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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMV-TUBA1A flag-WT (#RDB14140)

Expression vector of FLAG-tagged human TUBA1A (wild type).

Clone info. Expression vector of FLAG-tagged human TUBA1A (wild type).
Comment Expression was confirmed by the depositor with immunofluorescence.
Vector backbone pCMV-Tag 4 (Plasmid)
Size of vector backbone 4.3 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human tubulin alpha 1a cDNA
Depositor|Developer Kurahashi, Hiroki | Yokoi, Setsuri | Tsutsumi, Makiko |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yokoi, S. et al., Sci. Rep. 5:15165, 2015)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yokoi, S. et al., Sci. Rep. 5:15165, 2015)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14140 pCMV-TUBA1A flag-WT DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMV-TUBA1A flag-WT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14140).

Reference section:

Yokoi, S., Ishihara, N., Miya, F., Tsutsumi, M., Yanagihara, I., Fujita, N., Yamamoto, H., Kato, M., Okamoto, N., Tsunoda, T., Yamasaki, M., Kanemura, Y., Kosaki, K., Kojima, S., Saitoh, S., Kurahashi, H., Natsume, J., TUBA1A mutation can cause a hydranencephaly-like severe form of cortical dysgenesis. Sci. Rep. 5: 15165 (2015). PMID 26493046. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14140_A6Dpp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB14140_A6Dpa.seq check
>D03180A1_A6Dp_1_CMV-Forward_A04_01.ab1
    1 ATTTACTCGA CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCGATTAC GCCAAGCTCG AAATTAACCC
  121 TCACTAAAGG GAACAAAAGC TGGAGCTCCA CCGCGGTGGC GGCCGCCATG CGTGAGTGCA
  181 TCTCCATCCA CGTTGGCCAG GCTGGTGTCC AGATTGGCAA TGCCTGCTGG GAGCTCTACT
  241 GCCTGGAACA CGGCATCCAG CCCGATGGCC AGATGCCAAG TGACAAGACC ATTGGGGGAG
  301 GAGATGATTC CTTCAACACC TTCTTCAGTG AGACGGGGGC TGGCAAGCAT GTGCCCCGGG
  361 CAGTGTTTGT AGACTTGGAA CCCACAGTCA TTGATGAAGT TCGCACTGGC ACCTACCGCC
  421 AGCTCTTCCA CCCTGAGCAA CTTATCACAG GCAAGGAAGA TGCTGCCAAT AACTATGCCC
  481 GAGGGCACTA CACCATTGGC AAGGAGATCA TTGACCTCGT GTTGGACCGA ATTCGCAAGC
  541 TGGCCGACCA GTGCACGGGT CTCCAGGGCT TCTTGGTTTT CCACAGCTTT GGTGGGGGAA
  601 CTGGTTCTGG GTTCACCTCC CTGCTCATGG AACGTCTCTC AGTTGATTAT GGCAAGAAGT
  661 CCAAGCTGGA GTTCTCTATT TACCCGGCGC CCCAGGTTTC CACAGCTGTA GTTGAGCCCT
  721 ACAACTCCAT CCTCACCACC CACACCACCC TGGAGCACTC TGATTGTGCC TTCATGGTAG
  781 ACAATGAGGC CATCTATGAC ATCTGTCGTA GAAACCTCGA TATTGAGCGT CCAACCTATA
  841 CTAACCTGAA TAGGTTAATA GGTCAAATTG TGTCCTCCAT CACTGCTTCC CTGAGATTTG
  901 ATGGAGCCCT GAATGTTGAC CTGACAGAAT TTCCAGACCA ACCTGGTGCC CTATCCCCGC
  961 ATCCCACTTT CCCTCTGCCC ACATATGCCC CCTGTCATCC TCCTGCCTGG AGAAAGGCCT
 1021 ACCCATGGAA CAGGCTTTCT GGTAGGCAGA GGATTCACGA TGCTGGCCTT TGAGCAAGCC
1081 GAACCCAGAA ATTGCT
//
Primer: T7_20bp
Sequence file: RDB14140_A6Dpb.seq check
>D03180A1_A6Dp_1_T7_20bp_E04_13.ab1
    1 TCATTTACAG CTACTTATTA TTAAGGTACC GGGCCCTACT TATCGTCGTC ATCCTTGTAA
   61 TCCTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT CCTGCAGGTA TTCCTCTCCT
  121 TCTTCCTCAC CCTCTCCTTC AACAGAATCC ACACCAACCT CCTCATAATC CTTCTCAAGG
  181 GCAGCCATGT CCTCACGGGC CTCTGAAAAC TCACCTTCCT CCATCCCCTC CCCAACGTAC
  241 CAGTGAACAA AGGCACGTTT GGCATACATC AGGTCAAACT TGTGGTCCAG GCGAGCCCAG
  301 GCCTCAGCAA TGGCTGTGGT GTTGCTCAGC ATGCACACAG CTCTCTGTAC CTTGGCCAGG
  361 TCTCCACCAG GCACCACAGT GGGAGGCTGG TAGTTGATGC CAACCTTGAA GCCAGTGGGG
  421 CACCAATCCA CAAACTGGAT GGTACGCTTG GTCTTGATGG TGGCAATGGC AGCATTGACA
  481 TCTTTGGGAA CCACGTCACC ACGGTACAAC AGGCAGCAAG CCATGTATTT ACCATGGCGA
  541 GGGTCACATT TCACCATCTG GTTGGCTGGC TCAAAGCAAG CATTGGTGAT CTCTGCTACA
  601 GAAAGCTGTT CATGGTAGGC TTTCTCAGCA GAGATGACAG GGGCATATGT GGCCAGAGGG
  661 AAGTGGATGC GGGGATAGGG CACCAGGTTG GTCTGGAATT CTGTCAGGTC AACATTCAGG
  721 GCTCCATCAA ATCTCAGGGA AGCAGTGATG GAGGACACAA TTTGACCTAT TAACCTATTC
  781 AGGTTAGTAT AGGTTGGACG CTCAATATCG AGGTTTCTAC GACAGATGTC ATAGATGGCC
  841 TCATTGTCTA CCATGAAGGC ACAATCAGAG TGCTCCAGGG TGGTGTGGGT GGGGTGGGGG
901 AGGTGTGGGA GTTG
//
Primer: SV40pro_F_V2
Sequence file: RDB14140_A6Dpc.seq check
>D03180A1_A6Dp_1_SV40pro_F_V2_H06_24.ab1
    1 GGTCCCACTT CTTTTGGGAG GCCTTAGGCC TTTTGCAAAG ATCGATCAAA GAGACAGGAT
   61 GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG
  121 TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG
  181 TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG
  241 CCCTGAATGA ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC
  301 CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG
  361 AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA
  421 TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC
  481 AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG
  541 ATGATCTGGA CGAAGAACAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG
  601 CGAGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA
  661 TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG
  721 ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAACTT GGCGGGCGAA
  781 TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCCGATTCGC AGCGCATCGC
  841 CTTTCTATCG CCTTCTTGAC GAGGTTTCTT TCTGAGCGGG GACTCTGGGG GGTTCGAAAA
  901 TGAACCGAAC CAAGCGAACG CCCCAACCCT GCCAATCACG AGATTTCGAT TTCCCACCGG
  961 CCCGCCTTTC TAATGAAAGG GTTGGGGCTT TCGGAAATCG TTTTTTCCGG GACGTCCGGG
 1021 CCTGGGATTG AATCCCTTCC CAGCGGCGGG GGAATCTCAT GGCTGGGGAG TTTCCTTCGC
 1081 CCACCCCTAA GGGGGGAGGG CTTAAGCTTG CAAAACTACT CGGGAATGGG AGAGATACCA
1141 ATGTAAACGA CG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yokoi, S., TUBA1A mutation can cause a hydranencephaly-like severe form of cortical dysgenesis. Sci. Rep. 5: 15165 (2015). PMID 26493046. [link to RRC of NBRP]