Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGL3/hISCU_p53 BR

Reporter plasmid of human ISCU gene.

Catalog number RDB14212
Resource name pGL3/hISCU_p53 BR
Clone info. Reporter plasmid of human ISCU gene. Promoter region corresponding to 108,563,001 to 108,563,300 of NC_000012.12 was cloned.
Comment Expression was confirmed by the depositor with reporter assay.
Vector backbone pGL3-Promoter (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name human ISCU genomic
Depositor|Developer Matsuda, Koichi |
Other clones in our bank
External Database
human ISCU
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Funauchi, Y. et al. Sci. Rep. 5: 16497, 2015).
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form           [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Funauchi, Y. et al. Sci. Rep. 5: 16497, 2015)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB14212 pGL3/hISCU_p53 BR DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Funauchi, Y., Regulation of iron homeostasis by the p53-ISCU pathway. Sci. Rep. 5: 16497 (2015). PMID 26560363.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14212_A6Frp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: Luc_R
          Sequence file: RDB14212_A6Fra.seq check
          >D03134A1_A6Fr_1_Luc_R_C11_08.ab1
              1 GTATTATTCA GGACAGGGCG TATCTCTTCA TAGCCTTATG CAGTTGCTCT CCAGCGGTTC
             61 CATCTTCCAG CGGATAGAAT GGCGCCGGGC CTTTCTTTAT GTTTTTGGCG TCTTCCATGG
            121 TGGCTTTACC AACAGTACCG GAATGCCAAG CTTTTTGCAA AAGCCTAGGC CTCCAAAAAA
            181 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT
            241 AAAAAAAATT AGTCAGCGAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG
            301 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA GATCGCAGAT
            361 CTCGAGCCCC AAAAACATTA CGCATGTTGG TTACAAGCCT GACTCCCCTA TGCACTCCTA
            421 AAGCAGTAAC AGAAACGGTC TCCTCGTGCT GGGGCCTATG TGCCAGGAAC CATGCCAGGA
            481 CTCCACGCAC AGTATCCTTT GTAAGGGACA GCTGAGAGCC CCCCATTTAC ACCTGGGGAA
            541 ACAGGTTCAG AGAGGCGCAG TGACTAGCCC AAGGTCATCT GGCCAGGGCA GCGGAGTGGC
            601 TGAAGCCGGA GCCCGCTCCG GGGGTGTGTC TGTCCCTGGA CTCAGAAAGA CAAGTCCCAA
            661 CAGGACCCTG GGCTAGCACG CGTAAGAGCT CGGTACCTAT CGATAGAGAA AATGTTCTGG
            721 CACCTGCACT TGCACTGGGG GACAGCCTAT TTTTGCTAGT TTGTTTTGTT TTCGTTTTGT
          781 TTTTGATGGG AGAGCCGTAT GTTTAGTACT ATCGATTTCA CACAAAAAAA CAAACACA
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Funauchi, Y., Regulation of iron homeostasis by the p53-ISCU pathway. Sci. Rep. 5: 16497 (2015). PMID 26560363.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl