Resource data sheet
Prev.

pEGFP-C1-mouse Rabin8

Expression vector of EGFP-tagged mouse Rabin8.

Catalog number RDB14283
Resource name pEGFP-C1-mouse Rabin8
Clone info. Expression vector of EGFP-tagged mouse Rabin8.
Reference sequence is NM_001003950.2. Expression was confirmed by the depositor.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Rabin8 cDNA
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Rab3ip (NCBI Gene 216363) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14283 pEGFP-C1-mouse Rabin8 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Mitsunori Fukuda's Rab panel clones (English text)

References

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14283_A6H4p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB14283_A6H4a.seq
>D03692B4_A6H4_1_CMV-Forward_E06_15_ABI24.ab1
    1 ACTTTCAACT TCCGGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
   61 ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA GCGCTACCGG TCGCCACCAT
  121 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG
  181 CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG
  241 CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT
  301 CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA
  361 GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT
  421 CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT
  481 GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA
  541 GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG
  601 CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA
  661 CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA
  721 CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT
  781 GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTCCGG
  841 ACTCAGATCC ATGGCTAACG ACCCCTTGGA AGGCTTCCAT GAAGTGAACC TTGCTTCGCC
  901 TACTTCTCCA GACCTTCTTG GTGTGTGTGA CCCGGGGAAA CCCAAGAGCA GACTACCTCC
  961 CCGAGCGTCA TCTACCGGCC ACACCCCCTC CACGTTATGC GCTGCCCCCT CTCCCAGGCC
 1021 CAATGCCTTT AGATCTTTCT GATCTTCCCT ACACCAACCT GTGTTATTCC GTCCCCAGGC
1081 CATTTCCACT GGTGCAGAGT ATCTAAATTA TTCAAGC
//
Primer: EGFP-C RDB14283_A6H4b.seq
>D03692B4_A6H4_1_EGFP-C_F06_18_ABI24.ab1
    1 TGGGGGGGGT GGTCTCGGGG ATGGACGAGC TGTGGAGTCC GGACTCAGAT CCATGGCGTA
   61 ACGAGCCCCT TGGAAGGCTT CCATGAAGTG AACCTTGCTT CGCCTACTTC TCCAGACCTT
  121 CTTGGTGTGT GTGACCCGGG AACCCAAGAG CAGACTACCT CCCCGAGCGT CATCTACCGG
  181 CCACACCCCT CCACGTTATG CGCTGCCCCT CTCCAGGCCA ATGCCTTAGA TCTTTCTGAT
  241 CTTCCTACAC AACCTGTGTA TTCGTCCCCC AGGCATTTCA ACTGTGCAGA AGTATCTAAT
  301 ATCAGCGCTC ATGCTCCGGA TCCAGCATCA TCGGTACCCT CTGCGGTAGC ATCAGGGCTC
  361 ACGAAGTTAA CCTCCAGGAA GGACAGCTGT AATGCAGAGA GGGAGTTTCT GCAGGGCGCC
  421 ACCATCACCG AGGCTTCTGC TGGCAATGAT GACATCTTTG GGTTGAGCAC GGATAGCCTG
  481 TCGCGTTTAC GAAGCCCATC TGTTCTGGAA GTTAGAGAGA AGGGCTACGA AAGGTTAAAG
  541 GAAGAACTTG CAAAAGCTCA GAGGGAATTG AAGTTAAAAG ATGAAGAATG TGAGAGACTT
  601 TCGAAAGTGC GGGATCAACT TGGGCAGGAG TTGGAAGAAC TCACAGCTAG TTTGTTTGAG
  661 GAAGCTCACA AGATGGTGAG GGAAGCGAAC GTCAAGCAAG CCACTGCAGA GAAGCAGCTG
  721 AAGGAAGCCC AGGGGAAAAT TGATGTACTT CAGGCTGAAG TGGCCGCTCT GAAGACACTC
  781 GTACTGTCCA GTTCTCCAAC ATCGCCTACG CAGGAGCCTC TGGCGGCTGC AAAGACCCCC
