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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAGGS/hBACE1 N354S

Expression vector of human BACE1mutant, N354S.

Catalog number RDB14347
Resource name pCAGGS/hBACE1 N354S
Alternative name pCAGGS/human BACE1 Asn354Ser
Clone info. Expression vector of human BACE1mutant, N354S. This Asn amino acid is N-glycosylation site of BACE1, of which there are four potential sites.
Expression was confirmed by the depositor with western blotting and immunoprecipitation.
Vector backbone pCAGGS (plasmid)
Selectable markers Amp^r
Gene/insert name human BACE1 cDNA
Depositor|Developer Taniguchi, Naoyuki | Kizuka, Yasuhiko |
 
Other clones in our bank human BACE1 (NCBI Gene 23621) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016)
Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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RDB14347 pCAGGS/hBACE1 N354S DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kizuka, Y., Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions. Biochem. J. 473 (1): 21-30 (2016) PMID 26467158.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14347_A6Itp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB14347_A6Ita.seq check
>D03881A5_A6It_2_pAxCA_F1_G10_19_ABI24.ab1
    1 GAGAACCCGG CTACCATGGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT GGGCAACGTG
   61 CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCA TGGCCCAAGC CCTGCCCTGG
  121 CTCCTGCTGT GGATGGGCGC GGGAGTGCTG CCTGCCCACG GCACCCAGCA CGGCATCCGG
  181 CTGCCCCTGC GCAGCGGCCT GGGGGGCGCC CCCCTGGGGC TGCGGCTGCC CCGGGAGACC
  241 GACGAAGAGC CCGAGGAGCC CGGCCGGAGG GGCAGCTTTG TGGAGATGGT GGACAACCTG
  301 AGGGGCAAGT CGGGGCAGGG CTACTACGTG GAGATGACCG TGGGCAGCCC CCCGCAGACG
  361 CTCAACATCC TGGTGGATAC AGGCAGCAGT AACTTTGCAG TGGGTGCTGC CCCCCACCCC
  421 TTCCTGCATC GCTACTACCA GAGGCAGCTG TCCAGCACAT ACCGGGACCT CCGGAAGGGT
  481 GTGTATGTGC CCTACACCCA GGGCAAGTGG GAAGGGGAGC TGGGCACCGA CCTGGTAAGC
  541 ATCCCCCATG GCCCCAACGT CACTGTGCGT GCCAACATTG CTGCCATCAC TGAATCAGAC
  601 AAGTTCTTCA TCAACGGCTC CAACTGGGAA GGCATCCTGG GGCTGGCCTA TGCTGAGATT
  661 GCCAGGCCTG ACGACTCCCT GGAGCCTTTC TTTGACTCTC TGGTAAAGCA GACCCACGTT
  721 CCCAACCTCT TCTCCCTGCA GCTTTGTGGT GCTGGCTTCC CCCTCAACCA GTCTGAAGTG
  781 CTGGCCTCTG TCGGAGGGAG CATGATCATT GGAGGTATCG ACCACTCGCT GTACACAGGC
  841 AGTCTCTGGT ATACACCCAT CCGGCGGGAG TGGTATTATG AGGTGATCAT TGTGCGGGTG
  901 GAGATCAATG GACAGGATCT GAAAATGGAC TGCAAGGAGT ACCACCTATG ACAAGAGCAT
  961 TGTGGGACAG TGGCAACCAC CAACCTTCGT TTTGCCCCAA GAAAGGTGTT TGGAAGCTGC
 1021 AAGTCAATCC ATTCAGGCAG CCCTCCTTCC CACGGAGAAA GTTTCCCTGA TGGTTCTGGC
 1081 CTAAGAAGAG CAGCTGATGT GCTTGGCCAA GCAGGCAACC ACCCTTGGAA CATTTTCCAA
1141 GTTCATTCCT CAAACTTCTT AACGTATGGG GTGAAGGGTT TTACTCCCAG AG
//
Primer: pCA-bgter
Sequence file: RDB14347_A6Itb.seq check
>D03881A5_A6It_2_pCA-bgter_H10_22_ABI24.ab1
    1 AGGGGTGGGA TGTGCCCAGA AAGTTTTGGC GGAGGGAGAA AGAGCTCAGT GGTATTTGTG
   61 AGCCAGGGCA TTGGCCACAC CAGCCACCAC CTTCTGATAG GCAGCCTGCA CCTGAGGAGT
  121 GAATTCTCAC TTCAGCAGGG AGATGTCATC AGCAAAGTCA TCATGCTGCT GGCGCAGGCA
  181 GCGGAGGCAG CGCCACTGAC ACACCATGAG GCAGAGTGGC AGCATGAAGA GGGCGCAGAT
  241 GGCAGCCATG ACATAGGCTA TGGTCATGAG GGTTGACTCA TCTGTCTGTG GAATGTTGTA
  301 GCCACAGTCT TCCATGTCCA AGGTGACAAA AGGGCCTTCC ACCGCTGCCG TCCTGAACTC
  361 ATCGTGCACA TGGCAAGCGC TGACAGCAAA GCCAATTCGT TTTCGGGCCC GATCAAAGAC
  421 AACGTAGAAG CCCTCCATGA TAACAGCTCC CATAACAGTG CCCGTGGATG ACTGTGAGAT
  481 GGCAAACTTG TAACAGTCGT CTTGGGACGT GGCCACATCT TCCACTGGCC GCAGGTATTG
  541 CTGCGGAAGG ATGGTGATGC GGAAGGACTG GCTGGTAACC TCACCCATTA GGTAGAGTGA
  601 GATGACTGGG AAAATGTTCC AAGGGGTGGT GCCTGCTTGC CAGCACACCA GCTGCTCTCC
  661 TAGCCAGAAA CCATCAGGGA ACTTCTCCGT GGAGGAGGCT GCCTTGATGG ATTTGACTGC
  721 AGCTTCAAAC ACTTTCTTGG GCAAACGAAG GTTGGTGGTG CCACTGTCCA CAATGCTCTT
  781 GTCATAGTTG TACTCCTTGC AGTCCATTTT CAGATCCTGT CCATTGATCT CCACCCGCAC
  841 AAATGATCAC CTCATAATAC CACTCCCGCC GGATGGGTGT ATACCAGAGA CTGCCTGTGT
  901 ACAGCGAGTG GTCGATACCT CCAATGATCA TGCTCCCTCC GACAGAGGCC AGCACTTCAG
  961 ACTGGGTTGA GGGGGGAAGC CAGCACCACA AAGCTGCAGG GAGAGGAGTT GGGGAACGTG
 1021 GGTCTGCTTT ACCAGAGAGT CAAAGAAAAG GCTCCAAGGG AGTCGTCAAG GCCTGGCAAT
 1081 CTCAGCATAG GGCCAGGCCC AGGATGCTTC CAGTGAGCCC GTTGATGGAG ACCTTGTCTG
 1141 GAATTCCATG ATGGCCAGCA TGTTGGGCAC CGCCACCAGA TGAACCGGTT GGGGGGGGCC
1201 T
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kizuka, Y., Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions. Biochem. J. 473 (1): 21-30 (2016) PMID 26467158.

2021.03.26

GNP_filter3_RDBDEP_html_210308.pl