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pEGFPC1-mouse Rab8A-T22N - constitutive negative

Expression vector of EGFP-tagged mouse Rab8A constitutive negative (CN) mutant.

Catalog number RDB14276
Resource name pEGFPC1-mouse Rab8A-T22N - constitutive negative
Clone info. Expression vector of EGFP-tagged mouse Rab8A constitutive negative (CN) mutant; T22N. CN mutant mimics the GDP-bound form.
Reference sequence is S53270. Expression was confirmed by the depositor with fluorescent microscopy.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Rab8A cDNA
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Rab8a (NCBI Gene 17274) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al., J. Cell Sci. 125, 5177-5187, 2012); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)
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Remarks
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RDB14276 pEGFPC1-mouse Rab8A-T22N - constitutive negative Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.
original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci. 125 (Pt 21): 5177-5187 (2012). PMID 22854043.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Rab family protein (English text)

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.
original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci. 125 (Pt 21): 5177-5187 (2012). PMID 22854043.

2019.07.19

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