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pDll1-Ub-Fluc

Reporter vector for time-lapse imaging of Dll1 transcription.

Catalog number RDB14127
Resource name pDll1-Ub-Fluc
Clone info. Reporter vector for time-lapse imaging of Dll1 transcription. This construct contains Dll1 promoter and destabilized luciferase cDNA.
Expression was confirmed by the depositor with bioluminescence imaging. See Fig.1 of Shimojo, H. et al., Genes Dev. 30 (1): 102-116, 2016.
Vector backbone pBluescript (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Mouse Dll1 genomic DNA firefly (Photinus pyralis) cDNA
Depositor Kageyama, Ryuichiro |
 
Other clones in our bank mouse Dll1 (NCBI Gene 13388) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Shimojo, H. et al., Genes Dev. 30(1):102-116, 2016)
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RDB14127 pDll1-Ub-Fluc DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Notch signaling pathway (English text)

References

original Shimojo, H., Oscillatory control of Delta-like1 in cell interactions regulates dynamic gene expression and tissue morphogenesis. Genes Dev. 30 (1): 102-116 (2016). PMID 26728556.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14127_A6Djp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 RDB14127_A6Dja.seq
>D03343A1_A6Dj_2_M13_-40_E03_15.ab1
    1 CCTGCACAGG GATTGTATAC GACTCACTAT AGGGCGAATT GGGTACCGGG CCCCCCCTCG
   61 AGGTCGACGC GGCCGCTTGT AGTTCATTAT ATTTATTTTG GAAAAATATT TTTAAAATGA
  121 ATTTCTTCAT TAACAAAACA GTAAAAAACT CAAATAACAT TTGCACAATA TTCCATAAAT
  181 ACATTTATAT ACAAAAAAAT ATAGTCACAT AGACCCGAAG TGCCTTTGTA CATATTTACC
  241 AAAATTTAAA TTATAAAAAA ATGAACGTCA CAAAATACTC AAGCTTTACA GATATCATGA
  301 AAAATATTTT TACAAATCCC AAAAAAATAA CACACGTTTT CTTTTTCCCG TCTAGGAAAA
  361 AAAAAAACAC ATCTCAGTAT CAAAAAGGAC AATAAAGGCA GTTGTGTTTC TAGTTCAAGG
  421 AAAGACTGGC TCATAGTAAT CCAAGATAGA CGTGTGGGCA GTGCGTGCTT CCTATATGAA
  481 TCAAGTCACT CGTCCATTTA AATATATACT CTTCAAAGCA ACTGTCCATA GTGCAATGGG
  541 AACAACCAGC AGGCAGTCCA GCGGCAGCCA AAGCCTAGGC TGGCAGTGTC GGGCGCCTCT
  601 GCTAACTCTG AGAGAACCAG CTTCGCCTGA ACCTGGTTCT CAGCAGCAGT CCCTGGGTTT
  661 CCTCTCCCTT CCTCTCTCAG CAGCATTCAT CGGGGCTATA TCCTTGGAAT TTTATTTAAG
  721 AGAAATGGGA ATTTTGCCAC ATCGCTTCCA TCTTACACCT AGAAGGGGTC GACGGTATCG
  781 ATGTGAGGTT ACACGGCGAT CTTTCCGCCC TTCTTGGCCT TTATGAGGAT CTCTCTGATT
  841 TTTCTTGCGT CGAGTTTTCC GGTAAGACCT TTCGGTACTT CGTCCACAAA CACAACTCCT
  901 CCGCGCAACT TTTTTCGCGG TTGTTACTTT GACTGGCGAC GTAATCCACG ATCTCTTTTT
  961 TCCGTCATCG TCTTTCCGTG CTCCCAAAAC AACAACGGCG GCGGGAAAGT TTCACCGGCG
 1021 TCATCGTCGG AAGACTGCGA CACCTGCGTC GAAGATGTGG GGTGGTTGGA GCAGGATTGA
1081 TCCGAATCAG CGGGAAGCCA CCTTGATAGC CTGTTTTGTT ACCTA
//
Primer: Reverse2 RDB14127_A6Djb.seq
>D03343A1_A6Dj_2_Reverse2_H10_22.ab1
    1 ATACCGGGGA ATTACGGCCC AAAGCTCGAA ATTAACCCTC ACTAAAGGGA ACAAAAGCTG
   61 GAGCTCCACC GCGGTGGCGG CCGCTCTAGA ACTAGTCCTG ATACCAGGTG AGGTGAGAGC
