Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-FLAG-mouse Rab10 (#RDB14272)

Expression vector of FLAG-tagged mouse Rab10.

Clone info. Expression vector driven by the EF1 promoter for FLAG-tagged mouse Rab10.
Comment Reference sequence is NM_016676. pEF-FLAG is delived from the pEF-BOS vector (J. Biol. Chem., 274 (44): 31421-31427., 1999. PMID 10531343) with FLAG-tag sequence at the upstream of the cloning site. Expression was confirmed by the depositor with immunoprecipitation.
Vector backbone pEF-FLAG (Plasmid)
Selectable markers Amp^r
Gene/insert name mouse Rab10 cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al., J. Biol. Chem. 277, 9212-9218, 2002); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuroda, T. S. et al., J. Biol. Chem. 277, 9212-9218, 2002); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14272 pEF-FLAG-mouse Rab10 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-FLAG-mouse Rab10 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14272).

Reference section:

Homma, Y., Fukuda, M., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183. [link to RRC of NBRP]
Kuroda, T.S., Fukuda, M., Ariga, H., Mikoshiba, K., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14272_A6H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: hGCSF_R (Pr0031)
Region: hGCSF pA, insert, FLAG, EF-1 alpha pro
Sequence file: RDB14272_A6H4a.seq check
>D03692A2_A6H4_1_hGCSF_R_D01_10_ABI24.ab1
    1 CGTGGGAATG CAACTCTCAG GGTCCCAGGT GGGGACCCTC ACTCTAGAGC GGCCGCTATG
   61 GTCGACCTGC AGGCGGCCGC GAATTCACTA GTGATTTCAG CAGCACTTGC TCTTCCAGCC
  121 CGTCACGCCT CCTCCACTGC TGATATCTAC GTTTTCACTG TTGGGTTCTT TTACAGGGGT
  181 CTTTCGGAGG ATGTCTTCAG CTAATGTGAG GAACGCCTTT TCGATGTTTA TATTTGCTTT
  241 TGCACTAGTC TCAAAAAACC TAATACCATG CTCCCTTGCA ATCTGTTCTC CTTTGCCTTT
  301 CGGTACAACT CTCTTGTCGT CCATGTCACA CTTGTTCCCT AGTAACATTC TTTCCACATC
  361 TTCATTGGCA TGCTCATCTA TGTTTCTAAG CCACTTGCTG ATGTTCTCAA AGCTTTTACC
  421 GTTGGTGATG TCATACACTA GCATGATGCC CATTGCTCCT CTGTAGTAGG AGGTTGTGAT
  481 GGTGTGAAAT CGCTCCTGGC CTGCTGTGTC CCATATCTGT AGCTTGATCT TCTTTCCTTG
  541 TAGTTCCACT GTTTTGATCT TAAAGTCTAT TCCTATGGTG GAAATAAAGG TGGTATTGAA
  601 GGCATCGTCC GAAAAACGAA AAAGGACGCA GGTCTTGCCC ACTCCCGAGT CCCCGATCAG
  661 GAGCAGCTTG AAAAGCAGGT CGTACGTCTT CTTCGCCATG GATCCCTTGT CGTCATCGTC
  721 CTTGTAGTCC ATGGTGGAAT CGAATTCCCG CGGCCGCTCT AGAGAATTCC TCACGACACC
  781 TGAAATGGAA GAAAAAAACT TTGAACCACT GTCTGAGGCT TGAGAATGAA CCAAGATCCA
  841 AACTCAAAAA GGGCAAATTC CAAGGAGAAT TACATCAAGT GCCAAGCTGG CCTAACTTCA
  901 GTCTCCACCC ACTCAGTGTG GGGAAACTCC ATCGCATAAA ACCCCTCCCC CCCAACCCTA
  961 AAGACGACGT ACTCCAAAAG CTCGAGAACT AATCGAGGTG CCCTGGACGG CGCCCCGGTA
 1021 CTCCGTGGAG TCACATGGAA GCGACGCTGG AGACGGAAAG CCTTTTTCCT TTGTGTGGTG
1081 ACTCACGAGC GCTCTCCGAA GCGCCGCGAT CATTCAATTT GGAGGCCTCC CCG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183. [link to RRC of NBRP]
original Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]