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pMOE-mCherry-ble (#RDB14303)

Expression vector of mCherry-ble under the control of Perkinsus marinus MOE gene promoter.

Alternative name pPmMOE-mCherry-ble
Clone info. Expression vector of mCherry fused Sh-ble is controled under the Perkinsus marinus MOE (PmMOE, putative cell-wall protein) promoter, see Fig. 1 of Sakamoto, H. Parasitol. Int. 65 (5 Pt B): 563-566, 2016.
Comment This clone is for a drug selection system using a bleomycin for transfecting Perkinsus species. Perkinsus is sensitive to bleomycin, and 100 microgram/ml of this drug completely blocks its proliferation. Expression was confirmed by the depositor with fluorescence microscopy and flowcytometry. Refer DDBJ/ENA/GenBank, accession EF632302 for Perkinsus marinus strain CB5D4 putative cell-wall protein (MOE) gene and AAXJ01000000 for Perkinsus marinus genome sequence, respectively. DNA fragments of 1-1,098 and 1,507-2,638 of EF632302.1 are cloned. Known differences: substitutions of G to A at 302nt and A to T at 2,240nt, respectively.
Vector backbone pCR4-TOPO (Plasmid)
Size of vector backbone 4 kb
Selectable markers Amp^r, Kan^r.
Gene/insert name Perkinsus marinus MOE genomic DNA Discoma sp. RFP cDNA Streptoalloteichus hindustanus bleomycin resistance gene genomic DNA
Depositor|Developer Watanabe, Yo-ichi | Matsuzaki, Motomichi |
 
Sequence RDB14303z.seq

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Sakamoto, H. et al., Parasitol. Int. 65 (5 Pt B): 563-566, 2016) and an acknowledgment to the DEPOSITOR are requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Sakamoto, H. et al., Parasitol. Int. 65 (5 Pt B): 563-566, 2016)を引用し、謝辞の表明を必要とする。

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RDB14303 pMOE-mCherry-ble DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMOE-mCherry-ble was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14303).

Reference section:

Sakamoto, H., Kita, K., Matsuzaki, M., Drug selection using bleomycin for transfection of the oyster-infecting parasite Perkinsus marinus. Parasitol. Int. 65 (5 Pt B): 563-566 (2016). PMID 27094226. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14303_A6Hup1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pMOE-2 ()
Sequence file: RDB14303_A6Hua.seq check
>14303_14303_A8J9_pMOE-2_E04_13_ABI24
    1 GKASKCGTMT CTTGATCTCT ATCCCGCTGT TCAACGCAAT CATGAATCAC AAAGTGCATA
   61 TGGAGCTCGG ATCCGTGAGC AAGGGCGAGG AGGATAACAT GGCCATCATC AAGGAGTTCA
  121 TGCGCTTCAA GGTGCACATG GAGGGCTCCG TGAACGGCCA CGAGTTCGAG ATCGAGGGCG
