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human MLC20 WT

Shuttle vector to generate recombinant adenovirus expressing human MYL12B (wild type).

Catalog number RDB14158
Resource name human MLC20 WT
Clone info. Shuttle vector to generate recombinant adenovirus expressing human MYL12B (wild type).
Expression was confirmed by the depositor with immunoblot and microphotographs.
Vector backbone pAxCAwtit (Cosmid, use packaging extracts for transformation)
Size of vector backbone 45 kb
Selectable markers Amp^r
Gene/insert name Human MYL12B cDNA
Depositor Hirano, Katsuya |
 
Other clones in our bank human MYL12B (NCBI Gene 103910) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hirano, M. et al., Sci. Rep. 6:20989, 2016)
(Japanese text [open/close])
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14158 human MLC20 WT DNA solution

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14158_A6Epp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB14158_A6Epa.seq
>D03455A1_A6Ep_pAxCA_F1_A09_03.ab1
    1 AAAGGGGGGT ATCTTCAAAC CTGGTTCTTG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA
   61 AACGTGCTGG TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTGATTTA TCGATTTGCG
  121 GGCCCCACCA TGTCGAGCAA AAAGGCAAAG ACCAAGACCA CCAAGAAGCG CCCTCAGCGT
  181 GCAACATCCA ATGTGTTTGC CATGTTTGAC CAGTCACAGA TTCAGGAGTT CAAAGAGGCC
  241 TTCAACATGA TTGATCAGAA CAGAGATGGC TTCATCGACA AGGAAGATTT GCATGATATG
  301 CTTGCTTCTC TAGGGAAGAA TCCCACTGAT GCATACCTTG ATGCCATGAT GAATGAGGCC
  361 CCAGGGCCCA TCAATTTCAC CATGTTCCTG ACCATGTTTG GTGAGAAGTT AAATGGCACA
  421 GATCCTGAAG ATGTCATCAG AAACGCCTTT GCTTGCTTTG ATGAAGAAAC AACAGGCACC
  481 ATTCAGGAAG ATTACCTAAG AGAGCTGCTG ACAACCATGG GGGATCGGTT TACAGATGAG
  541 GAAGTGGATG AGCTGTACAG AGAAGCACCT ATTGACAAAA AGGGGAATTT CAATTACATC
  601 GAGTTCACAC GCATCCTGAA ACATGGAGCC AAAGACAAAG ATGACTGAGA GAACTTTAGC
  661 TAAAAGGGGA TCAAATTAAT TAAACTAGTC CTAGAATCGA TAAATCAATT CACTCCTCAG
  721 GTGCAGGCTG CCTATCAAAG GTGGTGGCTG GTGTGGCCCA ATGCCCTGGC TCACAAATAC
  781 CCACTGAGAT CTTTTTTCCC TCTGCCAAAA ATTATGGGGG ACATCATGAA GCCCCTTGAC
  841 CATCTGACTT CTGGCTAATA AAAGGAAATT TATTTTTCAT TTGCAATAAT GTGTTGGGAA
  901 TTTTTTTGGT GTCTTCTCAC TTCGGAAGGG ACATAATGGG GAGGGCCAAA TCATTTAAAA
  961 CATCCCAAAT GAGTATTTTG GTTTAAAGTT TTGGCAACAT ATGCCCCATA TGCTGGCCTG
 1021 CCATGAACAA AGGGTTGGGC TATAAAGAAG GTCATCCAGG TATATATGAA AACAGCCCCC
 1081 CTGGCTGGTA CCATTTCCTT AATTCCCATA GAAAAAGCCC TTTGAAATAT GGAGGGTTAG
1141 CAATTTTATC TTTTTTAGAA TATCATGTTG CGCTATCT
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

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References

original Hirano, M., Myosin di-phosphorylation and peripheral actin bundle formation as initial events during endothelial barrier disruption. Sci. Rep. 6: 20989 (2016). PMID 26863988.

2017.10.19

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