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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-mouse Rabin8 (#RDB14283)

Expression vector of EGFP-tagged mouse Rabin8.

Clone info. Expression vector of EGFP-tagged mouse Rabin8.
Comment Reference sequence is NM_001003950.2. Expression was confirmed by the depositor.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Rabin8 cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)。

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RDB14283 pEGFP-C1-mouse Rabin8 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-mouse Rabin8 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14283).

Reference section:

Homma, Y., Fukuda, M., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14283_A6H4p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB14283_A6H4a.seq check
>D03692B4_A6H4_1_CMV-Forward_E06_15_ABI24.ab1
    1 ACTTTCAACT TCCGGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
   61 ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA GCGCTACCGG TCGCCACCAT
  121 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG
  181 CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG
  241 CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT
  301 CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA
  361 GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT
  421 CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT
  481 GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA
  541 GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG
  601 CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA
  661 CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA
  721 CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT
  781 GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTCCGG
  841 ACTCAGATCC ATGGCTAACG ACCCCTTGGA AGGCTTCCAT GAAGTGAACC TTGCTTCGCC
  901 TACTTCTCCA GACCTTCTTG GTGTGTGTGA CCCGGGGAAA CCCAAGAGCA GACTACCTCC
  961 CCGAGCGTCA TCTACCGGCC ACACCCCCTC CACGTTATGC GCTGCCCCCT CTCCCAGGCC
 1021 CAATGCCTTT AGATCTTTCT GATCTTCCCT ACACCAACCT GTGTTATTCC GTCCCCAGGC
1081 CATTTCCACT GGTGCAGAGT ATCTAAATTA TTCAAGC
//
Primer: EGFP-C
Sequence file: RDB14283_A6H4b.seq check
>D03692B4_A6H4_1_EGFP-C_F06_18_ABI24.ab1
    1 TGGGGGGGGT GGTCTCGGGG ATGGACGAGC TGTGGAGTCC GGACTCAGAT CCATGGCGTA
   61 ACGAGCCCCT TGGAAGGCTT CCATGAAGTG AACCTTGCTT CGCCTACTTC TCCAGACCTT
  121 CTTGGTGTGT GTGACCCGGG AACCCAAGAG CAGACTACCT CCCCGAGCGT CATCTACCGG
  181 CCACACCCCT CCACGTTATG CGCTGCCCCT CTCCAGGCCA ATGCCTTAGA TCTTTCTGAT
  241 CTTCCTACAC AACCTGTGTA TTCGTCCCCC AGGCATTTCA ACTGTGCAGA AGTATCTAAT
  301 ATCAGCGCTC ATGCTCCGGA TCCAGCATCA TCGGTACCCT CTGCGGTAGC ATCAGGGCTC
  361 ACGAAGTTAA CCTCCAGGAA GGACAGCTGT AATGCAGAGA GGGAGTTTCT GCAGGGCGCC
  421 ACCATCACCG AGGCTTCTGC TGGCAATGAT GACATCTTTG GGTTGAGCAC GGATAGCCTG
  481 TCGCGTTTAC GAAGCCCATC TGTTCTGGAA GTTAGAGAGA AGGGCTACGA AAGGTTAAAG
  541 GAAGAACTTG CAAAAGCTCA GAGGGAATTG AAGTTAAAAG ATGAAGAATG TGAGAGACTT
  601 TCGAAAGTGC GGGATCAACT TGGGCAGGAG TTGGAAGAAC TCACAGCTAG TTTGTTTGAG
  661 GAAGCTCACA AGATGGTGAG GGAAGCGAAC GTCAAGCAAG CCACTGCAGA GAAGCAGCTG
  721 AAGGAAGCCC AGGGGAAAAT TGATGTACTT CAGGCTGAAG TGGCCGCTCT GAAGACACTC
  781 GTACTGTCCA GTTCTCCAAC ATCGCCTACG CAGGAGCCTC TGGCGGCTGC AAAGACCCCC
  841 TTTAAAAGGG GGCACACAAG GAACAAAAGT ACAAGCAGCG CCATGGGTGG GGAGCCACCA
  901 GGACTTGAGT GTGATCCAGC CAATCGTAAA AAGACTGCAA AGAGGCTGAT TTATCGCTGT
  961 ATAATGAGTT CAGATCTTTG GAAAGGATGA GCCCACGATG GGACAGAACG TGTCCTTTCC
 1021 CTGGACAAAA TCTACCCAGG AGGAACATCT TTCCATGTTT TACATTTGCA AAGAGTGGAG
1081 TTGGCATCAG CCTGATTCCT GGGAGGCTGT GAAGGAAACC AACCAACCTC CTA
//
Primer: ME-1290Rv
Sequence file: RDB14283_A6H4c.seq check
>D03692B4_A6H4_1_ME-1290RV_G06_21_ABI24.ab1
    1 ATGCAATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT
   61 AAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCAGTT ATCTAGATCC GGTGGATCCC
  121 GGGCCCGCGG TACCGTCGAC CTGCAGGCGG CCGCGAATTC ACTAGTGATT TCAGAGTTCC
  181 TCTTTGAAAT ATCCCAGCTT TGCCAACGAC ATCTCCTTTC TCAACTGCAT AACTTCCCAA
  241 AACATCTGAT CGACATCCTG CTGTTTCACG AGTCCCTGCT GAATGTATCG AATGTATGTA
  301 AAAAAGTTAC ATACAGAAGT GATCCTGTAT CTGCAGAAAG GAGAGATGTA GTAACAGCAG
  361 CTGGAGTCCC CGAGCCTAAT CCTGTGCCTG CAGGGCTTAC TCTGGCCAGT GAGCGCACAC
  421 TTTTTTGGCC CTCCGCACTC GACAGCGGAA GCCTTCACGA AGCGGATGGG CTGCAACCCC
  481 ACTGGTTCGA TGCTTAGAGT GTTGTTCTCC ACAGCCTCCA GAACAGCTGA AGCCAACTCA
  541 CTCTTTGCAA ATGTTAAACA TGGAAAGATG TCCTCCTGGT AGATTTTGTC CAGGAAAGGA
  601 CACGTTCTGT CCATCGTGGG CTCATCCTTC CAAGATCTGA ACTCATTATA CAGCGATAAA
  661 TCAGCCTCTT TGCAGTCTTT TACGATTGGC TGGATCACAC TCAAGTCCTG GTGGCTCCCA
  721 CCCATGGCGC TGCTTGTACT TTTGTTCCTT GTGTGCCCCC TTTTAAAGGG GGTCTTTGCA
  781 GCCGCCAGAG GCTCCTGCGT AGGCGATGTT GGGAGAACTG GGACAGTACG AGTGTCTTCA
  841 GAGCGGCCAC TTCAGCCTGA AGTACATCAA TTTTCCCCCT GGGCTTCCTT CAGCTGCTCT
  901 CTGCAGTGGC TGCTGACGTC GCTTCCCTCC ACATCTGGTG GAGCTCTCAA CAACCTAGCC
  961 TGGTGGAGTC CTCACTCTGC CCAGGTAGAT TCCCGCACTT CCGAAGTCTC TCACAATCTC
 1021 AATCTTACTC AATCCACTTG GACTTGGCAG TCTACGTCAA GCATCGTAGC TATCCTACTC
1081 AG
//
Primer: SV40pro_F_V2
Sequence file: RDB14283_A6H4d.seq check
>D03692B4_A6H4_1_SV40pro_F_V2_H06_24_ABI24.ab1
    1 TGCGCTTTTT TGGAGCTAGG CTTTTGCAAG ATCGATCAAG AGACAGGATG AGGATCGTTT
   61 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
  121 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
  181 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
  241 CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
  301 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
  361 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
  421 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
  481 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
  541 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC
  601 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA
  661 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG
  721 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC
  781 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT
  841 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA
  901 ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCCTTCT ATGAAAGGTT GGGCTTCGGA
  961 AATCGTTTTT CCCGGGACGC CCGGCTGGGA TGATCCCTCC CAGCGCGGGG ATCTCATGCT
 1021 GGGAGTTCTT CGCCCACCCC TAGGGGGGAG CTACTGAACA CGGACGGAGA CATTACTGAA
1081 GCAACCCGCG CTTATGACCG GTCAATA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183. [link to RRC of NBRP]