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pCXN2-FLAG-hTBK1(WT)

Expression vector of FLAG tagged human TBK1, wild type.

Catalog number RDB14330
Resource name pCXN2-FLAG-hTBK1(WT)
Clone info. Expression vector driven by the CAG promoter for FLAG tagged human TANK binding kinase 1 (TBK1); wild type. See Fig. S4d of Yamada, T., Nat. Immunol, 17 (6): 687-694, 2016 for details.
Expression was confirmed by the deposotor with immunoprecipitation and immunoblotting.
Vector backbone pCXN2-Flag (Plasmid)
Selectable markers Amp^r
Gene/insert name human TBK1 cDNA
Depositor Takaoka, Akinori |
 
Other clones in our bank human TBK1 (NCBI Gene 29110) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Yamada, T. et al., Nat. Immunol. 17 (6): 687-694, 2016)
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RDB14330 pCXN2-FLAG-hTBK1(WT) DNA solution

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References and tips

Electronic file

Featured content

References

original Yamada, T., Constitutive aryl hydrocarbon receptor signaling constrains type I interferon-mediated antiviral innate defense. Nat. Immunol. 17 (6): 687-694 (2016). PMID 27089381.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14330_A6I5p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB14330_A6I5a.seq
>D03656A8_A6I5_1_pAxCA_F1_A04_01_ABI24.ab1
    1 GAGGGGGAGG TTTGTCTACA TGTTATGCCT TCTTCTTTTT CCTACAGCTC CTGGGCAACG
   61 TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT CAAGCTTAGC CACCATGGAC
  121 TACAAAGACG ATGACGATAA AGCAAGGCTC GAGATGCAGA GCACTTCTAA TCATCTGTGG
  181 CTTTTATCTG ATATTTTAGG CCAAGGAGCT ACTGCAAATG TCTTTCGTGG AAGACATAAG
  241 AAAACTGGTG ATTTATTTGC TATCAAAGTA TTTAATAACA TAAGCTTCCT TCGTCCAGTG
  301 GATGTTCAAA TGAGAGAATT TGAAGTGTTG AAAAAACTCA ATCACAAAAA TATTGTCAAA
  361 TTATTTGCTA TTGAAGAGGA GACAACAACA AGACATAAAG TACTTATTAT GGAATTTTGT
  421 CCATGTGGGA GTTTATACAC TGTTTTAGAA GAACCTTCTA ATGCCTATGG ACTACCAGAA
  481 TCTGAATTCT TAATTGTTTT GCGAGATGTG GTGGGTGGAA TGAATCATCT ACGAGAGAAT
  541 GGTATAGTGC ACCGTGATAT CAAGCCAGGA AATATCATGC GTGTTATAGG GGAAGATGGA
  601 CAGTCTGTGT ACAAACTCAC AGATTTTGGT GCAGCTAGAG AATTAGAAGA TGATGAGCAG
  661 TTTGTTTCTC TGTATGGCAC AGAAGAATAT TTGCACCCTG ATATGTATGA GAGAGCAGTG
  721 CTAAGAAAAG ATCATCAGAA GAAATATGGA GCAACAGTTG ATCTTTGGAG CATTGGGGTA
  781 ACATTTTACC ATGCAGCTAC TGGATCACTG CCATTTAGAC CCTTTGAAGG GCCTCGTAGG
  841 AATAAAGAAG TGATGTATAA AATAATTACA GGAAAGCCTT CTGGTGCAAT ATCTGGAGTA
  901 CAGAAAGCAG AAAAATGGAC CAATTGACTG GAGTGGAGAC ATGCCTGTTT CTTGCAGTCT
  961 TTCTCGGGGT CTTCAGTTCT ACTTACCCCT GTTTCTTGCA ACATCCCTTG AAGCAGATCA
 1021 GGAAAAGTGT TGGGGGTTTT GACCAGTTTT TTGCAGAAAC TAGTTGATAT ACCTCCACGG
 1081 AATGTAATTC ATGTTTTCGC TACAACCAAT GGACAGGCTC AATAAGATTT TTATAATTAC
1141 C
//
Primer: pAxCA_R1 RDB14330_A6I5b.seq
>D03656A8_A6I5_1_pAxCA_R1_B04_04_ABI24.ab1
    1 TTGACAAGGG CAATTGGCCA CACCAGCCAC CACCTTCTGA TAGGCAGCCT GCACCTGAGG
   61 AGTGAATTAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC
  121 GCGCAATTAA CCCTCACTAA AGGGAACAAA AGCTGGAGCT CCACCGCGGT GGCGGCCGCC
  181 TAAAGACAGT CAACGTTGCG AAGGCCACCA TCCATGGTTA AAGAGCCAAA CCTTTCTAAA
  241 ATGTGGTTAT TTTCAGCAAG TTCTTTAACC ACCCCTTCCA TCTCTTCCTT TAATTTCTTC
  301 ATACCAAGAG TCATTTCTAC TAATGTGTTA GAACTTGGAT AAATTGGGGT CATGGTATGT
  361 TTGATTCCAC TGGAAGCTGT AAACATTTTC TGAGGCAGAG TTTCTTGTAA CTCATTAGTA
  421 TATTCTTGAT ATTTTGATAC TTCTTCTTCA ATATCAAAAC ACTGATTAGT CAGCGATAAT
  481 AACTGTTTCC TAAGATGAAG CATCTTTCTT ATCCATTCTT CTGACTTATT CAAAAATGCC
  541 TCATACTTTT TAACACATTC ATCTGTAAAG TGCGTCATAG CTTTTGTGGC ATGGTAATAC
  601 AGTTTTTGCT TATCAAATTT GTGGATTTGT TCTTCATTAT AAGCTAATCT ACGTTCTGCT
  661 TTGTCTTTTT TGAACTGATA GTAAATCTCT GTCATGCAAT TTAACAGGAC TTGTAGTTTT
  721 TCTACATTTC TGTCTTTCGG ATGAGTGCCT TCTTGATGTG CCCATGCGTC TGCCAGTGAT
  781 CCACCTGGAG ATAATCTGCT GTCGATATCC TGAAGACTGG TTTCTATTGT TCCCTGAGAA
  841 CTGGAAAGTC TCAACAATTT GGTGTGTATG TCTGAAATTT CACCTAACTC TGCCGCTTCC
  901 AGGTTGATCT TCATCAACTT TTTCATATAC TTTCACAGTT TTTTTCAATG TTTCTGATAC
  961 AGAAATCCAA ATGTGATCAC AACTTTCTGT CTTTTTGTGA ACAGTTTCAT TGTAATCATC
 1021 TTTAATTTAA TTTCAATCAG CCATCGTATC CCCTTTTCGC ATTATTTCCT TGGATAAGCC
 1081 AGTAAGTACT GGGCATTTCT GCAGGCATAA CAACACAACC CTGATATGAC CATTAGCCAG
1141 TGGCCTAG
//
Primer: Amp_R RDB14330_A6I5c.seq
>D03656A8_A6I5_1_Amp_R_F07_06_ABI08.ab1
    1 TTCCCTTTTT CAAATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
   61 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG
  121 CCACCTGGGT CGAGCAGTGT GGTTTTCAAG AGGAAGCAAA AAGCCTCTCC ACCCAGGCCT
  181 GGAATGTTTC CACCCAATGT CGAGCAGTGT GGTTTTGCAA GAGGAAGCAA AAAGCCTCTC
  241 CACCCAGGCC TGGAATGTTT CCACCCAATG TCGAGCAAAC CCCGCCCAGC GTCTTGTCAT
  301 TGGCGAATTA ATTCGAACAC GCAGATGCAG TCGGGGCGGC GCGGTCCCAG GTCCACTTCG
  361 CATATTAAGG TGACGCGTGT GGCCTCGAAC ACCGAGCGAC CCTGCAGCCA ATATGGGATC
  421 GGCCATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT
  481 CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC
  541 AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT
  601 GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT
  661 GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA
  721 GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT
  781 GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG
  841 CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA
  901 AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA
  961 CGGCGATGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA
 1021 TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGAC
 1081 ATAGCCGTGC TACCCGTGAT TATTGCTGGA GAGCTGCGCG ATGGCTGACG GCTTCCTCGT
 1141 GCCTTACGGT ATCGCCGCCT CGATTCCACG CATCGCTTCT TATTCGCCCT TCTTTGGACC
1201 GAAGAG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Yamada, T., Constitutive aryl hydrocarbon receptor signaling constrains type I interferon-mediated antiviral innate defense. Nat. Immunol. 17 (6): 687-694 (2016). PMID 27089381.

2018.12.11

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