Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEGFPC1-mouse Rab10-Q68L - constitutive active

Expression vector of EGFP-tagged mouse Rab10 constitutive active (CA) mutant.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al., J. Cell Sci. 125, 5177-5187, 2012); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishida, M. et al., J. Cell Sci. 125, 5177-5187, 2012); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14277_A6H4p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 Sequence file: RDB14277_A6H4a.seq
>D03692A7_A6H4_2_EGFP-C2_A08_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGNTCNCNT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG    61 GATCACTCTC GGCATGGACG AGCTGTACAA GTCCGGACTC AGATCCATGG CGAAGAAGAC   121 GTACGACCTG CTTTTCAAGC TGCTCCTGAT CGGGGACTCG GGAGTGGGCA AGACCTGCGT   181 CCTTTTTCGT TTTTCGGACG ATGCCTTCAA TACCACCTTT ATTTCCACCA TAGGAATAGA   241 CTTTAAGATC AAAACAGTGG AACTACAAGG AAAGAAGATC AAGCTACAGA TATGGGACAC   301 AGCAGGCCTC GAGCGATTTC ACACCATCAC AACCTCCTAC TACAGAGGAG CAATGGGCAT   361 CATGCTAGTG TATGACATCA CCAACGGTAA AAGCTTTGAG AACATCAGCA AGTGGCTTAG   421 AAACATAGAT GAGCATGCCA ATGAAGATGT GGAAAGAATG TTACTAGGGA ACAAGTGTGA   481 CATGGACGAC AAGAGAGTTG TACCGAAAGG CAAAGGAGAA CAGATTGCAA GGGAGCATGG   541 TATTAGGTTT TTTGAGACTA GTGCAAAAGC AAATATAAAC ATCGAAAAGG CGTTCCTCAC   601 ATTAGCTGAA GACATCCTCC GAAAGACCCC TGTAAAAGAA CCCAACAGTG AAAACGTAGA   661 TATCAGCAGT GGAGGAGGCG TGACGGGCTG GAAGAGCAAG TGCTGCTGAA ATCACTAGTG   721 AATTCTGCAG TCGACGGTAC CGCGGGCCCG GGATCCACCG GATCTAGATA ACTGATCATA   781 ATCAGCCATA CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACNTCCC ACACCTCCCC   841 CNGAACCTGA AACNTAAAAT GAANGCAATT GTTGTTGNTN NNTNNTTTAN TGCAGCTTAT   901 NANGGTTANA AATAAAGCNA TAGCATCACA AATTTCNNAA ATAAAGCATT TTTTTTTCNC   961 NGCATTCTAN NNNTGGNTTN NNNNANCTNN TNNNNGNATC NNNANNNNNN NANNNNNNNN 1021 NN //
Primer: SV40polyA_R2 Sequence file: RDB14277_A6H4b.seq
>D03692A7_A6H4_2_SV40polyA-R2_A02_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GTTAACAACN NCAATTGCAT TCNTTTTATG TTTCNGGTTC    61 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG   121 ATTATGATCA GTTATCTAGA TCCGGTGGAT CCCGGGCCCG CGGTACCGTC GACTGCAGAA   181 TTCACTAGTG ATTTCAGCAG CACTTGCTCT TCCAGCCCGT CACGCCTCCT CCACTGCTGA   241 TATCTACGTT TTCACTGTTG GGTTCTTTTA CAGGGGTCTT TCGGAGGATG TCTTCAGCTA   301 ATGTGAGGAA CGCCTTTTCG ATGTTTATAT TTGCTTTTGC ACTAGTCTCA AAAAACCTAA   361 TACCATGCTC CCTTGCAATC TGTTCTCCTT TGCCTTTCGG TACAACTCTC TTGTCGTCCA   421 TGTCACACTT GTTCCCTAGT AACATTCTTT CCACATCTTC ATTGGCATGC TCATCTATGT   481 TTCTAAGCCA CTTGCTGATG TTCTCAAAGC TTTTACCGTT GGTGATGTCA TACACTAGCA   541 TGATGCCCAT TGCTCCTCTG TAGTAGGAGG TTGTGATGGT GTGAAATCGC TCGAGGCCTG   601 CTGTGTCCCA TATCTGTAGC TTGATCTTCT TTCCTTGTAG TTCCACTGTT TTGATCTTAA   661 AGTCTATTCC TATGGTGGAA ATAAAGGTGG TATTGAAGGC ATCGTCCGAA AAACGAAAAA   721 GGACGCANGT CTTGCCCACT CCCGAGTCCC CGATCAGGAN CAGCTTGAAN AGCANGTCGT   781 ACGTCTTCTT CGCCATGGAT CTGANTCCGG ANTTGTACAG CTCGTCCATG CCGAGAGTGA   841 TCCCGGCGGC GGTCACGAAC TCCAGCANGA NCATGTGATC GCGCTTCTCG TTGGGGNCTT   901 TGCTCNNGNC GGANNGGGNG CTCNNNANTG NTTGTCGGGC AGCANCACGG GGCCGTCNCC   961 NANGGGGGGN GNTCTGCNNG NNANNNNNNN GNNANCTGCA NNCNGCCNNN CNNNNNNNNN 1021 GNNNNNGNNN NNTNNNANNN TNNNNNNTNN NNNNCNNNNN NTNNNNCNTN NNNNNNNNN //
Primer: CMV_Forward Sequence file: RDB14277_A6H4c.seq
>D03692A7_A6H4_2_CMV-Forward_E03_15_ABI24.ab1     1 NNNNNNNNNN NNNNNNNTTG NNGCNAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA    61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTACCGGTCG CCACCATGGT   121 GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA   181 CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA   241 GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT   301 GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA   361 CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA   421 GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA   481 CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT   541 GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GANAAGCAGA AGAACGGCAT   601 CNAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGANGGC AGCGNGCAGC TCGCCGANCA   661 CTACCAGCAG AACACCCCCA TCGGCGACNG CCCCGTGCTG CTGCCCGANN ANCACTACCN   721 GANCACCCAN NCCGCCCNNA NCAAAGANCC CNANNANNNN CGCGATCACN TNGNNNNNNC 781 NNGNNNNNNN NNN //
Primer: SV40pro_F_V2 Sequence file: RDB14277_A6H4d.seq
>D03692A7_A6H4_2_SV40pro_F_V2_B02_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GCTTTTGNAA GNNCGATCAA GAGACAGGNT GAGGNTCGTT    61 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT   121 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT   181 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA   241 ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC   301 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG   361 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC   421 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA   481 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA   541 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC   601 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA   661 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA   721 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GNCGGCGAAT GGGCTGACCG   781 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAN CGCATCGCCT TCTATCGCCT   841 TCTTGACGAG TTCTTCTGAN CGGGANTCTN GGGTTCGAAA TGACCGACCA AGCGACGCCC   901 NACCNGCCAT CACGANATTT CGANNNCNCC NNCGCCTTCN ATGAAANNNT NGGNNTTCNN 961 AATCGTTTTC CNGGNNNNNN GNNNGNNTGN NNCNNCNNCG CNGGGNNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl