RDB14144 - pCMV-TUBA1A myc-WT

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pCMV-TUBA1A myc-WT (#RDB14144)

Expression vector of Myc-tagged human TUBA1A (wild type).

Clone info.	Expression vector of Myc-tagged human TUBA1A (wild type).
Comment	Expression was confirmed by the depositor with immunofluorescence.
Vector backbone	pCMV-Tag 4 (Plasmid)
Size of vector backbone	4.3 kb
Selectable markers	Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name	human tubulin alpha 1a cDNA
Depositor\|Developer	Kurahashi, Hiroki \| Yokoi, Setsuri \| Tsutsumi, Makiko \|

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yokoi, S. et al., Sci. Rep. 5:15165, 2015)

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yokoi, S. et al., Sci. Rep. 5:15165, 2015)

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yokoi, S. et al., Sci. Rep. 5:15165, 2015)。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14144	pCMV-TUBA1A myc-WT	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMV-TUBA1A myc-WT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14144).

Reference section:

Yokoi, S., Ishihara, N., Miya, F., Tsutsumi, M., Yanagihara, I., Fujita, N., Yamamoto, H., Kato, M., Okamoto, N., Tsunoda, T., Yamasaki, M., Kanemura, Y., Kosaki, K., Kojima, S., Saitoh, S., Kurahashi, H., Natsume, J., TUBA1A mutation can cause a hydranencephaly-like severe form of cortical dysgenesis. Sci. Rep. 5: 15165 (2015). PMID 26493046. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14144_A6Dpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB14144_A6Dpa.seq
>D03180A5_A6Dp_2_CMV-Forward_A10_01.ab1 1 ATTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGATTA CGCCAAGCTC GAAATTAACC 121 CTCACTAAAG GGAACAAAAG CTGGAGCTCC ACCGCGGTGG CGGCCGCCAT GCGTGAGTGC 181 ATCTCCATCC ACGTTGGCCA GGCTGGTGTC CAGATTGGCA ATGCCTGCTG GGAGCTCTAC 241 TGCCTGGAAC ACGGCATCCA GCCCGATGGC CAGATGCCAA GTGACAAGAC CATTGGGGGA 301 GGAGATGATT CCTTCAACAC CTTCTTCAGT GAGACGGGGG CTGGCAAGCA TGTGCCCCGG 361 GCAGTGTTTG TAGACTTGGA ACCCACAGTC ATTGATGAAG TTCGCACTGG CACCTACCGC 421 CAGCTCTTCC ACCCTGAGCA ACTTATCACA GGCAAGGAAG ATGCTGCCAA TAACTATGCC 481 CGAGGGCACT ACACCATTGG CAAGGAGATC ATTGACCTCG TGTTGGACCG AATTCGCAAG 541 CTGGCCGACC AGTGCACGGG TCTCCAGGGC TTCTTGGTTT TCCACAGCTT TGGTGGGGGA 601 ACTGGTTCTG GGTTCACCTC CCTGCTCATG GAACGTCTCT CAGTTGATTA TGGCAAGAAG 661 TCCAAGCTGG AGTTCTCTAT TTACCCGGCG CCCCAGGTTT CCACAGCTGT AGTTGAGCCC 721 TACAACTCCA TCCTCACCAC CCACACCACC CTGGAGCACT CTGATTGTGC CTTCATGGTA 781 GACAATGAGG CCATCTATGA CATCTGTCGT AGAAACCTCG ATATTGAGCG TCCAACCTAT 841 ACTAACCTGA ATAGGTTAAT AGGTCAAATT GTGTCCTCCA TCACTGCTTC CCTGAGATTT 901 GATGGGAGCC CTGAAATGTT TGACCTGACA GAATTTCCAG ACCAAACCCT CTGCCCCTAT 961 CCCCCGCATC CACTTTCCCT CTGACCACAT ATGCCCCCTG TCATCTCTGG CTGGAGAAAG 1021 GCCTTACCCA TGAACCAGGC CTTTCTGGTA AGCAGAAGAT CACTATGCCG TGCATTGAAG 1081 CAAGCCAACC AGAATT //
Primer: T7_20bp Sequence file: RDB14144_A6Dpb.seq
>D03180A5_A6Dp_2_T7_20bp_B10_04.ab1 1 TAATTACTGG GTACTTATTA TTAAGGTACC GGGCCCTACA GATCCTCTTC TGAGATGAGT 61 TTTTGTTCCT CGAGGTCGAC GGTATCGATA AGCTTGATAT CGAATTCCTG CAGGTATTCC 121 TCTCCTTCTT CCTCACCCTC TCCTTCAACA GAATCCACAC CAACCTCCTC ATAATCCTTC 181 TCAAGGGCAG CCATGTCCTC ACGGGCCTCT GAAAACTCAC CTTCCTCCAT CCCCTCCCCA 241 ACGTACCAGT GAACAAAGGC ACGTTTGGCA TACATCAGGT CAAACTTGTG GTCCAGGCGA 301 GCCCAGGCCT CAGCAATGGC TGTGGTGTTG CTCAGCATGC ACACAGCTCT CTGTACCTTG 361 GCCAGGTCTC CACCAGGCAC CACAGTGGGA GGCTGGTAGT TGATGCCAAC CTTGAAGCCA 421 GTGGGGCACC AATCCACAAA CTGGATGGTA CGCTTGGTCT TGATGGTGGC AATGGCAGCA 481 TTGACATCTT TGGGAACCAC GTCACCACGG TACAACAGGC AGCAAGCCAT GTATTTACCA 541 TGGCGAGGGT CACATTTCAC CATCTGGTTG GCTGGCTCAA AGCAAGCATT GGTGATCTCT 601 GCTACAGAAA GCTGTTCATG GTAGGCTTTC TCAGCAGAGA TGACAGGGGC ATATGTGGCC 661 AGAGGGAAGT GGATGCGGGG ATAGGGCACC AGGTTGGTCT GGAATTCTGT CAGGTCAACA 721 TTCAGGGCTC CATCAAATCT CAGGGAAGCA GTGATGGAGG ACACAATTTG ACCTATTAAC 781 CTATTCAGGT TAGTATAGGT TGGACGCTCA ATATCGAGGT TTCTACGACA GATGTCATAG 841 ATGGCCTCAT TGTCTACCAT GAAGGCACAA TCAGAGTGCT CCAGGGTGGT GTGGGTGGTG 901 AGGAATGGGA GGTTGTA //
Primer: SV40pro_F_V2 Sequence file: RDB14144_A6Dpc.seq
>D03180A5_A6Dp_2_SV40pro_F_V2_C10_07.ab1 1 GTGGGGCTTT TTTGGAGCTA GGCTTTTGCA AGATCGATCA AGAGACAGGA TGAGGATCGT 61 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC 121 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC 181 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG 241 AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG 301 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG 361 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG 421 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC 481 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG 541 ACGAAGAACA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC 601 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG 661 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC 721 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAACT TGGCGGCGAA TGGGGCTGAC 781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGGTTCG AAATGACCGA CCAAGCGACG 901 CCCAACCTGC CATCACGAGA TTTTCGATTC CACCGCCCGC CTTCTATGAA AGGGTTGGGG 961 CTTCGGAATC GTTTTCCGGG ACGCCGGCTT GGATGATCCC TCCAGCGGCG GGGATCCTCA 1021 TGCTGAGTCA TCCACCAACC CCTAGGGGAA GCTAACCTGG AAACACGGTA TGGAGAACGA 1081 AT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Yokoi, S., TUBA1A mutation can cause a hydranencephaly-like severe form of cortical dysgenesis. Sci. Rep. 5: 15165 (2015). PMID 26493046. [link to RRC of NBRP]

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human tubulin alpha 1a

External Database
human tubulin alpha 1a

Reference sequence