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pGEX4T3-mouse MICAL-L2-C - active Rab8/10 trapper (#RDB14285)

Bacteria expression vector of C-terminal fragment of mouse MICAL-L2.

Clone info. A C-terminal fragment of mouse MICAL-L2 is introduced into pGEX4T3 which is a GST fusion protein expression vector. GST-MICAL-L2-C works as a trapper of the GTP binding-active forms of Rab8/10.
Comment Expression was confirmed by the depositor with pull down assay. Known differences compared with NM_ 174850.3: C to T (Ile synonym) at 2696nt and T to A (Gly synonym) at 2765nt. Note: This clone has lacI instead of lacIq promoter.
Vector backbone pGEX-4T-3 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name mouse MICALL2 cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14285 pGEX4T3-mouse MICAL-L2-C - active Rab8/10 trapper DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEX4T3-mouse MICAL-L2-C - active Rab8/10 trapper was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14285).

Reference section:

Homma, Y., Fukuda, M., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14285_A6H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-RSEQ ()
Sequence file: RDB14285_A6H4a.seq check
>14285_14285_A9EeF_pGEX-RSEQ_A06_01_ABI08.ab1
    1 TCCAATCCCG GAAAAGCGCG AGGCAGATCG TCAGTCAGTC ACGATGCGGC CGCGGGAATT
   61 CGATTTCAGG CCTGCCCGCT TTTGCTCTTC GAGGACCAGA TCTTGGACAA GAAGGACTTG
  121 GACTTCTTTC TCTGGAGGCC CAGGTTCTGG ATCATATTCT CCAGCATTTG GTCCTCTTCC
  181 TGTTCCCGGA GCCGATCCTC ATCCAGGAAG TCAACGATGT CACTTCGGTC ATTCACGGTG
  241 TTCACGTATT GGCTCAGCAG CTCCTGCTCC CGCTGGCGGT CCTGGGGGGA CTTCAGACCC
  301 TCTGGCTTGT CCATCAGCCT GCGCAGCTCA CCCTGGAGGT CCAGCTGCTG CTCCTCCAGG
  361 CGCTGGTCCT TGGACTTGTA CATGAGTTCC GATTCCAGCC TCAACAGTAG CTGCTTCTCA
  421 TGAATAAGCC GGAACCAGTC CACCATGAGG CTATCCTCAG AAGCATCTCC CTCAGCTGCC
  481 CGCAGCCGCT TCTCCAACTC AACTCCCCTG AGCTCCAGGG CGTCCAGTTG GCTCTCAATG
  541 TCCTGCAGCT GCCTCTGCAG CTCCTCCTGG GGAATATAGT CGGGGTGGAG CCTGACTGGG
  601 GAAGTGACAG CTTCACCAGG TGGAGCAAGG CCACTGTCCA GGACTGCAGA CCTCTCTCTT
  661 GACCACCTGG TGGGTGACTT GGATCCACCA CCACCACCGG AAATTCCCGG AGATCCACGC
  721 GGAACCAGAT CCGATTTTGG AGGATGGTCG CCACCACCAA ACGTGGCTTG CCAGCCCTGC
  781 AAAGGCCATG CTATATACTT GCTGGATTTC AAGTACTTAT CAATTTGTGG GATAGCTTCA
  841 ATACGTTTTT TAAAACAAAC TAATTTTGGG AACGCATCCA GGCACATTGG GTCCATGTAT
  901 AAAACAACAT CAAGAGCGTC ATACAACATG AAGTCAGGAT GGGTTACATG ATCACCATTT
  961 AAATATGTTT TATGACATAA ACGATCTTTC GAACATTTTC AGCATTTCAG GTAGCTTTGC
 1021 TAAGAAAATC AACTTTGAGA GTTTCAAAGT CTTTACTATA TGCAATTTCT CGAAACACGT
 1081 ATCTAATAAT CCAAAACCCG CTCCCTCAGG CAATGAATCT TCTGCACGCC CTCTTTTGGA
1141 CAAACCTCAC CTCCACAAAC AGTATGTGTG
//
Primer: PGEX-ptac_up-F ()
Sequence file: RDB14285_A6H4b.seq check
>14285_14285_A9EeF_pGEX-ptac_up_F_A12_01_ABI08.ab1
    1 TGCCCACCAT TCATAAACGG GTTCCTGGCA AATATTCTGA AATGAGCTGT TGACAATTAA
