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pEGFPC1-mouse Rab10-Q68L - constitutive active (#RDB14277)

Expression vector of EGFP-tagged mouse Rab10 constitutive active (CA) mutant.

Clone info. Expression vector of EGFP-tagged mouse Rab10 constitutive active (CA) mutant; Q68L. CA mutant mimics the GTP-bound form.
Comment Reference sequence is NM_016676. Expression was confirmed by the depositor with fluorescent microscopy.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Rab10 cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al., J. Cell Sci. 125, 5177-5187, 2012); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishida, M. et al., J. Cell Sci. 125, 5177-5187, 2012); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)。

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RDB14277 pEGFPC1-mouse Rab10-Q68L - constitutive active DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFPC1-mouse Rab10-Q68L - constitutive active was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14277).

Reference section:

Homma, Y., Fukuda, M., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183. [link to RRC of NBRP]
Ishida, M., Ohbayashi, N., Maruta, Y., Ebata, Y., Fukuda, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14277_A6H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2
Sequence file: RDB14277_A6H4a.seq check
>D03692A7_A6H4_2_EGFP-C2_A08_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGNTCNCNT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG
   61 GATCACTCTC GGCATGGACG AGCTGTACAA GTCCGGACTC AGATCCATGG CGAAGAAGAC
  121 GTACGACCTG CTTTTCAAGC TGCTCCTGAT CGGGGACTCG GGAGTGGGCA AGACCTGCGT
  181 CCTTTTTCGT TTTTCGGACG ATGCCTTCAA TACCACCTTT ATTTCCACCA TAGGAATAGA
  241 CTTTAAGATC AAAACAGTGG AACTACAAGG AAAGAAGATC AAGCTACAGA TATGGGACAC
  301 AGCAGGCCTC GAGCGATTTC ACACCATCAC AACCTCCTAC TACAGAGGAG CAATGGGCAT
  361 CATGCTAGTG TATGACATCA CCAACGGTAA AAGCTTTGAG AACATCAGCA AGTGGCTTAG
  421 AAACATAGAT GAGCATGCCA ATGAAGATGT GGAAAGAATG TTACTAGGGA ACAAGTGTGA
  481 CATGGACGAC AAGAGAGTTG TACCGAAAGG CAAAGGAGAA CAGATTGCAA GGGAGCATGG
  541 TATTAGGTTT TTTGAGACTA GTGCAAAAGC AAATATAAAC ATCGAAAAGG CGTTCCTCAC
  601 ATTAGCTGAA GACATCCTCC GAAAGACCCC TGTAAAAGAA CCCAACAGTG AAAACGTAGA
  661 TATCAGCAGT GGAGGAGGCG TGACGGGCTG GAAGAGCAAG TGCTGCTGAA ATCACTAGTG
  721 AATTCTGCAG TCGACGGTAC CGCGGGCCCG GGATCCACCG GATCTAGATA ACTGATCATA
  781 ATCAGCCATA CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACNTCCC ACACCTCCCC
  841 CNGAACCTGA AACNTAAAAT GAANGCAATT GTTGTTGNTN NNTNNTTTAN TGCAGCTTAT
  901 NANGGTTANA AATAAAGCNA TAGCATCACA AATTTCNNAA ATAAAGCATT TTTTTTTCNC
  961 NGCATTCTAN NNNTGGNTTN NNNNANCTNN TNNNNGNATC NNNANNNNNN NANNNNNNNN
1021 NN
//
Primer: SV40polyA_R2
Sequence file: RDB14277_A6H4b.seq check
>D03692A7_A6H4_2_SV40polyA-R2_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GTTAACAACN NCAATTGCAT TCNTTTTATG TTTCNGGTTC
   61 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG
  121 ATTATGATCA GTTATCTAGA TCCGGTGGAT CCCGGGCCCG CGGTACCGTC GACTGCAGAA
  181 TTCACTAGTG ATTTCAGCAG CACTTGCTCT TCCAGCCCGT CACGCCTCCT CCACTGCTGA
  241 TATCTACGTT TTCACTGTTG GGTTCTTTTA CAGGGGTCTT TCGGAGGATG TCTTCAGCTA
  301 ATGTGAGGAA CGCCTTTTCG ATGTTTATAT TTGCTTTTGC ACTAGTCTCA AAAAACCTAA
  361 TACCATGCTC CCTTGCAATC TGTTCTCCTT TGCCTTTCGG TACAACTCTC TTGTCGTCCA
  421 TGTCACACTT GTTCCCTAGT AACATTCTTT CCACATCTTC ATTGGCATGC TCATCTATGT
  481 TTCTAAGCCA CTTGCTGATG TTCTCAAAGC TTTTACCGTT GGTGATGTCA TACACTAGCA
  541 TGATGCCCAT TGCTCCTCTG TAGTAGGAGG TTGTGATGGT GTGAAATCGC TCGAGGCCTG
  601 CTGTGTCCCA TATCTGTAGC TTGATCTTCT TTCCTTGTAG TTCCACTGTT TTGATCTTAA
  661 AGTCTATTCC TATGGTGGAA ATAAAGGTGG TATTGAAGGC ATCGTCCGAA AAACGAAAAA
  721 GGACGCANGT CTTGCCCACT CCCGAGTCCC CGATCAGGAN CAGCTTGAAN AGCANGTCGT
  781 ACGTCTTCTT CGCCATGGAT CTGANTCCGG ANTTGTACAG CTCGTCCATG CCGAGAGTGA
  841 TCCCGGCGGC GGTCACGAAC TCCAGCANGA NCATGTGATC GCGCTTCTCG TTGGGGNCTT
  901 TGCTCNNGNC GGANNGGGNG CTCNNNANTG NTTGTCGGGC AGCANCACGG GGCCGTCNCC
  961 NANGGGGGGN GNTCTGCNNG NNANNNNNNN GNNANCTGCA NNCNGCCNNN CNNNNNNNNN
1021 GNNNNNGNNN NNTNNNANNN TNNNNNNTNN NNNNCNNNNN NTNNNNCNTN NNNNNNNNN
//
Primer: CMV_Forward
Sequence file: RDB14277_A6H4c.seq check
>D03692A7_A6H4_2_CMV-Forward_E03_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTTG NNGCNAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTACCGGTCG CCACCATGGT
  121 GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA
  181 CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA
  241 GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT
  301 GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA
  361 CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA
  421 GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA
  481 CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT
  541 GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GANAAGCAGA AGAACGGCAT
  601 CNAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGANGGC AGCGNGCAGC TCGCCGANCA
  661 CTACCAGCAG AACACCCCCA TCGGCGACNG CCCCGTGCTG CTGCCCGANN ANCACTACCN
  721 GANCACCCAN NCCGCCCNNA NCAAAGANCC CNANNANNNN CGCGATCACN TNGNNNNNNC
781 NNGNNNNNNN NNN
//
Primer: SV40pro_F_V2
Sequence file: RDB14277_A6H4d.seq check
>D03692A7_A6H4_2_SV40pro_F_V2_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GCTTTTGNAA GNNCGATCAA GAGACAGGNT GAGGNTCGTT
   61 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
  121 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
  181 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
  241 ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
  301 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
  361 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
  421 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
  481 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
  541 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC
  601 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
  661 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
  721 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GNCGGCGAAT GGGCTGACCG
  781 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAN CGCATCGCCT TCTATCGCCT
  841 TCTTGACGAG TTCTTCTGAN CGGGANTCTN GGGTTCGAAA TGACCGACCA AGCGACGCCC
  901 NACCNGCCAT CACGANATTT CGANNNCNCC NNCGCCTTCN ATGAAANNNT NGGNNTTCNN
961 AATCGTTTTC CNGGNNNNNN GNNNGNNTGN NNCNNCNNCG CNGGGNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183. [link to RRC of NBRP]
original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]