Expression vector of FLAG-tagged human TUBA1A mutant, R402C.
Clone info. | Expression vector of FLAG-tagged human TUBA1A mutant, R402C. |
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Comment | Expression was confirmed by the depositor with immunofluorescence. |
Vector backbone | pCMV-Tag 4 (Plasmid) |
Size of vector backbone | 4.3 kb |
Selectable markers | Kan^r (E. coli), Neo^r (mammalian cells). |
Gene/insert name | human tubulin alpha 1a cDNA |
Depositor|Developer | Kurahashi, Hiroki | Yokoi, Setsuri | Tsutsumi, Makiko | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yokoi, S. et al., Sci. Rep. 5:15165, 2015) |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yokoi, S. et al., Sci. Rep. 5:15165, 2015)。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB14143 | pCMV-TUBA1A flag-R402C | DNA solution |
Materials & Methods section:
The pCMV-TUBA1A flag-R402C was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14143). |
Reference section:
Yokoi, S., Ishihara, N., Miya, F., Tsutsumi, M., Yanagihara, I., Fujita, N., Yamamoto, H., Kato, M., Okamoto, N., Tsunoda, T., Yamasaki, M., Kanemura, Y., Kosaki, K., Kojima, S., Saitoh, S., Kurahashi, H., Natsume, J., TUBA1A mutation can cause a hydranencephaly-like severe form of cortical dysgenesis. Sci. Rep. 5: 15165 (2015). PMID 26493046. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB14143_A6Dpp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: CMV-Forward Sequence file: RDB14143_A6Dpa.seq |
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>D03180A4_A6Dp_3_CMV-Forward_D06_12.ab1 1 ATTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGATTA CGCCAAGCTC GAAATTAACC 121 CTCACTAAAG GGAACAAAAG CTGGAGCTCC ACCGCGGTGG CGGCCGCCAT GCGTGAGTGC 181 ATCTCCATCC ACGTTGGCCA GGCTGGTGTC CAGATTGGCA ATGCCTGCTG GGAGCTCTAC 241 TGCCTGGAAC ACGGCATCCA GCCCGATGGC CAGATGCCAA GTGACAAGAC CATTGGGGGA 301 GGAGATGATT CCTTCAACAC CTTCTTCAGT GAGACGGGGG CTGGCAAGCA TGTGCCCCGG 361 GCAGTGTTTG TAGACTTGGA ACCCACAGTC ATTGATGAAG TTCGCACTGG CACCTACCGC 421 CAGCTCTTCC ACCCTGAGCA ACTTATCACA GGCAAGGAAG ATGCTGCCAA TAACTATGCC 481 CGAGGGCACT ACACCATTGG CAAGGAGATC ATTGACCTCG TGTTGGACCG AATTCGCAAG 541 CTGGCCGACC AGTGCACGGG TCTCCAGGGC TTCTTGGTTT TCCACAGCTT TGGTGGGGGA 601 ACTGGTTCTG GGTTCACCTC CCTGCTCATG GAACGTCTCT CAGTTGATTA TGGCAAGAAG 661 TCCAAGCTGG AGTTCTCTAT TTACCCGGCG CCCCAGGTTT CCACAGCTGT AGTTGAGCCC 721 TACAACTCCA TCCTCACCAC CCACACCACC CTGGAGCACT CTGATTGTGC CTTCATGGTA 781 GACAATGAGG CCATCTATGA CATCTGTCGT AGAAACCTCG ATATTGAGCG TCCAACCTAT 841 ACTAACCTGA ATAGGTTAAT AGGTCAAATT GTGTCCTCCA TCACTGCTTC CCTGAGATTT 901 GATGGAGCCC TGAATGTTGA CCTGACAGAA TTTCCAGACC AACCTGGTGC CCTATCCCCC 961 GCATCCACTT TCCCTCTGGG CCACATATGC CCCCTGTCAT CCTCCTGCCT GGAGAAAGGC 1021 CTACCCATGA ACAGGCTTTC CTGGTAAGCC AGAGATCACT ATGACGTTGC CTTTGAAGTC 1081 AAGGTGAACC AAGGAATTG // |
Primer: T7_20bp Sequence file: RDB14143_A6Dpb.seq |
>D03180A4_A6Dp_3_T7_20bp_H06_24.ab1 1 TCATTACTGG GTACTTATTA TTAAGGTACC GGGCCCTACT TATCGTCGTC ATCCTTGTAA 61 TCCTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT CCTGCAGGTA TTCCTCTCCT 121 TCTTCCTCAC CCTCTCCTTC AACAGAATCC ACACCAACCT CCTCATAATC CTTCTCAAGG 181 GCAGCCATGT CCTCACGGGC CTCTGAAAAC TCACCTTCCT CCATCCCCTC CCCAACGTAC 241 CAGTGAACAA AGGCACATTT GGCATACATC AGGTCAAACT TGTGGTCCAG GCGAGCCCAG 301 GCCTCAGCAA TGGCTGTGGT GTTGCTCAGC ATGCACACAG CTCTCTGTAC CTTGGCCAGG 361 TCTCCACCAG GCACCACAGT GGGAGGCTGG TAGTTGATGC CAACCTTGAA GCCAGTGGGG 421 CACCAATCCA CAAACTGGAT GGTACGCTTG GTCTTGATGG TGGCAATGGC AGCATTGACA 481 TCTTTGGGAA CCACGTCACC ACGGTACAAC AGGCAGCAAG CCATGTATTT ACCATGGCGA 541 GGGTCACATT TCACCATCTG GTTGGCTGGC TCAAAGCAAG CATTGGTGAT CTCTGCTACA 601 GAAAGCTGTT CATGGTAGGC TTTCTCAGCA GAGATGACAG GGGCATATGT GGCCAGAGGG 661 AAGTGGATGC GGGGATAGGG CACCAGGTTG GTCTGGAATT CTGTCAGGTC AACATTCAGG 721 GCTCCATCAA ATCTCAGGGA AGCAGTGATG GAGGACACAA TTTGACCTAT TAACCTATTC 781 AGGTTAGTAT AGGTTGGACG CTCAATATCG AGGTTTCTAC GACAGATGTC ATAGATGGCC 841 TCATTGTCTA CCATGAAGGC ACAATCAGAG TGCTCCAGGG TGGTGTGGGT GGTGAGGAAT 901 GGGAGTTTGT // |
Primer: SV40pro_F_V2 Sequence file: RDB14143_A6Dpc.seq |
>D03180A4_A6Dp_3_SV40pro_F_V2_D08_11.ab1 1 GTGCGGCTTT TTTGGGAGCC TAGGCTTTTG CAAGATCGAT CAAGAGACAG GATGAGGATC 61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG 121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG 181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA 241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC 301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC 361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA 421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA 481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT 541 GGACGAAGAA CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT 601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT 661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA 721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAA CTTGGCGGCG AATGGGCTGA 781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG 841 CCTTCTTGAC GAGTTCTTCT GAGCGGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC 901 GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC CTTCTATGAA AGGTTGGGCT 961 TCGGAATCGT TTTTCCGGGA CGCCCGGCTG GATGATCCCT CCAGCGGCGG GGATCTCATG 1021 CCTGGAAGTT TCATCGCCCA ACCCTAGGGG AGCCTACCTG AAACACGGAA GGAGACGATT 1081 ACCCGGA // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Yokoi, S., TUBA1A mutation can cause a hydranencephaly-like severe form of cortical dysgenesis. Sci. Rep. 5: 15165 (2015). PMID 26493046. [link to RRC of NBRP] |
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