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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGL3/hISCU_p53 BR (#RDB14212)

Reporter plasmid of human ISCU gene.

Clone info. Reporter plasmid of human ISCU gene. Promoter region corresponding to 108,563,001 to 108,563,300 of NC_000012.12 was cloned.
Comment Expression was confirmed by the depositor with reporter assay.
Vector backbone pGL3-Promoter (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name human ISCU genomic
Depositor|Developer Matsuda, Koichi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Funauchi, Y. et al. Sci. Rep. 5: 16497, 2015).
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Funauchi, Y. et al. Sci. Rep. 5: 16497, 2015)。

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RDB14212 pGL3/hISCU_p53 BR DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL3/hISCU_p53 BR was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14212).

Reference section:

Funauchi, Y., Tanikawa, C., Yi Lo, P.H., Mori, J., Daigo, Y., Takano, A., Miyagi, Y., Okawa, A., Nakamura, Y., Matsuda, K., Regulation of iron homeostasis by the p53-ISCU pathway. Sci. Rep. 5: 16497 (2015). PMID 26560363. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14212_A6Frp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Luc_R
Sequence file: RDB14212_A6Fra.seq check
>D03134A1_A6Fr_1_Luc_R_C11_08.ab1
    1 GTATTATTCA GGACAGGGCG TATCTCTTCA TAGCCTTATG CAGTTGCTCT CCAGCGGTTC
   61 CATCTTCCAG CGGATAGAAT GGCGCCGGGC CTTTCTTTAT GTTTTTGGCG TCTTCCATGG
  121 TGGCTTTACC AACAGTACCG GAATGCCAAG CTTTTTGCAA AAGCCTAGGC CTCCAAAAAA
  181 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT
  241 AAAAAAAATT AGTCAGCGAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG
  301 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA GATCGCAGAT
  361 CTCGAGCCCC AAAAACATTA CGCATGTTGG TTACAAGCCT GACTCCCCTA TGCACTCCTA
  421 AAGCAGTAAC AGAAACGGTC TCCTCGTGCT GGGGCCTATG TGCCAGGAAC CATGCCAGGA
  481 CTCCACGCAC AGTATCCTTT GTAAGGGACA GCTGAGAGCC CCCCATTTAC ACCTGGGGAA
  541 ACAGGTTCAG AGAGGCGCAG TGACTAGCCC AAGGTCATCT GGCCAGGGCA GCGGAGTGGC
  601 TGAAGCCGGA GCCCGCTCCG GGGGTGTGTC TGTCCCTGGA CTCAGAAAGA CAAGTCCCAA
  661 CAGGACCCTG GGCTAGCACG CGTAAGAGCT CGGTACCTAT CGATAGAGAA AATGTTCTGG
  721 CACCTGCACT TGCACTGGGG GACAGCCTAT TTTTGCTAGT TTGTTTTGTT TTCGTTTTGT
781 TTTTGATGGG AGAGCCGTAT GTTTAGTACT ATCGATTTCA CACAAAAAAA CAAACACA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Funauchi, Y., Regulation of iron homeostasis by the p53-ISCU pathway. Sci. Rep. 5: 16497 (2015). PMID 26560363. [link to RRC of NBRP]