RDB14326 - pCAG-YFP-hTIPARP

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pCAG-YFP-hTIPARP (#RDB14326)

Expression vector of YFP tagged human TIPARP, wild type.

Clone info.	Expression vector driven by the CAG promoter for YFP tagged human TCDD inducible poly (ADP-ribose) polymerase (TIPARP); wild type.
Comment	Expression was confirmed by the deposotor with fluorescent microscopic observation.
Vector backbone	pCAGGS-YFP (Plasmid)
Selectable markers	Amp^r
Gene/insert name	human TIPARP cDNA Aequorea victoria GFP cDNA
Depositor\|Developer	Takaoka, Akinori \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Yamada, T. et al., Nat. Immunol. 17 (6): 687-694, 2016)

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Yamada, T. et al., Nat. Immunol. 17 (6): 687-694, 2016)

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yamada, T. et al., Nat. Immunol. 17 (6): 687-694, 2016)。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14326	pCAG-YFP-hTIPARP	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAG-YFP-hTIPARP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14326).

Reference section:

Yamada, T., Horimoto, H., Kameyama, T., Hayakawa, S., Yamato, H., Dazai, M., Takada, A., Kida, H., Bott, D., Zhou, A.C., Hutin, D., Watts, T.H., Asaka, M., Matthews, J., Takaoka, A., Constitutive aryl hydrocarbon receptor signaling constrains type I interferon-mediated antiviral innate defense. Nat. Immunol. 17 (6): 687-694 (2016). PMID 27089381. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14326_A6I5p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB14326_A6I5a.seq
>D03656A4_A6I5_1_pAxCA_F1_B07_02_ABI08.ab1 1 GGAAGGGGAT GCCGCTACAT GTTCATGCCT TCTTCTTTTT CCTACAGCTC CTGGGCAACG 61 TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT CGCCAGGGTT TTCCCAGTCA 121 CGACGTTGTA AAACGACGGC CAGTGAGCGC GCGTAATACG ACTCACTATA GGGCGAATTG 181 GGTACCGGGC CCCCCCTCGA TCCGGCATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG 241 TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG 301 GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG 361 GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCTT CGGCTACGGC CTGAAGTGCT 421 TCGCCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG 481 GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG 541 AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA 601 AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT 661 ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC CGCCACAACA 721 TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG 781 GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCTACCA GTCCGCCCTG AGCAAAGACC 841 CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC 901 TCGGCATGGA CGAGCTGTAC AAGCTCGACG GTATCGATAA GCTTGATATC GAATTCCCAA 961 TACTTATGGG AAATGGAAAC CACCGAACCT GAGCCAGACT GTGTAGTGCA GCCTCCCTCT 1021 CCTCTGATGA CTTTTCATGC CAAATGAGAC TCTCTGGAGA AGATCACTCC ATGAGACTTG 1081 TTTTAGACAC GATCAGAAAA GATGGACCTG AACCTGAGTC TTGAAGCATA ATTAACACTC 1141 TCTAGATCTG CTCACTGAAT GGTTGATCAG GACAGAGTAC GAGGAGAACG GACCTAA //
Primer: pAxCA_R1 Sequence file: RDB14326_A6I5b.seq
>D03656A4_A6I5_1_EYFP-C_F_A11_02_ABI24.ab1 1 CCGTTACAGA CCCCACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC 61 CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGCTCGAC GGTATCGATA AGCTTGATAT 121 CGAATTCCCA ATACTTATGG AAATGGAAAC CACCGAACCT GAGCCAGACT GTGTAGTGCA 181 GCCTCCCTCT CCTCCTGATG ACTTTTCATG CCAAATGAGA CTCTCTGAGA AGATCACTCC 241 ATTGAAGACT TGTTTTAAGA AAAAGGATCA GAAAAGATTG GGAACTGGAA CCCTGAGGTC 301 TTTGAGGCCA ATATTAAACA CTCTTCTAGA ATCTGGCTCA CTTGATGGGG TTTTTAGATC 361 TAGGAACCAG AGTACAGATG AGAACAGCTT ACATGAACCT ATGATGAAGA AAGCCATGGA 421 AATCAATTCA TCATGCCCAC CAGCAGAAAA TAATATGTCT GTTCTGATTC CTGATAGGAC 481 AAATGTTGGG GACCAGATAC CGGAAGCCCA TCCTTCCACT GAAGCTCCAG AACGAGTGGT 541 TCCAATCCAA GATCACAGCT TTCCATCAGA AACCCTCAGT GGGACGGTGG CAGATTCCAC 601 ACCAGCTCAC TTCCAGACTG ATCTTTTGCA CCCAGTTTCA AGTGATGTTC CTACTAGTCC 661 TGACTGCTTA GACAAAGTCA TAGATTATGT TCCAGGCATT TTCCAAGAAA ACAGTTTTAC 721 AATCCAATAC ATTCTGGACA CCAGTGATAA GCTGAGTACT GAGCTCTTTC AGGACAAAAG 781 TGAAGAGGCT TCCCTTGACC TCGTGTTTGA GCTGGTGAAC CAGTTGCAGT ACCACACTCA 841 CCAAGAGAAC GGAATTGAAA TTTGCATGGA CTTTCTGCAA GGCACTTGTA TTTATGGCAG 901 GATTGTTTGA AGCACCACAC TGTCTTGCCA TATCATTGGC AGATCAAAAG GACAACTACT 961 CAAAAGTGGC AGAGTGTATT CAATGATTCT CAGAGCACTT GGAAAGATTT TTACTGTACC 1021 CAGAAAATGA TAGATGAAGA TGGAGGTATG AAGAACAAGG ATTTGGGCCA GATTTCAGTG 1081 CCATGACGTG TTATGAACCA ACCGTG //
Primer: EYFP-C_F Sequence file: RDB14326_A6I5c.seq
>D03656A4_A6I5_1_pAxCA_R1_C07_03_ABI08.ab1 1 GAGACGAGGG CCATTGGCCA CACCAGCCAC CACCTTCTGA TAGGCAGCCT GCACCTGAGG 61 AGTGAATTAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC 121 GCGCAATTAA CCCTCACTAA AGGGAACAAA AGCTGGAGCT CCACCGCGGT GGCGGCCGCT 181 CTAGAACTAG TGGATCCCCC GGGCTGCAGG AATTCCCAAT ACTTCAAATG GAAACAGTGT 241 TACTGACTTC TTCATATTGG ATAACAAAAT AAGGGTAACT CTGGTCATCA TTAAAAATGA 301 CAAAAATCTG AGGCTCAAAG AAATTATCCA CACAAGAGTC ATAAAGGTCA CTGGTGACAC 361 TGCCAGGATT GACTGGCGGG GGCCTTCTCA TGCCATGACT GCCCATTGTG TATCTGCCCG 421 TCAGCACTTT GGCCAGAAAC ATGAAGTGGA CTCCTTTGGA GGACTTCTTA GAAAAGTTAT 481 GAGAGTAGCT TGCCTTCTTT GCAAAATAAC TGCCTTGTCC AAACATTGTA GCATGCTTTC 541 CACAGACTCG AGGGTCAAAG TTGTGTTTGC AGATTCCATC TACCACATCC TGGGATGTTC 601 CATGAAATAA ATGTCTCTCA TTTATTATCC TGTCACGGCC AAACATTTTC CTGTTCATAT 661 ATTCCTTTTT CCTTTTATAT TTCTCCCAAA GAAACTGGTT TTGGACTCTC AATATCTGCA 721 AAATTCTGTA TTTAAACTCA GGCACAGTCT TATGAAAAAG ATTGTAAATG ATCCGATAAC 781 TTTTATCCTC TGCAGAAACA GGGACTTGGA TGAAGTCCTG AGATGGATGC ATATAAACCC 841 AAGTTTCAAG GTAAAAGTTT GCTGAAGTGA CCCCATCTGG GCAGATAATT TGTGATGATG 901 AGTTGCTTCA AGAGGTGGAG GAGCTTGTGT GGGAACCCCA CCAAGTGTCT GTAAATATGG 961 AAGCAGTATA AAAACAGGAG CGGAAGAGGG GTCTCTTTTT ATCTCTTCTC TGAAGATGAA 1021 TTGTGGAGGA TGTAGTGGTT ATCCACATCA TAAATCGGAA CTCTTTCAGA CCCGAGAAGT 1081 GCCTTCTTCA ATCCATCGAA TGACAGAACT CGGGATCCTC TCTCCATCGA AGTGTCCTAA 1141 CAGAAGATCC GGACTTGTGT GTGTAATAGT GACTTGCTAA GGGGGTGTGG ACAGCTTCTG 1201 AGTGATG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Yamada, T., Constitutive aryl hydrocarbon receptor signaling constrains type I interferon-mediated antiviral innate defense. Nat. Immunol. 17 (6): 687-694 (2016). PMID 27089381. [link to RRC of NBRP]