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pCMV-TUBA1A myc-C25F (#RDB14146)

Expression vector of Myc-tagged human TUBA1A mutant, C64F.

Clone info. Expression vector of Myc-tagged human TUBA1A mutant, C64F.
Comment Expression was confirmed by the depositor with immunofluorescence.
Vector backbone pCMV-Tag 4 (Plasmid)
Size of vector backbone 4.3 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human tubulin alpha 1a cDNA
Depositor|Developer Kurahashi, Hiroki | Yokoi, Setsuri | Tsutsumi, Makiko |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yokoi, S. et al., Sci. Rep. 5:15165, 2015)
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yokoi, S. et al., Sci. Rep. 5:15165, 2015)。

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RDB14146 pCMV-TUBA1A myc-C25F DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMV-TUBA1A myc-C25F was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14146).

Reference section:

Yokoi, S., Ishihara, N., Miya, F., Tsutsumi, M., Yanagihara, I., Fujita, N., Yamamoto, H., Kato, M., Okamoto, N., Tsunoda, T., Yamasaki, M., Kanemura, Y., Kosaki, K., Kojima, S., Saitoh, S., Kurahashi, H., Natsume, J., TUBA1A mutation can cause a hydranencephaly-like severe form of cortical dysgenesis. Sci. Rep. 5: 15165 (2015). PMID 26493046. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14146_A6Dpp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB14146_A6Dpa.seq check
>D03180A7_A6Dp_1_CMV-Forward_A11_02.ab1
    1 ATTAACTCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCGATTAC GCCAAGCTCG AAATTAACCC
  121 TCACTAAAGG GAACAAAAGC TGGAGCTCCA CCGCGGTGGC GGCCGCCATG CGTGAGTGCA
  181 TCTCCATCCA CGTTGGCCAG GCTGGTGTCC AGATTGGCAA TGCCTGCTGG GAGCTCTACT
  241 TCCTGGAACA CGGCATCCAG CCCGATGGCC AGATGCCAAG TGACAAGACC ATTGGGGGAG
  301 GAGATGATTC CTTCAACACC TTCTTCAGTG AGACGGGGGC TGGCAAGCAT GTGCCCCGGG
  361 CAGTGTTTGT AGACTTGGAA CCCACAGTCA TTGATGAAGT TCGCACTGGC ACCTACCGCC
  421 AGCTCTTCCA CCCTGAGCAA CTTATCACAG GCAAGGAAGA TGCTGCCAAT AACTATGCCC
  481 GAGGGCACTA CACCATTGGC AAGGAGATCA TTGACCTCGT GTTGGACCGA ATTCGCAAGC
  541 TGGCCGACCA GTGCACGGGT CTCCAGGGCT TCTTGGTTTT CCACAGCTTT GGTGGGGGAA
  601 CTGGTTCTGG GTTCACCTCC CTGCTCATGG AACGTCTCTC AGTTGATTAT GGCAAGAAGT
  661 CCAAGCTGGA GTTCTCTATT TACCCGGCGC CCCAGGTTTC CACAGCTGTA GTTGAGCCCT
  721 ACAACTCCAT CCTCACCACC CACACCACCC TGGAGCACTC TGATTGTGCC TTCATGGTAG
  781 ACAATGAGGC CATCTATGAC ATCTGTCGTA GAAACCTCGA TATTGAGCGT CCAACCTATA
  841 CTAACCTGAA TAGGTTAATA GGTCAAATTG TGTCCTCCAT CACTGCTTCC CTGAGATTTG
  901 ATGGAGCCCT GAAATGTTGA CCCTGACAGA ATTTCCAGAC CAAACCCTGG TGCCTATCCC
  961 CGCATCCACT TTCCCTCTGG CACATATGCC CCTGTCATCT CTGCCTGAGA AAGTCTACCC
 1021 ATGGAACCAG CCTTTCCTGG TAAGCAGAGA TCGCGAAGTG CTGCCGTTGA AGTCAGCCGA
1081 AACCAGAATG
//
Primer: T7_20bp
Sequence file: RDB14146_A6Dpb.seq check
