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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pFastBac1-GST-hTIPARP(H532A) (#RDB14328)

Baculovirus expression vector of GST tagged human TIPARP, H532A mutant.

Clone info. Baculovirus expression vector of GST tagged human TCDD inducible poly (ADP-ribose) polymerase (TIPARP) mutant; H532A.
Comment Expression was confirmed by the deposotor with autoradiographic analysis and immunoblot.
Vector backbone pFastBac1 (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name human TIPARP cDNA
Depositor|Developer Takaoka, Akinori |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Yamada, T. et al., Nat. Immunol. 17 (6): 687-694, 2016)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yamada, T. et al., Nat. Immunol. 17 (6): 687-694, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14328 pFastBac1-GST-hTIPARP(H532A) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pFastBac1-GST-hTIPARP(H532A) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14328).

Reference section:

Yamada, T., Horimoto, H., Kameyama, T., Hayakawa, S., Yamato, H., Dazai, M., Takada, A., Kida, H., Bott, D., Zhou, A.C., Hutin, D., Watts, T.H., Asaka, M., Matthews, J., Takaoka, A., Constitutive aryl hydrocarbon receptor signaling constrains type I interferon-mediated antiviral innate defense. Nat. Immunol. 17 (6): 687-694 (2016). PMID 27089381. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14328_A6I5p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pPolyhedrin_F
Sequence file: RDB14328_A6I5a.seq check
>D03656A6_A6I5_1_pPolyhedrin_F_E08_05_ABI08.ab1
    1 AAAATTTTAC TGTTTTCGTA ACAGTTTTGT AATAAAAAAA CCTATAAATA TTCCGGATTA
   61 TTCATACCGT CCCACCATCG GGCGCGGATC TCCACCATGT CCCCTATACT AGGTTATTGG
  121 AAAATTAAGG GCCTTGTGCA ACCCACTCGA CTTCTTTTGG AATATCTTGA AGAAAAATAT
  181 GAAGAGCATT TGTATGAGCG CGATGAAGGT GATAAATGGC GAAACAAAAA GTTTGAATTG
  241 GGTTTGGAGT TTCCCAATCT TCCTTATTAT ATTGATGGTG ATGTTAAATT AACACAGTCT
  301 ATGGCCATCA TACGTTATAT AGCTGACAAG CACAACATGT TGGGTGGTTG TCCAAAAGAG
  361 CGTGCAGAGA TTTCAATGCT TGAAGGAGCG GTTTTGGATA TTAGATACGG TGTTTCGAGA
  421 ATTGCATATA GTAAAGACTT TGAAACTCTC AAAGTTGATT TTCTTAGCAA GCTACCTGAA
  481 ATGCTGAAAA TGTTCGAAGA TCGTTTATGT CATAAAACAT ATTTAAATGG TGATCATGTA
  541 ACCCATCCTG ACTTCATGTT GTATGACGCT CTTGATGTTG TTTTATACAT GGACCCAATG
  601 TGCCTGGATG CGTTCCCAAA ATTAGTTTGT TTTAAAAAAC GTATTGAAGC TATCCCACAA
  661 ATTGATAAGT ACTTGAAATC CAGCAAGTAT ATAGCATGGC CTTTGCAGGG CTGGCAAGCC
  721 ACGTTTGGTG GTGGCGACCA TCCTCCAAAA TCGGATCTGG AAGTTCTGTT CCAGGGGCCC
  781 CTGGGATCCC CGGAATTCGT GATTCTCGAC ATGGAAATGG AAACCACCGA ACCTGAGCCA
  841 GACTGTGTAG TGCAGCCTCC CTCTCCTCCT GATGACTTTT CATGCCAAAT GAGACTCTCT
  901 GAGAAGATCA CTCCATTGAA GACTTGTTTT AAGAAAAAGG ATCAGAAAAG ATTGGGAACT
  961 GGAACCCTGA GGTCTTTGAG GCCAATATTA AACACTCTTC TAGAATCTGG CTCACTTGAT
 1021 GGGGTTTTTA GATCTAGGAC CAGAGTACAG ATGAGACAGC TTACATGAAC TATGATGAGA
 1081 AAGCCATGGA AATCATTCAT CATGCCCACC AGCAGAAAAT AATATGTTCT GTTCTGATCT
1141 TGATAGACAA ATGTGGGGAC CAATACTGAG TCATCTCCTG AAGCTCAACG AAGTGGATC
//
Primer: pGEX-F
Sequence file: RDB14328_A6I5b.seq check
>D03656A6_A6I5_1_pGEX-F_D07_04_ABI08.ab1
    1 GGCATCGTCA AAATCGGATC TGGAGTTCTG TTCCAGGGGC CCCTGGGATC CCCGGAATTC
   61 GTGATTCTCG ACATGGAAAT GGAAACCACC GAACCTGAGC CAGACTGTGT AGTGCAGCCT
  121 CCCTCTCCTC CTGATGACTT TTCATGCCAA ATGAGACTCT CTGAGAAGAT CACTCCATTG
  181 AAGACTTGTT TTAAGAAAAA GGATCAGAAA AGATTGGGAA CTGGAACCCT GAGGTCTTTG
  241 AGGCCAATAT TAAACACTCT TCTAGAATCT GGCTCACTTG ATGGGGTTTT TAGATCTAGG
  301 AACCAGAGTA CAGATGAGAA CAGCTTACAT GAACCTATGA TGAAGAAAGC CATGGAAATC
  361 AATTCATCAT GCCCACCAGC AGAAAATAAT ATGTCTGTTC TGATTCCTGA TAGGACAAAT
  421 GTTGGGGACC AGATACCGGA AGCCCATCCT TCCACTGAAG CTCCAGAACG AGTGGTTCCA
  481 ATCCAAGATC ACAGCTTTCC ATCAGAAACC CTCAGTGGGA CGGTGGCAGA TTCCACACCA
  541 GCTCACTTCC AGACTGATCT TTTGCACCCA GTTTCAAGTG ATGTTCCTAC TAGTCCTGAC
  601 TGCTTAGACA AAGTCATAGA TTATGTTCCA GGCATTTTCC AAGAAAACAG TTTTACAATC
  661 CAATACATTC TGGACACCAG TGATAAGCTG AGTACTGAGC TCTTTCAGGA CAAAAGTGAA
  721 GAGGCTTCCC TTGACCTCGT GTTTGAGCTG GTGAACCAGT TGCAGTACCA CACTCACCAA
  781 GAGAACGGAA TTGAAATTTG CATGGACTTT CTGCAAGGCA CTTGTATTTA TGGCAGGGAT
  841 TGTTTGAAGC ACCACACTGT CTTGCCATAT CATTGGCAGA TCAAAAGGAC AACTACTCAA
  901 AAGTGGCAGA GTGTATTCAA TGATTCTCAG GAGCACTTGG AAAGATTTTA CTGTAACCCA
  961 GAAAATGATA GAATGAGAAT GAAGTATGGA GGACAAGAAT TTTGGGCAGA TTTGAATGCC
 1021 ATGAACGTGT ATGAAACAAC TGAAATTTGA CCACTACGAA GGCTGTCCAC ACCACCCTCT
 1081 AGCATGTCAA CTCTATTTAA CACACAGTCT GAATCTCGTA GGAACACTTT GGAATGAGAG
1141 AGTATCCCGA GTCGTCATTC GATTGATTGG AGAGCTAACT CTCTCCGAT
//
Primer: SV40polyA-R
Sequence file: RDB14328_A6I5c.seq check
>D03656A6_A6I5_1_SV40polyA-R_G06_21_ABI24.ab1
    1 AGATTTTGGA AGTTTTTTAA GCAAGTAAAA CCTCTACAAT GTGGTATGGC TGATTATGAT
   61 CCTCTAGTAC TTCTCGACAA GCTTGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC
  121 GAGGCAGATC GTCAGTCAGT CACGATGCGG CCGCTCAAAT GGAAACAGTG TTACTGACTT
  181 CTTCATATTG GATAACAAAA TAAGGGTAAC TCTGGTCATC ATTAAAAATG ACAAAAATCT
  241 GAGGCTCAAA GAAATTATCC ACACAAGAGT CATAAAGGTC ACTGGTGACA CTGCCAGGAT
  301 TGACTGGCGG GGGCCTTCTC ATGCCATGAC TGCCCATTGT GTATCTGCCC GTCAGCACTT
  361 TGGCCAGAAA CATGAAGTGG ACTCCTTTGG AGGACTTCTT AGAAAAGTTA TGAGAGTAGC
  421 TTGCCTTCTT TGCAAAATAA CTGCCTTGTC CAAACATTGT AGCATGCTTT CCACAGACTC
  481 GAGGGTCAAA GTTGTGTTTG CAGATTCCAT CTACCACATC CTGGGATGTT CCAGCAAATA
  541 AATGTCTCTC ATTTATTATC CTGTCACGGC CAAACATTTT CCTGTTCATA TATTCCTTTT
  601 TCCTTTTATA TTTCTCCCAA AGAAACTGGT TTTGGACTCT CAATATCTGC AAAATTCTGT
  661 ATTTAAACTC AGGCACAGTC TTATGAAAAA GATTGTAAAT GATCCGATAA CTTTTATCCT
  721 CTGCAGAAAC AGGGACTTGG ATGAAGTCCT GAGATGGATG CATATAAACC CAAGTTTCAG
  781 GGTAAAAGTT TGCTGAAGTG ACCCCATCTG GGCAGATAAT TTGTGATGAT GAAGTTGCTT
  841 CAAGAGGTGG AGGAGCTTGT GTGGGAACCC CACCAAGTGT CTGTAAATAT GGGAAGCAGT
  901 ATAAAACAGG AGCGGAAGAG GGGTCTCCTT TTTTATCTCT CTCCTGAAGA ATGAATTGTG
  961 GAGGATGTAG TGGTTTATTT CCACATCATA AAATCGAAAC CCTCTTTTCA GACCCCGAGA
 1021 AGTTTGGGCT TTCTTCATCC ATCGGAAATG AACAGACTCG GGAATACTTC TTCTCCATCC
 1081 AAGTTGGTTC CCTTAACGAG ATTCCAGGAC TGTGTGTTGG TAATAGAGTT TGGAACAATT
1141 TGGCCTTAG
//
Primer: f1ori_R3
Sequence file: RDB14328_A6I5d.seq check
>D03656A6_A6I5_1_f1ori_R3_D11_11_ABI24.ab1
    1 GACCTTGGCA TTCAGCTGCA ATAAGCGTTG ATATTCAGTC AATTACAAAC ATTAATAACG
   61 AAGAGATGAC AGAAAAATTT TCATTCTGTG ACAGAGAAAA AGTAGCCGAA GATGACGGTT
  121 TGTCACATGG AGTTGGCAGG ATGTTTGATT AAAAACATAA CAGGAAGAAA AATGCCCCGC
  181 TGTGGGCGGA CAAAATAGTT GGGAACTGGG AGGGGTGGAA ATGGAGTTTT TAAGGATTAT
  241 TTAGGGAAGA GTGACAAAAT AGATGGGAAC TGGGTGTAGC GTCGTAAGCT AATACGAAAA
  301 TTAAAAATGA CAAAATAGTT TGGAACTAGA TTTCACTTAT CTGGTTCGGA TCTCCTAGGC
  361 TCAAGCAGTG ATCAGATCCA GACATGATAA GATACATTGA TGAGTTTGGA CAAACCACAA
  421 CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT GCTTTATTTG
  481 TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC
  541 AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA
  601 TGGCTGATTA TGATCCTCTA GTACTTCTCG ACAAGCTTGT GTCAGAGGTT TTCACCGTCA
  661 TCACCGAAAC GCGCGAGGCA GATCGTCAGT CAGTCACGAT GCGGCCGCTC AAATGGAAAC
  721 AGTGTTACTG ACTTCTTCAT ATTGGATAAC AAAATAAGGG TAACTCTGGT CATCATTAAA
  781 AATGACAAAA ATCTGAGGCT CAAAGAAATT ATCCACACAA GAGTCATAAA GGTCACTGGT
  841 GACACTGCCA GGATTGACTG GCGGGGGCCT TCTCAATGCC ATGACTGCCC ATTGTGTATC
  901 TGCCCGTCAG CACTTTGGGC CAGAAACATG AAGTGGGACT CCTTTTGGAG GACTTCTTTA
  961 GAAAAGTTAT GAGAGTAGCT TTGCCCTTTC TTTGCAAAAT TACCTGCCCT TGTCCAACCA
 1021 TTGTAAGCAT GCTTTCCAAC AGACTTCGAG GTCAAGCTGT GTTGGCAGAT TCAATCTACC
1081 CACAATCTTG GATGGTCAGC CAATTAATGA TCCTCTCCAA ATTAAATTAT ATCCCT
//
Primer: ColE1ori_F
Sequence file: RDB14328_A6I5e.seq check
>D03656A6_A6I5_1_ColE1ori_F_E11_14_ABI24.ab1
    1 GGACAGACTG ATCGTACGCA CATCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG
   61 GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC
  121 TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT
  181 ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA
  241 GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT
  301 ATTTCACACC GCATAGACCA GCCGCGTAAC CTGGCAAAAT CGGTTACGGT TGAGTAATAA
  361 ATGGATGCCC TGCGTAAGCG GGTGTGGGCG GACAATAAAG TCTTAAACTG AACAAAATAG
  421 ATCTAAACTA TGACAATAAA GTCTTAAACT AGACAGAATA GTTGTAAACT GAAATCAGTC
  481 CAGTTATGCT GTGAAAAAGC ATACTGGACT TTTGTTATGG CTAAAGCAAA CTCTTCATTT
  541 TCTGAAGTGC AAATTGCCCG TCGTATTAAA GAGGGGCGTG GCCAAGGGCA TGGTAAAGAC
  601 TATATTCGCG GCGTTGTGAC AATTTACCGA ACAACTCCGC GGCCGGGAAG CCGATCTCGG
  661 CTTGAACGAA TTGTTAGGTG GCGGTACTTG GGTCGATATC AAAGTGCATC ACTTCTTCCC
  721 GTATGCCCAA CTTTGTATAG AGAGCCACTG CGGGATCGTC ACCGTAATCT GCTTGCACGT
  781 AGATCACATA AGCACCAAGC GCGTTGGCCT CATGCTTGAG GAGATTGATG AGCGCGGTGG
  841 CAATGCCCCT GCCTCCGGTG CTCGCCCGGA GACTGCGAGA TCATAAGATA TAGATCTCAA
  901 CTACGCGGCT GCTCAAAACT TGGGGCAGGA ACGTTAAGCC CGCGAAGAAC GCCCAACAAA
  961 CCGCTTTCTT GGGTCGAAAG GGCAGGCAAG GCGGCGAATG AATGTCTTTA CCTACGGGAG
 1021 CAAAGGTTTC CCCGGAGGGT AATCGGGAGT TCTGGGCTTG AATTGTTTGG GGAAGTAACG
 1081 TTGGACTTAA CGCTCTTCCG GAACTCACGG AAATCGGAAA AGGAATTCCA TAGCAAGGTA
1141 CAATCGCTCT CCGGCTC
//
Primer: Pcpro_F
Sequence file: RDB14328_A6I5f.seq check
>D03656A6_A6I5_1_Pcpro_F_B01_02_ABI08.ab1
    1 CAGCTGGAAA GCGGCCTCCG CAACTGGTCC AGAACCTTGA CCGAACGCAG CGGTGGTAAC
   61 GGCGCAGTGG CGGTTTTCAT GGCTTGTTAG GACTGTTTTT TTGTACAGTC TATGCCTCGG
  121 GCATCCAAGC AGCAAGCGCG TTACGCCGTG GGTCGATGTT TGATGTTATG GAGCAGCAAC
  181 GATGTTACGC AGCAGCAACG ATGTTACGCA GCAGGGCAGT CGCCCTAAAA CAAAGTTAGG
  241 TGGCTCAAGT ATGGGCATCA TTCGCACATG TAGGCTCGGC CCTGACCAAG TCAAATCCAT
  301 GCGGGCTGCT CTTGATCTTT TCGGTCGTGA GTTCGGAGAC GTAGCCACCT ACTCCCAACA
  361 TCAGCCGGAC TCCGATTACC TCGGGAACTT GCTCCGTAGT AAGACATTCA TCGCGCTTGC
  421 TGCCTTCGAC CAAGAAGCGG TTGTTGGCGC TCTCGCGGCT TACGTTCTGC CCAAGTTTGA
  481 GCAGCCGCGT AGTGAGATCT ATATCTATGA TCTCGCAGTC TCCGGCGAGC ACCGGAGGCA
  541 GGGCATTGCC ACCGCGCTCA TCAATCTCCT CAAGCATGAG GCCAACGCGC TTGGTGCTTA
  601 TGTGATCTAC GTGCAAGCAG ATTACGGTGA CGATCCCGCA GTGGCTCTCT ATACAAAGTT
  661 GGGCATACGG GAAAGAAGTG ATGCACTTTG ATATCGACCC AAGTACCGCC ACCTAAACAA
  721 TTCGTTCAAG CCGAGATCGG CTTCCCGGCC GCGGAGTTGT TCGGTAAATT GTCACAACGC
  781 CGCGAATATA GTCTTTACCA TGCCCTTGGC CACGCCCCTC TTTAATACGA CGGGCAATTT
  841 GCACTTCAGA AAATGAAAGA GTTTGCTTTA GCCATAACAA AGGTCCAGTA TGCTTTTTCA
  901 CAGCATACTG GGACTGATTT TCAGTTTAAC AAACTATTCT TGTCTAGTTT AAGGAACTTA
  961 ATTGTCAATA GTTAGAATCC TAATTTTGTT CAGTTAAAGA CTTTTAATGT TCGCTCAACA
 1021 TCCCGGCGTA CGCAGGGCAA TCCAATTTAA TAACCTCAAA CCGGTAAACG ATAGGCCCAA
1081 GTAACGCGCT GTTCAATGCG GTGTGAAAAA TACCGGCTCA CAGAGAC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yamada, T., Constitutive aryl hydrocarbon receptor signaling constrains type I interferon-mediated antiviral innate defense. Nat. Immunol. 17 (6): 687-694 (2016). PMID 27089381. [link to RRC of NBRP]