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pCAGGS/hBACE1 N223S

Expression vector of human BACE1mutant, N223S.

Catalog number RDB14346
Resource name pCAGGS/hBACE1 N223S
Alternative name pCAGGS/human BACE1 Asn223Ser
Clone info. Expression vector of human BACE1mutant, N223S. This Asn amino acid is N-glycosylation site of BACE1, of which there are four potential sites.
Expression was confirmed by the depositor with western blotting and immunoprecipitation.
Vector backbone pCAGGS (plasmid)
Selectable markers Amp^r
Gene/insert name human BACE1 cDNA
Depositor Taniguchi, Naoyuki | Kizuka, Yasuhiko |
 
Other clones in our bank human BACE1 (NCBI Gene 23621) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016)
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RDB14346 pCAGGS/hBACE1 N223S DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Kizuka, Y., Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions. Biochem. J. 473 (1): 21-30 (2016) PMID 26467158.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14346_A6Itp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB14346_A6Ita.seq
>D03881A4_A6It_3_pAxCA_F1_E10_13_ABI24.ab1
    1 ACGTTAAACC TGCTCCATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT GGGCAACGTG
   61 CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCA TGGCCCAAGC CCTGCCCTGG
  121 CTCCTGCTGT GGATGGGCGC GGGAGTGCTG CCTGCCCACG GCACCCAGCA CGGCATCCGG
  181 CTGCCCCTGC GCAGCGGCCT GGGGGGCGCC CCCCTGGGGC TGCGGCTGCC CCGGGAGACC
  241 GACGAAGAGC CCGAGGAGCC CGGCCGGAGG GGCAGCTTTG TGGAGATGGT GGACAACCTG
  301 AGGGGCAAGT CGGGGCAGGG CTACTACGTG GAGATGACCG TGGGCAGCCC CCCGCAGACG
  361 CTCAACATCC TGGTGGATAC AGGCAGCAGT AACTTTGCAG TGGGTGCTGC CCCCCACCCC
  421 TTCCTGCATC GCTACTACCA GAGGCAGCTG TCCAGCACAT ACCGGGACCT CCGGAAGGGT
  481 GTGTATGTGC CCTACACCCA GGGCAAGTGG GAAGGGGAGC TGGGCACCGA CCTGGTAAGC
  541 ATCCCCCATG GCCCCAACGT CACTGTGCGT GCCAACATTG CTGCCATCAC TGAATCAGAC
  601 AAGTTCTTCA TCAACGGCTC CAACTGGGAA GGCATCCTGG GGCTGGCCTA TGCTGAGATT
  661 GCCAGGCCTG ACGACTCCCT GGAGCCTTTC TTTGACTCTC TGGTAAAGCA GACCCACGTT
  721 CCCAACCTCT TCTCCCTGCA GCTTTGTGGT GCTGGCTTCC CCCTCAGCCA GTCTGAAGTG
  781 CTGGCCTCTG TCGGAGGGAG CATGATCATT GGAGGTATCG ACCACTCGCT GTACACAGGC
  841 AGTCTCTGGT ATACACCCAT CCGGCGGGAG TGGTATTATG AGGTGATCAT TGTGCGGGTG
  901 GAGATCAATG GACAGGATCT GAAAATGGAC TGCAAGGAGT ACAACTATGA CAAGAGCATT
  961 GTGGACAGTG GCACCACCAA CCCTTCGTTT GCCCAAGAAA GTGTTTGAAG CTGCAGTCAA
 1021 TCCATCAAGG CAGCCTCTCC ACGGAGAAGG TCCCTGATGA TTTCTGGCTA GGAGAGCAGC
 1081 CTGGTGTGCT GGCCAGCAGC AACCACCCAG ACATTTTCCA GTTCATCTCA CTCTACCTTA
1141 GTGGGGGGTT GAGAGTAACA
//
Primer: pCA-bgter RDB14346_A6Itb.seq
>D03881A4_A6It_3_pCA-bgter_F10_16_ABI24.ab1
    1 AGCGATAAGT CGCAGAATTT TTGGCAGAGG GAAAAGATCT CAGTGGTATT TGTGAGCCAG
   61 GGCATTGGCC ACACCAGCCA CCACCTTCTG ATAGGCAGCC TGCACCTGAG GAGTGAATTC
  121 TCACTTCAGC AGGGAGATGT CATCAGCAAA GTCATCATGC TGCTGGCGCA GGCAGCGGAG
  181 GCAGCGCCAC TGACACACCA TGAGGCAGAG TGGCAGCATG AAGAGGGCGC AGATGGCAGC
  241 CATGACATAG GCTATGGTCA TGAGGGTTGA CTCATCTGTC TGTGGAATGT TGTAGCCACA
  301 GTCTTCCATG TCCAAGGTGA CAAAAGGGCC TTCCACCGCT GCCGTCCTGA ACTCATCGTG
  361 CACATGGCAA GCGCTGACAG CAAAGCCAAT TCGTTTTCGG GCCCGATCAA AGACAACGTA
  421 GAAGCCCTCC ATGATAACAG CTCCCATAAC AGTGCCCGTG GATGACTGTG AGATGGCAAA
  481 CTTGTAACAG TCGTCTTGGG ACGTGGCCAC ATCTTCCACT GGCCGCAGGT ATTGCTGCGG
  541 AAGGATGGTG ATGCGGAAGG ACTGGTTGGT AACCTCACCC ATTAGGTAGA GTGAGATGAC
  601 TGGGAAAATG TTCCAAGGGG TGGTGCCTGC TTGCCAGCAC ACCAGCTGCT CTCCTAGCCA
  661 GAAACCATCA GGGAACTTCT CCGTGGAGGA GGCTGCCTTG ATGGATTTGA CTGCAGCTTC
  721 AAACACTTTC TTGGGCAAAC GAAGGTTGGT GGTGCCACTG TCCACAATGC TCTTGTCATA
  781 GTTGTACTCC TTGCAGTCCA TTTTCAGATC CTGTCCATTG ATCTCCACCC GCACAATGAT
  841 CACCTCATAA TACCACTCCC GCCGGATGGG TGTATACCAG AGACTGCCTG TGTACAGCGA
  901 GTGGTCGATA CCTCCAATGA TCATGCTCCC TCCGACAGAG GCCAGCACTT CAGACTGGCT
  961 GAGGGGGAAG CCAGCACCAC AAAGCTGCAG GGAGAGAGGT TGGAACGTGG TCTGCTTTAC
 1021 CAGAGAGTCA AAGAAAGCTC CAGGAGTCGT CAGCTGCATC TCAGCATAGC AGCCCCAGAA
 1081 TGCGTCCCAG TGAAGCGGTG GATGAGACTG ACTGATTCAT GATGCCAGCA ATGATGCACG
1141 CACAAGGTTG ACGATTGGGG G
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Kizuka, Y., Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions. Biochem. J. 473 (1): 21-30 (2016) PMID 26467158.

2018.12.11

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