RDB14285 - pGEX4T3-mouse MICAL-L2-C - active Rab8/10 trapper

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pGEX4T3-mouse MICAL-L2-C - active Rab8/10 trapper

Bacteria expression vector of C-terminal fragment of mouse MICAL-L2.

Catalog number	RDB14285
Resource name	pGEX4T3-mouse MICAL-L2-C - active Rab8/10 trapper
Clone info.	A C-terminal fragment of mouse MICAL-L2 is introduced into pGEX4T3 which is a GST fusion protein expression vector. GST-MICAL-L2-C works as a trapper of the GTP binding-active forms of Rab8/10.
Comment	Expression was confirmed by the depositor with pull down assay. Known differences compared with NM_ 174850.3: C to T (Ile synonym) at 2696nt and T to A (Gly synonym) at 2765nt. Note: This clone has lacI instead of lacIq promoter.
Vector backbone	pGEX-4T-3 (Plasmid)
Size of vector backbone	5.0 kb
Selectable markers	Amp^r
Gene/insert name	mouse MICALL2 cDNA
Depositor\|Developer	Fukuda, Mitsunori \|

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mouse MICALL2

External Database
mouse MICALL2

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14285	pGEX4T3-mouse MICAL-L2-C - active Rab8/10 trapper	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original	Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.

Further references such as user reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14285_A6H4p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-RSEQ Sequence file: RDB14285_A6H4a.seq
>14285_14285_A9EeF_pGEX-RSEQ_A06_01_ABI08.ab1 1 TCCAATCCCG GAAAAGCGCG AGGCAGATCG TCAGTCAGTC ACGATGCGGC CGCGGGAATT 61 CGATTTCAGG CCTGCCCGCT TTTGCTCTTC GAGGACCAGA TCTTGGACAA GAAGGACTTG 121 GACTTCTTTC TCTGGAGGCC CAGGTTCTGG ATCATATTCT CCAGCATTTG GTCCTCTTCC 181 TGTTCCCGGA GCCGATCCTC ATCCAGGAAG TCAACGATGT CACTTCGGTC ATTCACGGTG 241 TTCACGTATT GGCTCAGCAG CTCCTGCTCC CGCTGGCGGT CCTGGGGGGA CTTCAGACCC 301 TCTGGCTTGT CCATCAGCCT GCGCAGCTCA CCCTGGAGGT CCAGCTGCTG CTCCTCCAGG 361 CGCTGGTCCT TGGACTTGTA CATGAGTTCC GATTCCAGCC TCAACAGTAG CTGCTTCTCA 421 TGAATAAGCC GGAACCAGTC CACCATGAGG CTATCCTCAG AAGCATCTCC CTCAGCTGCC 481 CGCAGCCGCT TCTCCAACTC AACTCCCCTG AGCTCCAGGG CGTCCAGTTG GCTCTCAATG 541 TCCTGCAGCT GCCTCTGCAG CTCCTCCTGG GGAATATAGT CGGGGTGGAG CCTGACTGGG 601 GAAGTGACAG CTTCACCAGG TGGAGCAAGG CCACTGTCCA GGACTGCAGA CCTCTCTCTT 661 GACCACCTGG TGGGTGACTT GGATCCACCA CCACCACCGG AAATTCCCGG AGATCCACGC 721 GGAACCAGAT CCGATTTTGG AGGATGGTCG CCACCACCAA ACGTGGCTTG CCAGCCCTGC 781 AAAGGCCATG CTATATACTT GCTGGATTTC AAGTACTTAT CAATTTGTGG GATAGCTTCA 841 ATACGTTTTT TAAAACAAAC TAATTTTGGG AACGCATCCA GGCACATTGG GTCCATGTAT 901 AAAACAACAT CAAGAGCGTC ATACAACATG AAGTCAGGAT GGGTTACATG ATCACCATTT 961 AAATATGTTT TATGACATAA ACGATCTTTC GAACATTTTC AGCATTTCAG GTAGCTTTGC 1021 TAAGAAAATC AACTTTGAGA GTTTCAAAGT CTTTACTATA TGCAATTTCT CGAAACACGT 1081 ATCTAATAAT CCAAAACCCG CTCCCTCAGG CAATGAATCT TCTGCACGCC CTCTTTTGGA 1141 CAAACCTCAC CTCCACAAAC AGTATGTGTG //
Primer: PGEX-ptac_up-F Sequence file: RDB14285_A6H4b.seq
>14285_14285_A9EeF_pGEX-ptac_up_F_A12_01_ABI08.ab1 1 TGCCCACCAT TCATAAACGG GTTCCTGGCA AATATTCTGA AATGAGCTGT TGACAATTAA 61 TCATCGGCTC GTATAATGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGT 121 ATTCATGTCC CCTATACTAG GTTATTGGAA AATTAAGGGC CTTGTGCAAC CCACTCGACT 181 TCTTTTGGAA TATCTTGAAG AAAAATATGA AGAGCATTTG TATGAGCGCG ATGAAGGTGA 241 TAAATGGCGA AACAAAAAGT TTGAATTGGG TTTGGAGTTT CCCAATCTTC CTTATTATAT 301 TGATGGTGAT GTTAAATTAA CACAGTCTAT GGCCATCATA CGTTATATAG CTGACAAGCA 361 CAACATGTTG GGTGGTTGTC CAAAAGAGCG TGCAGAGATT TCAATGCTTG AAGGAGCGGT 421 TTTGGATATT AGATACGGTG TTTCGAGAAT TGCATATAGT AAAGACTTTG AAACTCTCAA 481 AGTTGATTTT CTTAGCAAGC TACCTGAAAT GCTGAAAATG TTCGAAGATC GTTTATGTCA 541 TAAAACATAT TTAAATGGTG ATCATGTAAC CCATCCTGAC TTCATGTTGT ATGACGCTCT 601 TGATGTTGTT TTATACATGG ACCCAATGTG CCTGGATGCG TTCCCAAAAT TAGTTTGTTT 661 TAAAAAACGT ATTGAAGCTA TCCCACAAAT TGATAAGTAC TTGAAATCCA GCAAGTATAT 721 AGCATGGCCT TTGCAGGGCT GGCAAGCCAC GTTTGGTGGT GGCGACCATC CTCCAAAATC 781 GGATCTGGTT CCGCGTGGAT CTCCGGGAAT TTCCGGTGGT GGTGGTGGAT CCAAGTCACC 841 CACCAGGTGG TCAAAGAGAG AGGTCTGCAG TTCCTGGACA GTGGCCTTGC TCCACCTGGT 901 GAAGCTGTCA CTTTCCCCAG TCAGGCTCCA CCCCGACTAT ATTCCCCAGG AAGGAGCTGC 961 AGAGGCAGCT TGCAAGGACA ATTGAGAAGC CAACTGGACG CCCTGGAAGC TTCAGGGAAG 1021 TTGAGTTGGG AAGAAGCCGG CTTGCTGTAG CTGAAGGAGA TGGCTTTCCT GAGGATAAGC 1081 TCATGGGTTG GAAACTGGTT CCGGCTAATC ATTGAAAAGG CAGGCTACTG TGGAGGGCCT 1141 GGAATTCTGG GACAACT //
Primer: SV40pA3'element Reverse Sequence file: RDB14285_A6H4c.seq
>D03692B6_A6H4_1_SV40pA3'element_Reverse_D07_10_ABI24.ab1 1 AATCGCCAGG GGGGGCGGAC CTATGGAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT 61 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA 121 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG 181 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA 241 TCTGTGCGGT ATTTCACACC GCATAAATTC CGACACCATC GAATGGCGCA AAACCTTTCG 301 CGGTATGGCA TGATAGCGCC CGGAAGAGAG TCAATTCAGG GTGGTGAATG TGAAACCAGT 361 AACGTTATAC GATGTCGCAG AGTATGCCGG TGTCTCTTAT CAGACCGTTT CCCGCGTGGT 421 GAACCAGGCC AGCCACGTTT CTGCGAAAAC GCGGGAAAAA GTGGAAGCGG CGATGGCGGA 481 GCTGAATTAC ATTCCCAACC GCGTGGCACA ACAACTGGCG GGCAAACAGT CGTTGCTGAT 541 TGGCGTTGCC ACCTCCAGTC TGGCCCTGCA CGCGCCGTCG CAAATTGTCG CGGCGATTAA 601 ATCTCGCGCC GATCAACTGG GTGCCAGCGT GGTGGTGTCG ATGGTAGAAC GAAGCGGCGT 661 CGAAGCCTGT AAAGCGGCGG TGCACAATCT TCTCGCGCAA CGCGTCAGTG GGCTGATCAT 721 TAACTATCCG CTGGATGACC AGGATGCCAT TGCTGTGGAA GCTGCCTGCA CTAATGTTCC 781 GGCGTTATTT CTTGATGTCT CTGACCAGAC ACCCATCAAC AGTATTATTT TTCTCCCATG 841 AAGACGGTAC GCGACTGGGG CGTGGAGCAT CTGGTCGCAT TTGGGGTCAC CAGCAAATCG 901 CGCTGTTAGC GGGCCCATTA AGTTCTGTCT CGGCGCGTCT GCGTCTGGCT GGCTGGCATA 961 AATATCTCAC TCGCAATCAA AATTTCAGCC GGATAGCGGA CGGGGAAGGC GACTTGGGAG 1021 GTTGCCCATT GTTCCGGTTT TTCAAACAAA CCAATGCCAA ATGGCTTGAA ATTGAAGGCA 1081 ATCGATTCCC AACTGCGGAA TGGCTTGGAT TTGCCAACGT AATTTCAAGG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl