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pFastBac1-GST-hTIPARP(WT) (#RDB14327)

Baculovirus expression vector of GST tagged human TIPARP, wild type.

Clone info. Baculovirus expression vector of GST tagged human TCDD inducible poly (ADP-ribose) polymerase (TIPARP); wild type.
Comment Expression was confirmed by the deposotor with autoradiographic analysis and immunoblot.
Vector backbone pFastBac1 (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name human TIPARP cDNA
Depositor|Developer Takaoka, Akinori |
 

Distribution information

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Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Yamada, T. et al., Nat. Immunol. 17 (6): 687-694, 2016)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yamada, T. et al., Nat. Immunol. 17 (6): 687-694, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14327 pFastBac1-GST-hTIPARP(WT) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pFastBac1-GST-hTIPARP(WT) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14327).

Reference section:

Yamada, T., Horimoto, H., Kameyama, T., Hayakawa, S., Yamato, H., Dazai, M., Takada, A., Kida, H., Bott, D., Zhou, A.C., Hutin, D., Watts, T.H., Asaka, M., Matthews, J., Takaoka, A., Constitutive aryl hydrocarbon receptor signaling constrains type I interferon-mediated antiviral innate defense. Nat. Immunol. 17 (6): 687-694 (2016). PMID 27089381. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14327_A6I5p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pPolyhedrin_F (Pr0236)
Region: polyhedrin pro,GST,HRV 3C site
Sequence file: RDB14327_A6I5a.seq check
>D03656A5_A6I5_1_pPolyhedrin_F_H05_23_ABI24.ab1
    1 ATTTTACTGT TTTCGTACAG TTTTGTAATA AAAAAACCTA TAAATATTCC GGATTATTCA
   61 TACCGTCCCA CCATCGGGCG CGGATCTCCA CCATGTCCCC TATACTAGGT TATTGGAAAA
  121 TTAAGGGCCT TGTGCAACCC ACTCGACTTC TTTTGGAATA TCTTGAAGAA AAATATGAAG
  181 AGCATTTGTA TGAGCGCGAT GAAGGTGATA AATGGCGAAA CAAAAAGTTT GAATTGGGTT
  241 TGGAGTTTCC CAATCTTCCT TATTATATTG ATGGTGATGT TAAATTAACA CAGTCTATGG
  301 CCATCATACG TTATATAGCT GACAAGCACA ACATGTTGGG TGGTTGTCCA AAAGAGCGTG
  361 CAGAGATTTC AATGCTTGAA GGAGCGGTTT TGGATATTAG ATACGGTGTT TCGAGAATTG
  421 CATATAGTAA AGACTTTGAA ACTCTCAAAG TTGATTTTCT TAGCAAGCTA CCTGAAATGC
  481 TGAAAATGTT CGAAGATCGT TTATGTCATA AAACATATTT AAATGGTGAT CATGTAACCC
  541 ATCCTGACTT CATGTTGTAT GACGCTCTTG ATGTTGTTTT ATACATGGAC CCAATGTGCC
  601 TGGATGCGTT CCCAAAATTA GTTTGTTTTA AAAAACGTAT TGAAGCTATC CCACAAATTG
  661 ATAAGTACTT GAAATCCAGC AAGTATATAG CATGGCCTTT GCAGGGCTGG CAAGCCACGT
  721 TTGGTGGTGG CGACCATCCT CCAAAATCGG ATCTGGAAGT TCTGTTCCAG GGGCCCCTGG
  781 GGATCCCCGG AATTCCCAAT ACTTATGGAA ATGGAAACCA CCGAACCTGA GCCAGACTGT
