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pEF-FLAG-mouse Rab8A

Expression vector of FLAG-tagged mouse Rab8A.

Catalog number RDB14271
Resource name pEF-FLAG-mouse Rab8A
Clone info. Expression vector driven by the EF1 promoter for FLAG-tagged mouse Rab8A.
Reference sequence is S53270. pEF-FLAG is delived from the pEF-BOS vector (J. Biol. Chem., 274 (44): 31421-31427., 1999. PMID 10531343) with FLAG-tag sequence at the upstream of the cloning site. Expression was confirmed by the depositor with immunoprecipitation.
Vector backbone pEF-FLAG (Plasmid)
Selectable markers Amp^r
Gene/insert name mouse Rab8A cDNA
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Rab8a (NCBI Gene 17274) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al., J. Biol. Chem. 277, 9212-9218, 2002); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14271 pEF-FLAG-mouse Rab8A Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.
original Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Rab family protein (English text)

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.
original Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082.

2019.07.19

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