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pCXN2-FLAG-hTBK1(ULD+CC)

Expression vector of FLAG tagged human TBK1, deletion mutant of kinase domain (KD) (1-304 AA).

Catalog number RDB14332
Resource name pCXN2-FLAG-hTBK1(ULD+CC)
Clone info. Expression vector driven by the CAG promoter for FLAG tagged human TANK binding kinase 1 (TBK1) mutant; deletion of kinase domain (KD) (1-304 AA). See Fig. S4d of Yamada, T., Nat. Immunol, 17 (6): 687-694, 2016 for details.
Expression was confirmed by the deposotor with immunoprecipitation and immunoblotting.
Vector backbone pCXN2-Flag (Plasmid)
Selectable markers Amp^r
Gene/insert name human TBK1 cDNA
Depositor Takaoka, Akinori |
 
Other clones in our bank human TBK1 (NCBI Gene 29110) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Yamada, T. et al., Nat. Immunol. 17 (6): 687-694, 2016)
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RDB14332 pCXN2-FLAG-hTBK1(ULD+CC) DNA solution

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References and tips

Electronic file

Featured content

References

original Yamada, T., Constitutive aryl hydrocarbon receptor signaling constrains type I interferon-mediated antiviral innate defense. Nat. Immunol. 17 (6): 687-694 (2016). PMID 27089381.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14332_A6I5p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB14332_A6I5a.seq
>D03656B1_A6I5_1_pAxCA_F1_A06_03_ABI24.ab1
    1 GAGGGGGGGT TTGCTCATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT GGGCAACGTG
   61 CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCA AGCTTAGCCA CCATGGACTA
  121 CAAAGACGAT GACGATAAAG CAAGGCTCGA GATACTTCAC CGAATGGTAA TTCATGTTTT
  181 TTCGCTACAA CAAATGACAG CTCATAAGAT TTATATACAT AGCTATAATA CTGCTACTAT
  241 ATTTCATGAA CTGGTATATA AACAAACCAA AATTATTTCT TCAAATCAAG AACTTATCTA
  301 CGAAGGGCGA CGCTTAGTCT TAGAACCTGG AAGGCTGGCA CAACATTTCC CTAAAACTAC
  361 TGAGGAAAAC CCTATATTTG TAGTAAGCCG GGAACCTCTG AATACCATAG GATTAATATA
  421 TGAAAAAATT TCCCTCCCTA AAGTACATCC ACGTTATGAT TTAGACGGGG ATGCTAGCAT
  481 GGCTAAGGCA ATAACAGGGG TTGTGTGTTA TGCCTGCAGA ATTGCCAGTA CCTTACTGCT
  541 TTATCAGGAA TTAATGCGAA AGGGGATACG ATGGCTGATT GAATTAATTA AAGATGATTA
  601 CAATGAAACT GTTCACAAAA AGACAGAAGT TGTGATCACA TTGGATTTCT GTATCAGAAA
  661 CATTGAAAAA ACTGTGAAAG TATATGAAAA GTTGATGAAG ATCAACCTGG AAGCGGCAGA
  721 GTTAGGTGAA ATTTCAGACA TACACACCAA ATTGTTGAGA CTTTCCAGTT CTCAGGGAAC
  781 AATAGAAACC AGTCTTCAGG ATATCGACAG CAGATTATCT CCAGGTGGAT CACTGGCAGA
  841 CGCATGGGCA CATCAAGAAG GCACTCATCC GAAAGACAGA AATGTAGAAA AACTACAAGT
  901 CCTGTTAAAT TGCATGACAG AGATTTACTA TCAGTTCAAA AAAGACAAAG CAGAACGTAG
  961 ATTAGCTTAT AATGAAGAAC AAATCCCACA AATTTGATAA GCAAAAACTG TATTACCATG
 1021 CCACAAAAGC TATGACGCAC TTTACAGATG AATGTGTTAA AAAGTATGAG CATTTTTTGA
 1081 ATAAGTCAGG AAGATGTATA AGAAGGATGC CTCAATCTTA GCAAACAGGT TTATTTATTC
1141 G
//
Primer: pAxCA_R1 RDB14332_A6I5b.seq
>D03656B1_A6I5_1_pAxCA_R1_B06_06_ABI24.ab1
    1 TTTGAACAGG GCATTGGCCA CACCAGCCAC CACCTTCTGA TAGGCAGCCT GCACCTGAGG