   61 TCATCGGCTC GTATAATGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGT
  121 ATTCATGTCC CCTATACTAG GTTATTGGAA AATTAAGGGC CTTGTGCAAC CCACTCGACT
  181 TCTTTTGGAA TATCTTGAAG AAAAATATGA AGAGCATTTG TATGAGCGCG ATGAAGGTGA
  241 TAAATGGCGA AACAAAAAGT TTGAATTGGG TTTGGAGTTT CCCAATCTTC CTTATTATAT
  301 TGATGGTGAT GTTAAATTAA CACAGTCTAT GGCCATCATA CGTTATATAG CTGACAAGCA
  361 CAACATGTTG GGTGGTTGTC CAAAAGAGCG TGCAGAGATT TCAATGCTTG AAGGAGCGGT
  421 TTTGGATATT AGATACGGTG TTTCGAGAAT TGCATATAGT AAAGACTTTG AAACTCTCAA
  481 AGTTGATTTT CTTAGCAAGC TACCTGAAAT GCTGAAAATG TTCGAAGATC GTTTATGTCA
  541 TAAAACATAT TTAAATGGTG ATCATGTAAC CCATCCTGAC TTCATGTTGT ATGACGCTCT
  601 TGATGTTGTT TTATACATGG ACCCAATGTG CCTGGATGCG TTCCCAAAAT TAGTTTGTTT
  661 TAAAAAACGT ATTGAAGCTA TCCCACAAAT TGATAAGTAC TTGAAATCCA GCAAGTATAT
  721 AGCATGGCCT TTGCAGGGCT GGCAAGCCAC GTTTGGTGGT GGCGACCATC CTCCAAAATC
  781 GGATCTGGTT CCGCGTGGAT CTCCGGGAAT TTCCGGTGGT GGTGGTGGAT CCAAGTCACC
  841 CACCAGGTGG TCAAAGAGAG AGGTCTGCAG TTCCTGGACA GTGGCCTTGC TCCACCTGGT
  901 GAAGCTGTCA CTTTCCCCAG TCAGGCTCCA CCCCGACTAT ATTCCCCAGG AAGGAGCTGC
  961 AGAGGCAGCT TGCAAGGACA ATTGAGAAGC CAACTGGACG CCCTGGAAGC TTCAGGGAAG
 1021 TTGAGTTGGG AAGAAGCCGG CTTGCTGTAG CTGAAGGAGA TGGCTTTCCT GAGGATAAGC
 1081 TCATGGGTTG GAAACTGGTT CCGGCTAATC ATTGAAAAGG CAGGCTACTG TGGAGGGCCT
1141 GGAATTCTGG GACAACT
//
Primer: SV40pA3'element Reverse ()
Sequence file: RDB14285_A6H4c.seq check
>D03692B6_A6H4_1_SV40pA3'element_Reverse_D07_10_ABI24.ab1
    1 AATCGCCAGG GGGGGCGGAC CTATGGAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT
   61 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA
  121 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG
  181 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA
  241 TCTGTGCGGT ATTTCACACC GCATAAATTC CGACACCATC GAATGGCGCA AAACCTTTCG
  301 CGGTATGGCA TGATAGCGCC CGGAAGAGAG TCAATTCAGG GTGGTGAATG TGAAACCAGT
  361 AACGTTATAC GATGTCGCAG AGTATGCCGG TGTCTCTTAT CAGACCGTTT CCCGCGTGGT
  421 GAACCAGGCC AGCCACGTTT CTGCGAAAAC GCGGGAAAAA GTGGAAGCGG CGATGGCGGA
  481 GCTGAATTAC ATTCCCAACC GCGTGGCACA ACAACTGGCG GGCAAACAGT CGTTGCTGAT
  541 TGGCGTTGCC ACCTCCAGTC TGGCCCTGCA CGCGCCGTCG CAAATTGTCG CGGCGATTAA
  601 ATCTCGCGCC GATCAACTGG GTGCCAGCGT GGTGGTGTCG ATGGTAGAAC GAAGCGGCGT
  661 CGAAGCCTGT AAAGCGGCGG TGCACAATCT TCTCGCGCAA CGCGTCAGTG GGCTGATCAT
  721 TAACTATCCG CTGGATGACC AGGATGCCAT TGCTGTGGAA GCTGCCTGCA CTAATGTTCC
  781 GGCGTTATTT CTTGATGTCT CTGACCAGAC ACCCATCAAC AGTATTATTT TTCTCCCATG
  841 AAGACGGTAC GCGACTGGGG CGTGGAGCAT CTGGTCGCAT TTGGGGTCAC CAGCAAATCG
  901 CGCTGTTAGC GGGCCCATTA AGTTCTGTCT CGGCGCGTCT GCGTCTGGCT GGCTGGCATA
  961 AATATCTCAC TCGCAATCAA AATTTCAGCC GGATAGCGGA CGGGGAAGGC GACTTGGGAG
 1021 GTTGCCCATT GTTCCGGTTT TTCAAACAAA CCAATGCCAA ATGGCTTGAA ATTGAAGGCA
1081 ATCGATTCCC AACTGCGGAA TGGCTTGGAT TTGCCAACGT AATTTCAAGG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183. [link to RRC of NBRP]