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pEGFPC1-mouse Rab8A-T22N - constitutive negative

Expression vector of EGFP-tagged mouse Rab8A constitutive negative (CN) mutant.

Catalog number RDB14276
Resource name pEGFPC1-mouse Rab8A-T22N - constitutive negative
Clone info. Expression vector of EGFP-tagged mouse Rab8A constitutive negative (CN) mutant; T22N. CN mutant mimics the GDP-bound form.
Reference sequence is S53270. Expression was confirmed by the depositor with fluorescent microscopy.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Rab8A cDNA
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Rab8a (NCBI Gene 17274) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al., J. Cell Sci. 125, 5177-5187, 2012); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)
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RDB14276 pEGFPC1-mouse Rab8A-T22N - constitutive negative DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.
original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci. 125 (Pt 21): 5177-5187 (2012). PMID 22854043.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Rab family protein (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14276_A6H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2
Region: EGFP, insert 5'(T22N)
Sequence file: RDB14276_A6H4a.seq check
>D03692A6_A6H4_1_EGFP-C2_A09_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGN TCNCNTGGTN CTGCTGGANT TCGTGACCGC CGCCGGGATC
   61 ACTCTCGGCA TGGACGAGCT GTACAAGTCC GGACTCAGAT CCATGGCGAA GACCTACGAT
  121 TACCTGTTCA AGCTGCTGCT GATCGGGGAC TCGGGGGTAG GGAAGAACTG TGTCCTGTTC
  181 CGCTTCTCCG AGGACGCCTT CAACTCCACA TTCATCTCTA CCATAGGAAT TGACTTTAAA
  241 ATTAGGACCA TAGAGCTCGA TGGCAAGAGG ATTAAACTGC AGATATGGGA CACGGCCGGC
  301 CAGGAGCGGT TTCGAACAAT CACGACAGCC TACTACAGGG GTGCCATGGG TATCATGCTG
  361 GTCTACGACA TTACCAATGA GAAGTCCTTT GACAACATCC GGAATTGGAT TCGGAACATT
  421 GAAGAGCATG CCTCTGCAGA CGTGGAGAAG ATGATACTGG GGAATAAGTG TGATGTGAAT
  481 GACAAGAGAC AGGTGTCCAA GGAACGGGGA GAAAAGCTGG CACTCGACTA TGGGATCAAG
  541 TTCATGGAGA CCAGTGCAAA GGCCAACATC AATGTGGAGA ATGCATTTTT CACTCTTGCC
  601 AGGGATATCA AAGCAAAAAT GGACAAAAAA TTGGAAGGGA ACAGCCCGCA NGGGAGCAGC
  661 CATGGAGTCN AGATCACAGT GGANCAGCAG AAGANGANCA GCTTCTTCCG GTGCAGTCTC
  721 CTGTGAAATC ACTAGTGAAT TCGCGGCCGC CTGCANGTCG ANGGTACCGC GGGCCCGGGN
  781 NNCNCCGGNT CTAGATAACT GATCATAATC AGCCNTACCA CATTTGTAGA NGNTTTACTT
  841 GCTTTAAAAA ANCNNCNNNN NNNCCCCCNN AANNNNAANN NTAAAATGNA NGNNNNNGNN
  901 GNNGNNNNNN TGNTNANNNN AGCNNANNNN NNNTNNNNAN NNAANNNNNA NCNTCNNNAN
  961 NTNNNNNANN NANNNNNTTT TTTNNNNNNN NNNNNNNNNN NNNNTNNNNN NNNNNNNNNN
 1021 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNN
//
Primer: SV40polyA_R2
Region: insert 3'(T22N)
Sequence file: RDB14276_A6H4b.seq check
>D03692A6_A6H4_1_SV40polyA-R2_B09_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTAACNAC AACNATTGCA TTCNTTTTAT GTTTCNGGTT
   61 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT
  121 GATTATGATC AGTTATCTAG ATCCGGTGGA TCCCGGGCCC GCGGTACCGT CGACCTGCAG
  181 GCGGCCGCGA ATTCACTAGT GATTTCACAG GAGACTGCAC CGGAAGAAGC TGGTCCTCTT
  241 CTGCTGCTCC ACTGTGATCT TGACTCCATG GCTGCTCCCC TGCGGGCTGT TCCCTTCCAA
  301 TTTTTTGTCC ATTTTTGCTT TGATATCCCT GGCAAGAGTG AAAAATGCAT TCTCCACATT
