RDB14346 - pCAGGS/hBACE1 N223S

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pCAGGS/hBACE1 N223S (#RDB14346)

Expression vector of human BACE1mutant, N223S.

Alternative name	pCAGGS/human BACE1 Asn223Ser
Clone info.	Expression vector of human BACE1mutant, N223S. This Asn amino acid is N-glycosylation site of BACE1, of which there are four potential sites.
Comment	Expression was confirmed by the depositor with western blotting and immunoprecipitation.
Vector backbone	pCAGGS (plasmid)
Selectable markers	Amp^r
Gene/insert name	human BACE1 cDNA
Depositor\|Developer	Taniguchi, Naoyuki \| Kizuka, Yasuhiko \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016)
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016) Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016)。 MTAに書く付加的使用条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016)。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14346	pCAGGS/hBACE1 N223S	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAGGS/hBACE1 N223S was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14346).

Reference section:

Kizuka, Y., Nakano, M., Kitazume, S., Saito, T., Saido, T.C., Taniguchi, N., Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions. Biochem. J. 473 (1): 21-30 (2016) PMID 26467158. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14346_A6Itp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB14346_A6Ita.seq
>D03881A4_A6It_3_pAxCA_F1_E10_13_ABI24.ab1 1 ACGTTAAACC TGCTCCATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT GGGCAACGTG 61 CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCA TGGCCCAAGC CCTGCCCTGG 121 CTCCTGCTGT GGATGGGCGC GGGAGTGCTG CCTGCCCACG GCACCCAGCA CGGCATCCGG 181 CTGCCCCTGC GCAGCGGCCT GGGGGGCGCC CCCCTGGGGC TGCGGCTGCC CCGGGAGACC 241 GACGAAGAGC CCGAGGAGCC CGGCCGGAGG GGCAGCTTTG TGGAGATGGT GGACAACCTG 301 AGGGGCAAGT CGGGGCAGGG CTACTACGTG GAGATGACCG TGGGCAGCCC CCCGCAGACG 361 CTCAACATCC TGGTGGATAC AGGCAGCAGT AACTTTGCAG TGGGTGCTGC CCCCCACCCC 421 TTCCTGCATC GCTACTACCA GAGGCAGCTG TCCAGCACAT ACCGGGACCT CCGGAAGGGT 481 GTGTATGTGC CCTACACCCA GGGCAAGTGG GAAGGGGAGC TGGGCACCGA CCTGGTAAGC 541 ATCCCCCATG GCCCCAACGT CACTGTGCGT GCCAACATTG CTGCCATCAC TGAATCAGAC 601 AAGTTCTTCA TCAACGGCTC CAACTGGGAA GGCATCCTGG GGCTGGCCTA TGCTGAGATT 661 GCCAGGCCTG ACGACTCCCT GGAGCCTTTC TTTGACTCTC TGGTAAAGCA GACCCACGTT 721 CCCAACCTCT TCTCCCTGCA GCTTTGTGGT GCTGGCTTCC CCCTCAGCCA GTCTGAAGTG 781 CTGGCCTCTG TCGGAGGGAG CATGATCATT GGAGGTATCG ACCACTCGCT GTACACAGGC 841 AGTCTCTGGT ATACACCCAT CCGGCGGGAG TGGTATTATG AGGTGATCAT TGTGCGGGTG 901 GAGATCAATG GACAGGATCT GAAAATGGAC TGCAAGGAGT ACAACTATGA CAAGAGCATT 961 GTGGACAGTG GCACCACCAA CCCTTCGTTT GCCCAAGAAA GTGTTTGAAG CTGCAGTCAA 1021 TCCATCAAGG CAGCCTCTCC ACGGAGAAGG TCCCTGATGA TTTCTGGCTA GGAGAGCAGC 1081 CTGGTGTGCT GGCCAGCAGC AACCACCCAG ACATTTTCCA GTTCATCTCA CTCTACCTTA 1141 GTGGGGGGTT GAGAGTAACA //
Primer: pCA-bgter Sequence file: RDB14346_A6Itb.seq
>D03881A4_A6It_3_pCA-bgter_F10_16_ABI24.ab1 1 AGCGATAAGT CGCAGAATTT TTGGCAGAGG GAAAAGATCT CAGTGGTATT TGTGAGCCAG 61 GGCATTGGCC ACACCAGCCA CCACCTTCTG ATAGGCAGCC TGCACCTGAG GAGTGAATTC 121 TCACTTCAGC AGGGAGATGT CATCAGCAAA GTCATCATGC TGCTGGCGCA GGCAGCGGAG 181 GCAGCGCCAC TGACACACCA TGAGGCAGAG TGGCAGCATG AAGAGGGCGC AGATGGCAGC 241 CATGACATAG GCTATGGTCA TGAGGGTTGA CTCATCTGTC TGTGGAATGT TGTAGCCACA 301 GTCTTCCATG TCCAAGGTGA CAAAAGGGCC TTCCACCGCT GCCGTCCTGA ACTCATCGTG 361 CACATGGCAA GCGCTGACAG CAAAGCCAAT TCGTTTTCGG GCCCGATCAA AGACAACGTA 421 GAAGCCCTCC ATGATAACAG CTCCCATAAC AGTGCCCGTG GATGACTGTG AGATGGCAAA 481 CTTGTAACAG TCGTCTTGGG ACGTGGCCAC ATCTTCCACT GGCCGCAGGT ATTGCTGCGG 541 AAGGATGGTG ATGCGGAAGG ACTGGTTGGT AACCTCACCC ATTAGGTAGA GTGAGATGAC 601 TGGGAAAATG TTCCAAGGGG TGGTGCCTGC TTGCCAGCAC ACCAGCTGCT CTCCTAGCCA 661 GAAACCATCA GGGAACTTCT CCGTGGAGGA GGCTGCCTTG ATGGATTTGA CTGCAGCTTC 721 AAACACTTTC TTGGGCAAAC GAAGGTTGGT GGTGCCACTG TCCACAATGC TCTTGTCATA 781 GTTGTACTCC TTGCAGTCCA TTTTCAGATC CTGTCCATTG ATCTCCACCC GCACAATGAT 841 CACCTCATAA TACCACTCCC GCCGGATGGG TGTATACCAG AGACTGCCTG TGTACAGCGA 901 GTGGTCGATA CCTCCAATGA TCATGCTCCC TCCGACAGAG GCCAGCACTT CAGACTGGCT 961 GAGGGGGAAG CCAGCACCAC AAAGCTGCAG GGAGAGAGGT TGGAACGTGG TCTGCTTTAC 1021 CAGAGAGTCA AAGAAAGCTC CAGGAGTCGT CAGCTGCATC TCAGCATAGC AGCCCCAGAA 1081 TGCGTCCCAG TGAAGCGGTG GATGAGACTG ACTGATTCAT GATGCCAGCA ATGATGCACG 1141 CACAAGGTTG ACGATTGGGG G //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Kizuka, Y., Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions. Biochem. J. 473 (1): 21-30 (2016) PMID 26467158. [link to RRC of NBRP]

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human BACE1

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human BACE1

Reference sequence