  181 AGGGCGAGGG CCGCCCCTAC GAGGGCACCC AGACCGCCAA GCTGAAGGTG ACCAAGGGTG
  241 GCCCCCTGCC CTTCGCCTGG GACATCCTGT CCCCTCAGTT CATGTACGGC TCCAAGGCCT
  301 ACGTGAAGCA CCCCGCCGAC ATCCCCGACT ACTTGAAGCT GTCCTTCCCC GAGGGCTTCA
  361 AGTGGGAGCG CGTGATGAAC TTCGAGGACG GCGGCGTGGT GACCGTGACC CAGGACTCCT
  421 CCCTGCAGGA CGGCGAGTTC ATCTACAAGG TGAAGCTGCG CGGCACCAAC TTCCCCTCCG
  481 ACGGCCCCGT AATGCAGAAG AAGACCATGG GCTGGGAGGC CTCCTCCGAG CGGATGTACC
  541 CCGAGGACGG CGCCCTGAAG GGCGAGATCA AGCAGAGGCT GAAGCTGAAG GACGGCGGCC
  601 ACTACGACGC TGAGGTCAAG ACCACCTACA AGGCCAAGAA GCCCGTGCAG CTGCCCGGCG
  661 CCTACAACGT CAACATCAAG TTGGACATCA CCTCCCACAA CGAGGACTAC ACCATCGTGG
  721 AACAGTACGA ACGCGCCGAG GGCCGCCACT CCACCGGCGG CATGGACGAG CTGTACAAGG
  781 GAGGTGGATC AATGGCCAAG TTGACCAGTG CCGTTCCGGT GCTCACCGCG CGCGACGTCG
  841 CCGGAGCGGT CGAGTTCTGG ACCGACCGGC TCGGGTTCTC CCGGGACTTC GTGGAGGACG
  901 ACTTCGCCGG TGTGGTCCGG GACGACGTGA CCCTGTTCAT CAGCGCGGTC CAGGACCAGG
  961 TGGTGCCGGA CAACACCCTG GCCTGGGTGT GGGTGCGCGG CCTGGACGAG CTGTACGCCG
 1021 AGTGGTCGGA GGTCGTGTCC ACGAACTTCT GGACGCTCCT GGCCGGCCAT GAACGAGATC
 1081 GCGAGCAGCC GTGCGGACCG AGTCGACTTG CGCGAACCGA TCGCAACTGG CGKTGCAMCT
1141 TCCGTGTGGT CA
//
Primer: moe3_055R ()
Sequence file: RDB14303_A6Hub.seq check
>14303_14303_A8J9_moe3_055R_F04_16_ABI24
    1 TWKGGGKKAT TTCGACATTG ATGTAGATCT CAACTAGATG TGTTGTCTTA TCAGTCCTGC
   61 TCCTCGGCCA CGAAGTGCAC GCAGTTGCCG GCCGGGTCGC GCAGGGCGAA CTCCCGCCCC
  121 CACGGCTGCT CGCCGATCTC GGTCATGGCC GGCCCGGAGG CGTCCCGGAA GTTCGTGGAC
  181 ACGACCTCCG ACCACTCGGC GTACAGCTCG TCCAGGCCGC GCACCCACAC CCAGGCCAGG
  241 GTGTTGTCCG GCACCACCTG GTCCTGGACC GCGCTGATGA ACAGGGTCAC GTCGTCCCGG
  301 ACCACACCGG CGAAGTCGTC CTCCACGAAG TCCCGGGAGA ACCCGAGCCG GTCGGTCCAG
  361 AACTCGACCG CTCCGGCGAC GTCGCGCGCG GTGAGCACCG GAACGGCACT GGTCAACTTG
  421 GCCATTGATC CACCTCCCTT GTACAGCTCG TCCATGCCGC CGGTGGAGTG GCGGCCCTCG
  481 GCGCGTTCGT ACTGTTCCAC GATGGTGTAG TCCTCGTTGT GGGAGGTGAT GTCCAACTTG
  541 ATGTTGACGT TGTAGGCGCC GGGCAGCTGC ACGGGCTTCT TGGCCTTGTA GGTGGTCTTG
  601 ACCTCAGCGT CGTAGTGGCC GCCGTCCTTC AGCTTCAGCC TCTGCTTGAT CTCGCCCTTC
  661 AGGGCGCCGT CCTCGGGGTA CATCCGCTCG GAGGAGGCCT CCCAGCCCAT GGTCTTCTTC
  721 TGCATTACGG GGCCGTCGGA GGGGAAGTTG GTGCCGCGCA GCTTCACCTT GTAGATGAAC
  781 TCGCCGTCCT GCAGGGAGGA GTCCTGGGTC ACGGTCACCA CGCCGCCGTC CTCGAAGTTC
  841 ATCACGCGCT CCCACTTGAA GCCCTCGGGG AAGGACAGCT TCAAGTAGTC GGGGATGTCG
  901 GCGGGGTGCT TCACGTAGGC CTTGGAGCCG TACATGAACT GAGGGGACAG GATGTCCCAG
  961 GCGAAAGGGC AGGGGCCACC CTTGGTCACC TTCAGCTTGG CGGTCTGGGT GCCCTCGTAG
 1021 GGGCGGCCTC GCCCTCGCCC TCGATCTCGA ACTCGTGGCC GTTCACGGAG CCCTTCATGT
 1081 GCACATGAGC GCATGAACTC ATGATGATGC ATGTTATCCT CTCGCCTTGC TCCMCGGATC
1141 CGAAGCCTYC ATATG
//
Primer: M13R
Sequence file: RDB14303_A6Huc.seq check
>D03684A1_A6Hu_1_M13R_A01_01_ABI24.ab1
    1 AACCTAAAAT TACCTCACTA AAGGGACTAG TCCTGCAGGT TTAAACGAAT TCGCCCTTCT
   61 CGTAATGAGC CCAACCATTA TCGGATCGGT CTTGCGGGCG AACGAACATC TGCGGCAGGT
  121 GAGAGGTGGG AGTTTCCGGA TGCGGTCCGA GTCATCGGCG ATGGAACTCC CCACAGGCCA
  181 GTGTGACGGT GAGCACTGAT GTTCGATGGA GACCAAAGTG GCAGGGTTCG TACTTCCCAG
  241 CCATGGTTGC GCCGTTTGTA ATTGATGTGA TGGCAGAGCT GACCGGTAGC AGTAGAAGGT
  301 GCGTTGATTC AGCTCAACAG CTCTCTTGTG TTAGCAGAAA GTAGAAGCAG ATCTAGATGA
  361 TTCAGGAATC CCTCATCTTA GCGGTAGTAT GTTACTGTGC ACCCATCATG CCATGGATGA
  421 TCCGGATCCC TTGTTGGGTG AGGTAGGTCC CTTGGTGAGT TGGATGATTG TGGATCTGCC
  481 GATGGCAGAG ATCATGGCTC CATTCCCCAT CGGGACCTCA CGGTATCACT GATCATTTCA
  541 TGGCTCAAGG GCGGGCTTGT CGACTTCGAC AAGTGGGTGG GACTGCGGTG AGCAAGGTGC
  601 TTTGCACTTG CCGAACCCAT CGCTTTGCTC CGGCTTGTAG TGAACTAACC GGAGCAAGAA
  661 TAGGGCCCGG GCTAGTGGGA CGCAACACAT GAGGAACGTG TCTCAAATCC ATAGATTTTA
  721 TGTTGGATTC GTCTCGTCGG AGCCAAGTGC TCCACGATGT GCATCCCTCG AGTCGAATCT
  781 TCCACTAGAA CCCTTGAGCG TCAGCGAGCT AGAGAACTTA GGAGCGGAGG GACGGGTTCT
  841 GCTAGTAGAC ATCAACAGAT ATCCCTTCAC GCTCCCTTCA TTGTATGCGT GAGTATGTAT
  901 CGCGGAGCAA CACAGTCTTG TCCATCAAAG GGCATTTCTC CACGAATCTC GCACTAGCTT
  961 GGAAATCATA ATCTCGAGTC ACTTTGATTT CGTAACGCTG AATCCCTTTC TAAATGGACT
 1021 CTATGTTGTA AAGGCGAGAC GCTATAACGA TGTCTATCGA CCCGGGACGC ATGACGCATA
1081 CACATAGCCT AGTGAGTTGT GTGACTTCTT GATGACTCGC GTTCCACCTT CTTTGG
//
Primer: M13fwd
Sequence file: RDB14303_A6Hud.seq check
>D03684A1_A6Hu_1_M13fwd_B01_04_ABI24.ab1
    1 TAAATATACG GCGATTGATT TAGCGAGCCG CGAATTCGCC CTTGGAGGAC TTGAGGCTCT
   61 GTGACTATAC TCTTGAGATG AGGAAGACTA TGGTCTTCTT ACAGAAACTC AGCCGTGGCA
  121 TGATAGCGGA GTAAAAGCCT TCATGTTGGT TTCCGACCAT GTTGTTATCC ATAGTCACAA
  181 CAAGCGTCGG AATAACCATG GGATGCTCCT GGACGCGCTG AGGGGGTTGA ACCTTCTGAT
  241 CAATCGTGGT AAGCTTAGGA GGATCCGCAC TGTTTAATCA TCTTCGCAGG GGCCAGTCTC
  301 CGAGTTGGAA GGGCCCAAGC AGCGGTGATC GCTGAATGCA AGAAGGCAAT CAAGAACAAG
  361 GATGCGGCTA CCCTAGTTGG TGAGTCAAGT ATGACTGCCG TCGGCCATTC CGGGTCTGCA
  421 GGTGTGATCT CGCAGGGGGG CAATCTTGGT CCATCAGCAT GATGTTGATG TGCTTATCTT
  481 TCATCATCAT TATCATTGAT TATGTGTGAT AGTCTCAATC ATTAGAGTGC ACTTCTTTGA
  541 GGGTACTCGA GCCGACCGAG TACACACCTC TTTTCCGTCA AAAAATTACA ATGGATTGCG
  601 CCTGCTAACT ATGACGGAGA GAAGACTCAA CTATAAACGT CTGTGGATAT CTACATTCTG
  661 AGGACCAGTG GGCATCCGGG CAGGGTCAGT CGGAGGTATG TTAGGCAGGT CAGCTCCTGT
  721 AAATCAATGA GGATTTGTGT ATCGGCTGCT GCTATCCAGC GCAGCTCAGT ACGAAATTAC
  781 GCGAGATGAA ACAAGTTTGG TTCATCGAGC TGGGTGAATT GAGTATTGGT GTTTTCTGAA
  841 TTGGGTAGTA CAGTTGTTAT ATACATTTGC CACTGCACTC TCCCAACGCA ACACGAACCA
  901 TGGAGCCGGA AGCTCTCGTG ATCGTCGACG GTTACTTGTA GATCCGCTAA CAATGCCAAC
  961 GAGTCACAGA AGAAGAAGAG CCATTCATAA CCAGTACATC CCTCAACGAC TTTGCGATGT
 1021 GACATCGCTT AACCTGCGAG TCACAAAACC CGTTAGCTTC ATTAGGTTTG GTTCATAGAC
1081 CTGCTAGTAC GATTCGTAAA GAGTAGAAGA GATAATCAAT GGCCTACA
//
Primer: Red-N_R
Sequence file: RDB14303_A6Hue.seq check
>D03684A1_A6Hu_1_Red-N_R_D01_10_ABI24.ab1
    1 GAATTCATTT GCACTTGAGC GCATGAACTC CTTGATGATG GCCATGTTAT CCTCCTCGCC
   61 CTTGCTCACG GATCCGAGCT CCATATGCAC TTTGTGATTC ATGATTGCGT TGAACAGCGG
  121 GATAGAGATT CAAGAGTGAC GCGAGTTCAT CAAGAGTTCC ACACAACTCA CTTAGCTATG
  181 TGTATGCGTT CAATGCGTCC GGTCGATAGA CATCGTTATA GCGTCTCGCC TTACAACATA
  241 GAGTCCATTT AGAAGGATTC AGCGTTACGA AATCAAGTGA CTCGAGATTA TGATTTCCAA
  301 GCTAGTGCGA GATTCGTGGA GAAATGCCCT TTGATGGACA AGACTGTGTT GCTCCGCGAT
  361 ACATACTCAC GCATACAATG AAGGGAGCGT GAAGGGATAT CTGTTGATGT CTACTAGCAG
  421 AACCCGTCCC TCCGCTCCTA AGTTCTCTAG CTCGCTGACG CTCAAGGGTT CTAGTGGAAG
  481 ATTCGACTCG AGGGATGCAC ATCGTGGAGC ACTTGGCTCC GACGAGACGA ATCCAACATA
  541 AAATCTATGG ATTTGAGACA CGTTCCTCAT GTGTTGCGTC CCACTAGCCC GGGCCCTATT
  601 CTTGCTCCGG TTAGTTCACT ACAAGCCGGA GCAAAGCGAT GGGTTCGGCA AGTGCAAAGC
  661 ACCTTGCTCA CCGCAGTCCC ACCCACTTGT CGAAGTCGAC AAGCCCGCCC TTGAGCCATG
  721 AAATGATCAG TGATACCGTG AGGTCCCGAT GGGGAATGGA GCCATGATCT CTGCCATCGG
  781 CAGATCCACA ATCATCCAAC TCACCAAGGG ACCTACCTCA CCCAACAAAG GGATCCGGAT
  841 CATCCATGGC ATGATGGGTG CACAGTAACA TACTACCGCT AAGATGAGGG ATTCCTGAAT
  901 CATCTAGATC TGCTTCTACT TTCTGCTAAC ACAAGAGAGC TGTTGAGCTG AATCAACGCA
  961 CCTTTCTACT GCTACCGGTC AGCTCTGCCA TCACATCAAT TTACAAACGG GCGCAAACCA
 1021 TGGCTGGGGA AGTACGAACC CTGCCACTTT TGGTCTCATC GACATTCAGT TGCTCACGTT
 1081 CACCACTGGG CTGTGGGAGT TCATTCGATC GGATGGACTC GGACCCGCAT TCCGGAAACT
1141 TGCA
//
Primer: Sh-ble C
Sequence file: RDB14303_A6Huf.seq check
>D03684A1_A6Hu_1_Sh-ble C_E01_13_ABI24.ab1
    1 GGGAAGTATA GCACACATCT AGTTGAGATC TACATCAATG TTCGAAATCA ACCATGCTTA
   61 TAGCCATTGA TATCTCTCTA CTCTTTTCCG ACCCTTATTA CAAGGTCTAT GACCAAACTA
  121 TTGAGCCTAC GGTTTGTGAC TCGCCAGGTT AAGCGATGTC ACATCGCAAG TCGTTGAGGA
  181 TGTACTGGTT ATGAATGGCT CTTCTTTCTT TCTGTGACTC GTTGGCATTG TTAGCGGATC
  241 TACAAGTAAC CGTCGACGAT CACGAGAGCT TCCGGCTCCA TGGTTCGTGT TGCGTTGGGA
  301 GAGTGCAGTG GCAAATGTAT ATAACAACTG TACTACCCAA TTCAGAAAAC ACCAATACTC
  361 AATTCACCCA GCTCGATGAA CCAAACTTGT TTCATCTCGC GTAATTTCGT ACTGAGCTGC
  421 GCTGGATAGC AGCAGCCGAT ACACAAATCC TCATTGATTT ACAGGAGCTG ACCTGCCTAA
  481 CATACCTCCG ACTGACCCTG CCCGGATGCC CACTGGTCCT CAGAATGTAG ATATCCACAG
