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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAGGS/hBACE1 N172S

Expression vector of human BACE1mutant, N172S.

Catalog number RDB14345
Resource name pCAGGS/hBACE1 N172S
Alternative name pCAGGS/human BACE1 Asn172Ser
Clone info. Expression vector of human BACE1mutant, N172S. This Asn amino acid is N-glycosylation site of BACE1, of which there are four potential sites.
Expression was confirmed by the depositor with western blotting and immunoprecipitation.
Vector backbone pCAGGS (plasmid)
Selectable markers Amp^r
Gene/insert name human BACE1 cDNA
Depositor|Developer Taniguchi, Naoyuki | Kizuka, Yasuhiko |
 
Other clones in our bank human BACE1 (NCBI Gene 23621) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016)
Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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RDB14345 pCAGGS/hBACE1 N172S DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kizuka, Y., Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions. Biochem. J. 473 (1): 21-30 (2016) PMID 26467158.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14345_A6Itp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB14345_A6Ita.seq check
>D03881A3_A6It_2_pAxCA_F1_C11_03_ABI08.ab1
    1 CTTCCCGGCC TACCATGTTC ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG GCAACGTGCT
   61 GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCATG GCCCAAGCCC TGCCCTGGCT
  121 CCTGCTGTGG ATGGGCGCGG GAGTGCTGCC TGCCCACGGC ACCCAGCACG GCATCCGGCT
  181 GCCCCTGCGC AGCGGCCTGG GGGGCGCCCC CCTGGGGCTG CGGCTGCCCC GGGAGACCGA
  241 CGAAGAGCCC GAGGAGCCCG GCCGGAGGGG CAGCTTTGTG GAGATGGTGG ACAACCTGAG
  301 GGGCAAGTCG GGGCAGGGCT ACTACGTGGA GATGACCGTG GGCAGCCCCC CGCAGACGCT
  361 CAACATCCTG GTGGATACAG GCAGCAGTAA CTTTGCAGTG GGTGCTGCCC CCCACCCCTT
  421 CCTGCATCGC TACTACCAGA GGCAGCTGTC CAGCACATAC CGGGACCTCC GGAAGGGTGT
  481 GTATGTGCCC TACACCCAGG GCAAGTGGGA AGGGGAGCTG GGCACCGACC TGGTAAGCAT
  541 CCCCCATGGC CCCAACGTCA CTGTGCGTGC CAACATTGCT GCCATCACTG AATCAGACAA
  601 GTTCTTCATC AGCGGCTCCA ACTGGGAAGG CATCCTGGGG CTGGCCTATG CTGAGATTGC
  661 CAGGCCTGAC GACTCCCTGG AGCCTTTCTT TGACTCTCTG GTAAAGCAGA CCCACGTTCC
  721 CAACCTCTTC TCCCTGCAGC TTTGTGGTGC TGGCTTCCCC CTCAACCAGT CTGAAGTGCT
  781 GGCCTCTGTC GGAGGGAGCA TGATCATTGG AGGTATCGAC CACTCGCTGT ACACAGGCAG
  841 TCTCTGGTAT ACACCCATCC GGCGGGAGTG GTATTATGAG GTGATCATTG TGCGGGTGGA
  901 GATCAATGGA CAGGATCTGA AAATGGACTG CAAGGAGTAC AACTATGACA GAGCATTGTG
  961 GACAGTGGCA CCACCAACCC TTCGTTTGCC CAGAAAGTGT TTGAAGCTGC AGTCAATCCC
 1021 ATCAAGGCAG CCTCCTCCAC GGAGAGTCCC TGATGTTTTC TGGCTAGGAG AGCCAGCCTG
 1081 GTGTGCTGCA GCCAAGGCAC CACCCCTGAC ATTACAGTCA TCTCACTCTA CATATGAGTA
1141 GGTACCAAAT CA
//
Primer: pCA-bgter
Sequence file: RDB14345_A6Itb.seq check
>D03881A3_A6It_2_pCA-bgter_D10_10_ABI24.ab1
    1 AGCCGCATGA AGGTGCCCAG AAATTTTTGG CAGAGGGAAA AAGATCTCAG TGGTATTTGT
   61 GAGCCAGGGC ATTGGCCACA CCAGCCACCA CCTTCTGATA GGCAGCCTGC ACCTGAGGAG
  121 TGAATTCTCA CTTCAGCAGG GAGATGTCAT CAGCAAAGTC ATCATGCTGC TGGCGCAGGC
  181 AGCGGAGGCA GCGCCACTGA CACACCATGA GGCAGAGTGG CAGCATGAAG AGGGCGCAGA
  241 TGGCAGCCAT GACATAGGCT ATGGTCATGA GGGTTGACTC ATCTGTCTGT GGAATGTTGT
  301 AGCCACAGTC TTCCATGTCC AAGGTGACAA AAGGGCCTTC CACCGCTGCC GTCCTGAACT
  361 CATCGTGCAC ATGGCAAGCG CTGACAGCAA AGCCAATTCG TTTTCGGGCC CGATCAAAGA
  421 CAACGTAGAA GCCCTCCATG ATAACAGCTC CCATAACAGT GCCCGTGGAT GACTGTGAGA
  481 TGGCAAACTT GTAACAGTCG TCTTGGGACG TGGCCACATC TTCCACTGGC CGCAGGTATT
  541 GCTGCGGAAG GATGGTGATG CGGAAGGACT GGTTGGTAAC CTCACCCATT AGGTAGAGTG
  601 AGATGACTGG GAAAATGTTC CAAGGGGTGG TGCCTGCTTG CCAGCACACC AGCTGCTCTC
  661 CTAGCCAGAA ACCATCAGGG AACTTCTCCG TGGAGGAGGC TGCCTTGATG GATTTGACTG
  721 CAGCTTCAAA CACTTTCTTG GGCAAACGAA GGTTGGTGGT GCCACTGTCC ACAATGCTCT
  781 TGTCATAGTT GTACTCCTTG CAGTCCATTT TCAGATCCTG TCCATTGATC TCCACCCGCA
  841 CAATGATCAC CTCATAATAC CACTCCCGCC GGATGGGTGT ATACCAGAGA CTGCCTGTGT
  901 ACAGCGAGTG GGTCGATACC TCCAATGATC ATGCTCCCTC CGACAGAGGC CAGCACTTCA
  961 GACTGGTTGA GGGGGAAGCC AGCACCACAA AGCTGCAGGG AGGAGAGGTT GGGAACGTGG
 1021 GTCTGCTTTA CCAGAGAGTC AAAAGAAAGG CTTCAGGAGT CGTCAGGCCT GGCAATCTTC
 1081 AGCATAGGCC AGCCCCAGAT GCTTTCCAGT TTGGGAGCCG GCTGATGAGA CTTGGTCTGG
1141 ATTTCATGAT GCCAGCCAAT GTTTGCCACC GCCACCAGAG GTGGAACGCG CTTG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kizuka, Y., Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions. Biochem. J. 473 (1): 21-30 (2016) PMID 26467158.

2021.03.26

GNP_filter3_RDBDEP_html_210308.pl