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pCXN2-FLAG-hTBK1(KD)

Expression vector of FLAG tagged human TBK1, deletion mutant of ubiquitin-like domain (ULD) and coiled coil domain (CC) (306-729 AA).

Catalog number RDB14331
Resource name pCXN2-FLAG-hTBK1(KD)
Clone info. Expression vector driven by the CAG promoter for FLAG tagged human TANK binding kinase 1 (TBK1) mutant; deletion of ubiquitin-like domain (ULD) and coiled coil domain (CC) (306-729 AA). See Fig. S4d of Yamada, T., Nat. Immunol, 17 (6): 687-694, 2016 for details.
Expression was confirmed by the deposotor with immunoprecipitation and immunoblotting.
Vector backbone pCXN2-Flag (Plasmid)
Selectable markers Amp^r
Gene/insert name human TBK1 cDNA
Depositor|Developer Takaoka, Akinori |
 
Other clones in our bank human TBK1 (NCBI Gene 29110) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Yamada, T. et al., Nat. Immunol. 17 (6): 687-694, 2016)
Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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RDB14331 pCXN2-FLAG-hTBK1(KD) DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Yamada, T., Constitutive aryl hydrocarbon receptor signaling constrains type I interferon-mediated antiviral innate defense. Nat. Immunol. 17 (6): 687-694 (2016). PMID 27089381.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14331_A6I5p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB14331_A6I5a.seq check
>D03656A9_A6I5_1_pAxCA_F1_A05_02_ABI24.ab1
    1 GTAAGGGAGG GGCATGGGCA AACATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG
   61 CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCAAGC TTAGCCACCA
  121 TGGACTACAA AGACGATGAC GATAAAGCAA GGCTCGAGAT GCAGAGCACT TCTAATCATC
  181 TGTGGCTTTT ATCTGATATT TTAGGCCAAG GAGCTACTGC AAATGTCTTT CGTGGAAGAC
  241 ATAAGAAAAC TGGTGATTTA TTTGCTATCA AAGTATTTAA TAACATAAGC TTCCTTCGTC
  301 CAGTGGATGT TCAAATGAGA GAATTTGAAG TGTTGAAAAA ACTCAATCAC AAAAATATTG
  361 TCAAATTATT TGCTATTGAA GAGGAGACAA CAACAAGACA TAAAGTACTT ATTATGGAAT
  421 TTTGTCCATG TGGGAGTTTA TACACTGTTT TAGAAGAACC TTCTAATGCC TATGGACTAC
  481 CAGAATCTGA ATTCTTAATT GTTTTGCGAG ATGTGGTGGG TGGAATGAAT CATCTACGAG
  541 AGAATGGTAT AGTGCACCGT GATATCAAGC CAGGAAATAT CATGCGTGTT ATAGGGGAAG
  601 ATGGACAGTC TGTGTACAAA CTCACAGATT TTGGTGCAGC TAGAGAATTA GAAGATGATG
  661 AGCAGTTTGT TTCTCTGTAT GGCACAGAAG AATATTTGCA CCCTGATATG TATGAGAGAG
  721 CAGTGCTAAG AAAAGATCAT CAGAAGAAAT ATGGAGCAAC AGTTGATCTT TGGAGCATTG
  781 GGGGTAACAT TTTACCATGC AGCTACTGGA TCACTGCCAT TTAGACCCTT TGAAGGGCCT
  841 CGTAGGAATA AAAGAAGTGA TGTATAAAAT AATTACAGGA AAGCCTTCTG GTGCAATATC
  901 TGGAGTACAG AAAGCAAGAA AATGGACCAA TTGACTGGAG TGGAGACATG CCTGTTTCTT
  961 GCAGTCTTTC TCGGGGGTCT TCAGGTTCTA CTTACCCCCT GTTTCTTGCA AACATCCTTG
 1021 AAAGCAGATC AGGAAAAGTG GTTGGGGGTT TTGACAGTTT TTTTTGCAGA AAACTAGTGG
 1081 ATATTATAGG GCGGCCGTCA CCCGCGGTGA GCTCAGCTTA GTTCCTTAGT TGAGGGTTAA
1141 AATTGGCGGC GGGT
//
Primer: pAxCA_R1
Sequence file: RDB14331_A6I5b.seq check
>D03656A9_A6I5_1_pAxCA_R1_B05_05_ABI24.ab1
    1 GAACAGAGGC AATTTGGGCC ACACCAGCCA CCACCTTCTG ATAGGCAGCC TGCACCTGAG
   61 GAGTGAATTA GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG
  121 CGCGCAATTA ACCCTCACTA AAGGGAACAA AAGCTGGAGC TCCACCGCGG TGGCGGCCGC
  181 CTATATATCA CTAGTTTCTG CAAAAAACTG GTCAAAACCC CAACACTTTT CCTGATCTGC
  241 TTCAAGGATG TTTGCAAGAA CAGGGGTAAG TAGAACCTGA AGACCCCGAG AAAGACTGCA
  301 AGAAACAGGC ATGTCTCCAC TCCAGTCAAT TGGTCCATTT TCTGCTTTCT GTACTCCAGA
  361 TATTGCACCA GAAGGCTTTC CTGTAATTAT TTTATACATC ACTTCTTTAT TCCTACGAGG
  421 CCCTTCAAAG GGTCTAAATG GCAGTGATCC AGTAGCTGCA TGGTAAAATG TTACCCCAAT
  481 GCTCCAAAGA TCAACTGTTG CTCCATATTT CTTCTGATGA TCTTTTCTTA GCACTGCTCT
  541 CTCATACATA TCAGGGTGCA AATATTCTTC TGTGCCATAC AGAGAAACAA ACTGCTCATC
  601 ATCTTCTAAT TCTCTAGCTG CACCAAAATC TGTGAGTTTG TACACAGACT GTCCATCTTC
  661 CCCTATAACA CGCATGATAT TTCCTGGCTT GATATCACGG TGCACTATAC CATTCTCTCG
  721 TAGATGATTC ATTCCACCCA CCACATCTCG CAAAACAATT AAGAATTCAG ATTCTGGTAG
  781 TCCATAGGCA TTAGAAGGTT CTTCTAAAAC AGTGTATAAA CTCCCACATG GACAAAATTC
  841 CATAATAAGT ACTTTATGTC TTGTTGTTGT CTCCTCTTCA ATAGCAAATA ATTTGACAAT
  901 ATTTTTGTGA TTGAGTTTTT TTCAACACTT CAAATTCTCT CATTTGAAAC ATCCACTGGA
  961 CGAAGGAAGC TTATGTTATT AAATACTTTT GATAGCAAAT AAATCACCAG TTTTCTTTAT
 1021 GTCTTTCCAC GAAAGACATT TGGCAGTAGC TCCTTGGGCC TAAAAATATC AGATAAAAGC
 1081 ACAGATGATT TAGAAGTGCT CTGCATCTCG AAGCTGGCAT TAATCGTCAT CGATCCTTTG
1141 TTAAGTTCCA GATG
//
Primer: Amp_R
Sequence file: RDB14331_A6I5c.seq check
>D03656A9_A6I5_1_Amp_R_G07_07_ABI08.ab1
    1 CTTTTTTTTC ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
   61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
  121 CACCTGGGTC GAGCAGTGTG GTTTTCAAGA GGAAGCAAAA AGCCTCTCCA CCCAGGCCTG
  181 GAATGTTTCC ACCCAATGTC GAGCAGTGTG GTTTTGCAAG AGGAAGCAAA AAGCCTCTCC
  241 ACCCAGGCCT GGAATGTTTC CACCCAATGT CGAGCAAACC CCGCCCAGCG TCTTGTCATT
  301 GGCGAATTAA TTCGAACACG CAGATGCAGT CGGGGCGGCG CGGTCCCAGG TCCACTTCGC
  361 ATATTAAGGT GACGCGTGTG GCCTCGAACA CCGAGCGACC CTGCAGCCAA TATGGGATCG
  421 GCCATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC
  481 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA
  541 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG
  601 CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG
  661 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG
  721 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG
  781 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC
  841 ATCGAGCGAG CACGTACTCG GATGGAAGCC CGGTCTTGTC GATCAGGATG ATCTGGACGA
  901 AGAGCATCAA GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA
  961 CGCGATGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAATG
 1021 GCCGCTTTTT CTGGATTCAT CGACTGGTGG CCGCCTGGGT GTGGCGACCG GCTATCAGGA
 1081 CATAGCGTGG CTACCGTGAT ATGCTGAAGA GCCTGGCGGC GATGGCTGAC GCTCGTCGTG
 1141 CTAACGTATA GCGGCTCCGA TCCAGGCCAT CGCTCAATCG GCCTCTTGAC GAGAGTCCTT
1201 CG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yamada, T., Constitutive aryl hydrocarbon receptor signaling constrains type I interferon-mediated antiviral innate defense. Nat. Immunol. 17 (6): 687-694 (2016). PMID 27089381.

2021.03.26

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