATTTTTGGC AGAGGGAAAA AGATCTCAGT GGTATTTGTG 61 AGCCAGGGCA TTGGCCACAC CAGCCACCAC CTTCTGATAG GCAGCCTGCA CCTGAGGAGT 121 GAATTCTCAC TTCAGCAGGG AGATGTCATC AGCAAAGTCA TCATGCTGCT GGCGCAGGCA 181 GCGGAGGCAG CGCCACTGAC ACACCATGAG GCAGAGTGGC AGCATGAAGA GGGCGCAGAT 241 GGCAGCCATG ACATAGGCTA TGGTCATGAG GGTTGACTCA TCTGTCTGTG GAATGTTGTA 301 GCCACAGTCT TCCATGTCCA AGGTGACAAA AGGGCCTTCC ACCGCTGCCG TCCTGAACTC 361 ATCGTGCACA TGGCAAGCGC TGACAGCAAA GCCAATTCGT TTTCGGGCCC GATCAAAGAC 421 AACGTAGAAG CCCTCCATGA TAACAGCTCC CATAACAGTG CCCGTGGATG ACTGTGAGAT 481 GGCAAACTTG TAACAGTCGT CTTGGGACGT GGCCACATCT TCCACTGGCC GCAGGTATTG 541 CTGCGGAAGG ATGGTGATGC GGAAGGACTG GTTGGTAACC TCACCCATTA GGTAGAGTGA 601 GATGACTGGG AAAATGTTCC AAGGGGTGGT GCCTGCTTGC CAGCACACCA GCTGCTCTCC 661 TAGCCAGAAA CCATCAGGGA ACTTCTCCGT GGAGGAGGCT GCCTTGATGG ATTTGACTGC 721 AGCTTCAAAC ACTTTCTTGG GCAAACGAAG GTTGGTGGTG CCACTGTCCA CAATGCTCTT 781 GTCATAGTTG TACTCCTTGC AGTCCATTTT CAGATCCTGT CCATTGATCT CCACCCGCAC 841 AATGATCACC TCATAATACC ACTCCCGCCG GATGGGTGTA TACCAGAGAC TGCCTGTGTA 901 CAGCGAGTGG TCGATACCTC CAATGATCAT GCTCCCTCCG ACAGAGGCCA GCACTTCAGA 961 CTGGGCTGAG GGGGAAGCCA GCACCACAAA GCTGCAGGGA GAAGAGGTTG GGAACGTGGG 1021 TCTGCTTTAC CAGAGAGTCA AAGAAAGGCT CCAGGGGAGT CGTCAAGGCC TGCAATCTCA 1081 GCATAGCCAG CCCCAAGATG CGTCCAGTGG AGCCGTGGAT GAGACTGTCT GAATTCAGTG 1141 ATGCAGCCAA TGGTGGCACG CCAACAAGTG AACCGCCTTG GGGCCCATTG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Kizuka, Y., Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions. Biochem. J. 473 (1): 21-30 (2016) PMID 26467158. [link to RRC of NBRP]

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human BACE1

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human BACE1

Reference sequence