Expression vector of EGFP-tagged mouse Rab8A constitutive negative (CN) mutant.
Clone info. | Expression vector of EGFP-tagged mouse Rab8A constitutive negative (CN) mutant; T22N. CN mutant mimics the GDP-bound form. |
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Comment | Reference sequence is S53270. Expression was confirmed by the depositor with fluorescent microscopy. |
Vector backbone | pEGFP-C1 (Plasmid) |
Size of vector backbone | 4.7 kb |
Selectable markers | Kan^r (E. coli), Neo^r (mammalian cells) |
Gene/insert name | mouse Rab8A cDNA |
Depositor|Developer | Fukuda, Mitsunori | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al., J. Cell Sci. 125, 5177-5187, 2012); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016) |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishida, M. et al., J. Cell Sci. 125, 5177-5187, 2012); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB14276 | pEGFPC1-mouse Rab8A-T22N - constitutive negative | DNA solution |
Materials & Methods section:
The pEGFPC1-mouse Rab8A-T22N - constitutive negative was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14276). |
Reference section:
Homma, Y., Fukuda, M., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183. [link to RRC of NBRP] |
Ishida, M., Ohbayashi, N., Maruta, Y., Ebata, Y., Fukuda, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB14276_A6H4p1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: EGFP-C2 (Pr0402) Region: EGFP, insert 5'(T22N) Sequence file: RDB14276_A6H4a.seq ![]() |
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>D03692A6_A6H4_1_EGFP-C2_A09_03_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNGN TCNCNTGGTN CTGCTGGANT TCGTGACCGC CGCCGGGATC 61 ACTCTCGGCA TGGACGAGCT GTACAAGTCC GGACTCAGAT CCATGGCGAA GACCTACGAT 121 TACCTGTTCA AGCTGCTGCT GATCGGGGAC TCGGGGGTAG GGAAGAACTG TGTCCTGTTC 181 CGCTTCTCCG AGGACGCCTT CAACTCCACA TTCATCTCTA CCATAGGAAT TGACTTTAAA 241 ATTAGGACCA TAGAGCTCGA TGGCAAGAGG ATTAAACTGC AGATATGGGA CACGGCCGGC 301 CAGGAGCGGT TTCGAACAAT CACGACAGCC TACTACAGGG GTGCCATGGG TATCATGCTG 361 GTCTACGACA TTACCAATGA GAAGTCCTTT GACAACATCC GGAATTGGAT TCGGAACATT 421 GAAGAGCATG CCTCTGCAGA CGTGGAGAAG ATGATACTGG GGAATAAGTG TGATGTGAAT 481 GACAAGAGAC AGGTGTCCAA GGAACGGGGA GAAAAGCTGG CACTCGACTA TGGGATCAAG 541 TTCATGGAGA CCAGTGCAAA GGCCAACATC AATGTGGAGA ATGCATTTTT CACTCTTGCC 601 AGGGATATCA AAGCAAAAAT GGACAAAAAA TTGGAAGGGA ACAGCCCGCA NGGGAGCAGC 661 CATGGAGTCN AGATCACAGT GGANCAGCAG AAGANGANCA GCTTCTTCCG GTGCAGTCTC 721 CTGTGAAATC ACTAGTGAAT TCGCGGCCGC CTGCANGTCG ANGGTACCGC GGGCCCGGGN 781 NNCNCCGGNT CTAGATAACT GATCATAATC AGCCNTACCA CATTTGTAGA NGNTTTACTT 841 GCTTTAAAAA ANCNNCNNNN NNNCCCCCNN AANNNNAANN NTAAAATGNA NGNNNNNGNN 901 GNNGNNNNNN TGNTNANNNN AGCNNANNNN NNNTNNNNAN NNAANNNNNA NCNTCNNNAN 961 NTNNNNNANN NANNNNNTTT TTTNNNNNNN NNN // |
Primer: SV40polyA_R2 (Pr0623) Region: insert 3'(T22N) Sequence file: RDB14276_A6H4b.seq ![]() |
>D03692A6_A6H4_1_SV40polyA-R2_B09_06_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNTAACNAC AACNATTGCA TTCNTTTTAT GTTTCNGGTT 61 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT 121 GATTATGATC AGTTATCTAG ATCCGGTGGA TCCCGGGCCC GCGGTACCGT CGACCTGCAG 181 GCGGCCGCGA ATTCACTAGT GATTTCACAG GAGACTGCAC CGGAAGAAGC TGGTCCTCTT 241 CTGCTGCTCC ACTGTGATCT TGACTCCATG GCTGCTCCCC TGCGGGCTGT TCCCTTCCAA 301 TTTTTTGTCC ATTTTTGCTT TGATATCCCT GGCAAGAGTG AAAAATGCAT TCTCCACATT 361 GATGTTGGCC TTTGCACTGG TCTCCATGAA CTTGATCCCA TAGTCGAGTG CCAGCTTTTC 421 TCCCCGTTCC TTGGACACCT GTCTCTTGTC ATTCACATCA CACTTATTCC CCAGTATCAT 481 CTTCTCCACG TCTGCAGAGG CATGCTCTTC AATGTTCCGA ATCCAATTCC GGATGTTGTC 541 AAAGGACTTC TCATTGGTAA TGTCGTAGAC CAGCATGATA CCCATGGCAC CCCTGTAGTA 601 GGCTGTCGTG ATTGTTCGAA ACCGCTCCTG GCCGGCCGTG TCCCATATCT GCAGTTTAAT 661 CCTCTTGCCA TCGAGCTCTA TGGTCCTAAT TTTAAAGTCA ATTCCTATGG TAGAGATGAA 721 TGTGGAGTTG AAGGCGTCCT CGGANAANCG GAACAGGACA CAGTTCTTCC CTACCCCCGA 781 GTCCCCGATC AGCAGCAGCT TGAACAGGTA ATCGTANGTC TTCNCCATGG ATCTGANTNC 841 GGANTTGTAC AGCTCGTCCN TGCCNANNNT GATCCCGGNN GCGGNCACGA ACTCCAGCNN 901 NANCNTGTGA TCGCGCTTCN CGTTGGGGNN TTTGCTCNNG GNNGANNGGG NGCTCNNNTA 961 NNGNNNGNNN GGCNNCANCN NNGGGNCNNN NNNNNNGGGG NNNNNNNNNN // |
Primer: CMV_Forward (Pr0016) Region: CMV pro,EGFP Sequence file: RDB14276_A6H4c.seq ![]() |
>D03692A6_A6H4_1_CMV_Forward_G01_19_ABI24.ab1 1 NNNNNNNNNN NNNNNNTTGN NNNNNNGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA 61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGTCGCC ACCATGGTGA 121 GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG 181 TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC 241 TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA 301 CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG 361 ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG 421 ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC 481 GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG 541 AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA 601 AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT 661 ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA 721 NCACCCAGTC CGCCCTGANC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG 781 AGTTCGTGAC CGCCGCCNGG ATCACTCTCG GCATGGACGA NCTGTACAAG TCCGGANTCA 841 GATCCNTGGC GAANANNTAC GANTANCTGT TCNAGCTGCT GCTGATCGGG GANTCNGGGG 901 NANGGAANAA CTGNGNCCNG NNNCGCTTCN NCNANGNNGC NNNCANNNNC NNNTTCNTNN 961 NNNNNNNN // |
Primer: SV40pro_F_V2 (Pr0154) Region: NeoR_KanR Sequence file: RDB14276_A6H4d.seq ![]() |
>D03692A6_A6H4_1_SV40pro_F_V2_A01_01_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNC NNNGCTTTTG NNNNNNCGAT CNAGAGACAG GNTGAGGATC 61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG 121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG 181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA 241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC 301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC 361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA 421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA 481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT 541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT 601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT 661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGANCGCTA 721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA 781 NCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG 841 CCTTCTTGAC GAGTTCTTCT GANCGGGANT CTGGGGTTCG AAATGANCGA NCAANCGNCG 901 CCCNACCNGN CNTCACGANA TTTCGATTCC ACCGCCNNNT TCTATGAAAN GNTGGGCTTC 961 NNNNNCNTTT NNCNGNNNNN CNNNCTNNNN GNNNNNNNNN N // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183. [link to RRC of NBRP] |
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original | Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP] |
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