>D03180A7_A6Dp_1_T7_20bp_B11_05.ab1
    1 TATTACTGGT ACTTATTATT AAGGTACCGG GCCCTACAGA TCCTCTTCTG AGATGAGTTT
   61 TTGTTCCTCG AGGTCGACGG TATCGATAAG CTTGATATCG AATTCCTGCA GGTATTCCTC
  121 TCCTTCTTCC TCACCCTCTC CTTCAACAGA ATCCACACCA ACCTCCTCAT AATCCTTCTC
  181 AAGGGCAGCC ATGTCCTCAC GGGCCTCTGA AAACTCACCT TCCTCCATCC CCTCCCCAAC
  241 GTACCAGTGA ACAAAGGCAC GTTTGGCATA CATCAGGTCA AACTTGTGGT CCAGGCGAGC
  301 CCAGGCCTCA GCAATGGCTG TGGTGTTGCT CAGCATGCAC ACAGCTCTCT GTACCTTGGC
  361 CAGGTCTCCA CCAGGCACCA CAGTGGGAGG CTGGTAGTTG ATGCCAACCT TGAAGCCAGT
  421 GGGGCACCAA TCCACAAACT GGATGGTACG CTTGGTCTTG ATGGTGGCAA TGGCAGCATT
  481 GACATCTTTG GGAACCACGT CACCACGGTA CAACAGGCAG CAAGCCATGT ATTTACCATG
  541 GCGAGGGTCA CATTTCACCA TCTGGTTGGC TGGCTCAAAG CAAGCATTGG TGATCTCTGC
  601 TACAGAAAGC TGTTCATGGT AGGCTTTCTC AGCAGAGATG ACAGGGGCAT ATGTGGCCAG
  661 AGGGAAGTGG ATGCGGGGAT AGGGCACCAG GTTGGTCTGG AATTCTGTCA GGTCAACATT
  721 CAGGGCTCCA TCAAATCTCA GGGAAGCAGT GATGGAGGAC ACAATTTGAC CTATTAACCT
  781 ATTCAGGTTA GTATAGGTTG GACGCTCAAT ATCGAGGTTT CTACGACAGA TGTCATAGAT
  841 GGCCTCATTG TCTACCATGA AAGGCACAAT CAGAGTGCTC CAGGGTGGTG TGGTGTGAGA
  901 TGGGGGGAAG TTTGTGTAGG CTTCAACCTA ACAGGCCTGT GGAAAACCTG GGGGCGCCCG
  961 GGGTAAATAG AAGAAACTCC CAGCTTTGGT ACTTCTTTGT CATTATTCCA CCTGAGAGGA
1021 CGGATGCAAT GAAGCCAAGG AGGATGCACT CAGATCAGCT TCCCCAACCA AAGTCT
//
Primer: SV40pro_F_V2
Sequence file: RDB14146_A6Dpc.seq check
>D03180A7_A6Dp_1_SV40pro_F_V2_C11_08.ab1
    1 GGGGGCTTTT TGGAGCCTAG GCTTTTGCAA GATCGATCAA GAGACAGGAT GAGGATCGTT
   61 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
  121 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
  181 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
  241 ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
  301 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
  361 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
  421 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
  481 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
  541 CGAAGAACAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC
  601 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
  661 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
  721 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAACTT GGCGGCGAAT GGGCTGACCG
  781 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT
  841 TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGGTTCGAA ATGACCGACC AAGCGACGCC
  901 CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCCTT CTATGAAAGG TTGGGCTTCG
  961 GAATCGTTTT CCGGGACGCC CGGCTGGGAT GAATCTCAGC GCGGGGATCT CATGCCTGAA
1021 GTCCTCCGCC GATCCTTAGG GAAGCTTAAC CTGAACACG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yokoi, S., TUBA1A mutation can cause a hydranencephaly-like severe form of cortical dysgenesis. Sci. Rep. 5: 15165 (2015). PMID 26493046. [link to RRC of NBRP]