  841 GTAGTGCAGC CTCCCTCTCC TCCTGATGAC TTTTCATGCC AAATGAGACT CTCTGAGAAG
  901 ATCACTCCAT TGAAGACTTT GTTTTAAGAA AAAGGATCAG AAAAGATTGG GAACTGGAAC
  961 CCTGAGTCTT TGAGCCAATA TTAAACACTC TTCTAGAATC TGGCTCACTT GATGGGGGTT
 1021 TTTTAGAATC TAGACCAGAG TTACAGAATG AGACCAGCCT ACATGGACCT ATGATGAGAG
1081 CATTGCAATT CAATCATCAT GCCCCAACCA GGCAGGA
//
Primer: pGEX_F (Pr0076)
Region: HRV 3C site,insert5'
Sequence file: RDB14327_A6I5b.seq check
>D03656A5_A6I5_1_pGEX-F_F05_17_ABI24.ab1
    1 GTGCAAATCC CTCAAAATCG GGGATCTGGG AAGTTCTGTT CCAGGGGCCC CTGGGATCCC
   61 CGGAATTCCC AATACTTATG GAAATGGAAA CCACCGAACC TGAGCCAGAC TGTGTAGTGC
  121 AGCCTCCCTC TCCTCCTGAT GACTTTTCAT GCCAAATGAG ACTCTCTGAG AAGATCACTC
  181 CATTGAAGAC TTGTTTTAAG AAAAAGGATC AGAAAAGATT GGGAACTGGA ACCCTGAGGT
  241 CTTTGAGGCC AATATTAAAC ACTCTTCTAG AATCTGGCTC ACTTGATGGG GTTTTTAGAT
  301 CTAGGAACCA GAGTACAGAT GAGAACAGCT TACATGAACC TATGATGAAG AAAGCCATGG
  361 AAATCAATTC ATCATGCCCA CCAGCAGAAA ATAATATGTC TGTTCTGATT CCTGATAGGA
  421 CAAATGTTGG GGACCAGATA CCGGAAGCCC ATCCTTCCAC TGAAGCTCCA GAACGAGTGG
  481 TTCCAATCCA AGATCACAGC TTTCCATCAG AAACCCTCAG TGGGACGGTG GCAGATTCCA
  541 CACCAGCTCA CTTCCAGACT GATCTTTTGC ACCCAGTTTC AAGTGATGTT CCTACTAGTC
  601 CTGACTGCTT AGACAAAGTC ATAGATTATG TTCCAGGCAT TTTCCAAGAA AACAGTTTTA
  661 CAATCCAATA CATTCTGGAC ACCAGTGATA AGCTGAGTAC TGAGCTCTTT CAGGACAAAA
  721 GTGAAGAGGC TTCCCTTGAC CTCGTGTTTG AGCTGGTGAA CCAGTTGCAG TACCACACTC
  781 ACCAAGAGAA CGGAATTGAA ATTTGCATGG ACTTTCTGCA AGGCACTTGT ATTTATGGCA
  841 GGGATTGTTT GAAGCACCAC ACTGTCTTGC CATATCATTG GCAGATCAAA AGGGACAACT
  901 ACTCAAAAGT GGCAGAGTGT ATTCAATGAT TTCTCAGGAG CACTTGGAAA AGATTTTACT
  961 GTAACCCAGA AAATGATAGA AATGAGATGA AGTATGGGAG GACAAGAATT TTGGGGCAGA
 1021 TTTGGAATGC CAATGAACGT GTATGAAACA ACTGAATTTG AACCAAACTA CGAGCTGTTC
1081 AACCACCACT CCTAGCATGG TCAACTCTAT TTACGCACAC ACGACAAGTT CCTTTG
//
Primer: SV40polyA-R (Pr0109)
Region: insert3'
Sequence file: RDB14327_A6I5c.seq check
>D03656A5_A6I5_1_SV40polyA-R_G05_20_ABI24.ab1
    1 AAAAATTGGA GTTTTTTAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC TGATTATGAT
   61 CCTCTAGTAC TTCTCGACAA GCTTGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC
  121 GAGGCAGATC GTCAGTCAGT CACGATGCGG CCGCTCGAGT CGACCCGGGA ATTCCCAATA
  181 CTTCAAATGG AAACAGTGTT ACTGACTTCT TCATATTGGA TAACAAAATA AGGGTAACTC
  241 TGGTCATCAT TAAAAATGAC AAAAATCTGA GGCTCAAAGA AATTATCCAC ACAAGAGTCA
  301 TAAAGGTCAC TGGTGACACT GCCAGGATTG ACTGGCGGGG GCCTTCTCAT GCCATGACTG
  361 CCCATTGTGT ATCTGCCCGT CAGCACTTTG GCCAGAAACA TGAAGTGGAC TCCTTTGGAG
  421 GACTTCTTAG AAAAGTTATG AGAGTAGCTT GCCTTCTTTG CAAAATAACT GCCTTGTCCA
  481 AACATTGTAG CATGCTTTCC ACAGACTCGA GGGTCAAAGT TGTGTTTGCA GATTCCATCT
  541 ACCACATCCT GGGATGTTCC ATGAAATAAA TGTCTCTCAT TTATTATCCT GTCACGGCCA