   61 AGTGAATTAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC
  121 GCGCAATTAA CCCTCACTAA AGGGAACAAA AGCTGGAGCT CCACCGCGGT GGCGGCCGCC
  181 TAAAGACAGT CAACGTTGCG AAGGCCACCA TCCATGGTTA AAGAGCCAAA CCTTTCTAAA
  241 ATGTGGTTAT TTTCAGCAAG TTCTTTAACC ACCCCTTCCA TCTCTTCCTT TAATTTCTTC
  301 ATACCAAGAG TCATTTCTAC TAATGTGTTA GAACTTGGAT AAATTGGGGT CATGGTATGT
  361 TTGATTCCAC TGGAAGCTGT AAACATTTTC TGAGGCAGAG TTTCTTGTAA CTCATTAGTA
  421 TATTCTTGAT ATTTTGATAC TTCTTCTTCA ATATCAAAAC ACTGATTAGT CAGCGATAAT
  481 AACTGTTTCC TAAGATGAAG CATCTTTCTT ATCCATTCTT CTGACTTATT CAAAAATGCC
  541 TCATACTTTT TAACACATTC ATCTGTAAAG TGCGTCATAG CTTTTGTGGC ATGGTAATAC
  601 AGTTTTTGCT TATCAAATTT GTGGATTTGT TCTTCATTAT AAGCTAATCT ACGTTCTGCT
  661 TTGTCTTTTT TGAACTGATA GTAAATCTCT GTCATGCAAT TTAACAGGAC TTGTAGTTTT
  721 TCTACATTTC TGTCTTTCGG ATGAGTGCCT TCTTGATGTG CCCATGCGTC TGCCAGTGAT
  781 CCACCTGGAG ATAATCTGCT GTCGATATCC TGAAGACTGG TTTCTATTGT TCCCTGAGAA
  841 CTGGAAAGTC TCAACAATTT GGTGTGTATG TCTGAAATTT CACCTAACTC TGCCGCTTCC
  901 AGGTTGATCT TCATCAACTT TTCATATACT TTCACAGTTT TTTTCAATGT TTCTGATACA
  961 GAAATCCAAT GTGATCACAA ACTTCTGTCT TTTTGTGAAC AGTTTCATTG TAAATCATCT
 1021 TTAATTTAAT TCAATCAGCC ATCGTATCCC CTTTCGCAAT TAAATTCCCT TGATAAAGCA
 1081 AGTAAGTACT GGTAACTTCT GCAGGCATTA ACCACACACC CCCATATTAT TGGCCTTAAG
1141 GCCAATGGCC TTAGG
//
Primer: Amp_R RDB14332_A6I5c.seq
>D03656B1_A6I5_1_Amp_R_E06_15_ABI24.ab1
    1 CTTTGTTTCC ATTATTTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGGATACA
   61 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG
  121 TGCCACCTGG GTCGAGCAGT GTGGTTTTCA AGAGGAAGCA AAAAGCCTCT CCACCCAGGC
  181 CTGGAATGTT TCCACCCAAT GTCGAGCAGT GTGGTTTTGC AAGAGGAAGC AAAAAGCCTC
  241 TCCACCCAGG CCTGGAATGT TTCCACCCAA TGTCGAGCAA ACCCCGCCCA GCGTCTTGTC
  301 ATTGGCGAAT TAATTCGAAC ACGCAGATGC AGTCGGGGCG GCGCGGTCCC AGGTCCACTT
  361 CGCATATTAA GGTGACGCGT GTGGCCTCGA ACACCGAGCG ACCCTGCAGC CAATATGGGA
  421 TCGGCCATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
  481 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
  541 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
  601 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
  661 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
  721 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
  781 ATGCGGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAAGCGAAAC
  841 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAAGCCGGTC TTGTCGATCA GGATGATCTG
  901 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG
  961 CCCGACGGGC GATGATCTCG TCGTGACCCA TGGCCGATGC CCTGCTTTGC CCGAATATCA
 1021 TGGTGGAAAT GGGCCCGCTT TTTCTGGAAT TTCAATCGAC TGTGGGCCCG GCTGGTGTGC
 1081 CGGACCGCCT TATTCAGGAC ATAAGCCGTT GGCTAACCGT GAATATTGCT GGAAGAGACC
1141 TTGGTCGGGC GAAAATGAGG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Yamada, T., Constitutive aryl hydrocarbon receptor signaling constrains type I interferon-mediated antiviral innate defense. Nat. Immunol. 17 (6): 687-694 (2016). PMID 27089381.

2018.12.11

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