RDB14345 - pCAGGS/hBACE1 N172S

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pCAGGS/hBACE1 N172S (#RDB14345)

Expression vector of human BACE1mutant, N172S.

Alternative name	pCAGGS/human BACE1 Asn172Ser
Clone info.	Expression vector of human BACE1mutant, N172S. This Asn amino acid is N-glycosylation site of BACE1, of which there are four potential sites.
Comment	Expression was confirmed by the depositor with western blotting and immunoprecipitation.
Vector backbone	pCAGGS (plasmid)
Selectable markers	Amp^r
Gene/insert name	human BACE1 cDNA
Depositor\|Developer	Taniguchi, Naoyuki \| Kizuka, Yasuhiko \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016)
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016) Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016)。 MTAに書く付加的使用条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016)。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14345	pCAGGS/hBACE1 N172S	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAGGS/hBACE1 N172S was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14345).

Reference section:

Kizuka, Y., Nakano, M., Kitazume, S., Saito, T., Saido, T.C., Taniguchi, N., Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions. Biochem. J. 473 (1): 21-30 (2016) PMID 26467158. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14345_A6Itp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB14345_A6Ita.seq
>D03881A3_A6It_2_pAxCA_F1_C11_03_ABI08.ab1 1 CTTCCCGGCC TACCATGTTC ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG GCAACGTGCT 61 GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCATG GCCCAAGCCC TGCCCTGGCT 121 CCTGCTGTGG ATGGGCGCGG GAGTGCTGCC TGCCCACGGC ACCCAGCACG GCATCCGGCT 181 GCCCCTGCGC AGCGGCCTGG GGGGCGCCCC CCTGGGGCTG CGGCTGCCCC GGGAGACCGA 241 CGAAGAGCCC GAGGAGCCCG GCCGGAGGGG CAGCTTTGTG GAGATGGTGG ACAACCTGAG 301 GGGCAAGTCG GGGCAGGGCT ACTACGTGGA GATGACCGTG GGCAGCCCCC CGCAGACGCT 361 CAACATCCTG GTGGATACAG GCAGCAGTAA CTTTGCAGTG GGTGCTGCCC CCCACCCCTT 421 CCTGCATCGC TACTACCAGA GGCAGCTGTC CAGCACATAC CGGGACCTCC GGAAGGGTGT 481 GTATGTGCCC TACACCCAGG GCAAGTGGGA AGGGGAGCTG GGCACCGACC TGGTAAGCAT 541 CCCCCATGGC CCCAACGTCA CTGTGCGTGC CAACATTGCT GCCATCACTG AATCAGACAA 601 GTTCTTCATC AGCGGCTCCA ACTGGGAAGG CATCCTGGGG CTGGCCTATG CTGAGATTGC 661 CAGGCCTGAC GACTCCCTGG AGCCTTTCTT TGACTCTCTG GTAAAGCAGA CCCACGTTCC 721 CAACCTCTTC TCCCTGCAGC TTTGTGGTGC TGGCTTCCCC CTCAACCAGT CTGAAGTGCT 781 GGCCTCTGTC GGAGGGAGCA TGATCATTGG AGGTATCGAC CACTCGCTGT ACACAGGCAG 841 TCTCTGGTAT ACACCCATCC GGCGGGAGTG GTATTATGAG GTGATCATTG TGCGGGTGGA 901 GATCAATGGA CAGGATCTGA AAATGGACTG CAAGGAGTAC AACTATGACA GAGCATTGTG 961 GACAGTGGCA CCACCAACCC TTCGTTTGCC CAGAAAGTGT TTGAAGCTGC AGTCAATCCC 1021 ATCAAGGCAG CCTCCTCCAC GGAGAGTCCC TGATGTTTTC TGGCTAGGAG AGCCAGCCTG 1081 GTGTGCTGCA GCCAAGGCAC CACCCCTGAC ATTACAGTCA TCTCACTCTA CATATGAGTA 1141 GGTACCAAAT CA //
Primer: pCA-bgter Sequence file: RDB14345_A6Itb.seq
>D03881A3_A6It_2_pCA-bgter_D10_10_ABI24.ab1 1 AGCCGCATGA AGGTGCCCAG AAATTTTTGG CAGAGGGAAA AAGATCTCAG TGGTATTTGT 61 GAGCCAGGGC ATTGGCCACA CCAGCCACCA CCTTCTGATA GGCAGCCTGC ACCTGAGGAG 121 TGAATTCTCA CTTCAGCAGG GAGATGTCAT CAGCAAAGTC ATCATGCTGC TGGCGCAGGC 181 AGCGGAGGCA GCGCCACTGA CACACCATGA GGCAGAGTGG CAGCATGAAG AGGGCGCAGA 241 TGGCAGCCAT GACATAGGCT ATGGTCATGA GGGTTGACTC ATCTGTCTGT GGAATGTTGT 301 AGCCACAGTC TTCCATGTCC AAGGTGACAA AAGGGCCTTC CACCGCTGCC GTCCTGAACT 361 CATCGTGCAC ATGGCAAGCG CTGACAGCAA AGCCAATTCG TTTTCGGGCC CGATCAAAGA 421 CAACGTAGAA GCCCTCCATG ATAACAGCTC CCATAACAGT GCCCGTGGAT GACTGTGAGA 481 TGGCAAACTT GTAACAGTCG TCTTGGGACG TGGCCACATC TTCCACTGGC CGCAGGTATT 541 GCTGCGGAAG GATGGTGATG CGGAAGGACT GGTTGGTAAC CTCACCCATT AGGTAGAGTG 601 AGATGACTGG GAAAATGTTC CAAGGGGTGG TGCCTGCTTG CCAGCACACC AGCTGCTCTC 661 CTAGCCAGAA ACCATCAGGG AACTTCTCCG TGGAGGAGGC TGCCTTGATG GATTTGACTG 721 CAGCTTCAAA CACTTTCTTG GGCAAACGAA GGTTGGTGGT GCCACTGTCC ACAATGCTCT 781 TGTCATAGTT GTACTCCTTG CAGTCCATTT TCAGATCCTG TCCATTGATC TCCACCCGCA 841 CAATGATCAC CTCATAATAC CACTCCCGCC GGATGGGTGT ATACCAGAGA CTGCCTGTGT 901 ACAGCGAGTG GGTCGATACC TCCAATGATC ATGCTCCCTC CGACAGAGGC CAGCACTTCA 961 GACTGGTTGA GGGGGAAGCC AGCACCACAA AGCTGCAGGG AGGAGAGGTT GGGAACGTGG 1021 GTCTGCTTTA CCAGAGAGTC AAAAGAAAGG CTTCAGGAGT CGTCAGGCCT GGCAATCTTC 1081 AGCATAGGCC AGCCCCAGAT GCTTTCCAGT TTGGGAGCCG GCTGATGAGA CTTGGTCTGG 1141 ATTTCATGAT GCCAGCCAAT GTTTGCCACC GCCACCAGAG GTGGAACGCG CTTG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Kizuka, Y., Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions. Biochem. J. 473 (1): 21-30 (2016) PMID 26467158. [link to RRC of NBRP]

Resource search

Link

Other resources in our bank

human BACE1

External Database
human BACE1

Reference sequence