  601 AACATTTTCC TGTTCATATA TTCCTTTTTC CTTTTATATT TCTCCCAAAG AAACTGGTTT
  661 TGGACTCTCA ATATCTGCAA AATTCTGTAT TTAAACTCAG GCACAGTCTT ATGAAAAAGA
  721 TTGTAAATGA TCCGATAACT TTTATCCTCT GCAGAAACAG GGACTTGGAT GAAGTCCTGA
  781 GATGGATGCA TATAAACCCA AGTTTCAGGG TAAAAGTTTG CTGAAGTGAC CCCATCTGGG
  841 CAGATAATTT GTGATGATGA AGTTGCTTCA AGAGGTGGAG GAGCTTGTGT GGGAACCCCA
  901 CCAAGTGTCT GTAAATATGG AAGCAGTATA AAACAGGAGC GGAAGAGGGG TCTCCTTTTT
  961 TATCTCTCTC CTGAAGAATG AATTGTGGGA GGATGTAGTG GTTTATTCCC ACATCATAAA
 1021 TCGAACCCTC TTTCAGACCC CGAGAGTTGG GCTTTCTTCA ATCCAATCGA TGACAGACCT
1081 CGGGGATACT CTCTCCATTC AAGTTGGTTC CCTACAGAGA GATTTCCAGA ACCTGT
//
Primer: f1ori_R3 (Pr0142)
Region: Tn7L,SV40p(A),insert3'
Sequence file: RDB14327_A6I5d.seq check
>D03656A5_A6I5_1_f1ori_R3_B11_05_ABI24.ab1
    1 TTCCCTGCAT TAGCTGCATA GCGTTGATAT TCAGTCAATT ACAAACATTA ATAACGAAGA
   61 GATGACAGAA AAATTTTCAT TCTGTGACAG AGAAAAAGTA GCCGAAGATG ACGGTTTGTC
  121 ACATGGAGTT GGCAGGATGT TTGATTAAAA ACATAACAGG AAGAAAAATG CCCCGCTGTG
  181 GGCGGACAAA ATAGTTGGGA ACTGGGAGGG GTGGAAATGG AGTTTTTAAG GATTATTTAG
  241 GGAAGAGTGA CAAAATAGAT GGGAACTGGG TGTAGCGTCG TAAGCTAATA CGAAAATTAA
  301 AAATGACAAA ATAGTTTGGA ACTAGATTTC ACTTATCTGG TTCGGATCTC CTAGGCTCAA
  361 GCAGTGATCA GATCCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG
  421 AATGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC
  481 CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT
  541 TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC
  601 TGATTATGAT CCTCTAGTAC TTCTCGACAA GCTTGTGTCA GAGGTTTTCA CCGTCATCAC
  661 CGAAACGCGC GAGGCAGATC GTCAGTCAGT CACGATGCGG CCGCTCGAGT CGACCCGGGA
  721 ATTCCCAATA CTTCAAATGG AAACAGTGTT ACTGACTTCT TCATATTGGA TAACAAAATA
  781 AGGGTAACTC TGGTCATCAT TAAAAATGAC AAAAATCTGA GGCTCAAAGA AATTATCCAC
  841 ACAAGAGTCA TAAAGGTCAC TGGTGACACT GCCAGGATTG ACTGGCGGGG GCCTTCTCAT
  901 GCCATGACTG CCCATTGTGT ATCTGCCCGT CAGCACTTTG GCCCAGAAAC ATGAAGTGGA
  961 CTCCCTTTGG GAGGACTTCT TTAGAAAAGT TATGAGAGTA GCTTTGCCTT CTTTTGCAAA
 1021 ATAACTGCCT TTGTCCAAAC ATTTGTAGCA TGCCTTTTCA CAGACTCGGA GGATCGAGTT
1081 GTGTTTGCCA GATTCATCTA ACCACATTCC CTGGGGGAAT GGATC
//
Primer: ColE1ori_F (Pr0393)
Region: ori,Tn7R
Sequence file: RDB14327_A6I5e.seq check
>D03656A5_A6I5_1_ColE1ori_F_C11_08_ABI24.ab1
    1 TAGTCCGCCT CTCACACTGT CAGTCGCACT CATGACTTGA GCGTCGATTT TTGTGATGCT
   61 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG
  121 CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA
  181 ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA
  241 GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC
  301 TGTGCGGTAT TTCACACCGC ATAGACCAGC CGCGTAACCT GGCAAAATCG GTTACGGTTG
  361 AGTAATAAAT GGATGCCCTG CGTAAGCGGG TGTGGGCGGA CAATAAAGTC TTAAACTGAA
  421 CAAAATAGAT CTAAACTATG ACAATAAAGT CTTAAACTAG ACAGAATAGT TGTAAACTGA
  481 AATCAGTCCA GTTATGCTGT GAAAAAGCAT ACTGGACTTT TGTTATGGCT AAAGCAAACT
  541 CTTCATTTTC TGAAGTGCAA ATTGCCCGTC GTATTAAAGA GGGGCGTGGC CAAGGGCATG
  601 GTAAAGACTA TATTCGCGGC GTTGTGACAA TTTACCGAAC AAACTCCGCG GCCGGGAAGC
  661 CGATCTCGGC TTGAACGAAT TGTTAGGTGG CGGTACTTGG GTCGATATCA AAAGTGCATC
  721 ACTTCTTCCC GTATGCCCAA ACTTTTGTAT AGAGAGGCCA CTGCGGGGAT CGTCACCGTA
  781 AATCTGCTTT GCCACGTAGA TCACATAAAG CACCAAGCGC GTTTGGCCCT CATGCCTTGA
  841 GGAGATTGGA TGAGCGCGGT GGGCAAATGC CCCTTGCCCT TCCGGTGCCT CGCCCGGAAA
  901 CCTGGCGAAG ATTCTATAGA ATAATAGGAT TCTTCCACTT ACGGCGGCTT GGCTTCAAAA
  961 CTTTGGGGGC AGTAAACCTG TAATGCACCG GCCGAGAAGC CGCCTCAAGA CAAACCCGGT
 1021 CTTTCTTTTG GGTCTCGAAA AGGCCAAGCC TATAGGTCGG CCTGAATTGG AAATTGGTTT
 1081 CTTTTAAACT AAACCTGGAA GCCACAAGAT TATTCTTCCT CCGGAATGGC GATTATAAAT
1141 TCCCGAGGAA CAAGAGATTT TCACGC
//
Primer: Pcpro_F (Pr0413)
Region: GmR
Sequence file: RDB14327_A6I5f.seq check
>D03656A5_A6I5_1_Pcpro_F_A01_01_ABI08.ab1
    1 CATCGGTTGG CCGCCTCCGG CAACTGGGTC CAGAACCTTG ACCGAACGGC AGCGGTGGTA
   61 ACGGCGCAGT GGCGGTTTTC ATGGCTTGTT ATGACTGTTT TTTTGTACAG TCTATGCCTC
  121 GGGCATCCAA GCAGCAAGCG CGTTACGCCG TGGGTCGATG TTTGATGTTA TGGAGCAGCA
  181 ACGATGTTAC GCAGCAGCAA CGATGTTACG CAGCAGGGCA GTCGCCCTAA AACAAAGTTA
  241 GGTGGCTCAA GTATGGGCAT CATTCGCACA TGTAGGCTCG GCCCTGACCA AGTCAAATCC
  301 ATGCGGGCTG CTCTTGATCT TTTCGGTCGT GAGTTCGGAG ACGTAGCCAC CTACTCCCAA
  361 CATCAGCCGG ACTCCGATTA CCTCGGGAAC TTGCTCCGTA GTAAGACATT CATCGCGCTT
  421 GCTGCCTTCG ACCAAGAAGC GGTTGTTGGC GCTCTCGCGG CTTACGTTCT GCCCAAGTTT
  481 GAGCAGCCGC GTAGTGAGAT CTATATCTAT GATCTCGCAG TCTCCGGCGA GCACCGGAGG
  541 CAGGGCATTG CCACCGCGCT CATCAATCTC CTCAAGCATG AGGCCAACGC GCTTGGTGCT
  601 TATGTGATCT ACGTGCAAGC AGATTACGGT GACGATCCCG CAGTGGCTCT CTATACAAAG
  661 TTGGGCATAC GGGAAGAAGT GATGCACTTT GATATCGACC CAAGTACCGC CACCTAACAA
  721 TTCGTTCAAG CCGAGATCGG CTTCCCGGGC CGCGGAGTTG TTCGGTAAAT TGTCACAACG
  781 CCGCGAATAT AGTCTTTACC ATGCCCTTGG CCACGCCCCT CTTTAATACG ACGGGCAATT
  841 TGCACTTCAG AAAATGGAAG AGTTTTGCTT TTAGCCATAA CAAAAGTCCA GTATGCTTTT
  901 TTTCACCAGC ATAAACTGGG ACCTGATTTT CAGATTTACA ACCTAATTCT GTCTAGTTTT
  961 AGACTTAATT GTCATAGTTT TAGAGATCCT ATTTTTGGTC AGGTTGAGAA CTTTAATTGT
 1021 CGGCCACACC CGGCTTACGC CAGGCATCCA ATTTATTAAC TACACGGTTA ACGGAATTTG
1081 GGCCCAAGGT AACCGCTGGC TCTTATGCGC GTGTGCACAT ATCGGACTAG A
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yamada, T., Constitutive aryl hydrocarbon receptor signaling constrains type I interferon-mediated antiviral innate defense. Nat. Immunol. 17 (6): 687-694 (2016). PMID 27089381. [link to RRC of NBRP]