  541 ACGTTTATAG TTGAGTCTTC TCTCCGTCAT AGTTAGCAGG CGCAATCCAT TGTAATTTTT
  601 TGACGGAAAA GAGGTGTGTA CTCGGTCGGC TCGAGTACCC TCAAAGAAGT GCACTCTAAT
  661 GATTGAGACT ATCACACATA ATCAATGATA ATGATGATGA AAGATAAGCA CATCAACATC
  721 ATGCTGATGG ACCAAGATTG CCCCCCTGCG AGATCACACC TGCAGACCCG GAATGGCCGA
  781 CGGCAGTCAT ACTTGACTCA CCAACTAGGG TAGCCGCATC CTTGTTCTTG ATTGCCTTCT
  841 TGCATTCAGC GATCACCGCT GCTTGGGCCC TTCCAACTCG GAGACTGGCC CCTGCGAAGA
  901 TGATTAAACA GTGCGGATCC TCCTAAGCTT ACCACGATTG ATCAGAATGT TCAACCCCCC
  961 TCAGCGCGTC CAGGAGCATC CCATGGGTTA TTCCCGACGC TTGTTGTGAC TATGGGATAA
 1021 CACATGGGTC GGAAACGACA TGACGCTTTT TACTCGGCCT ATCATGGCAA CGCTGAGTTC
1081 TGTAGAGACA TTAGTCATGC TCATCTCGTG AGTTAGTCAC AGAGTCATCC AAGT
//
Primer: Neo-M_R
Sequence file: RDB14303_A6Hug.seq check
>D03684A1_A6Hu_1_Neo-M_R_F01_16_ABI24.ab1
    1 GATGCATAGG CAGGCCAGTC CTTCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG
   61 AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC
  121 ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC
  181 GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC
  241 CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA
  301 TCCTGTCTCT TGATCAGAGC TTGATCCCCT GCGCCATCAG ATCCTTGGCG GCAAGAAAGC
  361 CATCCAGTTT ACTTTGCAGG GCTTCCCAAC CTTACCAGAG GGCGCCCCAG CTGGCAATTC
  421 CGGTTCGCTT GCTGTCCATA AAACCGCCCA GTCTAGCTAT CGCCATGTAA GCCCACTGCA
  481 AGCTACCTGC TTTCTCTTTG CGCTTGCGTT TTCCCTTGTC CAGATAGCCC AGTAGCTGAC
  541 ATTCATCCGG GGTCAGCACC GTTTCTGCGG ACTGGCTTTC TACGTGAAAA GGATCTAGGT
  601 GAAGATCCTT TTTGATAATC TCATGCCTGA CATTTATATT CCCCAGAACA TCAGGTTAAT
  661 GGCGTTTTTG ATGTCATTTT CGCGGTGGCT GAGATCAGCC ACTTCTTCCC CGATAACGGA
  721 GACCGGCACA CTGGCCATAT CGGTGGTCAT CATGCGCCAG CTTTCATCCC CGATATGCAC
  781 CACCGGGTAA AGTTCACGGG AGACTTTATC TGACAGCAGA CGTGCACTGG CCAGGGGGAT
  841 CACCATCCGT CGCCCCGGCG TGTCAATAAT ATCACTCTGT ACATCCACAA ACAGACGATA
  901 ACGGCTCTCT CTTTTATAGG TGTAAACCTT AAACTGCCGT ACGTATAGGC TGCGCAACTG
  961 TTGGGAAAGG GCGATCGGGT GCGGGCCCTC TTTCGCTATT ACGCCCAGCT GGGCGAAAGG
 1021 GGGATGTGCT GCAGGCGATT AGTTGGGTAA CGCCAGGGTT TTTCCCAGTC ACGAACGGTT
1081 GTAAACGACG GACAGTGATT GGTATTACGA ACTTCACTAA TACGGGCGAA T
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Sakamoto, H., Drug selection using bleomycin for transfection of the oyster-infecting parasite Perkinsus marinus. Parasitol. Int. 65 (5 Pt B): 563-566 (2016). PMID 27094226. [link to RRC of NBRP]
reference Fernandez-Robledo, J.A., Transfection of the protozoan parasite Perkinsus marinus. Mol. Biochem. Parasitol. 157 (1): 44-53 (2008). PMID 17996961.