Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCXN2-FLAG-hTBK1(KD)

Expression vector of FLAG tagged human TBK1, deletion mutant of ubiquitin-like domain (ULD) and coiled coil domain (CC) (306-729 AA).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Yamada, T. et al., Nat. Immunol. 17 (6): 687-694, 2016)
Additional terms and conditions for distribution	Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yamada, T. et al., Nat. Immunol. 17 (6): 687-694, 2016)。
付加的提供条件	CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14331_A6I5p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB14331_A6I5a.seq
>D03656A9_A6I5_1_pAxCA_F1_A05_02_ABI24.ab1     1 GTAAGGGAGG GGCATGGGCA AACATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG    61 CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCAAGC TTAGCCACCA   121 TGGACTACAA AGACGATGAC GATAAAGCAA GGCTCGAGAT GCAGAGCACT TCTAATCATC   181 TGTGGCTTTT ATCTGATATT TTAGGCCAAG GAGCTACTGC AAATGTCTTT CGTGGAAGAC   241 ATAAGAAAAC TGGTGATTTA TTTGCTATCA AAGTATTTAA TAACATAAGC TTCCTTCGTC   301 CAGTGGATGT TCAAATGAGA GAATTTGAAG TGTTGAAAAA ACTCAATCAC AAAAATATTG   361 TCAAATTATT TGCTATTGAA GAGGAGACAA CAACAAGACA TAAAGTACTT ATTATGGAAT   421 TTTGTCCATG TGGGAGTTTA TACACTGTTT TAGAAGAACC TTCTAATGCC TATGGACTAC   481 CAGAATCTGA ATTCTTAATT GTTTTGCGAG ATGTGGTGGG TGGAATGAAT CATCTACGAG   541 AGAATGGTAT AGTGCACCGT GATATCAAGC CAGGAAATAT CATGCGTGTT ATAGGGGAAG   601 ATGGACAGTC TGTGTACAAA CTCACAGATT TTGGTGCAGC TAGAGAATTA GAAGATGATG   661 AGCAGTTTGT TTCTCTGTAT GGCACAGAAG AATATTTGCA CCCTGATATG TATGAGAGAG   721 CAGTGCTAAG AAAAGATCAT CAGAAGAAAT ATGGAGCAAC AGTTGATCTT TGGAGCATTG   781 GGGGTAACAT TTTACCATGC AGCTACTGGA TCACTGCCAT TTAGACCCTT TGAAGGGCCT   841 CGTAGGAATA AAAGAAGTGA TGTATAAAAT AATTACAGGA AAGCCTTCTG GTGCAATATC   901 TGGAGTACAG AAAGCAAGAA AATGGACCAA TTGACTGGAG TGGAGACATG CCTGTTTCTT   961 GCAGTCTTTC TCGGGGGTCT TCAGGTTCTA CTTACCCCCT GTTTCTTGCA AACATCCTTG  1021 AAAGCAGATC AGGAAAAGTG GTTGGGGGTT TTGACAGTTT TTTTTGCAGA AAACTAGTGG  1081 ATATTATAGG GCGGCCGTCA CCCGCGGTGA GCTCAGCTTA GTTCCTTAGT TGAGGGTTAA 1141 AATTGGCGGC GGGT //
Primer: pAxCA_R1 Sequence file: RDB14331_A6I5b.seq
>D03656A9_A6I5_1_pAxCA_R1_B05_05_ABI24.ab1     1 GAACAGAGGC AATTTGGGCC ACACCAGCCA CCACCTTCTG ATAGGCAGCC TGCACCTGAG    61 GAGTGAATTA GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG   121 CGCGCAATTA ACCCTCACTA AAGGGAACAA AAGCTGGAGC TCCACCGCGG TGGCGGCCGC   181 CTATATATCA CTAGTTTCTG CAAAAAACTG GTCAAAACCC CAACACTTTT CCTGATCTGC   241 TTCAAGGATG TTTGCAAGAA CAGGGGTAAG TAGAACCTGA AGACCCCGAG AAAGACTGCA   301 AGAAACAGGC ATGTCTCCAC TCCAGTCAAT TGGTCCATTT TCTGCTTTCT GTACTCCAGA   361 TATTGCACCA GAAGGCTTTC CTGTAATTAT TTTATACATC ACTTCTTTAT TCCTACGAGG   421 CCCTTCAAAG GGTCTAAATG GCAGTGATCC AGTAGCTGCA TGGTAAAATG TTACCCCAAT   481 GCTCCAAAGA TCAACTGTTG CTCCATATTT CTTCTGATGA TCTTTTCTTA GCACTGCTCT   541 CTCATACATA TCAGGGTGCA AATATTCTTC TGTGCCATAC AGAGAAACAA ACTGCTCATC   601 ATCTTCTAAT TCTCTAGCTG CACCAAAATC TGTGAGTTTG TACACAGACT GTCCATCTTC   661 CCCTATAACA CGCATGATAT TTCCTGGCTT GATATCACGG TGCACTATAC CATTCTCTCG   721 TAGATGATTC ATTCCACCCA CCACATCTCG CAAAACAATT AAGAATTCAG ATTCTGGTAG   781 TCCATAGGCA TTAGAAGGTT CTTCTAAAAC AGTGTATAAA CTCCCACATG GACAAAATTC   841 CATAATAAGT ACTTTATGTC TTGTTGTTGT CTCCTCTTCA ATAGCAAATA ATTTGACAAT   901 ATTTTTGTGA TTGAGTTTTT TTCAACACTT CAAATTCTCT CATTTGAAAC ATCCACTGGA   961 CGAAGGAAGC TTATGTTATT AAATACTTTT GATAGCAAAT AAATCACCAG TTTTCTTTAT  1021 GTCTTTCCAC GAAAGACATT TGGCAGTAGC TCCTTGGGCC TAAAAATATC AGATAAAAGC  1081 ACAGATGATT TAGAAGTGCT CTGCATCTCG AAGCTGGCAT TAATCGTCAT CGATCCTTTG 1141 TTAAGTTCCA GATG //
Primer: Amp_R Sequence file: RDB14331_A6I5c.seq
>D03656A9_A6I5_1_Amp_R_G07_07_ABI08.ab1     1 CTTTTTTTTC ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT    61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC   121 CACCTGGGTC GAGCAGTGTG GTTTTCAAGA GGAAGCAAAA AGCCTCTCCA CCCAGGCCTG   181 GAATGTTTCC ACCCAATGTC GAGCAGTGTG GTTTTGCAAG AGGAAGCAAA AAGCCTCTCC   241 ACCCAGGCCT GGAATGTTTC CACCCAATGT CGAGCAAACC CCGCCCAGCG TCTTGTCATT   301 GGCGAATTAA TTCGAACACG CAGATGCAGT CGGGGCGGCG CGGTCCCAGG TCCACTTCGC   361 ATATTAAGGT GACGCGTGTG GCCTCGAACA CCGAGCGACC CTGCAGCCAA TATGGGATCG   421 GCCATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC   481 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA   541 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG   601 CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG   661 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG   721 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG   781 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC   841 ATCGAGCGAG CACGTACTCG GATGGAAGCC CGGTCTTGTC GATCAGGATG ATCTGGACGA   901 AGAGCATCAA GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA   961 CGCGATGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAATG  1021 GCCGCTTTTT CTGGATTCAT CGACTGGTGG CCGCCTGGGT GTGGCGACCG GCTATCAGGA  1081 CATAGCGTGG CTACCGTGAT ATGCTGAAGA GCCTGGCGGC GATGGCTGAC GCTCGTCGTG  1141 CTAACGTATA GCGGCTCCGA TCCAGGCCAT CGCTCAATCG GCCTCTTGAC GAGAGTCCTT 1201 CG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl