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pEGFP-C1-mouse Rab10 (#RDB14274)

Expression vector of EGFP-tagged mouse Rab10.

Clone info. Expression vector of EGFP-tagged mouse Rab10.
Comment Reference sequence is NM_016676. Expression was confirmed by the depositor with immunoprecipitation.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Rab10 cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tuboi, T. et al., J. Cell Sci. 119, 2196-2203, 2016); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tuboi, T. et al., J. Cell Sci. 119, 2196-2203, 2016); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14274 pEGFP-C1-mouse Rab10 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-mouse Rab10 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14274).

Reference section:

Homma, Y., Fukuda, M., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183. [link to RRC of NBRP]
Tsuboi, T., Fukuda, M., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14274_A6H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3', insert
Sequence file: RDB14274_A6H4a.seq check
>D03692A4_A6H4_2_EGFP-C2_G07_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNTC NCNTGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC
   61 TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCC ATGGCGAAGA AGACGTACGA
  121 CCTGCTTTTC AAGCTGCTCC TGATCGGGGA CTCGGGAGTG GGCAAGACCT GCGTCCTTTT
  181 TCGTTTTTCG GACGATGCCT TCAATACCAC CTTTATTTCC ACCATAGGAA TAGACTTTAA
  241 GATCAAAACA GTGGAACTAC AAGGAAAGAA GATCAAGCTA CAGATATGGG ACACAGCAGG
  301 CCAGGAGCGA TTTCACACCA TCACAACCTC CTACTACAGA GGAGCAATGG GCATCATGCT
  361 AGTGTATGAC ATCACCAACG GTAAAAGCTT TGAGAACATC AGCAAGTGGC TTAGAAACAT
  421 AGATGAGCAT GCCAATGAAG ATGTGGAAAG AATGTTACTA GGGAACAAGT GTGACATGGA
  481 CGACAAGAGA GTTGTACCGA AAGGCAAAGG AGAACAGATT GCAAGGGAGC ATGGTATTAG
  541 GTTTTTTGAG ACTAGTGCAA AAGCAAATAT AAACATCGAA AAGGCGTTCC TCACATTAGC
  601 TGAAGACATC CTCCGAAAGA CCCCTGTAAA AGAACCCAAC AGTGAAAACG TAGATATCAG
  661 CAGTGGAGGA GGCGTGACGG GCTGGAAGAG CAAGTGCTGC TGAAATCACT AGTGAATTCT
  721 GCAGTCGACG GTACCGCGGG CCCGGGATCC ACCGGATCTA GATAACTGAT CATAATCAGC
  781 CATACCACAT TTGTAGAGGT TTTACTTGCT TTNAAAAACC TCCCACACCT CCCCNNGAAC
  841 CTGAAACATA AAATGAATGN NATTGNTGTT GTTNACTTGN TTATNGCAGC TTATAATGGT
  901 TANAAATAAA NNAATANCNT CNCAAATTTC ACNAATAAAG CATTTTTTTT CNNNGNNTNC
961 NANNTNGNNG NNTTGNNNNA NCNCNTCNNN NNNNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert
Sequence file: RDB14274_A6H4b.seq check
>D03692A4_A6H4_2_SV40polyA-R2_H07_22_ABI24.ab1
    1 NNNNNNNNNN CNGNNNNNNN NNNGTTNNNN NNNACAATTG CATTCNTTTT ATGTTTCNGG
   61 TTCNGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
  121 CTGATTATGA TCAGTTATCT AGATCCGGTG GATCCCGGGC CCGCGGTACC GTCGACTGCA
  181 GAATTCACTA GTGATTTCAG CAGCACTTGC TCTTCCAGCC CGTCACGCCT CCTCCACTGC
  241 TGATATCTAC GTTTTCACTG TTGGGTTCTT TTACAGGGGT CTTTCGGAGG ATGTCTTCAG
  301 CTAATGTGAG GAACGCCTTT TCGATGTTTA TATTTGCTTT TGCACTAGTC TCAAAAAACC
  361 TAATACCATG CTCCCTTGCA ATCTGTTCTC CTTTGCCTTT CGGTACAACT CTCTTGTCGT
  421 CCATGTCACA CTTGTTCCCT AGTAACATTC TTTCCACATC TTCATTGGCA TGCTCATCTA
  481 TGTTTCTAAG CCACTTGCTG ATGTTCTCAA AGCTTTTACC GTTGGTGATG TCATACACTA
  541 GCATGATGCC CATTGCTCCT CTGTAGTAGG AGGTTGTGAT GGTGTGAAAT CGCTCCTGGC
  601 CTGCTGTGTC CCATATCTGT AGCTTGATCT TCTTTCCTTG TAGTTCCACT GTTTTGATCT
  661 TAAAGTCTAT TCCTATGGTG GAAATAAAGG TGGTATTGAA GGCATCGTCC GAAAAAACGA
  721 AAAAGGACGC AGGTCTTGCC CACTCCCGAG TCCCCGATCA GGAGCAGCTT GAAAAGCAGG
  781 TCGTACGTCT TCTTCGCCAT GGATCTGANT CCGGACTTGT ACAGCTCGTC CATGCCGAGA
  841 NTGATCCCGG CGGCGGNCAC NAACTNCAGC AGGANNNTGT GATCNCGCTT NTCGNTGGGG
  901 NCTTTGCTCN NGNCNGNNTG GGTGCTCNNN TANNGGNTGT CGGGCANCAN CNCGGGNNNN
  961 TCNNCNNANG GGGGTGTNCT GCNNGNTNNN GNTCNGCNNN NTGNNNNCNN NCGTCNNNNN
1021 NNTNGNNNNN GNANNNNNNN NTNNNNNNNN NN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro, EGFP
Sequence file: RDB14274_A6H4c.seq check
>D03692A4_A6H4_2_CMV-Forward_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCCCN NNNNNNNGCN NNNGGGCGGT AGGCGTGTAC GGTGGGAGGT
   61 CTATATAAGC AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCGCTACC GGTCGCCACC
  121 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC
  181 GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC
  241 GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC
  301 CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG
  361 CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC
  421 TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG
  481 GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC
  541 AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC
  601 GGCATCAAGG TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC
  661 GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC
  721 TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC
  781 CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGANCT GTACAAGTCC
  841 NGACTCAGAT NCATGGCGAA GAAGANGTAC GANCNNCTTT TCAAGCTGCT CCNGATNNGG
  901 GGNNTCNGGA NNNGGNNNNA NCNNCNNNCN TTTTTCNNTT TTTNNGANNA TGNCNTTCNN
961 NNNNNNCNTT TNNTTNCNNN NNNNNNN
//
Primer: SV40_pro_F_V2 (Pro0154)
Region: NeoR_KanR
Sequence file: RDB14274_A6H4d.seq check
>D03692A4_A6H4_2_SV40pro_F_V2_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNCNNNGCT TTTGCAAGNN CGATCNNGAG NNAGGNNGAG GNNCGTTTCG
   61 CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT
  121 CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC
  181 AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT
  241 GCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT
  301 GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA
  361 GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT
  421 GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG
  481 CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA
  541 AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA GCATGCCCGA
  601 CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA
  661 TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGANC GCTATCAGGA
  721 CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT
  781 CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGNCTTCT
  841 TGACGANTTC TTCTGANCGG GNNTCTGGGG NTTCNAAATG ANCGANCNAG CGACGCCCNA
901 CCNNCNNTCA CNANNTTNCN ANNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183. [link to RRC of NBRP]
original Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [link to RRC of NBRP]