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pEF-HA-RACK1 (#RDB14335)

Expression vector of HA-tagged mouse receptor of activated protein kinase C1 (RACK1) full-length (1-317 aa).

Clone info. Expression vector driven by the EF1 promoter for HA-tagged mouse receptor of activated protein kinase C1 (RACK1) full-length (1-317 aa), see Fig. 1b of Marubashi, S. et al. J. Invest. Dermatol., 136 (8): 1672-1680, 2016.
Comment Expression was confirmed by the depositor with immunoprecipitation.
Vector backbone pEF-HA (Plasmid)
Selectable markers Amp^r
Gene/insert name mouse RACK1 cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Marubashi, S. et al., J. Invest. Dermatol., 136 (8): 1672-1680, 2016)
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Marubashi, S. et al., J. Invest. Dermatol., 136 (8): 1672-1680, 2016)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14335 pEF-HA-RACK1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-HA-RACK1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14335).

Reference section:

Marubashi, S., Ohbayashi, N., Fukuda, M., A Varp-Binding Protein, RACK1, Regulates Dendrite Outgrowth through Stabilization of Varp Protein in Mouse Melanocytes. J. Invest. Dermatol. 136 (8): 1672-1680 (2016). PMID 27066885. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14335_A6I8p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F ()
Sequence file: RDB14335_A6I8a.seq check
>D03786A3_A6I8_1_Efla-C_F_E07_13_ABI24.ab1
    1 TCGTGAGTGC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA
   61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGCGGCC GCGGGAATTC
  121 GATTCCACCA TGGGTTACCC ATACGACGTC CCAGACTACG CTGGATCCAT GACCGAGCAG
  181 ATGACCCTTC GCGGGACCCT TAAGGGCCAC AATGGATGGG TAACACAGAT CGCCACCACA
  241 CCGCAGTTCC CGGACATGAT CCTGTCTGCG TCTCGAGACA AGACCATCAT CATGTGGAAG
  301 CTGACCAGAG ATGAGACCAA CTATGGCATA CCACAGCGTG CTCTGAGAGG TCACTCCCAC
  361 TTCGTTAGTG ATGTTGTTAT CTCCTCTGAT GGTCAGTTTG CCCTCTCGGG CTCCTGGGAC
  421 GGAACGCTGC GCCTCTGGGA TCTCACAACG GGCACCACCA CAAGGCGATT TGTCGGCCAC
  481 ACCAAGGATG TGTTGAGCGT GGCCTTCTCC TCTGACAACC GGCAGATTGT CTCTGGGTCC
  541 CGAGACAAGA CCATAAAGTT ATGGAATACT CTGGGTGTCT GCAAGTACAC GGTCCAGGAT
  601 GAGAGTCATT CAGAATGGGT GTCTTGTGTC CGCTTCTCCC CGAACAGCAG CAACCCTATC
  661 ATCGTCTCCT GCGGATGGGA CAAGCTGGTC AAGGTGTGGA ATCTGGCTAA CTGCAAGCTA
  721 AAGACCAACC ACATTGGCCA CACTGGCTAC CTGAACACAG TGACTGTCTC TCCAGATGGA
  781 TCCCTCTGTG CTTCTGGAGG CAAGGATGGC CAGGCTATGC TGTGGGATCT CAATGAAGGC
  841 AAGCACCTCT ACACTTTAGA TGGTGGGGAC ATCATCAATG CCTTGTGCTT CAGCCCCAAC
  901 CGCTACTGGC TCTGCGCTGC CACTGGCCCC AGCATCAAGA TCTGGGACTT GGGAGGGCAA
  961 GATCATTGTA GATGAATTGA AGCAGAAGTT ATCAGCAACA GCAGCAGCAG AGCCACCCCC
 1021 AGTGTACTCT TGGCATGGTC TGCTGAATGC AGACTCTGGT TTGCCTTGCT ATACAGACAG
1081 ACTTCGATGC GAGCTATGCC AAGGCTTACC TAATTGGTA
//
Primer: hGCSF-R ()
Sequence file: RDB14335_A6I8b.seq check
>D03786A3_A6I8_1_hGCSF_R_F07_16_ABI24.ab1
    1 CTGGGGACTG ATCTCTCAGG GTCCCAGGTG GGGACCCTCA CTCTAGAGCG GCCGCTATGG
   61 TCGACCTGCA GGCGGCCGCG AATTCACTAG TGATTTTAGC GGGTACCAAT AGTTACCTGC
  121 CATACTCGCA CCAAGTTGTC TGTATAGCCA GCAAACAGAG TCTGGCCATC AGCAGACCAT
  181 GCCAAAGAGG TACACTGGGG TGGCTCTGCC TTGCTGCTGG TGCTGATAAC TTCTTGCTTC
  241 AATTCATCTA CAATGATCTT GCCCTCCAAG TCCCAGATCT TGATGCTGGG GCCAGTGGCA
  301 GCGCAGAGCC AGTAGCGGTT GGGGCTGAAG CACAAGGCAT TGATGATGTC CCCACCATCT
  361 AAAGTGTAGA GGTGCTTGCC TTCATTGAGA TCCCACAGCA TAGCCTGGCC ATCCTTGCCT
  421 CCAGAAGCAC AGAGGGATCC ATCTGGAGAG ACAGTCACTG TGTTCAGGTA GCCAGTGTGG
  481 CCAATGTGGT TGGTCTTTAG CTTGCAGTTA GCCAGATTCC ACACCTTGAC CAGCTTGTCC
  541 CATCCGCAGG AGACGATGAT AGGGTTGCTG CTGTTCGGGG AGAAGCGGAC ACAAGACACC
  601 CATTCTGAAT GACTCTCATC CTGGACCGTG TACTTGCAGA CACCCAGAGT ATTCCATAAC
  661 TTTATGGTCT TGTCTCGGGA CCCAGAGACA ATCTGCCGGT TGTCAGAGGA GAAGGCCACG
  721 CTCAACACAT CCTTGGTGTG GCCGACAAAT CGCCTTGTGG TGGTGCCCGT TGTGAGATCC
  781 CAGAGGCGCA GCGTTCCGTC CCAGGAGCCC GAGAGGGCAA ACTGACCATC AGAGGAGATA
  841 ACAACATCAC TAACGAAGTG GGAGTGACCT CTCAGAGCAC GCTGTGGTAT GCCATAGTTG
  901 GTCTCATCTC TGGTCAGCTT CCACATGATG ATGGTCTTGT CTCGAGACGC AGACAGGATC
  961 ATGTCCGGGA ACTGCGGGTG TGGGTGGCGA TCTGTGTTAC CCATCCATTT GTGGGCCCTT
 1021 TAGGGTCCCG CGAAGGGTCA TCTGCTCGGT CATGGGAATC AGCGTTAGTC TGGGGACGTC
 1081 GTAATGGTAC TCATGATTGG TAATTTCGGA CTTCCCCCGC CGGATCGCCC TCTCTTAGAG
1141 A
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Marubashi, S., A Varp-Binding Protein, RACK1, Regulates Dendrite Outgrowth through Stabilization of Varp Protein in Mouse Melanocytes. J. Invest. Dermatol. 136 (8): 1672-1680 (2016). PMID 27066885. [link to RRC of NBRP]