Resource data sheet
Prev.

pEGFP-C1-mouse Rabin8-delGEF-300-305 - GEF-activity/Rab11-binding-deficient

Expression vector of EGFP-tagged mouse Rabin8 mutant.

Catalog number RDB14279
Resource name pEGFP-C1-mouse Rabin8-delGEF-300-305 - GEF-activity/Rab11-binding-deficient
Clone info. Expression vector of EGFP-tagged mouse Rabin8 mutant; deletion of 32 AA in the Sec2 domain (171-202 AA) and located after the Sec2 domain (300-305 AA), see Fig. 1A and 5A for details. This mutant lost the GEF activity and binding with Rab11.
Reference sequence is NM_001003950.2. Expression was confirmed by the depositor with SEM.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Rabin8 cDNA
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Rab3ip (NCBI Gene 216363) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14279 pEGFP-C1-mouse Rabin8-delGEF-300-305 - GEF-activity/Rab11-binding-deficient Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Rab family protein (English text)

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.

2019.07.19

GNP_filter3_RDBDEP_html_190432.pl