Resource data sheet
Prev.

pEGFP-C1-mouse Rab10

Expression vector of EGFP-tagged mouse Rab10.

Catalog number RDB14274
Resource name pEGFP-C1-mouse Rab10
Clone info. Expression vector of EGFP-tagged mouse Rab10.
Reference sequence is NM_016676. Expression was confirmed by the depositor with immunoprecipitation.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Rab10 cDNA
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Rab10 (NCBI Gene 19325) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tuboi, T. et al., J. Cell Sci. 119, 2196-2203, 2016); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


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RDB14274 pEGFP-C1-mouse Rab10 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Mitsunori Fukuda's Rab panel clones (English text)

References

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.
original Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14274_A6H4p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB14274_A6H4a.seq
>D03692A4_A6H4_2_CMV-Forward_A02_02_ABI24.ab1
    1 AGGTTCCACA TCCGGCCCCC ATTTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT
   61 CTATATAAGC AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCGCTACC GGTCGCCACC
  121 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC
  181 GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC
  241 GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC
  301 CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG
  361 CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC
  421 TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG
  481 GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC
  541 AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC
  601 GGCATCAAGG TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC
  661 GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC
  721 TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC
  781 CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTCC
  841 GGACTCAGAT CCATGGCGAA GAAGACGTAC GACCTGCTTT TCAAGCTGCT CCTGATCGGG
  901 GGACTCGGGA GTGGGCAAGA CCTGCGTCCT TTTTTCGTTT TTTCGGACGA TGCCCTTCAA
  961 TACCACCCTT TATTTCCACC ATAGGAATAG ACTTTTAAAG ATCAAAACAG TGGGAACTAC
 1021 AAAGGGAAAG AGAATCCAGG CTACCAGATA ATGGGACACA GCCAGTCCAA GAAGCGATTC
1081 ACATCATCAC ATCCTTCCTT ACCTACAGAA AGGGGAAGCC AATTG
//
Primer: ME-1290RV RDB14274_A6H4b.seq
>D03692A4_A6H4_2_ME-1290RV_C02_08_ABI24.ab1
    1 ATTCAATTCA TTTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG
   61 TAAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCAGT TATCTAGATC CGGTGGATCC
  121 CGGGCCCGCG GTACCGTCGA CTGCAGAATT CACTAGTGAT TTCAGCAGCA CTTGCTCTTC
  181 CAGCCCGTCA CGCCTCCTCC ACTGCTGATA TCTACGTTTT CACTGTTGGG TTCTTTTACA
  241 GGGGTCTTTC GGAGGATGTC TTCAGCTAAT GTGAGGAACG CCTTTTCGAT GTTTATATTT
  301 GCTTTTGCAC TAGTCTCAAA AAACCTAATA CCATGCTCCC TTGCAATCTG TTCTCCTTTG
  361 CCTTTCGGTA CAACTCTCTT GTCGTCCATG TCACACTTGT TCCCTAGTAA CATTCTTTCC
  421 ACATCTTCAT TGGCATGCTC ATCTATGTTT CTAAGCCACT TGCTGATGTT CTCAAAGCTT
  481 TTACCGTTGG TGATGTCATA CACTAGCATG ATGCCCATTG CTCCTCTGTA GTAGGAGGTT
  541 GTGATGGTGT GAAATCGCTC CTGGCCTGCT GTGTCCCATA TCTGTAGCTT GATCTTCTTT
  601 CCTTGTAGTT CCACTGTTTT GATCTTAAAG TCTATTCCTA TGGTGGAAAT AAAGGTGGTA
  661 TTGAAGGCAT CGTCCGAAAA ACGAAAAAGG ACGCAGGTCT TGCCCACTCC CGAGTCCCCG
  721 ATCAGGAGCA GCTTGAAAAG CAGGTCGTAC GTCTTCTTCG CCATGGATCT GAGTCCGGAC
  781 TTGTACAGCT CGTCCATGCC GAGAGTGATC CCGGCGGCGG TCACGAACTC CAGCAGGACC
  841 ATGTGATCGC GCTTCTCGTT GGGGTCTTTG CTCAGGGCGG ACTGGGTGCT CAGGTAGTGG
  901 TTGTCGGGCA GCAGCACGGG GCCGTCGCCG ATGGGGGTGT TCTGCTGGGT AGTGGGTCGG
  961 CGAGCTGCAC GCTGCCGTCC TCGATGTTGT GGCGGATCTT GGAAGTTTCA CCTTGATGCC
 1021 GTTCTCTGCT GTCGCCATGA TATAGACGTT GTGCCTGGTT GTAGTGGTAC CTCCAGCTGT
1081 GCCCCAGGAA TGTGCGTTCC TTCCTTGAAA GTCCGA
//
Primer: SV40pro_F_V2 RDB14274_A6H4c.seq
>D03692A4_A6H4_2_SV40pro_F_V2_D02_11_ABI24.ab1
    1 TGCACTTTTG GAGCTAGGCT TTTGCAAGAT CGATCAAGAG ACAGGATGAG GATCGTTTCG
   61 CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT
  121 CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC
  181 AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT
  241 GCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT
  301 GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA
  361 GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT
  421 GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG
  481 CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA
  541 AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA GCATGCCCGA
  601 CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA
  661 TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA
  721 CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT
  781 CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT
  841 TGACGAGTTC TTCTGAGCGG GACTCTGGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA
  901 CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT
  961 CGTTTTCCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGGAGTTC
 1021 TTCGCCCACC CTACGGGGGA AGGCTTACCT GAAACACGGC AAGCGAGACA TTACTGGCAA
1081 GCAGCCCGCC GCTATGACGG CCAATTAGA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.
original Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl