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pCMV-TUBA1A flag-R64W (#RDB14141)

Expression vector of FLAG-tagged human TUBA1A mutant, R64W.

Clone info. Expression vector of FLAG-tagged human TUBA1A mutant, R64W.
Comment Expression was confirmed by the depositor with immunofluorescence.
Vector backbone pCMV-Tag 4 (Plasmid)
Size of vector backbone 4.3 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human tubulin alpha 1a cDNA
Depositor|Developer Kurahashi, Hiroki | Yokoi, Setsuri | Tsutsumi, Makiko |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yokoi, S. et al., Sci. Rep. 5:15165, 2015)
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提供依頼書  [依頼書の記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yokoi, S. et al., Sci. Rep. 5:15165, 2015)。

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RDB14141 pCMV-TUBA1A flag-R64W DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMV-TUBA1A flag-R64W was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14141).

Reference section:

Yokoi, S., Ishihara, N., Miya, F., Tsutsumi, M., Yanagihara, I., Fujita, N., Yamamoto, H., Kato, M., Okamoto, N., Tsunoda, T., Yamasaki, M., Kanemura, Y., Kosaki, K., Kojima, S., Saitoh, S., Kurahashi, H., Natsume, J., TUBA1A mutation can cause a hydranencephaly-like severe form of cortical dysgenesis. Sci. Rep. 5: 15165 (2015). PMID 26493046. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14141_A6Dpp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB14141_A6Dpa.seq check
>D03180A2_A6Dp_2_CMV-Forward_B05_05.ab1
    1 ATTTCACTCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGATTA CGCCAAGCTC GAAATTAACC
  121 CTCACTAAAG GGAACAAAAG CTGGAGCTCC ACCGCGGTGG CGGCCGCCAT GCGTGAGTGC
  181 ATCTCCATCC ACGTTGGCCA GGCTGGTGTC CAGATTGGCA ATGCCTGCTG GGAGCTCTAC
  241 TGCCTGGAAC ACGGCATCCA GCCCGATGGC CAGATGCCAA GTGACAAGAC CATTGGGGGA
  301 GGAGATGATT CCTTCAACAC CTTCTTCAGT GAGACGGGGG CTGGCAAGCA TGTGCCCTGG
  361 GCAGTGTTTG TAGACTTGGA ACCCACAGTC ATTGATGAAG TTCGCACTGG CACCTACCGC
  421 CAGCTCTTCC ACCCTGAGCA ACTTATCACA GGCAAGGAAG ATGCTGCCAA TAACTATGCC
  481 CGAGGGCACT ACACCATTGG CAAGGAGATC ATTGACCTCG TGTTGGACCG AATTCGCAAG
  541 CTGGCCGACC AGTGCACGGG TCTCCAGGGC TTCTTGGTTT TCCACAGCTT TGGTGGGGGA
  601 ACTGGTTCTG GGTTCACCTC CCTGCTCATG GAACGTCTCT CAGTTGATTA TGGCAAGAAG
  661 TCCAAGCTGG AGTTCTCTAT TTACCCGGCG CCCCAGGTTT CCACAGCTGT AGTTGAGCCC
  721 TACAACTCCA TCCTCACCAC CCACACCACC CTGGAGCACT CTGATTGTGC CTTCATGGTA
  781 GACAATGAGG CCATCTATGA CATCTGTCGT AGAAACCTCG ATATTGAGCG TCCAACCTAT
  841 ACTAACCTGA ATAGGTTAAT AGGTCAAATT GTGTCCTCCA TCACTGCTTC CCTGAGATTT
  901 GATGGGAGCC CTGAAATGTT GACCTGACAG AATTTCCAGA CCAAACCCTC TGCCCTATCC
  961 CCCGCATCCA CTTTCCCTCT GGGCCACATA TGCCCCCTGT CATCTCCTGC TGGAGAAAGG
 1021 CCTACCATGG AACCAGCTTT TCCTGGTAGC AGAAGATCAC GAATGCGTTG CATTGAAGTA
1081 GTCGAAGCCA AGAATTGGAT GA
//
Primer: T7_20bp
Sequence file: RDB14141_A6Dpb.seq check
>D03180A2_A6Dp_2_T7_20bp_F05_17.ab1
    1 TATTACTGGT ACTTATTAAT TAAGGTACCG GGCCCTACTT ATCGTCGTCA TCCTTGTAAT
   61 CCTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC CTGCAGGTAT TCCTCTCCTT
  121 CTTCCTCACC CTCTCCTTCA ACAGAATCCA CACCAACCTC CTCATAATCC TTCTCAAGGG
  181 CAGCCATGTC CTCACGGGCC TCTGAAAACT CACCTTCCTC CATCCCCTCC CCAACGTACC
  241 AGTGAACAAA GGCACGTTTG GCATACATCA GGTCAAACTT GTGGTCCAGG CGAGCCCAGG
  301 CCTCAGCAAT GGCTGTGGTG TTGCTCAGCA TGCACACAGC TCTCTGTACC TTGGCCAGGT
  361 CTCCACCAGG CACCACAGTG GGAGGCTGGT AGTTGATGCC AACCTTGAAG CCAGTGGGGC
  421 ACCAATCCAC AAACTGGATG GTACGCTTGG TCTTGATGGT GGCAATGGCA GCATTGACAT
  481 CTTTGGGAAC CACGTCACCA CGGTACAACA GGCAGCAAGC CATGTATTTA CCATGGCGAG
  541 GGTCACATTT CACCATCTGG TTGGCTGGCT CAAAGCAAGC ATTGGTGATC TCTGCTACAG
  601 AAAGCTGTTC ATGGTAGGCT TTCTCAGCAG AGATGACAGG GGCATATGTG GCCAGAGGGA
  661 AGTGGATGCG GGGATAGGGC ACCAGGTTGG TCTGGAATTC TGTCAGGTCA ACATTCAGGG
  721 CTCCATCAAA TCTCAGGGAA GCAGTGATGG AGGACACAAT TTGACCTATT AACCTATTCA
  781 GGTTAGTATA GGTTGGACGC TCAATATCGA GGTTTCTACG ACAGATGTCA TAGATGGCCT
  841 CATTGTCTAC CATGAAAGGC ACAATCAGAG TGCTCCAGGG TGGTGTGGGT GGGTGAAGGA
901 ATGGAAGTT
//
Primer: SV40pro_F_V2
Sequence file: RDB14141_A6Dpc.seq check
>D03180A2_A6Dp_2_SV40pro_F_V2_F07_16.ab1
    1 GTGGGCTTTT GGAGGCTAGG CTTTTGCAAG ATCGATCAAG AGACAGGATG AGGATCGTTT
   61 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
  121 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
  181 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
  241 CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
  301 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
  361 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
  421 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
  481 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
  541 GAAGAACATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC
  601 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA
  661 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG
  721 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAACTTG GCGGCGAATG GGCTGACCGC
  781 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT
  841 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGGTTCGAAA TGACCGACCA AGCGACGCCC
  901 CAACCTGCCA TCACGAGATT TCGATTCCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG
  961 GAATCGTTTT TCCCGGGACG CCGGCTGAAT GATCCCTCCC AGCGGCGGGG ATCTCATGGC
 1021 TGCAGTTCTT CGCCAACCCT TAAGGGGAGG CTACTGCAAA CACGGAGGAG AACATACGCG
1081 GCAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yokoi, S., TUBA1A mutation can cause a hydranencephaly-like severe form of cortical dysgenesis. Sci. Rep. 5: 15165 (2015). PMID 26493046. [link to RRC of NBRP]