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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFPC1-mouse Rab8A-T22N - constitutive negative

Expression vector of EGFP-tagged mouse Rab8A constitutive negative (CN) mutant.

Catalog number RDB14276
Resource name pEGFPC1-mouse Rab8A-T22N - constitutive negative
Clone info. Expression vector of EGFP-tagged mouse Rab8A constitutive negative (CN) mutant; T22N. CN mutant mimics the GDP-bound form.
Comment Reference sequence is S53270. Expression was confirmed by the depositor with fluorescent microscopy.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Rab8A cDNA
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Rab8A

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al., J. Cell Sci. 125, 5177-5187, 2012); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishida, M. et al., J. Cell Sci. 125, 5177-5187, 2012); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)。
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          RDB14276 pEGFPC1-mouse Rab8A-T22N - constitutive negative DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.
          original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci. 125 (Pt 21): 5177-5187 (2012). PMID 22854043.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Rab family protein (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14276_A6H4p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C2 (Pr0402)
          Region: EGFP, insert 5'(T22N)
          Sequence file: RDB14276_A6H4a.seq check
          >D03692A6_A6H4_1_EGFP-C2_A09_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNGN TCNCNTGGTN CTGCTGGANT TCGTGACCGC CGCCGGGATC
             61 ACTCTCGGCA TGGACGAGCT GTACAAGTCC GGACTCAGAT CCATGGCGAA GACCTACGAT
            121 TACCTGTTCA AGCTGCTGCT GATCGGGGAC TCGGGGGTAG GGAAGAACTG TGTCCTGTTC
            181 CGCTTCTCCG AGGACGCCTT CAACTCCACA TTCATCTCTA CCATAGGAAT TGACTTTAAA
            241 ATTAGGACCA TAGAGCTCGA TGGCAAGAGG ATTAAACTGC AGATATGGGA CACGGCCGGC
            301 CAGGAGCGGT TTCGAACAAT CACGACAGCC TACTACAGGG GTGCCATGGG TATCATGCTG
            361 GTCTACGACA TTACCAATGA GAAGTCCTTT GACAACATCC GGAATTGGAT TCGGAACATT
            421 GAAGAGCATG CCTCTGCAGA CGTGGAGAAG ATGATACTGG GGAATAAGTG TGATGTGAAT
            481 GACAAGAGAC AGGTGTCCAA GGAACGGGGA GAAAAGCTGG CACTCGACTA TGGGATCAAG
            541 TTCATGGAGA CCAGTGCAAA GGCCAACATC AATGTGGAGA ATGCATTTTT CACTCTTGCC
            601 AGGGATATCA AAGCAAAAAT GGACAAAAAA TTGGAAGGGA ACAGCCCGCA NGGGAGCAGC
            661 CATGGAGTCN AGATCACAGT GGANCAGCAG AAGANGANCA GCTTCTTCCG GTGCAGTCTC
            721 CTGTGAAATC ACTAGTGAAT TCGCGGCCGC CTGCANGTCG ANGGTACCGC GGGCCCGGGN
            781 NNCNCCGGNT CTAGATAACT GATCATAATC AGCCNTACCA CATTTGTAGA NGNTTTACTT
            841 GCTTTAAAAA ANCNNCNNNN NNNCCCCCNN AANNNNAANN NTAAAATGNA NGNNNNNGNN
            901 GNNGNNNNNN TGNTNANNNN AGCNNANNNN NNNTNNNNAN NNAANNNNNA NCNTCNNNAN
          961 NTNNNNNANN NANNNNNTTT TTTNNNNNNN NNN
          //
          Primer: SV40polyA_R2 (Pr0623)
          Region: insert 3'(T22N)
          Sequence file: RDB14276_A6H4b.seq check
          >D03692A6_A6H4_1_SV40polyA-R2_B09_06_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNTAACNAC AACNATTGCA TTCNTTTTAT GTTTCNGGTT
             61 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT
            121 GATTATGATC AGTTATCTAG ATCCGGTGGA TCCCGGGCCC GCGGTACCGT CGACCTGCAG
            181 GCGGCCGCGA ATTCACTAGT GATTTCACAG GAGACTGCAC CGGAAGAAGC TGGTCCTCTT
            241 CTGCTGCTCC ACTGTGATCT TGACTCCATG GCTGCTCCCC TGCGGGCTGT TCCCTTCCAA
            301 TTTTTTGTCC ATTTTTGCTT TGATATCCCT GGCAAGAGTG AAAAATGCAT TCTCCACATT
            361 GATGTTGGCC TTTGCACTGG TCTCCATGAA CTTGATCCCA TAGTCGAGTG CCAGCTTTTC
            421 TCCCCGTTCC TTGGACACCT GTCTCTTGTC ATTCACATCA CACTTATTCC CCAGTATCAT
            481 CTTCTCCACG TCTGCAGAGG CATGCTCTTC AATGTTCCGA ATCCAATTCC GGATGTTGTC
            541 AAAGGACTTC TCATTGGTAA TGTCGTAGAC CAGCATGATA CCCATGGCAC CCCTGTAGTA
            601 GGCTGTCGTG ATTGTTCGAA ACCGCTCCTG GCCGGCCGTG TCCCATATCT GCAGTTTAAT
            661 CCTCTTGCCA TCGAGCTCTA TGGTCCTAAT TTTAAAGTCA ATTCCTATGG TAGAGATGAA
            721 TGTGGAGTTG AAGGCGTCCT CGGANAANCG GAACAGGACA CAGTTCTTCC CTACCCCCGA
            781 GTCCCCGATC AGCAGCAGCT TGAACAGGTA ATCGTANGTC TTCNCCATGG ATCTGANTNC
            841 GGANTTGTAC AGCTCGTCCN TGCCNANNNT GATCCCGGNN GCGGNCACGA ACTCCAGCNN
            901 NANCNTGTGA TCGCGCTTCN CGTTGGGGNN TTTGCTCNNG GNNGANNGGG NGCTCNNNTA
          961 NNGNNNGNNN GGCNNCANCN NNGGGNCNNN NNNNNNGGGG NNNNNNNNNN
          //
          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,EGFP
          Sequence file: RDB14276_A6H4c.seq check
          >D03692A6_A6H4_1_CMV_Forward_G01_19_ABI24.ab1
              1 NNNNNNNNNN NNNNNNTTGN NNNNNNGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
             61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGTCGCC ACCATGGTGA
            121 GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG
            181 TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC
            241 TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA
            301 CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG
            361 ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG
            421 ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC
            481 GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG
            541 AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA
            601 AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT
            661 ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA
            721 NCACCCAGTC CGCCCTGANC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG
            781 AGTTCGTGAC CGCCGCCNGG ATCACTCTCG GCATGGACGA NCTGTACAAG TCCGGANTCA
            841 GATCCNTGGC GAANANNTAC GANTANCTGT TCNAGCTGCT GCTGATCGGG GANTCNGGGG
            901 NANGGAANAA CTGNGNCCNG NNNCGCTTCN NCNANGNNGC NNNCANNNNC NNNTTCNTNN
          961 NNNNNNNN
          //
          Primer: SV40pro_F_V2 (Pr0154)
          Region: NeoR_KanR
          Sequence file: RDB14276_A6H4d.seq check
          >D03692A6_A6H4_1_SV40pro_F_V2_A01_01_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNC NNNGCTTTTG NNNNNNCGAT CNAGAGACAG GNTGAGGATC
             61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
            121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
            181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
            241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
            301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
            361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
            421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
            481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
            541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
            601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
            661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGANCGCTA
            721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
            781 NCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
            841 CCTTCTTGAC GAGTTCTTCT GANCGGGANT CTGGGGTTCG AAATGANCGA NCAANCGNCG
            901 CCCNACCNGN CNTCACGANA TTTCGATTCC ACCGCCNNNT TCTATGAAAN GNTGGGCTTC
          961 NNNNNCNTTT NNCNGNNNNN CNNNCTNNNN GNNNNNNNNN N
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.
          original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci. 125 (Pt 21): 5177-5187 (2012). PMID 22854043.

          2023.04.10

          GNP_filter3_RDBDEP_html_230405.pl