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pEF-HA-RACK1

Expression vector of HA-tagged mouse receptor of activated protein kinase C1 (RACK1) full-length (1-317 aa).

Catalog number RDB14335
Resource name pEF-HA-RACK1
Clone info. Expression vector driven by the EF1 promoter for HA-tagged mouse receptor of activated protein kinase C1 (RACK1) full-length (1-317 aa), see Fig. 1b of Marubashi, S. et al. J. Invest. Dermatol., 136 (8): 1672-1680, 2016.
Expression was confirmed by the depositor with immunoprecipitation.
Vector backbone pEF-HA (Plasmid)
Selectable markers Amp^r
Gene/insert name mouse RACK1 cDNA
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse RACK1 (NCBI Gene 14694) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Marubashi, S. et al., J. Invest. Dermatol., 136 (8): 1672-1680, 2016)
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14335 pEF-HA-RACK1 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Marubashi, S., A Varp-Binding Protein, RACK1, Regulates Dendrite Outgrowth through Stabilization of Varp Protein in Mouse Melanocytes. J. Invest. Dermatol. 136 (8): 1672-1680 (2016). PMID 27066885.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14335_A6I8p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F RDB14335_A6I8a.seq
>D03786A3_A6I8_1_Efla-C_F_E07_13_ABI24.ab1
    1 TCGTGAGTGC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA
   61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGCGGCC GCGGGAATTC
  121 GATTCCACCA TGGGTTACCC ATACGACGTC CCAGACTACG CTGGATCCAT GACCGAGCAG
  181 ATGACCCTTC GCGGGACCCT TAAGGGCCAC AATGGATGGG TAACACAGAT CGCCACCACA
  241 CCGCAGTTCC CGGACATGAT CCTGTCTGCG TCTCGAGACA AGACCATCAT CATGTGGAAG
  301 CTGACCAGAG ATGAGACCAA CTATGGCATA CCACAGCGTG CTCTGAGAGG TCACTCCCAC
  361 TTCGTTAGTG ATGTTGTTAT CTCCTCTGAT GGTCAGTTTG CCCTCTCGGG CTCCTGGGAC
  421 GGAACGCTGC GCCTCTGGGA TCTCACAACG GGCACCACCA CAAGGCGATT TGTCGGCCAC
  481 ACCAAGGATG TGTTGAGCGT GGCCTTCTCC TCTGACAACC GGCAGATTGT CTCTGGGTCC
  541 CGAGACAAGA CCATAAAGTT ATGGAATACT CTGGGTGTCT GCAAGTACAC GGTCCAGGAT
  601 GAGAGTCATT CAGAATGGGT GTCTTGTGTC CGCTTCTCCC CGAACAGCAG CAACCCTATC
  661 ATCGTCTCCT GCGGATGGGA CAAGCTGGTC AAGGTGTGGA ATCTGGCTAA CTGCAAGCTA
  721 AAGACCAACC ACATTGGCCA CACTGGCTAC CTGAACACAG TGACTGTCTC TCCAGATGGA
  781 TCCCTCTGTG CTTCTGGAGG CAAGGATGGC CAGGCTATGC TGTGGGATCT CAATGAAGGC
  841 AAGCACCTCT ACACTTTAGA TGGTGGGGAC ATCATCAATG CCTTGTGCTT CAGCCCCAAC
  901 CGCTACTGGC TCTGCGCTGC CACTGGCCCC AGCATCAAGA TCTGGGACTT GGGAGGGCAA
  961 GATCATTGTA GATGAATTGA AGCAGAAGTT ATCAGCAACA GCAGCAGCAG AGCCACCCCC
 1021 AGTGTACTCT TGGCATGGTC TGCTGAATGC AGACTCTGGT TTGCCTTGCT ATACAGACAG
1081 ACTTCGATGC GAGCTATGCC AAGGCTTACC TAATTGGTA
//
Primer: hGCSF-R RDB14335_A6I8b.seq
>D03786A3_A6I8_1_hGCSF_R_F07_16_ABI24.ab1
    1 CTGGGGACTG ATCTCTCAGG GTCCCAGGTG GGGACCCTCA CTCTAGAGCG GCCGCTATGG
   61 TCGACCTGCA GGCGGCCGCG AATTCACTAG TGATTTTAGC GGGTACCAAT AGTTACCTGC
  121 CATACTCGCA CCAAGTTGTC TGTATAGCCA GCAAACAGAG TCTGGCCATC AGCAGACCAT
  181 GCCAAAGAGG TACACTGGGG TGGCTCTGCC TTGCTGCTGG TGCTGATAAC TTCTTGCTTC
  241 AATTCATCTA CAATGATCTT GCCCTCCAAG TCCCAGATCT TGATGCTGGG GCCAGTGGCA
  301 GCGCAGAGCC AGTAGCGGTT GGGGCTGAAG CACAAGGCAT TGATGATGTC CCCACCATCT
  361 AAAGTGTAGA GGTGCTTGCC TTCATTGAGA TCCCACAGCA TAGCCTGGCC ATCCTTGCCT
  421 CCAGAAGCAC AGAGGGATCC ATCTGGAGAG ACAGTCACTG TGTTCAGGTA GCCAGTGTGG
  481 CCAATGTGGT TGGTCTTTAG CTTGCAGTTA GCCAGATTCC ACACCTTGAC CAGCTTGTCC
  541 CATCCGCAGG AGACGATGAT AGGGTTGCTG CTGTTCGGGG AGAAGCGGAC ACAAGACACC
  601 CATTCTGAAT GACTCTCATC CTGGACCGTG TACTTGCAGA CACCCAGAGT ATTCCATAAC
  661 TTTATGGTCT TGTCTCGGGA CCCAGAGACA ATCTGCCGGT TGTCAGAGGA GAAGGCCACG
  721 CTCAACACAT CCTTGGTGTG GCCGACAAAT CGCCTTGTGG TGGTGCCCGT TGTGAGATCC
  781 CAGAGGCGCA GCGTTCCGTC CCAGGAGCCC GAGAGGGCAA ACTGACCATC AGAGGAGATA
  841 ACAACATCAC TAACGAAGTG GGAGTGACCT CTCAGAGCAC GCTGTGGTAT GCCATAGTTG
  901 GTCTCATCTC TGGTCAGCTT CCACATGATG ATGGTCTTGT CTCGAGACGC AGACAGGATC
  961 ATGTCCGGGA ACTGCGGGTG TGGGTGGCGA TCTGTGTTAC CCATCCATTT GTGGGCCCTT
 1021 TAGGGTCCCG CGAAGGGTCA TCTGCTCGGT CATGGGAATC AGCGTTAGTC TGGGGACGTC
 1081 GTAATGGTAC TCATGATTGG TAATTTCGGA CTTCCCCCGC CGGATCGCCC TCTCTTAGAG
1141 A
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Marubashi, S., A Varp-Binding Protein, RACK1, Regulates Dendrite Outgrowth through Stabilization of Varp Protein in Mouse Melanocytes. J. Invest. Dermatol. 136 (8): 1672-1680 (2016). PMID 27066885.

2019.03.15

GNP_filter3_RDBDEP_html_190307.pl