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pEGFPC1-mouse Rab8A-Q67L - constitutive active (#RDB14275)

Expression vector of EGFP-tagged mouse Rab8A constitutive active (CA) mutant.

Clone info. Expression vector of EGFP-tagged mouse Rab8A constitutive active (CA) mutant; Q67L. CA mutant mimics the GTP-bound form.
Comment Reference sequence is S53270. Expression was confirmed by the depositor with fluorescent microscopy.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Rab8A cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al., J. Cell Sci. 125, 5177-5187, 2012); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishida, M. et al., J. Cell Sci. 125, 5177-5187, 2012); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14275 pEGFPC1-mouse Rab8A-Q67L - constitutive active DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFPC1-mouse Rab8A-Q67L - constitutive active was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14275).

Reference section:

Homma, Y., Fukuda, M., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183. [link to RRC of NBRP]
Ishida, M., Ohbayashi, N., Maruta, Y., Ebata, Y., Fukuda, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14275_A6H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP,insert 5'(Q67L)
Sequence file: RDB14275_A6H4a.seq check
>D03692A5_A6H4_2_EGFP-C2_G08_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNGNTCN CNTGGTCCTG CTGGANTTCG TGACCGCCGC CGGGATCACT
   61 CTCGGCATGG ACGAGCTGTA CAAGTCCGGA CTCAGATCCA TGGCGAAGAC CTACGATTAC
  121 CTGTTCAAGC TGCTGCTGAT CGGGGACTCG GGGGTAGGGA AGACCTGTGT CCTGTTCCGC
  181 TTCTCCGAGG ACGCCTTCAA CTCCACATTC ATCTCTACCA TAGGAATTGA CTTTAAAATT
  241 AGGACCATAG AGCTCGATGG CAAGAGGATT AAACTGCAGA TATGGGACAC AGCCGGCCTG
  301 GAGCGGTTTC GAACAATCAC GACAGCCTAC TACAGGGGTG CCATGGGTAT CATGCTGGTC
  361 TACGACATTA CCAATGAGAA GTCCTTTGAC AACATCCGGA ATTGGATTCG GAACATTGAA
  421 GAGCATGCCT CTGCAGACGT GGAGAAGATG ATACTGGGGA ATAAGTGTGA TGTGAATGAC
  481 AAGAGACAGG TGTCCAAGGA ACGGGGAGAA AAGCTGGCAC TCGACTATGG GATCAAGTTC
  541 ATGGAGACCA GTGCAAAGGC CAACATCAAT GTGGAGAATG CATTTTTCAC TCTTGCCAGG
  601 GATATCAAAG CAAAAATGGA CAAAAAATTG GAAGGGAACA GCCCGCAGGG GAGCAGCCAT
  661 GGAGTCAAGA TCACAGTGGA GCAGCAGAAG ANGANCAGCT TCTTCCGGTG CAGTCTCCTG
  721 TGAAATCACT AGTGAATTCT GCAGTCGACG GTACCGCGGG CCCGGGATCC ACCGGNTCTA
  781 GATAACTGAT CATAATCAGC CNTACCACAT TTGTAGAGGT TTTACTTGCT NTAAAAAACC
  841 TCCCACACCT NCCCCTGAAC CNGAAACATA AAATGAATGN NNTTGNTGNT GTTAACTTGT
  901 TNANTGNAGC TTATNATGGT TANNAATAAA GCNANAGCAT CNNAAATTTC NCAANNNAAG
961 NATTTTTTTT CNNNGNNNTN NNNNNGNNGN NTNNNCNNAN NNNNNNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'(Q67L)
Sequence file: RDB14275_A6H4b.seq check
>D03692A5_A6H4_2_SV40polyA-R2_H08_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNAACN AGTTANCNAN NACNATTGCA TTCNTTTTAT GTTTCNGGTT
   61 CNGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT
  121 GATTATGATC AGTTATCTAG ATCCGGTGGA TCCCGGGCCC GCGGTACCGT CGACTGCAGA
  181 ATTCACTAGT GATTTCACAG GAGACTGCAC CGGAAGAAGC TGGTCCTCTT CTGCTGCTCC
  241 ACTGTGATCT TGACTCCATG GCTGCTCCCC TGCGGGCTGT TCCCTTCCAA TTTTTTGTCC
  301 ATTTTTGCTT TGATATCCCT GGCAAGAGTG AAAAATGCAT TCTCCACATT GATGTTGGCC
  361 TTTGCACTGG TCTCCATGAA CTTGATCCCA TAGTCGAGTG CCAGCTTTTC TCCCCGTTCC
  421 TTGGACACCT GTCTCTTGTC ATTCACATCA CACTTATTCC CCAGTATCAT CTTCTCCACG
  481 TCTGCAGAGG CATGCTCTTC AATGTTCCGA ATCCAATTCC GGATGTTGTC AAAGGACTTC
  541 TCATTGGTAA TGTCGTAGAC CAGCATGATA CCCATGGCAC CCCTGTAGTA GGCTGTCGTG
  601 ATTGTTCGAA ACCGCTCCAG GCCGGCTGTG TCCCATATCT GCAGTTTAAT CCTCTTGCCA
  661 TCGAGCTCTA TGGTCCTAAT TTTAAAGTCA ATTCCTATGG TAGAGATGAA TGTGGAGTTG
  721 AAGGNGTCCT CGGANAANCG GAACAGGACA CAGGTCTTCN CTACCCCCGA GTCNNNGATC
  781 AGCAGCAGCT TGAACAGGTA ATCGTANGTC TTCGNCNTNG NTCTGANTCC GGANTTGTAC
  841 AGCTCGTCCA TGCCGANAGT GATNCCGGCG GCGGNCACNA ACTNCANCAN GANCNTGTGA
  901 TCGCNCTTCT CNNTGGGGNC TTTNCTCNNG NNNNNNTGGG NGCNCNNNNA NNNGNNNGNC
  961 NGNNNNCANC NNNGGNNCNN NNNNCNNNGG GGGNNNNCTG NTNNNNNNNG NNNNGNNANN
1021 NNNNNNNN
//
Primer: CMV_Forward (Pr0016)
Region: .CMV Pro,EGFP
Sequence file: RDB14275_A6H4c.seq check
>D03692A5_A6H4_2_CMV-Forward_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNTGNNN NNNNGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC
  121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA
  181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG
  241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
  301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC
  361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
  421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
  481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
  541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG
  601 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC
  661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC
  721 ACCCAGTCCG CCCTGANCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG
  781 TTCGTGACCG CCGCCGGGNT CACTCTCGGC ATGGNCGANC TGTACAAGTC CNNANTCANA
  841 TCCATGGNGA ANANCTACGA TTACCNNNTC AAGCTGCTGC TNNTCGGGGA NTCNGGGNNA
901 NGNAANANCN NNGNCCNNNT NCGNNTCTNN NNNNANNNNN NNNNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB14275_A6H4d.seq check
>D03692A5_A6H4_2_SV40pro_F_V2_H02_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNCNNNG CTTTTGCAAG NNCGATCNAG AGNNAGGNNG AGGNNCGTTT
   61 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
  121 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
  181 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
  241 CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
  301 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
  361 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
  421 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
  481 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
  541 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC
  601 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA
  661 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA NCGCTATCAN
  721 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC
  781 TTCCTCNTGC TTTACGGTAT CGCCGCTCCN GATTCGCANC GCATCGCCTT CTATCGCCTT
  841 CTTGACGANN NCTTCTGANC GGGANTCTGG GGTTCGAAAT GNNNNANCNA NCGACGCCCA
  901 ACCNNNCNTC NCNANANTTC NNNTNCNNCN NCNNCNTTCN ANNAANNNNN NGGNCNNNNN
961 NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183. [link to RRC of NBRP]
original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]