  121 ACTGTGGCCC GGGCCCAGCT GCATGGAACC AGCACGACTT CGTCTCAAAG CTTTGTGACT
  181 CAAGTCGTAC CCACATCCAC AGGCCCAGCG ATGGCCACCT CTCTAGCACC GCCACCGTCC
  241 AGAAGCCCCC AGGGGCGCTA ACTCTAGTTG AGAGTCAGAA ACGTATGAGC AAACTAAACG
  301 CAACCCCTCC CCTGTTAGAG CCTTCTTCCC ACAAACCCCT CACCCCATTG CTACCCATTC
  361 CAGACCAAAG GCCGCGGAAA GTTCCGAGCG AGGTGCACGC GTGACTCTCT TCGGCTGGCC
  421 ACGAGCAGCT GCTAGGATAA TCTGATCCTG GGACAAAGGC CCGCCCGCCC GGGCTGCAGC
  481 CGCAGGTAAA CACGCGCTTT GTTATGGAAA TCAGCGCAGG ACAGCCCGGA GCAGACAACC
  541 CACGATTCCG AGGAAGGAGA GGCATAGGGG GAGGGGCCTC GGGTGGGGCG ATCTGTCATC
  601 TGCGCGGCAG CCGCCAGCTG AGCCACGCGC CTCCCCTTTT CTCATGTAAA TCAGCACATG
  661 CCTTTGCAAC TTTCTGCAGC CGGCTTCCCA AGACCCACGC ACCCCAGGGT GAGGTGTCCT
  721 CGGAGCGCAT ACAATGGCTC TGAGTTCCTA TCTAGCTACC CAAAGGAAAA TCACCAACTA
  781 GGTGGGTAGA AGGAGGCGGC AGCCGGTTCC ATCAAAGAAG AAAGGGCTGT GATCCTACGG
  841 CATGACGGAG TTCGGACCCC GATCCACTCT GAAGTTCACG GGGCGCCATG CCGGAGTCTT
  901 TTGGGTTCAT TACTCCTTTT TGAGTGTACT GCCCACAGAA CGCAGAGGAG GCTAGGCCTA
  961 CCGGTTCCAC CTGGTGGGTG TGAATCTGAA GGCTCGAAGT CTCTAGTCTA ATTGGAAAGC
 1021 CAAATGCCTG AATTGCATTC AAGTGTGCAC ATGGTCTGTA CAACCATTAA GTGTGTGCGC
1081 CAAGTATAAT GGGTTGGCAT GGCCTTTGTA A
//
Primer: Luc_R RDB14127_A6Djc.seq
>D03343A1_A6Dj_2_Luc_R_B01_04.ab1
    1 CATATGCTTC AAGGAACAGA GCGTATCTCT TCATAGCCTT ATGCAGTTGC TCTCCAGCGG
   61 TTCCATCTTC CAGCGGATAG AATGGCGCCG GGCCTTTCTT TATGTTTTTG GCGTCTTCAA
  121 GCTTACTAGT CACCACACCT CTGAGACGGA GTACCAGGTG CAGGGTGGAC TCTTTCTGGA
  181 TGTTGTAGTC AGACAGGGTG CGCCCATCTT CCAGCTGTTT TCCAGCAAAG ATCAGCCTCT
  241 GCTGGTCAGG AGGGATGCCT TCCTTATCTT GGATCTTTGC CTTGACATTC TCAATGGTGT
  301 CACTGGGCTC AACCTCGAGG GTGATGGTCT TACCAGTCAG AGTCTTCACG AAGATCTGCA
  361 TGGTGGCGGC GGTTACCTTA ACATCGATAA GCTTGATATC GAATTCCTGC AGCCCGGGGG
  421 ATCCACTAGT CCGCTGGACG CCCTTGCTCT CCTAGAGTAT CCTCTTTTCT TTCTTCTTCA
  481 AAGACCCAGG GATGCCAGGA AAGTCTATAT GACCCGAGGA GTACTGGCGG AAAGGCGTCT
  541 CTCGGCTCCA GGTGTGCTAT CAGGGCCAGG TCGCTTCAGG AGGGTGCGGT GCCTTCAGCT
  601 AGGTCCCCGG CAAGAGCTCT ACGAGGCACT GAGAAAGGAC AACTTTCGGT TTCCTCTTCG
  661 TCTGGCTTTC AGTCCACGGA GAGGTGAGTG TCTCCTGCTT TCTGGTTTTG TCTTAAGCTT
  721 CTTCGCGGAG GGAAAGAGGA GAAAGGAGAT AAGACACAGA GGGAAGAGAG CCCAGATGTT
  781 CAGCTTAATT CCCAAGAGAG CTTGCAGTGG CTTCCTTCGC AGGCCTATTA GGAAGCGGCG
  841 GTCTGGAAAT CCCACCGGTG AGGCGATAAT CCGGGGCCCC GAGGGGCGCG GTGGGAACGG
  901 TGGTGGCAAG GACTGCGGCG CGCGCCCTGG TTTCCCGGCC CGGCGTCACT CGGCAGCCCG
  961 CTGCCGCTCC CAGAGTGTTC TAGACCGGGT ATCTCTTGCA CTATCGCCAG CCTCGCGTCT
 1021 TTCCCTCAAG AATTTCAGCC CAATGCCCAT GCGAGACACA CACCGAAGAA CCGGAGCCCG
1081 AAAGCGTGGG AAGCTCGTTG CCGCACCGGG TGCTTCTTCC CCGGTGTAAT CT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Shimojo, H., Oscillatory control of Delta-like1 in cell interactions regulates dynamic gene expression and tissue morphogenesis. Genes Dev. 30 (1): 102-116 (2016). PMID 26728556.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl