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pCMV-TUBA1A flag-C25F (#RDB14142)

Expression vector of FLAG-tagged human TUBA1A mutant, C25F.

Clone info. Expression vector of FLAG-tagged human TUBA1A mutant, C25F.
Comment Expression was confirmed by the depositor with immunofluorescence.
Vector backbone pCMV-Tag 4 (Plasmid)
Size of vector backbone 4.3 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human tubulin alpha 1a cDNA
Depositor|Developer Kurahashi, Hiroki | Yokoi, Setsuri | Tsutsumi, Makiko |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yokoi, S. et al., Sci. Rep. 5:15165, 2015)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yokoi, S. et al., Sci. Rep. 5:15165, 2015)。

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RDB14142 pCMV-TUBA1A flag-C25F DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMV-TUBA1A flag-C25F was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14142).

Reference section:

Yokoi, S., Ishihara, N., Miya, F., Tsutsumi, M., Yanagihara, I., Fujita, N., Yamamoto, H., Kato, M., Okamoto, N., Tsunoda, T., Yamasaki, M., Kanemura, Y., Kosaki, K., Kojima, S., Saitoh, S., Kurahashi, H., Natsume, J., TUBA1A mutation can cause a hydranencephaly-like severe form of cortical dysgenesis. Sci. Rep. 5: 15165 (2015). PMID 26493046. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14142_A6Dpp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB14142_A6Dpa.seq check
>D03180A3_A6Dp_3_CMV-Forward_C06_09.ab1
    1 CGTTACTCAC CATTGACGCA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA
   61 GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCGATTACG CCAAGCTCGA AATTAACCCT
  121 CACTAAAGGG AACAAAAGCT GGAGCTCCAC CGCGGTGGCG GCCGCCATGC GTGAGTGCAT
  181 CTCCATCCAC GTTGGCCAGG CTGGTGTCCA GATTGGCAAT GCCTGCTGGG AGCTCTACTT
  241 CCTGGAACAC GGCATCCAGC CCGATGGCCA GATGCCAAGT GACAAGACCA TTGGGGGAGG
  301 AGATGATTCC TTCAACACCT TCTTCAGTGA GACGGGGGCT GGCAAGCATG TGCCCCGGGC
  361 AGTGTTTGTA GACTTGGAAC CCACAGTCAT TGATGAAGTT CGCACTGGCA CCTACCGCCA
  421 GCTCTTCCAC CCTGAGCAAC TTATCACAGG CAAGGAAGAT GCTGCCAATA ACTATGCCCG
  481 AGGGCACTAC ACCATTGGCA AGGAGATCAT TGACCTCGTG TTGGACCGAA TTCGCAAGCT
  541 GGCCGACCAG TGCACGGGTC TCCAGGGCTT CTTGGTTTTC CACAGCTTTG GTGGGGGAAC
  601 TGGTTCTGGG TTCACCTCCC TGCTCATGGA ACGTCTCTCA GTTGATTATG GCAAGAAGTC
  661 CAAGCTGGAG TTCTCTATTT ACCCGGCGCC CCAGGTTTCC ACAGCTGTAG TTGAGCCCTA
  721 CAACTCCATC CTCACCACCC ACACCACCCT GGAGCACTCT GATTGTGCCT TCATGGTAGA
  781 CAATGAGGCC ATCTATGACA TCTGTCGTAG AAACCTCGAT ATTGAGCGTC CAACCTATAC
  841 TAACCTGAAT AGGTTAATAG GTCAAATTGT GTCCTCCATC ACTGCTTCCC TGAGATTTGA
  901 TGGAGCCCTG AAATGTTGAC CTGACAGAAA TTCCAGACCA AACCTGGTGC CCTATCCCCC
  961 GCATCCCACT TCCCTCTGGC CACATATGCC CCCTGTCATC TCTGCTGGAG AAAGGCCTAC
 1021 CATGAACCAG GCTTTTCCTG GTAGCCAGAG ATCACGAATG CCATGCCATT GAAGCCAAGC
1081 CAACCAGAAA TTGGATTGA
//
Primer: T7_20bp
Sequence file: RDB14142_A6Dpb.seq check
>D03180A3_A6Dp_3_T7_20bp_G06_21.ab1
    1 TATTACTGGT ACTTATTATT AAGGTACCGG GCCCTACTTA TCGTCGTCAT CCTTGTAATC
   61 CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCC TGCAGGTATT CCTCTCCTTC
  121 TTCCTCACCC TCTCCTTCAA CAGAATCCAC ACCAACCTCC TCATAATCCT TCTCAAGGGC
  181 AGCCATGTCC TCACGGGCCT CTGAAAACTC ACCTTCCTCC ATCCCCTCCC CAACGTACCA
  241 GTGAACAAAG GCACGTTTGG CATACATCAG GTCAAACTTG TGGTCCAGGC GAGCCCAGGC
  301 CTCAGCAATG GCTGTGGTGT TGCTCAGCAT GCACACAGCT CTCTGTACCT TGGCCAGGTC
  361 TCCACCAGGC ACCACAGTGG GAGGCTGGTA GTTGATGCCA ACCTTGAAGC CAGTGGGGCA
  421 CCAATCCACA AACTGGATGG TACGCTTGGT CTTGATGGTG GCAATGGCAG CATTGACATC
  481 TTTGGGAACC ACGTCACCAC GGTACAACAG GCAGCAAGCC ATGTATTTAC CATGGCGAGG
  541 GTCACATTTC ACCATCTGGT TGGCTGGCTC AAAGCAAGCA TTGGTGATCT CTGCTACAGA
  601 AAGCTGTTCA TGGTAGGCTT TCTCAGCAGA GATGACAGGG GCATATGTGG CCAGAGGGAA
  661 GTGGATGCGG GGATAGGGCA CCAGGTTGGT CTGGAATTCT GTCAGGTCAA CATTCAGGGC
  721 TCCATCAAAT CTCAGGGAAG CAGTGATGGA GGACACAATT TGACCTATTA ACCTATTCAG
  781 GTTAGTATAG GTTGGACGCT CAATATCGAG GTTTCTACGA CAGATGTCAT AGATGGCCTC
  841 ATTGTCTACC ATGAAGGCAC AATCAGAGTG CTCCAGGGTG GTGTGGGTGG TGAAAGAAGT
901 AGATAGAATT TTGT
//
Primer: SV40pro_F_V2
Sequence file: RDB14142_A6Dpc.seq check
>D03180A3_A6Dp_3_SV40pro_F_V2_C08_08.ab1
    1 GTGGACTTTT TTTGGAGCTA GGCTTTTGCA AGATCGATCA AGAGACAGGA TGAGGATCGT
   61 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  121 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  181 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  241 AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  301 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  361 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  421 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  481 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  541 ACGAAGAACA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC
  601 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  661 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
  721 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAACT TGGCGGCGAA TGGGCTGACC
  781 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
  841 TTTCTTGACG AGTTCTTCTG AGCGGACTCT GGGGTTCGAA TGACCGACCA GCGACGCCCA
  901 ACCTGCATCA CGAGATTTCG ATTTCCACCG CCGCCTTCTA TGAAGTTGGG CTTCGGAATC
  961 GTTTTCGGAC GGCGCCTGGA ATGATTCTTC AGCGCGGGAA TTCTCATGGC TGGATTCTTT
1021 GGCCCACCTA GGAGCCTTAC TGACTACTCA G
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yokoi, S., TUBA1A mutation can cause a hydranencephaly-like severe form of cortical dysgenesis. Sci. Rep. 5: 15165 (2015). PMID 26493046. [link to RRC of NBRP]