  841 TTTAAAAGGG GGCACACAAG GAACAAAAGT ACAAGCAGCG CCATGGGTGG GGAGCCACCA
  901 GGACTTGAGT GTGATCCAGC CAATCGTAAA AAGACTGCAA AGAGGCTGAT TTATCGCTGT
  961 ATAATGAGTT CAGATCTTTG GAAAGGATGA GCCCACGATG GGACAGAACG TGTCCTTTCC
 1021 CTGGACAAAA TCTACCCAGG AGGAACATCT TTCCATGTTT TACATTTGCA AAGAGTGGAG
1081 TTGGCATCAG CCTGATTCCT GGGAGGCTGT GAAGGAAACC AACCAACCTC CTA
//
Primer: ME-1290Rv RDB14283_A6H4c.seq
>D03692B4_A6H4_1_ME-1290RV_G06_21_ABI24.ab1
    1 ATGCAATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT
   61 AAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCAGTT ATCTAGATCC GGTGGATCCC
  121 GGGCCCGCGG TACCGTCGAC CTGCAGGCGG CCGCGAATTC ACTAGTGATT TCAGAGTTCC
  181 TCTTTGAAAT ATCCCAGCTT TGCCAACGAC ATCTCCTTTC TCAACTGCAT AACTTCCCAA
  241 AACATCTGAT CGACATCCTG CTGTTTCACG AGTCCCTGCT GAATGTATCG AATGTATGTA
  301 AAAAAGTTAC ATACAGAAGT GATCCTGTAT CTGCAGAAAG GAGAGATGTA GTAACAGCAG
  361 CTGGAGTCCC CGAGCCTAAT CCTGTGCCTG CAGGGCTTAC TCTGGCCAGT GAGCGCACAC
  421 TTTTTTGGCC CTCCGCACTC GACAGCGGAA GCCTTCACGA AGCGGATGGG CTGCAACCCC
  481 ACTGGTTCGA TGCTTAGAGT GTTGTTCTCC ACAGCCTCCA GAACAGCTGA AGCCAACTCA
  541 CTCTTTGCAA ATGTTAAACA TGGAAAGATG TCCTCCTGGT AGATTTTGTC CAGGAAAGGA
  601 CACGTTCTGT CCATCGTGGG CTCATCCTTC CAAGATCTGA ACTCATTATA CAGCGATAAA
  661 TCAGCCTCTT TGCAGTCTTT TACGATTGGC TGGATCACAC TCAAGTCCTG GTGGCTCCCA
  721 CCCATGGCGC TGCTTGTACT TTTGTTCCTT GTGTGCCCCC TTTTAAAGGG GGTCTTTGCA
  781 GCCGCCAGAG GCTCCTGCGT AGGCGATGTT GGGAGAACTG GGACAGTACG AGTGTCTTCA
  841 GAGCGGCCAC TTCAGCCTGA AGTACATCAA TTTTCCCCCT GGGCTTCCTT CAGCTGCTCT
  901 CTGCAGTGGC TGCTGACGTC GCTTCCCTCC ACATCTGGTG GAGCTCTCAA CAACCTAGCC
  961 TGGTGGAGTC CTCACTCTGC CCAGGTAGAT TCCCGCACTT CCGAAGTCTC TCACAATCTC
 1021 AATCTTACTC AATCCACTTG GACTTGGCAG TCTACGTCAA GCATCGTAGC TATCCTACTC
1081 AG
//
Primer: SV40pro_F_V2 RDB14283_A6H4d.seq
>D03692B4_A6H4_1_SV40pro_F_V2_H06_24_ABI24.ab1
    1 TGCGCTTTTT TGGAGCTAGG CTTTTGCAAG ATCGATCAAG AGACAGGATG AGGATCGTTT
   61 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
  121 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
  181 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
  241 CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
  301 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
  361 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
  421 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
  481 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
  541 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC
  601 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA
  661 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG
  721 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC
  781 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT
  841 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA
  901 ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCCTTCT ATGAAAGGTT GGGCTTCGGA
  961 AATCGTTTTT CCCGGGACGC CCGGCTGGGA TGATCCCTCC CAGCGCGGGG ATCTCATGCT
 1021 GGGAGTTCTT CGCCCACCCC TAGGGGGGAG CTACTGAACA CGGACGGAGA CATTACTGAA
1081 GCAACCCGCG CTTATGACCG GTCAATA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl