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pEGFPC1-mouse Rab8A-Q67L - constitutive active

Expression vector of EGFP-tagged mouse Rab8A constitutive active (CA) mutant.

Catalog number RDB14275
Resource name pEGFPC1-mouse Rab8A-Q67L - constitutive active
Clone info. Expression vector of EGFP-tagged mouse Rab8A constitutive active (CA) mutant; Q67L. CA mutant mimics the GTP-bound form.
Reference sequence is S53270. Expression was confirmed by the depositor with fluorescent microscopy.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Rab8A cDNA
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Rab8a (NCBI Gene 17274) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al., J. Cell Sci. 125, 5177-5187, 2012); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)
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RDB14275 pEGFPC1-mouse Rab8A-Q67L - constitutive active DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.
original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Rab family protein (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14275_A6H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2
Region: EGFP,insert 5'(Q67L)
Sequence file: RDB14275_A6H4a.seq check
>D03692A5_A6H4_2_EGFP-C2_G08_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNGNTCN CNTGGTCCTG CTGGANTTCG TGACCGCCGC CGGGATCACT
   61 CTCGGCATGG ACGAGCTGTA CAAGTCCGGA CTCAGATCCA TGGCGAAGAC CTACGATTAC
  121 CTGTTCAAGC TGCTGCTGAT CGGGGACTCG GGGGTAGGGA AGACCTGTGT CCTGTTCCGC
  181 TTCTCCGAGG ACGCCTTCAA CTCCACATTC ATCTCTACCA TAGGAATTGA CTTTAAAATT
  241 AGGACCATAG AGCTCGATGG CAAGAGGATT AAACTGCAGA TATGGGACAC AGCCGGCCTG
  301 GAGCGGTTTC GAACAATCAC GACAGCCTAC TACAGGGGTG CCATGGGTAT CATGCTGGTC
  361 TACGACATTA CCAATGAGAA GTCCTTTGAC AACATCCGGA ATTGGATTCG GAACATTGAA
  421 GAGCATGCCT CTGCAGACGT GGAGAAGATG ATACTGGGGA ATAAGTGTGA TGTGAATGAC
  481 AAGAGACAGG TGTCCAAGGA ACGGGGAGAA AAGCTGGCAC TCGACTATGG GATCAAGTTC
  541 ATGGAGACCA GTGCAAAGGC CAACATCAAT GTGGAGAATG CATTTTTCAC TCTTGCCAGG
  601 GATATCAAAG CAAAAATGGA CAAAAAATTG GAAGGGAACA GCCCGCAGGG GAGCAGCCAT
  661 GGAGTCAAGA TCACAGTGGA GCAGCAGAAG ANGANCAGCT TCTTCCGGTG CAGTCTCCTG
  721 TGAAATCACT AGTGAATTCT GCAGTCGACG GTACCGCGGG CCCGGGATCC ACCGGNTCTA
  781 GATAACTGAT CATAATCAGC CNTACCACAT TTGTAGAGGT TTTACTTGCT NTAAAAAACC
  841 TCCCACACCT NCCCCTGAAC CNGAAACATA AAATGAATGN NNTTGNTGNT GTTAACTTGT
  901 TNANTGNAGC TTATNATGGT TANNAATAAA GCNANAGCAT CNNAAATTTC NCAANNNAAG
  961 NATTTTTTTT CNNNGNNNTN NNNNNGNNGN NTNNNCNNAN NNNNNNNNNN NNNNNNNNNN
 1021 NNNNANNNNN NNNNNNNNNN NTTNNNNAAN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNNNNNNNN NNNNN
//
Primer: SV40polyA_R2
Region: insert 3'(Q67L)
Sequence file: RDB14275_A6H4b.seq check
>D03692A5_A6H4_2_SV40polyA-R2_H08_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNAACN AGTTANCNAN NACNATTGCA TTCNTTTTAT GTTTCNGGTT
   61 CNGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT
  121 GATTATGATC AGTTATCTAG ATCCGGTGGA TCCCGGGCCC GCGGTACCGT CGACTGCAGA
  181 ATTCACTAGT GATTTCACAG GAGACTGCAC CGGAAGAAGC TGGTCCTCTT CTGCTGCTCC
  241 ACTGTGATCT TGACTCCATG GCTGCTCCCC TGCGGGCTGT TCCCTTCCAA TTTTTTGTCC
  301 ATTTTTGCTT TGATATCCCT GGCAAGAGTG AAAAATGCAT TCTCCACATT GATGTTGGCC
  361 TTTGCACTGG TCTCCATGAA CTTGATCCCA TAGTCGAGTG CCAGCTTTTC TCCCCGTTCC
  421 TTGGACACCT GTCTCTTGTC ATTCACATCA CACTTATTCC CCAGTATCAT CTTCTCCACG
  481 TCTGCAGAGG CATGCTCTTC AATGTTCCGA ATCCAATTCC GGATGTTGTC AAAGGACTTC
  541 TCATTGGTAA TGTCGTAGAC CAGCATGATA CCCATGGCAC CCCTGTAGTA GGCTGTCGTG
  601 ATTGTTCGAA ACCGCTCCAG GCCGGCTGTG TCCCATATCT GCAGTTTAAT CCTCTTGCCA
  661 TCGAGCTCTA TGGTCCTAAT TTTAAAGTCA ATTCCTATGG TAGAGATGAA TGTGGAGTTG
  721 AAGGNGTCCT CGGANAANCG GAACAGGACA CAGGTCTTCN CTACCCCCGA GTCNNNGATC
  781 AGCAGCAGCT TGAACAGGTA ATCGTANGTC TTCGNCNTNG NTCTGANTCC GGANTTGTAC
  841 AGCTCGTCCA TGCCGANAGT GATNCCGGCG GCGGNCACNA ACTNCANCAN GANCNTGTGA
  901 TCGCNCTTCT CNNTGGGGNC TTTNCTCNNG NNNNNNTGGG NGCNCNNNNA NNNGNNNGNC
  961 NGNNNNCANC NNNGGNNCNN NNNNCNNNGG GGGNNNNCTG NTNNNNNNNG NNNNGNNANN
 1021 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NN
//
Primer: CMV_Forward
Region: .CMV Pro,EGFP
Sequence file: RDB14275_A6H4c.seq check
>D03692A5_A6H4_2_CMV-Forward_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNTGNNN NNNNGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC
  121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA
  181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG
  241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
  301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC
  361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
  421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
  481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
  541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG
  601 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC
  661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC
  721 ACCCAGTCCG CCCTGANCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG
  781 TTCGTGACCG CCGCCGGGNT CACTCTCGGC ATGGNCGANC TGTACAAGTC CNNANTCANA
  841 TCCATGGNGA ANANCTACGA TTACCNNNTC AAGCTGCTGC TNNTCGGGGA NTCNGGGNNA
  901 NGNAANANCN NNGNCCNNNT NCGNNTCTNN NNNNANNNNN NNNNNNNNNN NNTNNNNNNN
  961 NNNNNNNNNA NNNNNNNTNN AANNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
 1021 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1081 NNNNNNNNNN NNNNNNN
//
Primer: SV40pro_F_V2
Region: NeoR_KanR
Sequence file: RDB14275_A6H4d.seq check
>D03692A5_A6H4_2_SV40pro_F_V2_H02_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNCNNNG CTTTTGCAAG NNCGATCNAG AGNNAGGNNG AGGNNCGTTT
   61 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
  121 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
  181 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
  241 CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
  301 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
  361 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
  421 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
  481 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
  541 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC
  601 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA
  661 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA NCGCTATCAN
  721 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC
  781 TTCCTCNTGC TTTACGGTAT CGCCGCTCCN GATTCGCANC GCATCGCCTT CTATCGCCTT
  841 CTTGACGANN NCTTCTGANC GGGANTCTGG GGTTCGAAAT GNNNNANCNA NCGACGCCCA
  901 ACCNNNCNTC NCNANANTTC NNNTNCNNCN NCNNCNTTCN ANNAANNNNN NGGNCNNNNN
  961 NNNNNNTTNN CNNGNNNNNN NNNNNGNNNN NNNCNNNNNN NNNNNNNNNN NNNNNNNNNN
 1021 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.
original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043.

2020.01.09

GNP_filter3_RDBDEP_html_191130.pl