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pCMV-TUBA1A myc-R402C (#RDB14147)

Expression vector of Myc-tagged human TUBA1A mutant, R402C.

Clone info. Expression vector of Myc-tagged human TUBA1A mutant, R402C.
Comment Expression was confirmed by the depositor with immunofluorescence.
Vector backbone pCMV-Tag 4 (Plasmid)
Size of vector backbone 4.3 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human tubulin alpha 1a cDNA
Depositor|Developer Kurahashi, Hiroki | Yokoi, Setsuri | Tsutsumi, Makiko |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yokoi, S. et al., Sci. Rep. 5:15165, 2015)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yokoi, S. et al., Sci. Rep. 5:15165, 2015)。

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RDB14147 pCMV-TUBA1A myc-R402C DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMV-TUBA1A myc-R402C was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14147).

Reference section:

Yokoi, S., Ishihara, N., Miya, F., Tsutsumi, M., Yanagihara, I., Fujita, N., Yamamoto, H., Kato, M., Okamoto, N., Tsunoda, T., Yamasaki, M., Kanemura, Y., Kosaki, K., Kojima, S., Saitoh, S., Kurahashi, H., Natsume, J., TUBA1A mutation can cause a hydranencephaly-like severe form of cortical dysgenesis. Sci. Rep. 5: 15165 (2015). PMID 26493046. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14147_A6Dpp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB14147_A6Dpa.seq check
>D03180A8_A6Dp_1_CMV-Forward_D11_11.ab1
    1 CTTCACTCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCGATTAC GCCAAGCTCG AAATTAACCC
  121 TCACTAAAGG GAACAAAAGC TGGAGCTCCA CCGCGGTGGC GGCCGCCATG CGTGAGTGCA
  181 TCTCCATCCA CGTTGGCCAG GCTGGTGTCC AGATTGGCAA TGCCTGCTGG GAGCTCTACT
  241 GCCTGGAACA CGGCATCCAG CCCGATGGCC AGATGCCAAG TGACAAGACC ATTGGGGGAG
  301 GAGATGATTC CTTCAACACC TTCTTCAGTG AGACGGGGGC TGGCAAGCAT GTGCCCCGGG
  361 CAGTGTTTGT AGACTTGGAA CCCACAGTCA TTGATGAAGT TCGCACTGGC ACCTACCGCC
  421 AGCTCTTCCA CCCTGAGCAA CTTATCACAG GCAAGGAAGA TGCTGCCAAT AACTATGCCC
  481 GAGGGCACTA CACCATTGGC AAGGAGATCA TTGACCTCGT GTTGGACCGA ATTCGCAAGC
  541 TGGCCGACCA GTGCACGGGT CTCCAGGGCT TCTTGGTTTT CCACAGCTTT GGTGGGGGAA
  601 CTGGTTCTGG GTTCACCTCC CTGCTCATGG AACGTCTCTC AGTTGATTAT GGCAAGAAGT
  661 CCAAGCTGGA GTTCTCTATT TACCCGGCGC CCCAGGTTTC CACAGCTGTA GTTGAGCCCT
  721 ACAACTCCAT CCTCACCACC CACACCACCC TGGAGCACTC TGATTGTGCC TTCATGGTAG
  781 ACAATGAGGC CATCTATGAC ATCTGTCGTA GAAACCTCGA TATTGAGCGT CCAACCTATA
  841 CTAACCTGAA TAGGTTAATA GGTCAAATTG TGTCCTCCAT CACTGCTTCC CTGAGATTTG
  901 ATGGAGCCCT GAAATGTTGA CCTGACAGAA ATTCCAGACC AAACCTGGTG CCCTATCCCC
  961 CGCATCCCAC TTCCCTCTGG CCACATATGC CCCCTGTCCA TCCTCCTGCC TGAGAAAGCC
 1021 TACCAATGCA CCAGCTTTTC CTGTAGCCAG AAGATTCACC GATTGCCTTG CTTTGCAGCC
1081 ACCCCAA
//
Primer: T7_20bp
Sequence file: RDB14147_A6Dpb.seq check
>D03180A8_A6Dp_1_T7_20bp_E11_14.ab1
    1 TCATTACTGG TACTTATTAT TAAGGTACCG GGCCCTACAG ATCCTCTTCT GAGATGAGTT
   61 TTTGTTCCTC GAGGTCGACG GTATCGATAA GCTTGATATC GAATTCCTGC AGGTATTCCT
  121 CTCCTTCTTC CTCACCCTCT CCTTCAACAG AATCCACACC AACCTCCTCA TAATCCTTCT
  181 CAAGGGCAGC CATGTCCTCA CGGGCCTCTG AAAACTCACC TTCCTCCATC CCCTCCCCAA
  241 CGTACCAGTG AACAAAGGCA CATTTGGCAT ACATCAGGTC AAACTTGTGG TCCAGGCGAG
  301 CCCAGGCCTC AGCAATGGCT GTGGTGTTGC TCAGCATGCA CACAGCTCTC TGTACCTTGG
  361 CCAGGTCTCC ACCAGGCACC ACAGTGGGAG GCTGGTAGTT GATGCCAACC TTGAAGCCAG
  421 TGGGGCACCA ATCCACAAAC TGGATGGTAC GCTTGGTCTT GATGGTGGCA ATGGCAGCAT
  481 TGACATCTTT GGGAACCACG TCACCACGGT ACAACAGGCA GCAAGCCATG TATTTACCAT
  541 GGCGAGGGTC ACATTTCACC ATCTGGTTGG CTGGCTCAAA GCAAGCATTG GTGATCTCTG
  601 CTACAGAAAG CTGTTCATGG TAGGCTTTCT CAGCAGAGAT GACAGGGGCA TATGTGGCCA
  661 GAGGGAAGTG GATGCGGGGA TAGGGCACCA GGTTGGTCTG GAATTCTGTC AGGTCAACAT
  721 TCAGGGCTCC ATCAAATCTC AGGGAAGCAG TGATGGAGGA CACAATTTGA CCTATTAACC
  781 TATTCAGGTT AGTATAGGTT GGACGCTCAA TATCGAGGTT TCTACGACAG ATGTCATAGA
  841 TGGCCTCATT GTCTACCATG AAGGCACAAT CAGAGTGCTC CAGGGTGGTG TGGTGGTGAG
  901 ATGGAAGTTG TAGGGCTCAA CTACAGCTGT GGAACCTGGG GGCGCCCGGG TAAATAGAGA
  961 AACTCCCAGC TTTGTACTTT CTTTGCCATT AATTCCAACC TGAGAGGACG TTCCATTGAA
1021 GCAGGAGGTT CAACCCAGCA ACAGGATCCC CGATCGAAAA GGCTGATGA
//
Primer: SV40pro_F_V2
Sequence file: RDB14147_A6Dpc.seq check
>D03180A8_A6Dp_1_SV40pro_F_V2_F11_17.ab1
    1 GTAGAGCTTT TTGGAGGCTA GGCTTTTGCA AGATCGATCA AGAGACAGGA TGAGGATCGT
   61 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  121 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  181 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  241 AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  301 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  361 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  421 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  481 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  541 ACGAAGAACA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC
  601 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  661 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
  721 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAACT TGGCGGCGAA TGGGCTGACC
  781 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
  841 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGGTTCGA ATGACCGACC AAGCGACGCC
  901 CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCCTT CTATGAAGGG TTGGGCTTCG
  961 GAATCGTTTT CCCGGGACGC CCGGCTGGGA ATGATCTCAG CGCGGGAATC TCATGCTGAA
1021 GTCTTCGGTC CACCTAGGGA AGGCTAACTG CAAACACCGG A
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yokoi, S., TUBA1A mutation can cause a hydranencephaly-like severe form of cortical dysgenesis. Sci. Rep. 5: 15165 (2015). PMID 26493046. [link to RRC of NBRP]