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pCMV-TUBA1A flag-R402C (#RDB14143)

Expression vector of FLAG-tagged human TUBA1A mutant, R402C.

Clone info. Expression vector of FLAG-tagged human TUBA1A mutant, R402C.
Comment Expression was confirmed by the depositor with immunofluorescence.
Vector backbone pCMV-Tag 4 (Plasmid)
Size of vector backbone 4.3 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human tubulin alpha 1a cDNA
Depositor|Developer Kurahashi, Hiroki | Yokoi, Setsuri | Tsutsumi, Makiko |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yokoi, S. et al., Sci. Rep. 5:15165, 2015)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yokoi, S. et al., Sci. Rep. 5:15165, 2015)。

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RDB14143 pCMV-TUBA1A flag-R402C DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMV-TUBA1A flag-R402C was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14143).

Reference section:

Yokoi, S., Ishihara, N., Miya, F., Tsutsumi, M., Yanagihara, I., Fujita, N., Yamamoto, H., Kato, M., Okamoto, N., Tsunoda, T., Yamasaki, M., Kanemura, Y., Kosaki, K., Kojima, S., Saitoh, S., Kurahashi, H., Natsume, J., TUBA1A mutation can cause a hydranencephaly-like severe form of cortical dysgenesis. Sci. Rep. 5: 15165 (2015). PMID 26493046. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14143_A6Dpp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB14143_A6Dpa.seq check
>D03180A4_A6Dp_3_CMV-Forward_D06_12.ab1
    1 ATTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGATTA CGCCAAGCTC GAAATTAACC
  121 CTCACTAAAG GGAACAAAAG CTGGAGCTCC ACCGCGGTGG CGGCCGCCAT GCGTGAGTGC
  181 ATCTCCATCC ACGTTGGCCA GGCTGGTGTC CAGATTGGCA ATGCCTGCTG GGAGCTCTAC
  241 TGCCTGGAAC ACGGCATCCA GCCCGATGGC CAGATGCCAA GTGACAAGAC CATTGGGGGA
  301 GGAGATGATT CCTTCAACAC CTTCTTCAGT GAGACGGGGG CTGGCAAGCA TGTGCCCCGG
  361 GCAGTGTTTG TAGACTTGGA ACCCACAGTC ATTGATGAAG TTCGCACTGG CACCTACCGC
  421 CAGCTCTTCC ACCCTGAGCA ACTTATCACA GGCAAGGAAG ATGCTGCCAA TAACTATGCC
  481 CGAGGGCACT ACACCATTGG CAAGGAGATC ATTGACCTCG TGTTGGACCG AATTCGCAAG
  541 CTGGCCGACC AGTGCACGGG TCTCCAGGGC TTCTTGGTTT TCCACAGCTT TGGTGGGGGA
  601 ACTGGTTCTG GGTTCACCTC CCTGCTCATG GAACGTCTCT CAGTTGATTA TGGCAAGAAG
  661 TCCAAGCTGG AGTTCTCTAT TTACCCGGCG CCCCAGGTTT CCACAGCTGT AGTTGAGCCC
  721 TACAACTCCA TCCTCACCAC CCACACCACC CTGGAGCACT CTGATTGTGC CTTCATGGTA
  781 GACAATGAGG CCATCTATGA CATCTGTCGT AGAAACCTCG ATATTGAGCG TCCAACCTAT
  841 ACTAACCTGA ATAGGTTAAT AGGTCAAATT GTGTCCTCCA TCACTGCTTC CCTGAGATTT
  901 GATGGAGCCC TGAATGTTGA CCTGACAGAA TTTCCAGACC AACCTGGTGC CCTATCCCCC
  961 GCATCCACTT TCCCTCTGGG CCACATATGC CCCCTGTCAT CCTCCTGCCT GGAGAAAGGC
 1021 CTACCCATGA ACAGGCTTTC CTGGTAAGCC AGAGATCACT ATGACGTTGC CTTTGAAGTC
1081 AAGGTGAACC AAGGAATTG
//
Primer: T7_20bp
Sequence file: RDB14143_A6Dpb.seq check
>D03180A4_A6Dp_3_T7_20bp_H06_24.ab1
    1 TCATTACTGG GTACTTATTA TTAAGGTACC GGGCCCTACT TATCGTCGTC ATCCTTGTAA
   61 TCCTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT CCTGCAGGTA TTCCTCTCCT
  121 TCTTCCTCAC CCTCTCCTTC AACAGAATCC ACACCAACCT CCTCATAATC CTTCTCAAGG
  181 GCAGCCATGT CCTCACGGGC CTCTGAAAAC TCACCTTCCT CCATCCCCTC CCCAACGTAC
  241 CAGTGAACAA AGGCACATTT GGCATACATC AGGTCAAACT TGTGGTCCAG GCGAGCCCAG
  301 GCCTCAGCAA TGGCTGTGGT GTTGCTCAGC ATGCACACAG CTCTCTGTAC CTTGGCCAGG
  361 TCTCCACCAG GCACCACAGT GGGAGGCTGG TAGTTGATGC CAACCTTGAA GCCAGTGGGG
  421 CACCAATCCA CAAACTGGAT GGTACGCTTG GTCTTGATGG TGGCAATGGC AGCATTGACA
  481 TCTTTGGGAA CCACGTCACC ACGGTACAAC AGGCAGCAAG CCATGTATTT ACCATGGCGA
  541 GGGTCACATT TCACCATCTG GTTGGCTGGC TCAAAGCAAG CATTGGTGAT CTCTGCTACA
  601 GAAAGCTGTT CATGGTAGGC TTTCTCAGCA GAGATGACAG GGGCATATGT GGCCAGAGGG
  661 AAGTGGATGC GGGGATAGGG CACCAGGTTG GTCTGGAATT CTGTCAGGTC AACATTCAGG
  721 GCTCCATCAA ATCTCAGGGA AGCAGTGATG GAGGACACAA TTTGACCTAT TAACCTATTC
  781 AGGTTAGTAT AGGTTGGACG CTCAATATCG AGGTTTCTAC GACAGATGTC ATAGATGGCC
  841 TCATTGTCTA CCATGAAGGC ACAATCAGAG TGCTCCAGGG TGGTGTGGGT GGTGAGGAAT
901 GGGAGTTTGT
//
Primer: SV40pro_F_V2
Sequence file: RDB14143_A6Dpc.seq check
>D03180A4_A6Dp_3_SV40pro_F_V2_D08_11.ab1
    1 GTGCGGCTTT TTTGGGAGCC TAGGCTTTTG CAAGATCGAT CAAGAGACAG GATGAGGATC
   61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  541 GGACGAAGAA CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
  601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAA CTTGGCGGCG AATGGGCTGA
  781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  841 CCTTCTTGAC GAGTTCTTCT GAGCGGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC
  901 GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC CTTCTATGAA AGGTTGGGCT
  961 TCGGAATCGT TTTTCCGGGA CGCCCGGCTG GATGATCCCT CCAGCGGCGG GGATCTCATG
 1021 CCTGGAAGTT TCATCGCCCA ACCCTAGGGG AGCCTACCTG AAACACGGAA GGAGACGATT
1081 ACCCGGA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yokoi, S., TUBA1A mutation can cause a hydranencephaly-like severe form of cortical dysgenesis. Sci. Rep. 5: 15165 (2015). PMID 26493046. [link to RRC of NBRP]