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pGEX4T3-mouse MICAL-L2-C - active Rab8/10 trapper (#RDB14285)

Bacteria expression vector of C-terminal fragment of mouse MICAL-L2.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Homma, Y., Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. A C-terminal fragment of mouse MICAL-L2 is introduced into pGEX4T3 which is a GST fusion protein expression vector. GST-MICAL-L2-C works as a trapper of the GTP binding-active forms of Rab8/10.
Comment Expression was confirmed by the depositor with pull down assay. Known differences compared with NM_ 174850.3: C to T (Ile synonym) at 2696nt and T to A (Gly synonym) at 2765nt. Note: This clone has lacI instead of lacIq promoter.
Vector backbone pGEX-4T-3 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Growth conditions LB+Amp, 37oC
Gene/insert name mouse MICALL2 cDNA
Depositor|Developer Fukuda, Mitsunori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)。

Catalog # Resource name Shipping form Fee
RDB14285 pGEX4T3-mouse MICAL-L2-C - active Rab8/10 trapper DNA solution

How to cite this biological resource

Materials & Methods section:

The pGEX4T3-mouse MICAL-L2-C - active Rab8/10 trapper was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14285).

Reference section:

Homma, Y., Fukuda, M., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183. [PubMed] [Article] [RRC of NBRP]