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pEGFPC1-mouse Rab10-Q68L - constitutive active (#RDB14277)

Expression vector of EGFP-tagged mouse Rab10 constitutive active (CA) mutant.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Homma, Y., Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183. [PubMed] [Article] [RRC of NBRP]
Publication Ishida, M., J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of EGFP-tagged mouse Rab10 constitutive active (CA) mutant; Q68L. CA mutant mimics the GTP-bound form.
Comment Reference sequence is NM_016676. Expression was confirmed by the depositor with fluorescent microscopy.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Growth conditions LB+Kanamycin, 37oC
Gene/insert name mouse Rab10 cDNA
Depositor|Developer Fukuda, Mitsunori |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al., J. Cell Sci. 125, 5177-5187, 2012); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishida, M. et al., J. Cell Sci. 125, 5177-5187, 2012); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)。

Catalog # Resource name Shipping form Fee
RDB14277 pEGFPC1-mouse Rab10-Q68L - constitutive active DNA solution

How to cite this biological resource

Materials & Methods section:

The pEGFPC1-mouse Rab10-Q68L - constitutive active was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14277).

Reference section:

Homma, Y., Fukuda, M., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183. [PubMed] [Article] [RRC of NBRP]
Ishida, M., Ohbayashi, N., Maruta, Y., Ebata, Y., Fukuda, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183. [PubMed] [Article] [RRC of NBRP]
original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [PubMed] [Article] [RRC of NBRP]