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pEGFPC1-mouse Rab10-Q68L - constitutive active

Expression vector of EGFP-tagged mouse Rab10 constitutive active (CA) mutant.

Catalog number RDB14277
Resource name pEGFPC1-mouse Rab10-Q68L - constitutive active
Clone info. Expression vector of EGFP-tagged mouse Rab10 constitutive active (CA) mutant; Q68L. CA mutant mimics the GTP-bound form.
Reference sequence is NM_016676. Expression was confirmed by the depositor with fluorescent microscopy.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Rab10 cDNA
Depositor|Developer Fukuda, Mitsunori |
 
Other clones in our bank mouse Rab10 (NCBI Gene 19325) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al., J. Cell Sci. 125, 5177-5187, 2012); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)
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RDB14277 pEGFPC1-mouse Rab10-Q68L - constitutive active DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.
original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci. 125 (Pt 21): 5177-5187 (2012). PMID 22854043.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Rab family protein (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14277_A6H4p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB14277_A6H4a.seq check
>D03692A7_A6H4_2_EGFP-C2_A08_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGNTCNCNT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG
   61 GATCACTCTC GGCATGGACG AGCTGTACAA GTCCGGACTC AGATCCATGG CGAAGAAGAC
  121 GTACGACCTG CTTTTCAAGC TGCTCCTGAT CGGGGACTCG GGAGTGGGCA AGACCTGCGT
  181 CCTTTTTCGT TTTTCGGACG ATGCCTTCAA TACCACCTTT ATTTCCACCA TAGGAATAGA
  241 CTTTAAGATC AAAACAGTGG AACTACAAGG AAAGAAGATC AAGCTACAGA TATGGGACAC
  301 AGCAGGCCTC GAGCGATTTC ACACCATCAC AACCTCCTAC TACAGAGGAG CAATGGGCAT
  361 CATGCTAGTG TATGACATCA CCAACGGTAA AAGCTTTGAG AACATCAGCA AGTGGCTTAG
  421 AAACATAGAT GAGCATGCCA ATGAAGATGT GGAAAGAATG TTACTAGGGA ACAAGTGTGA
  481 CATGGACGAC AAGAGAGTTG TACCGAAAGG CAAAGGAGAA CAGATTGCAA GGGAGCATGG
  541 TATTAGGTTT TTTGAGACTA GTGCAAAAGC AAATATAAAC ATCGAAAAGG CGTTCCTCAC
  601 ATTAGCTGAA GACATCCTCC GAAAGACCCC TGTAAAAGAA CCCAACAGTG AAAACGTAGA
  661 TATCAGCAGT GGAGGAGGCG TGACGGGCTG GAAGAGCAAG TGCTGCTGAA ATCACTAGTG
  721 AATTCTGCAG TCGACGGTAC CGCGGGCCCG GGATCCACCG GATCTAGATA ACTGATCATA
  781 ATCAGCCATA CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACNTCCC ACACCTCCCC
  841 CNGAACCTGA AACNTAAAAT GAANGCAATT GTTGTTGNTN NNTNNTTTAN TGCAGCTTAT
  901 NANGGTTANA AATAAAGCNA TAGCATCACA AATTTCNNAA ATAAAGCATT TTTTTTTCNC
  961 NGCATTCTAN NNNTGGNTTN NNNNANCTNN TNNNNGNATC NNNANNNNNN NANNNNNNNN
1021 NN
//
Primer: ME-1290RV
Sequence file: RDB14277_A6H4b.seq check
>D03692A7_A6H4_2_SV40polyA-R2_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GTTAACAACN NCAATTGCAT TCNTTTTATG TTTCNGGTTC
   61 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG
  121 ATTATGATCA GTTATCTAGA TCCGGTGGAT CCCGGGCCCG CGGTACCGTC GACTGCAGAA
  181 TTCACTAGTG ATTTCAGCAG CACTTGCTCT TCCAGCCCGT CACGCCTCCT CCACTGCTGA
  241 TATCTACGTT TTCACTGTTG GGTTCTTTTA CAGGGGTCTT TCGGAGGATG TCTTCAGCTA
  301 ATGTGAGGAA CGCCTTTTCG ATGTTTATAT TTGCTTTTGC ACTAGTCTCA AAAAACCTAA
  361 TACCATGCTC CCTTGCAATC TGTTCTCCTT TGCCTTTCGG TACAACTCTC TTGTCGTCCA
  421 TGTCACACTT GTTCCCTAGT AACATTCTTT CCACATCTTC ATTGGCATGC TCATCTATGT
  481 TTCTAAGCCA CTTGCTGATG TTCTCAAAGC TTTTACCGTT GGTGATGTCA TACACTAGCA
  541 TGATGCCCAT TGCTCCTCTG TAGTAGGAGG TTGTGATGGT GTGAAATCGC TCGAGGCCTG
  601 CTGTGTCCCA TATCTGTAGC TTGATCTTCT TTCCTTGTAG TTCCACTGTT TTGATCTTAA
  661 AGTCTATTCC TATGGTGGAA ATAAAGGTGG TATTGAAGGC ATCGTCCGAA AAACGAAAAA
  721 GGACGCANGT CTTGCCCACT CCCGAGTCCC CGATCAGGAN CAGCTTGAAN AGCANGTCGT
  781 ACGTCTTCTT CGCCATGGAT CTGANTCCGG ANTTGTACAG CTCGTCCATG CCGAGAGTGA
  841 TCCCGGCGGC GGTCACGAAC TCCAGCANGA NCATGTGATC GCGCTTCTCG TTGGGGNCTT
  901 TGCTCNNGNC GGANNGGGNG CTCNNNANTG NTTGTCGGGC AGCANCACGG GGCCGTCNCC
  961 NANGGGGGGN GNTCTGCNNG NNANNNNNNN GNNANCTGCA NNCNGCCNNN CNNNNNNNNN
1021 GNNNNNGNNN NNTNNNANNN TNNNNNNTNN NNNNCNNNNN NTNNNNCNTN NNNNNNNNN
//
Primer: SV40pro_F_V2
Sequence file: RDB14277_A6H4c.seq check
>D03692A7_A6H4_2_CMV-Forward_E03_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTTG NNGCNAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTACCGGTCG CCACCATGGT
  121 GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA
  181 CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA
  241 GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT
  301 GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA
  361 CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA
  421 GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA
  481 CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT
  541 GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GANAAGCAGA AGAACGGCAT
  601 CNAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGANGGC AGCGNGCAGC TCGCCGANCA
  661 CTACCAGCAG AACACCCCCA TCGGCGACNG CCCCGTGCTG CTGCCCGANN ANCACTACCN
  721 GANCACCCAN NCCGCCCNNA NCAAAGANCC CNANNANNNN CGCGATCACN TNGNNNNNNC
781 NNGNNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.
original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci. 125 (Pt 21): 5177-5187 (2012). PMID 22854043.

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl