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pIRM21-3HA-hTIPARP(WT) (#RDB14323)

Expression vector of HA tagged human TIPARP wild type.

Clone info. Expression vector driven by the CAG promoter for HA tagged human TCDD inducible poly (ADP-ribose) polymerase (TIPARP); wild type. See Fig. S4e of Yamada, T., Nat. Immunol, 17 (6): 687-694, 2016 for details.
Comment Expression was confirmed by the deposotor with immunoprecipitation and immunoblotting.
Vector backbone pIRM-3HA (Plasmid)
Selectable markers Amp^r
Gene/insert name human TIPARP cDNA Discosoma sp. RFP cDNA
Depositor|Developer Takaoka, Akinori |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Yamada, T. et al., Nat. Immunol. 17 (6): 687-694, 2016)
Additional terms and conditions:
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
Remarks Please contact us. We will provide you with appropriate documents.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yamada, T. et al., Nat. Immunol. 17 (6): 687-694, 2016)。
MTAに書く付加的使用条件：
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14323 pIRM21-3HA-hTIPARP(WT) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pIRM21-3HA-hTIPARP(WT) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14323).

Reference section:

Yamada, T., Horimoto, H., Kameyama, T., Hayakawa, S., Yamato, H., Dazai, M., Takada, A., Kida, H., Bott, D., Zhou, A.C., Hutin, D., Watts, T.H., Asaka, M., Matthews, J., Takaoka, A., Constitutive aryl hydrocarbon receptor signaling constrains type I interferon-mediated antiviral innate defense. Nat. Immunol. 17 (6): 687-694 (2016). PMID 27089381. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14323_A6I5p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB14323_A6I5a.seq check
>D03656A1_A6I5_1_pAxCA_F1_A02_02_ABI24.ab1
    1 GGTAGCGATC TGCTACATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT GGGCAACGTG
   61 CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTGC CATGGCATAC CCTTATGACG
  121 TACCAGATTA CGCGGGATAT CCCTACGATG TCCCCGATTA CGCGCGTTAT CCATATGACG
  181 TCCCGGACTA TGCCGAATTC GTGATTCTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCC
  241 CAATACTTAT GGAAATGGAA ACCACCGAAC CTGAGCCAGA CTGTGTAGTG CAGCCTCCCT
  301 CTCCTCCTGA TGACTTTTCA TGCCAAATGA GACTCTCTGA GAAGATCACT CCATTGAAGA
  361 CTTGTTTTAA GAAAAAGGAT CAGAAAAGAT TGGGAACTGG AACCCTGAGG TCTTTGAGGC
  421 CAATATTAAA CACTCTTCTA GAATCTGGCT CACTTGATGG GGTTTTTAGA TCTAGGAACC
  481 AGAGTACAGA TGAGAACAGC TTACATGAAC CTATGATGAA GAAAGCCATG GAAATCAATT
  541 CATCATGCCC ACCAGCAGAA AATAATATGT CTGTTCTGAT TCCTGATAGG ACAAATGTTG
  601 GGGACCAGAT ACCGGAAGCC CATCCTTCCA CTGAAGCTCC AGAACGAGTG GTTCCAATCC
  661 AAGATCACAG CTTTCCATCA GAAACCCTCA GTGGGACGGT GGCAGATTCC ACACCAGCTC
  721 ACTTCCAGAC TGATCTTTTG CACCCAGTTT CAAGTGATGT TCCTACTAGT CCTGACTGCT
  781 TAGACAAAGT CATAGATTAT GTTCCAGGCA TTTTCCAAGA AAACAGTTTT ACAATCCAAT
  841 ACATTCTGGA CACCAGTGAT AAGCTGAGTA CTGAGCTCTT TCAGGACAAA AGTGAAGAGG
  901 CTTCCCTTGA CCTCGTGTTT GAGCTGGTGA ACCAGTTGCA GTACCACACT CACCAAGAGA
  961 ACGGAATTGA AATTTGCATG GACTTTTCTG CAAGGCACTT GTATTTTATG GGCAGGATTG
 1021 TTTGAAGGCA CACACTGTCC TTGGCATATC ATGCAGATCA AAACGACACT ACTCAAAAGT
1081 GGCAGATGCA ATCAATGATC TCAGGGAGCA CTTCAAAGGA TTTTTACCTG TGTAAC
//
Primer: IRES-R
Sequence file: RDB14323_A6I5b.seq check
>D03656A1_A6I5_1_IRES-R_A08_01_ABI08.ab1
    1 GCATCAGCGG CTTCGGCCAG TACGTTAGGG GGGGGCGGAA TTTACGTAGC GGCCCGATCG
   61 TCAGTCAGTC ACGATGCGGC CGCTCTAGAA CTAGTGGATC CCCCGGGCTG CAGGAATTCC
  121 CAATACTTCA AATGGAAACA GTGTTACTGA CTTCTTCATA TTGGATAACA AAATAAGGGT
  181 AACTCTGGTC ATCATTAAAA ATGACAAAAA TCTGAGGCTC AAAGAAATTA TCCACACAAG
  241 AGTCATAAAG GTCACTGGTG ACACTGCCAG GATTGACTGG CGGGGGCCTT CTCATGCCAT
  301 GACTGCCCAT TGTGTATCTG CCCGTCAGCA CTTTGGCCAG AAACATGAAG TGGACTCCTT
  361 TGGAGGACTT CTTAGAAAAG TTATGAGAGT AGCTTGCCTT CTTTGCAAAA TAACTGCCTT
  421 GTCCAAACAT TGTAGCATGC TTTCCACAGA CTCGAGGGTC AAAGTTGTGT TTGCAGATTC
  481 CATCTACCAC ATCCTGGGAT GTTCCATGAA ATAAATGTCT CTCATTTATT ATCCTGTCAC
  541 GGCCAAACAT TTTCCTGTTC ATATATTCCT TTTTCCTTTT ATATTTCTCC CAAAGAAACT
  601 GGTTTTGGAC TCTCAATATC TGCAAAATTC TGTATTTAAA CTCAGGCACA GTCTTATGAA
  661 AAAGATTGTA AATGATCCGA TAACTTTTAT CCTCTGCAGA AACAGGGACT TGGATGAAGT
  721 CCTGAGATGG ATGCATATAA ACCCAAGTTT CAGGGTAAAA GTTTGCTGAA GTGACCCCAT
  781 CTGGGCAGAT AATTTGTGAT GATGAAGTTG CTTCAAGAGG TGGAGGAGCT TGTGTGGGAA
  841 CCCCACCAAG TGTCTGTAAA TATGGAAGCA GTATAAAACA GGAGCGGAAG AGGGGTCTCC
  901 TTTTTATCTC TCTCCTGAAG AATGAATTGT GGAGGATGTA GTGGTTATTC CACATCATAA
  961 ATCGAACCTC TTTCAGACCC CGAGAGTTGG CTTCTTCAAT CAATCGAATG ACAGACTCGG
 1021 GATACTCTCT CCATCCAAAG TGTCCCTACA GAAGAATTTC AGACTGGGGT GGTAAATAGA
 1081 GTGACATGCT AGAGGGGTGG GTGTGGACAG CTCGTAGTGG TCAAATCAGT TGTTTCATAC
 1141 ACGTCATGGC AATCCAAATC TGACTAATCT TGTCTCGATA CCTTCAATTC TCAATTCTAT
1201 CAAATC
//
Primer: IRES-F
Sequence file: RDB14323_A6I5c.seq check
>D03656A1_A6I5_1_IRES-F_C02_08_ABI24.ab1
    1 GGAGGTCCTT TTCATGGGTT TAGTCGAGGT TAAAAAACGT CTAGGCCCCC CGAACCACGG
   61 GGACGTGGTT TTCCTTTGAA AAACACGATG ATAATATGGC CACAACCATG AGCTGTAGCA
  121 AGAACGTGAT CAAGGAGTTC ATGCGCTTCA AGGTGCGCAT GGAGGGCACC GTGAACGGCC
  181 ACGAGTTCGA GATCAAGGGC GAGGGCGAAG GCCGCCCCTA CGAGGGCCAC TGTAGCGTGA
  241 AGCTGATGGT GACCAAGGGC GGCCCCCTGC CCTTCGCCTT CGACATCCTG AGCCCCCAGT
  301 TCCAGTACGG CAGCAAGGTG TACGTGAAGC ACCCCGCCGA CATCCCCGAC TACCTGAAGC
  361 TGAGCTTCCC CGAGGGCTTC AAGTGGGAGC GCGTGATGAA CTTCGAGGAC GGCGGCGTGG
  421 TGACCGTGAG CCAGGATTCG AGCCTGAAGG ACGGCTGCTT CATCTACGAG GTGAAGTTCA
  481 TCGGCGTGAA CTTCCCCAGC GACGGCCCCG TGATGCAGCG CCGCACCCGC GGCTGGGAGG
  541 CCAGCAGCGA GCGCCTGTAC CCCCGCGACG GCGTGCTGAA GGGCGACATC CACATGGCCC
  601 TGCGCCTGGA GGGCGGCGGC CACTACCTGG TGGAGTTCAA GAGCATCTAC ATGGTGAAGA
  661 AGCCCAGCGT GCAACTGCCC GGCTACTACT ACGTGGACAG CAAGCTGGAC ATGACCAGCC
  721 ACAACGAGGA CTACACCGTG GTGGAGCAGT ACGAGAAGAC CCAGGGCCGC CTCCACCCCT
  781 TCATCAAGCA CCTACAGTGA GATCTTTTTC CCTCTGCCAA AAATTATGGG GGACATCATG
  841 AAGCCCCTTG AGCATCTGAC TTCTGGCTAA TAAAGGAAAT TTATTTTCAT TGCAATAGTG
  901 TGTTGGAATT TTTTTGTGTC TCTCACTCGG AAGGACATAT GGGAGGGGCA AATCATTTAA
  961 AACATCAGAA ATGAGTATTT GGGTTTAGAG TTTGGGCAAC ATATGCCCAT ATGCTGGGCT
 1021 GCCATGAAAC AAGGGTTGGG CTATAAGAGG TCATCAGTAT ATGAACAGCC CCTGCTGTTC
1081 AATTCCTATT CATAGAAAAA GCTGGACTTG AAGGGTAGAT TTTTTTTTAA ATACTCTG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yamada, T., Constitutive aryl hydrocarbon receptor signaling constrains type I interferon-mediated antiviral innate defense. Nat. Immunol. 17 (6): 687-694 (2016). PMID 27089381. [link to RRC of NBRP]