Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pIRM21-3HA-C-hTIPARP (#RDB14325)

Expression vector of HA tagged human TIPARP, deletion mutant of 1-236 AA.

Clone info. Expression vector driven by the CAG promoter for HA tagged human TCDD inducible poly (ADP-ribose) polymerase (TIPARP) mutant; deletion of 1-236 AA. See Fig. S4e of Yamada, T., Nat. Immunol, 17 (6): 687-694, 2016 for details.
Comment Expression was confirmed by the deposotor with immunoprecipitation and immunoblotting.
Vector backbone pIRM-3HA (Plasmid)
Selectable markers Amp^r
Gene/insert name human TIPARP cDNA Discosoma sp. RFP cDNA
Depositor|Developer Takaoka, Akinori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Yamada, T. et al., Nat. Immunol. 17 (6): 687-694, 2016)
Additional terms and conditions:
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
Remarks Please contact us. We will provide you with appropriate documents.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yamada, T. et al., Nat. Immunol. 17 (6): 687-694, 2016)。
MTAに書く付加的使用条件：
非営利機関の非営利学術研究に限って提供する。
備考 クローンに使用している蛍光タンパク質等により、書式の様式が異なります。お問い合わせください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14325 pIRM21-3HA-C-hTIPARP DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pIRM21-3HA-C-hTIPARP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14325).

Reference section:

Yamada, T., Horimoto, H., Kameyama, T., Hayakawa, S., Yamato, H., Dazai, M., Takada, A., Kida, H., Bott, D., Zhou, A.C., Hutin, D., Watts, T.H., Asaka, M., Matthews, J., Takaoka, A., Constitutive aryl hydrocarbon receptor signaling constrains type I interferon-mediated antiviral innate defense. Nat. Immunol. 17 (6): 687-694 (2016). PMID 27089381. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14325_A6I5p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB14325_A6I5a.seq check
>D03656A3_A6I5_1_pAxCA_F1_F03_18_ABI24.ab1
    1 GTTGGCGATC TGCTACATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT GGGCAACGTG
   61 CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTGC CATGGCATAC CCTTATGACG
  121 TACCAGATTA CGCGGGATAT CCCTACGATG TCCCCGATTA CGCGCGTTAT CCATATGACG
  181 TCCCGGACTA TGCCGAATTC GTGATTCTCG ACGAGAACGG AATTGAAATT TGCATGGACT
  241 TTCTGCAAGG CACTTGTATT TATGGCAGGG ATTGTTTGAA GCACCACACT GTCTTGCCAT
  301 ATCATTGGCA GATCAAAAGG ACAACTACTC AAAAGTGGCA GAGTGTATTC AATGATTCTC
  361 AGGAGCACTT GGAAAGATTT TACTGTAACC CAGAAAATGA TAGAATGAGA ATGAAGTATG
  421 GAGGACAAGA ATTTTGGGCA GATTTGAATG CCATGAACGT GTATGAAACA ACTGAATTTG
  481 ACCAACTACG AAGGCTGTCC ACACCACCCT CTAGCAATGT CAACTCTATT TACCACACAG
  541 TCTGGAAATT CTTCTGTAGG GACCACTTTG GATGGAGAGA GTATCCCGAG TCTGTCATTC
  601 GATTGATTGA AGAAGCCAAC TCTCGGGGTC TGAAAGAGGT TCGATTTATG ATGTGGAATA
  661 ACCACTACAT CCTCCACAAT TCATTCTTCA GGAGAGAGAT AAAAAGGAGA CCCCTCTTCC
  721 GCTCCTGTTT TATACTGCTT CCATATTTAC AGACACTTGG TGGGGTTCCC ACACAAGCTC
  781 CTCCACCTCT TGAAGCAACT TCATCATCAC AAATTATCTG CCCAGATGGG GTCACTTCAG
  841 CAAACTTTTA CCCTGAAACT TGGGTTTATA TGCATCCATC TCAGGACTTC ATCCAAGTCC
  901 CTGTTTCTGC AGAGGATAAA AGTTATCGGA TCATTTACAA TCTTTTTCAT AAGACTGTGC
  961 CTGAGTTTAA ATACAGAATT TTGCAGATAT TGAGAGTCCA AAACCCAGTT TTCTTTGGGA
 1021 GAAATAATAA AAGAAAATGA ATATATGACA GGAAATGTTG ATCGTGACAG ATATTATGCA
1081 GGACAGTTAC TTCATGCACC ATTCCCAAGG CATGGGTGGC TAG
//
Primer: IRES-R
Sequence file: RDB14325_A6I5b.seq check
>D03656A3_A6I5_1_IRES-R_G03_21_ABI24.ab1
    1 CCTAGCGGGC TTCGGCCAGT ACGTTAGGGG GGGGCGGAAT TTACGTAGCG GCCCGATCGT
   61 CAGTCAGTCA CGATGCGGCC GCTCAAGAAC TAGTGGATCC CCCGGGCTGC AGGAATTCCC
  121 AATACTTCAA ATGGAAACAG TGTTACTGAC TTCTTCATAT TGGATAACAA AATAAGGGTA
  181 ACTCTGGTCA TCATTAAAAA TGACAAAAAT CTGAGGCTCA AAGAAATTAT CCACACAAGA
  241 GTCATAAAGG TCACTGGTGA CACTGCCAGG ATTGACTGGC GGGGGCCTTC TCATGCCATG
  301 ACTGCCCATT GTGTATCTGC CCGTCAGCAC TTTGGCCAGA AACATGAAGT GGACTCCTTT
  361 GGAGGACTTC TTAGAAAAGT TATGAGAGTA GCTTGCCTTC TTTGCAAAAT AACTGCCTTG
  421 TCCAAACATT GTAGCATGCT TTCCACAGAC TCGAGGGTCA AAGTTGTGTT TGCAGATTCC
  481 ATCTACCACA TCCTGGGATG TTCCATGAAA TAAATGTCTC TCATTTATTA TCCTGTCACG
  541 GCCAAACATT TTCCTGTTCA TATATTCCTT TTTCCTTTTA TATTTCTCCC AAAGAAACTG
  601 GTTTTGGACT CTCAATATCT GCAAAATTCT GTATTTAAAC TCAGGCACAG TCTTATGAAA
  661 AAGATTGTAA ATGATCCGAT AACTTTTATC CTCTGCAGAA ACAGGGACTT GGATGAAGTC
  721 CTGAGATGGA TGCATATAAA CCCAAGTTTC AGGGTAAAAG TTTGCTGAAG TGACCCCATC
  781 TGGGCAGATA ATTTGTGATG ATGAAGTTGC TTCAAGAGGT GGAGGAGCTT GTGTGGGAAC
  841 CCCACCAAGT GTCTGTAAAT ATGGAAGCAG TATAAAACAG GAGCGGAAGA GGGTCTCCTT
  901 TTTATCTCTC TCCTGAAGAA TGAATTGTGG AGGATGTAGT GGTTATTCCA CATCATAAAT
  961 CGAACCTCTT TCAGACCCCG AGAGTTGGCT TCTTTCAATC AATCGAATGA CAGACTCGGG
 1021 GATACTCTCT CCATCCAAAG TGGTCCCCTA CAGAGGATTT TCAGACCTGT GGTGGTAAAT
 1081 AGAGTTTGAC ATGGCTTAGA GGGTTGGTGT GAACAGCTTC GTAGTTTGGG TCAAAATTCC
1141 AGT
//
Primer: IRES-F
Sequence file: RDB14325_A6I5c.seq check
>D03656A3_A6I5_1_IRES-F_H03_24_ABI24.ab1
    1 TCATCTCTGT GTTTAGTCGA GGTTAAAAAA CGTCTAGGCC CCCCGAACCA CGGGGACGTG
   61 GTTTTCCTTT GAAAAACACG ATGATAATAT GGCCACAACC ATGAGCTGTA GCAAGAACGT
  121 GATCAAGGAG TTCATGCGCT TCAAGGTGCG CATGGAGGGC ACCGTGAACG GCCACGAGTT
  181 CGAGATCAAG GGCGAGGGCG AAGGCCGCCC CTACGAGGGC CACTGTAGCG TGAAGCTGAT
  241 GGTGACCAAG GGCGGCCCCC TGCCCTTCGC CTTCGACATC CTGAGCCCCC AGTTCCAGTA
  301 CGGCAGCAAG GTGTACGTGA AGCACCCCGC CGACATCCCC GACTACCTGA AGCTGAGCTT
  361 CCCCGAGGGC TTCAAGTGGG AGCGCGTGAT GAACTTCGAG GACGGCGGCG TGGTGACCGT
  421 GAGCCAGGAT TCGAGCCTGA AGGACGGCTG CTTCATCTAC GAGGTGAAGT TCATCGGCGT
  481 GAACTTCCCC AGCGACGGCC CCGTGATGCA GCGCCGCACC CGCGGCTGGG AGGCCAGCAG
  541 CGAGCGCCTG TACCCCCGCG ACGGCGTGCT GAAGGGCGAC ATCCACATGG CCCTGCGCCT
  601 GGAGGGCGGC GGCCACTACC TGGTGGAGTT CAAGAGCATC TACATGGTGA AGAAGCCCAG
  661 CGTGCAACTG CCCGGCTACT ACTACGTGGA CAGCAAGCTG GACATGACCA GCCACAACGA
  721 GGACTACACC GTGGTGGAGC AGTACGAGAA GACCCAGGGC CGCCTCCACC CCTTCATCAA
  781 GCACCTACAG TGAGATCTTT TTCCCTCTGC CAAAAATTAT GGGGACATCA TGAAGCCCCT
  841 TGAGCATCTG ACTTCTGGCT AATAAAGGAA ATTTATTTTC ATTGCAATAG TGTGTTGGAA
  901 ATTTTTTGTG TCTCTCACTC GGAAGGACAT ATGGGAGGGC AAATCATTTA AAACATCAGA
  961 ATGAGTATTT GGGTTTAGAG TTTGGCACAT ATGCCCATAT GCTGGGCTGC ATGACACGTT
 1021 GGGCTATAAA GAGTCATCAG TATATGAAAC AGCCCCTGCT GTCAATCGTA TCGATAGAAA
1081 GCCGTTGACT TGAGTTAGAC TTTTTAAAAT TTTG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yamada, T., Constitutive aryl hydrocarbon receptor signaling constrains type I interferon-mediated antiviral innate defense. Nat. Immunol. 17 (6): 687-694 (2016). PMID 27089381. [link to RRC of NBRP]