  361 GATGTTGGCC TTTGCACTGG TCTCCATGAA CTTGATCCCA TAGTCGAGTG CCAGCTTTTC
  421 TCCCCGTTCC TTGGACACCT GTCTCTTGTC ATTCACATCA CACTTATTCC CCAGTATCAT
  481 CTTCTCCACG TCTGCAGAGG CATGCTCTTC AATGTTCCGA ATCCAATTCC GGATGTTGTC
  541 AAAGGACTTC TCATTGGTAA TGTCGTAGAC CAGCATGATA CCCATGGCAC CCCTGTAGTA
  601 GGCTGTCGTG ATTGTTCGAA ACCGCTCCTG GCCGGCCGTG TCCCATATCT GCAGTTTAAT
  661 CCTCTTGCCA TCGAGCTCTA TGGTCCTAAT TTTAAAGTCA ATTCCTATGG TAGAGATGAA
  721 TGTGGAGTTG AAGGCGTCCT CGGANAANCG GAACAGGACA CAGTTCTTCC CTACCCCCGA
  781 GTCCCCGATC AGCAGCAGCT TGAACAGGTA ATCGTANGTC TTCNCCATGG ATCTGANTNC
  841 GGANTTGTAC AGCTCGTCCN TGCCNANNNT GATCCCGGNN GCGGNCACGA ACTCCAGCNN
  901 NANCNTGTGA TCGCGCTTCN CGTTGGGGNN TTTGCTCNNG GNNGANNGGG NGCTCNNNTA
  961 NNGNNNGNNN GGCNNCANCN NNGGGNCNNN NNNNNNGGGG NNNNNNNNNN NNNNNNNNNN
 1021 NNNNNNNNNN NNNNNNNCNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNNNNNNNN NNNNNNNNN
//
Primer: CMV_Forward
Region: CMV pro,EGFP
Sequence file: RDB14276_A6H4c.seq check
>D03692A6_A6H4_1_CMV_Forward_G01_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTTGN NNNNNNGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGTCGCC ACCATGGTGA
  121 GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG
  181 TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC
  241 TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA
  301 CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG
  361 ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG
  421 ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC
  481 GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG
  541 AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA
  601 AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT
  661 ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA
  721 NCACCCAGTC CGCCCTGANC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG
  781 AGTTCGTGAC CGCCGCCNGG ATCACTCTCG GCATGGACGA NCTGTACAAG TCCGGANTCA
  841 GATCCNTGGC GAANANNTAC GANTANCTGT TCNAGCTGCT GCTGATCGGG GANTCNGGGG
  901 NANGGAANAA CTGNGNCCNG NNNCGCTTCN NCNANGNNGC NNNCANNNNC NNNTTCNTNN
  961 NNNNNNNNNN NANNNGNNNT NNAAANNNNN NNNNNNNNNN NNNNNNNNNN NNNGNNNNNA
 1021 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNN
//
Primer: SV40pro_F_V2
Region: NeoR_KanR
Sequence file: RDB14276_A6H4d.seq check
>D03692A6_A6H4_1_SV40pro_F_V2_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNC NNNGCTTTTG NNNNNNCGAT CNAGAGACAG GNTGAGGATC
   61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
  601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGANCGCTA
  721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  781 NCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  841 CCTTCTTGAC GAGTTCTTCT GANCGGGANT CTGGGGTTCG AAATGANCGA NCAANCGNCG
  901 CCCNACCNGN CNTCACGANA TTTCGATTCC ACCGCCNNNT TCTATGAAAN GNTGGGCTTC
  961 NNNNNCNTTT NNCNGNNNNN CNNNCTNNNN GNNNNNNNNN NNNNGGNNNN NNNNGNNNNN
 1021 NNNNNNNNNN NNNCNNNNNN NNNNNNNNNN NANNNNNNNN NNNNNNNNNN NNNNNNNNNN
1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNGNNNN NNNNNNNNNN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.
original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci. 125 (Pt 21): 5177-5187 (2012). PMID 22854043.

2020.01.09

GNP_filter3_RDBDEP_html_191130.pl