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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-mouse Rab8A (#RDB14273)

Expression vector of EGFP-tagged mouse Rab8A.

Clone info. Expression vector of EGFP-tagged mouse Rab8A.
Comment Reference sequence is S53270. Expression was confirmed by the depositor with immunoprecipitation.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Rab8A cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tuboi, T. et al., J. Cell Sci. 119, 2196-2203, 2016); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)
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提供依頼書  [依頼書の記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tuboi, T. et al., J. Cell Sci. 119, 2196-2203, 2016); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14273 pEGFP-C1-mouse Rab8A DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-mouse Rab8A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14273).

Reference section:

Homma, Y., Fukuda, M., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183. [link to RRC of NBRP]
Tsuboi, T., Fukuda, M., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14273_A6H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP, insert 5'
Sequence file: RDB14273_A6H4a.seq check
>D03692A3_A6H4_2_EGFP-C2_E08_14_ABI24.ab1
    1 NNNNNNNNNN NNNCGNTCNC NTGGTCCTGC TGGAGTTCGT GANCGCCGCC GGGATCACTC
   61 TCGGCATGGA CGAGCTGTAC AAGTCCGGAC TCAGATCCAT GGCGAAGACC TACGATTACC
  121 TGTTCAAGCT GCTGCTGATC GGGGACTCGG GGGTAGGGAA GACCTGTGTC CTGTTCCGCT
  181 TCTCCGAGGA CGCCTTCAAC TCCACATTCA TCTCTACCAT AGGAATTGAC TTTAAAATTA
  241 GGACCATAGA GCTCGATGGC AAGAGGATTA AACTGCAGAT ATGGGACACG GCCGGCCAGG
  301 AGCGGTTTCG AACAATCACG ACAGCCTACT ACAGGGGTGC CATGGGTATC ATGCTGGTCT
  361 ACGACATTAC CAATGAGAAG TCCTTTGACA ACATCCGGAA TTGGATTCGG AACATTGAAG
  421 AGCATGCCTC TGCAGACGTG GAGAAGATGA TACTGGGGAA TAAGTGTGAT GTGAATGACA
  481 AGAGACAGGT GTCCAAGGAA CGGGGAGAAA AGCTGGCACT CGACTATGGG ATCAAGTTCA
  541 TGGAGACCAG TGCAAAGGCC AACATCAATG TGGAGAATGC ATTTTTCACT CTTGCCAGGG
  601 ATATCAAAGC AAAAATGGAC AAAAAATTGG AAGGGAACAG CCCGCAGGGG AGCAGCCATG
  661 GAGTCAAGAT CACAGTGGAG CAGCAGAAGA GGANCAGCTT CTTCCGGTGC AGTCTCCTGT
  721 GAAATCACTA GTGAATTCTG CAGTCGACGG TACCGCGGGC CCGGGATNCA CCGGATCTAG
  781 ATAACTGATC ATAATCAGCC ATACCACATT TGTAGAGGTT TTACTTGCTT TAAAAAACCT
  841 CCNACACCTC CNCCNGNNNC TGAAACATNN NNNGANNGNN ATTGNTGTTG TTNACTNGNT
  901 TATTGNANCT TATNANGGNT TNNNAATNAA GCAATANCAT CACAAATTTC NNNAATAAAG
961 CATTTTTTTT CNCTGNNNTN NNNNTGNNNN TTNGNNNNAA NNNNNNNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB14273_A6H4b.seq check
>D03692A3_A6H4_2_SV40polyA-R2_F08_17_ABI24.ab1
    1 NNNNNNNNNN NNNANNNNNN NGTTAANAAC NACNATTGCA TTCNTTTTAT GTTTCNGGTT
   61 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT
  121 GATTATGATC AGTTATCTAG ATCCGGTGGA TCCCGGGCCC GCGGTACCGT CGACTGCAGA
  181 ATTCACTAGT GATTTCACAG GAGACTGCAC CGGAAGAAGC TGGTCCTCTT CTGCTGCTCC
  241 ACTGTGATCT TGACTCCATG GCTGCTCCCC TGCGGGCTGT TCCCTTCCAA TTTTTTGTCC
  301 ATTTTTGCTT TGATATCCCT GGCAAGAGTG AAAAATGCAT TCTCCACATT GATGTTGGCC
  361 TTTGCACTGG TCTCCATGAA CTTGATCCCA TAGTCGAGTG CCAGCTTTTC TCCCCGTTCC
  421 TTGGACACCT GTCTCTTGTC ATTCACATCA CACTTATTCC CCAGTATCAT CTTCTCCACG
  481 TCTGCAGAGG CATGCTCTTC AATGTTCCGA ATCCAATTCC GGATGTTGTC AAAGGACTTC
  541 TCATTGGTAA TGTCGTAGAC CAGCATGATA CCCATGGCAC CCCTGTAGTA GGCTGTCGTG
  601 ATTGTTCGAA ACCGCTCCTG GCCGGCCGTG TCCCATATCT GCAGTTTAAT CCTCTTGCCA
  661 TCGAGCTCTA TGGTCCTAAT TTTAAAGTCA ATTCCTATGG TAGAGATGAA TGTGGAGTTG
  721 AAGGCGTCCT CGGAGAANCG GAACAGGACA CAGGTCTTCC CTACCCCCGA GTCCCCGATC
  781 AGCAGCAGCT TGAACAGGTA ATCGTANGTC TTCGCCATGG ATCTGANTCC GGANTTGTAC
  841 NGCTCGTCCA TGCCGANAGT GATCCCGGCG GCGGTCACGA ACTCCAGCNN GANCATGTGA
  901 TCGCGCTTCT CGTNGGGGNC TTTGCTCNNG NCGGNNTGGG NGNNCNNNNA NNGGTTGNCG
  961 GGNANCAGCA NGGGGNCNNC NNNNANGGGG GNGNNCNGNN NNNANNGGNN NNCGANNNGN
1021 NNNCNNNCNN NNNNNNANNN NNNNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,EGFP
Sequence file: RDB14273_A6H4c.seq check
>D03692A3_A6H4_2_CMV-Forward_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNTNGNN GCNNTGGGCG GTANGCGTGT ACGGTGGGAN GTCTATATAA
   61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGTCGCCA CCATGGTGAG
  121 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT
  181 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT
  241 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC
  301 CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA
  361 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA
  421 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG
  481 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA
  541 GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA
  601 GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA
  661 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAN
  721 CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGANAAGCGC GATCACATGG TCCTGCTGGA
  781 GTTCGTGACC GCCGCCGGGN TCACTCTCGG CATGGNCGAN CTGTACAANT CCGNACTCAG
  841 ATNCATGGCG AANANCTACN ATTACCNGTN CAAGCTGCTG CNNNTCNGGG NNTCNGGGGN
901 NNGGNAGANC NNNNNNCNNN TNNNNTTNNN NNNNNANGNC NTTNNNNTNN NNNNNNNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB14273_A6H4d.seq check
>D03692A3_A6H4_2_SV40pro_F_V2_H01_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGCTTTTGC AAGNNCGNTC ANGAGACAGG NTGAGGNNCG
   61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
  601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGANCGCTAT
  721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
  781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
  841 CTNCTTGACG ANTTCTTCTG ANCGGGNNTC TGGGGGTTCN AAATGNNCGA NCNAGCGACN
  901 CCCAACCNGN CNTCACNAGA NTNNNNNTNC NNNNNNGNCN TTCNNNNAAA NGNNGGGNTN
961 NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183. [link to RRC of NBRP]
original Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [link to RRC of NBRP]