Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCXN2-FLAG-hTBK1(ULD+CC)

Expression vector of FLAG tagged human TBK1, deletion mutant of kinase domain (KD) (1-304 AA).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Yamada, T. et al., Nat. Immunol. 17 (6): 687-694, 2016)
Additional terms and conditions for distribution	Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yamada, T. et al., Nat. Immunol. 17 (6): 687-694, 2016)。
付加的提供条件	CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14332_A6I5p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB14332_A6I5a.seq
>D03656B1_A6I5_1_pAxCA_F1_A06_03_ABI24.ab1     1 GAGGGGGGGT TTGCTCATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT GGGCAACGTG    61 CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCA AGCTTAGCCA CCATGGACTA   121 CAAAGACGAT GACGATAAAG CAAGGCTCGA GATACTTCAC CGAATGGTAA TTCATGTTTT   181 TTCGCTACAA CAAATGACAG CTCATAAGAT TTATATACAT AGCTATAATA CTGCTACTAT   241 ATTTCATGAA CTGGTATATA AACAAACCAA AATTATTTCT TCAAATCAAG AACTTATCTA   301 CGAAGGGCGA CGCTTAGTCT TAGAACCTGG AAGGCTGGCA CAACATTTCC CTAAAACTAC   361 TGAGGAAAAC CCTATATTTG TAGTAAGCCG GGAACCTCTG AATACCATAG GATTAATATA   421 TGAAAAAATT TCCCTCCCTA AAGTACATCC ACGTTATGAT TTAGACGGGG ATGCTAGCAT   481 GGCTAAGGCA ATAACAGGGG TTGTGTGTTA TGCCTGCAGA ATTGCCAGTA CCTTACTGCT   541 TTATCAGGAA TTAATGCGAA AGGGGATACG ATGGCTGATT GAATTAATTA AAGATGATTA   601 CAATGAAACT GTTCACAAAA AGACAGAAGT TGTGATCACA TTGGATTTCT GTATCAGAAA   661 CATTGAAAAA ACTGTGAAAG TATATGAAAA GTTGATGAAG ATCAACCTGG AAGCGGCAGA   721 GTTAGGTGAA ATTTCAGACA TACACACCAA ATTGTTGAGA CTTTCCAGTT CTCAGGGAAC   781 AATAGAAACC AGTCTTCAGG ATATCGACAG CAGATTATCT CCAGGTGGAT CACTGGCAGA   841 CGCATGGGCA CATCAAGAAG GCACTCATCC GAAAGACAGA AATGTAGAAA AACTACAAGT   901 CCTGTTAAAT TGCATGACAG AGATTTACTA TCAGTTCAAA AAAGACAAAG CAGAACGTAG   961 ATTAGCTTAT AATGAAGAAC AAATCCCACA AATTTGATAA GCAAAAACTG TATTACCATG  1021 CCACAAAAGC TATGACGCAC TTTACAGATG AATGTGTTAA AAAGTATGAG CATTTTTTGA  1081 ATAAGTCAGG AAGATGTATA AGAAGGATGC CTCAATCTTA GCAAACAGGT TTATTTATTC 1141 G //
Primer: pAxCA_R1 Sequence file: RDB14332_A6I5b.seq
>D03656B1_A6I5_1_pAxCA_R1_B06_06_ABI24.ab1     1 TTTGAACAGG GCATTGGCCA CACCAGCCAC CACCTTCTGA TAGGCAGCCT GCACCTGAGG    61 AGTGAATTAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC   121 GCGCAATTAA CCCTCACTAA AGGGAACAAA AGCTGGAGCT CCACCGCGGT GGCGGCCGCC   181 TAAAGACAGT CAACGTTGCG AAGGCCACCA TCCATGGTTA AAGAGCCAAA CCTTTCTAAA   241 ATGTGGTTAT TTTCAGCAAG TTCTTTAACC ACCCCTTCCA TCTCTTCCTT TAATTTCTTC   301 ATACCAAGAG TCATTTCTAC TAATGTGTTA GAACTTGGAT AAATTGGGGT CATGGTATGT   361 TTGATTCCAC TGGAAGCTGT AAACATTTTC TGAGGCAGAG TTTCTTGTAA CTCATTAGTA   421 TATTCTTGAT ATTTTGATAC TTCTTCTTCA ATATCAAAAC ACTGATTAGT CAGCGATAAT   481 AACTGTTTCC TAAGATGAAG CATCTTTCTT ATCCATTCTT CTGACTTATT CAAAAATGCC   541 TCATACTTTT TAACACATTC ATCTGTAAAG TGCGTCATAG CTTTTGTGGC ATGGTAATAC   601 AGTTTTTGCT TATCAAATTT GTGGATTTGT TCTTCATTAT AAGCTAATCT ACGTTCTGCT   661 TTGTCTTTTT TGAACTGATA GTAAATCTCT GTCATGCAAT TTAACAGGAC TTGTAGTTTT   721 TCTACATTTC TGTCTTTCGG ATGAGTGCCT TCTTGATGTG CCCATGCGTC TGCCAGTGAT   781 CCACCTGGAG ATAATCTGCT GTCGATATCC TGAAGACTGG TTTCTATTGT TCCCTGAGAA   841 CTGGAAAGTC TCAACAATTT GGTGTGTATG TCTGAAATTT CACCTAACTC TGCCGCTTCC   901 AGGTTGATCT TCATCAACTT TTCATATACT TTCACAGTTT TTTTCAATGT TTCTGATACA   961 GAAATCCAAT GTGATCACAA ACTTCTGTCT TTTTGTGAAC AGTTTCATTG TAAATCATCT  1021 TTAATTTAAT TCAATCAGCC ATCGTATCCC CTTTCGCAAT TAAATTCCCT TGATAAAGCA  1081 AGTAAGTACT GGTAACTTCT GCAGGCATTA ACCACACACC CCCATATTAT TGGCCTTAAG 1141 GCCAATGGCC TTAGG //
Primer: Amp_R Sequence file: RDB14332_A6I5c.seq
>D03656B1_A6I5_1_Amp_R_E06_15_ABI24.ab1     1 CTTTGTTTCC ATTATTTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGGATACA    61 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG   121 TGCCACCTGG GTCGAGCAGT GTGGTTTTCA AGAGGAAGCA AAAAGCCTCT CCACCCAGGC   181 CTGGAATGTT TCCACCCAAT GTCGAGCAGT GTGGTTTTGC AAGAGGAAGC AAAAAGCCTC   241 TCCACCCAGG CCTGGAATGT TTCCACCCAA TGTCGAGCAA ACCCCGCCCA GCGTCTTGTC   301 ATTGGCGAAT TAATTCGAAC ACGCAGATGC AGTCGGGGCG GCGCGGTCCC AGGTCCACTT   361 CGCATATTAA GGTGACGCGT GTGGCCTCGA ACACCGAGCG ACCCTGCAGC CAATATGGGA   421 TCGGCCATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA   481 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG   541 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA   601 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT   661 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG   721 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA   781 ATGCGGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAAGCGAAAC   841 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAAGCCGGTC TTGTCGATCA GGATGATCTG   901 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG   961 CCCGACGGGC GATGATCTCG TCGTGACCCA TGGCCGATGC CCTGCTTTGC CCGAATATCA  1021 TGGTGGAAAT GGGCCCGCTT TTTCTGGAAT TTCAATCGAC TGTGGGCCCG GCTGGTGTGC  1081 CGGACCGCCT TATTCAGGAC ATAAGCCGTT GGCTAACCGT GAATATTGCT GGAAGAGACC 1141 TTGGTCGGGC GAAAATGAGG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl