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pEGFPC1-mouse Rab10-T23N - constitutive negative

Expression vector of EGFP-tagged mouse Rab10 constitutive negative (CN) mutant.

Catalog number RDB14278
Resource name pEGFPC1-mouse Rab10-T23N - constitutive negative
Clone info. Expression vector of EGFP-tagged mouse Rab10 constitutive negative (CN) mutant; T23N. CN mutant mimics the GDP-bound form.
Reference sequence is NM_016676. Expression was confirmed by the depositor with fluorescent microscopy.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Rab10 cDNA
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Rab10 (NCBI Gene 19325) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al., J. Cell Sci. 125, 5177-5187, 2012); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)
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Schedule A (GFP-A) [Link]
Remarks
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RDB14278 pEGFPC1-mouse Rab10-T23N - constitutive negative DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Mitsunori Fukuda's Rab panel clones (English text)

References

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.
original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci. 125 (Pt 21): 5177-5187 (2012). PMID 22854043.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDb14278_A6H4p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDb14278_A6H4a.seq
>D03692A8_A6H4_2_CMV-Forward_A04_01_ABI24.ab1
    1 AGGAAATAAC TTCCGGCCCC AATTGACGCA AATGGGCGGT AAGGCGTGTA CGGTGGGAGG
   61 TCTATATAAG CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC
  121 CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA
  181 CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA
  241 CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC
  301 CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA
  361 GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT
  421 CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGTGG GCGACACCCT
  481 GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA
  541 CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA
  601 CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC
  661 CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA
  721 CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT
  781 CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTC
  841 CGGACTCAGA TCCATGGCGA AGAAGACGTA CGACCTGCTT TTCAGCTGCT CCTGATCGGG
  901 GGACTCGGGA GTGGGCAAGA ACTGCGTCCC TTTTTTCGTT TTTTCGGACG ATGCCCTTCA
  961 ATACCACCCT TTATTTCCCA CCATAGGGAA TAGACTTTTA AGGATCAAAA CAGTGGGAAA
 1021 CTACAAGGAA AGAAGATCAG CCTACAGGAT ATGGGGACAC AGCAGCCAGG AAGCCGATTC
1081 ACATCAATCA CACCTCCTTA CTACAGGAAG GAGGCCAATT G
//
Primer: ME-1290RV RDb14278_A6H4b.seq
>D03692A8_A6H4_2_ME-1290RV_C04_07_ABI24.ab1
    1 ATGGCCAATT CAATTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA
   61 AGTAAAACCT CTACAAATGT GGTATGGCTG ATTATGATCA GTTATCTAGA TCCGGTGGAT
  121 CCCGGGCCCG CGGTACCGTC GACTGCAGAA TTCACTAGTG ATTTCAGCAG CACTTGCTCT
  181 TCCAGCCCGT CACGCCTCCT CCACTGCTGA TATCTACGTT TTCACTGTTG GGTTCTTTTA
  241 CAGGGGTCTT TCGGAGGATG TCTTCAGCTA ATGTGAGGAA CGCCTTTTCG ATGTTTATAT
  301 TTGCTTTTGC ACTAGTCTCA AAAAACCTAA TACCATGCTC CCTTGCAATC TGTTCTCCTT
  361 TGCCTTTCGG TACAACTCTC TTGTCGTCCA TGTCACACTT GTTCCCTAGT AACATTCTTT
  421 CCACATCTTC ATTGGCATGC TCATCTATGT TTCTAAGCCA CTTGCTGATG TTCTCAAAGC
  481 TTTTACCGTT GGTGATGTCA TACACTAGCA TGATGCCCAT TGCTCCTCTG TAGTAGGAGG
  541 TTGTGATGGT GTGAAATCGC TCCTGGCCTG CTGTGTCCCA TATCTGTAGC TTGATCTTCT
  601 TTCCTTGTAG TTCCACTGTT TTGATCTTAA AGTCTATTCC TATGGTGGAA ATAAAGGTGG
  661 TATTGAAGGC ATCGTCCGAA AAACGAAAAA GGACGCAGTT CTTGCCCACT CCCGAGTCCC
  721 CGATCAGGAG CAGCTTGAAA AGCAGGTCGT ACGTCTTCTT CGCCATGGAT CTGAGTCCGG
  781 ACTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGCGGC GGTCACGAAC TCCAGCAGGA
  841 CCATGTGATC GCGCTTCTCG TTGGGGTCTT TGCTCAGGGC GGACTGGGTG CTCAGGTAGT
  901 GGTTGTCGGG CAGCAGCACG GGGCCGTCGC CGATGGGGGT GTTCTGCTGG GTAGTGGTCG
  961 GCGAGCTGCA CGCTGCCGTC CTCGATGTTT GTGGCGATCT GGAAGTTCAC TTGATGCCGG
 1021 TTCTTCCTGC TGGTCGACAT GGATATAGAC GTGTGGCCTG TTGTAAGTGT ACTCAAGCTG
1081 TGCCCCAGGA TGTTGCGATC CTCCTTGAGC
//
Primer: SV40pro_F_V2 RDb14278_A6H4c.seq
>D03692A8_A6H4_2_SV40pro_F_V2_D04_10_ABI24.ab1
    1 TGCGCCTTTT TTGGAGGCTA GGCTTTTGCA AGATCGATCA AGAGACAGGA TGAGGATCGT
   61 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  121 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  181 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  241 AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  301 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  361 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  421 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  481 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  541 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC
  601 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  661 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
  721 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
  781 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
  841 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC
  901 CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG
  961 AATCGTTTTC CCGGGACGCC CGGCTGGATG ATCCCTCCAG CGCGGGGATC TCATGCTGGG
 1021 AGTCTTCGCC CACCCTACGG GGGAAGGCTA CTGGAAACAC GGAAATGAGA CATACTGAGA
1081 ACCCGCCGCT TATGACGGCT AATTA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.
original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci. 125 (Pt 21): 5177-5187 (2012). PMID 22854043.

2019.03.08

GNP_filter3_RDBDEP_html_190307.pl