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pEGFPC1-mouse Rab10-Q68L - constitutive active

Expression vector of EGFP-tagged mouse Rab10 constitutive active (CA) mutant.

Catalog number RDB14277
Resource name pEGFPC1-mouse Rab10-Q68L - constitutive active
Clone info. Expression vector of EGFP-tagged mouse Rab10 constitutive active (CA) mutant; Q68L. CA mutant mimics the GTP-bound form.
Reference sequence is NM_016676. Expression was confirmed by the depositor with fluorescent microscopy.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Rab10 cDNA
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Rab10 (NCBI Gene 19325) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al., J. Cell Sci. 125, 5177-5187, 2012); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)
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Schedule A (GFP-A) [Link]
Remarks
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RDB14277 pEGFPC1-mouse Rab10-Q68L - constitutive active DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Mitsunori Fukuda's Rab panel clones (English text)

References

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.
original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci. 125 (Pt 21): 5177-5187 (2012). PMID 22854043.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14277_A6H4p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB14277_A6H4a.seq
>D03692A7_A6H4_2_CMV-Forward_E03_15_ABI24.ab1
    1 ATGTTCACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTACCGGTCG CCACCATGGT
  121 GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA
  181 CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA
  241 GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT
  301 GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA
  361 CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA
  421 GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA
  481 CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT
  541 GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT
  601 CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA
  661 CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT
  721 GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGGTCCTGC
  781 TGGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGA CGAGCTGTAC AAGTCCGGAC
  841 TCAGATCCAT GGCGAGAAGA CGTACGACCT GCTTTTCAGC TGCTCTGATC GGGACTCAGG
  901 AGTGGCAGGA CTTGCGTTCT TTTCGTTTTC GACGATGGCT TCAATACACG TTTATTCACA
  961 TAGAATGGAC CTAGAATCAA CCTGACTCAG AGAGATCAGC TACGATGGAC TGCAGCGTAG
1021 CGATCAGCCA T
//
Primer: ME-1290RV RDB14277_A6H4b.seq
>D03692A7_A6H4_2_ME-1290RV_G03_21_ABI24.ab1
    1 AGGGCAATTT CATTTTATGT TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA
   61 GTAAAACCTC TACAAATGTG GTATGGCTGA TTATGATCAG TTATCTAGAT CCGGTGGATC
  121 CCGGGCCCGC GGTACCGTCG ACTGCAGAAT TCACTAGTGA TTTCAGCAGC ACTTGCTCTT
  181 CCAGCCCGTC ACGCCTCCTC CACTGCTGAT ATCTACGTTT TCACTGTTGG GTTCTTTTAC
  241 AGGGGTCTTT CGGAGGATGT CTTCAGCTAA TGTGAGGAAC GCCTTTTCGA TGTTTATATT
  301 TGCTTTTGCA CTAGTCTCAA AAAACCTAAT ACCATGCTCC CTTGCAATCT GTTCTCCTTT
  361 GCCTTTCGGT ACAACTCTCT TGTCGTCCAT GTCACACTTG TTCCCTAGTA ACATTCTTTC
  421 CACATCTTCA TTGGCATGCT CATCTATGTT TCTAAGCCAC TTGCTGATGT TCTCAAAGCT
  481 TTTACCGTTG GTGATGTCAT ACACTAGCAT GATGCCCATT GCTCCTCTGT AGTAGGAGGT
  541 TGTGATGGTG TGAAATCGCT CGAGGCCTGC TGTGTCCCAT ATCTGTAGCT TGATCTTCTT
  601 TCCTTGTAGT TCCACTGTTT TGATCTTAAA GTCTATTCCT ATGGTGGAAA TAAAGGTGGT
  661 ATTGAAGGCA TCGTCCGAAA AACGAAAAAG GACGCAGGTC TTGCCCACTC CCGAGTCCCC
  721 GATCAGGAGC AGCTTGAAAA GCAGGTCGTA CGTCTTCTTC GCCATGGATC TGAGTCCGGA
  781 CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG GTCACGAACT CCAGCAGGAC
  841 CAATGTGAAT CGCGCTTCTC GTTGGGGGTC TTTGCTCAGG GCGGGACTGG GTGCTCAGGT
  901 AGTGGGTTGT CGGGCAACAA GCACGGGGGC CGTCGCCGAA TGGGGGTGTT CCTGCCTGGT
  961 AGGTGGGGTC GGGCGGAGCT GCACGGCTGG CCGTCCTCGA ATGTTTGTGG CCGCATCTTG
 1021 GAAGTTTCAA CGTTGGATGC CGATTCTTCT GCCTTTGTCG GGCCAATGGA ATAATAGAAC
 1081 CGTTGTGGCC TGGTTGTAGA TTAGTAACTC CAAGCTGTGG CCTCAAGGAT TGTTGCCAGA
1141 TCCCTCCCCT GTGACAAAGT CTGCA
//
Primer: SV40pro_F_V2 RDB14277_A6H4c.seq
>D03692A7_A6H4_2_SV40pro_F_V2_H03_24_ABI24.ab1
    1 CACGAGAATT TTTGGGGAGG CCTAGGCTTT TGCAAGATCG ATCAAAGAGA CAGGATGAGG
   61 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA
  121 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT
  181 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT
  241 GAATGAACTG CAAGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG
  301 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT
  361 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC
  421 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC
  481 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA
  541 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGAG
  601 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT
  661 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG
  721 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC
  781 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA
  841 TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG
  901 ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGG
  961 CTTCGGAATC GTTTTCCCGG GGACGCCGGC TGGGATGATC CCTCCCAGCG CGGGGGATCT
 1021 CATGCTGGGA GGTTTCTTCG CCCAACCCTA GGGGGAGCTA CTGGAAACAC GAGGAGACCA
1081 TTACAGGAAG GAATCCGGCG GCTATTGACC GGCCAATTA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.
original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci. 125 (Pt 21): 5177-5187 (2012). PMID 22854043.

2019.03.08

GNP_filter3_RDBDEP_html_190307.pl