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pCAGIPuro-HAmJarid2WT (#RDB14117)

Expression vector of HA tagged mouse Jarid2, wild type.

Alternative name clone 14
Clone info. Expression vector of HA tagged mouse Jarid2, wild type.
Comment Expression was confirmed by the depositor. SV40 ori, IRES-puromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20.
Vector backbone pCAG-IP (Plasmid)
Size of vector backbone 7.2 kb
Selectable markers Amp^r (E. coli), Pur^r (mammalian cells).
Gene/insert name mouse Jarid2 cDNA
Depositor|Developer Okazaki, Yasuji | Iseki, Hiroyoshi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)
Additional terms and conditions:
CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)。
MTAに書く付加的使用条件：
利用者は、pCAG vector に挿入されている遺伝子群を使用・公表する場合は、下記文章の掲載を必要とする ( CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J. )。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14117 pCAGIPuro-HAmJarid2WT DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAGIPuro-HAmJarid2WT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14117).

Reference section:

Iseki, H., Nakachi, Y., Hishida, T., Yamashita-Sugahara, Y., Hirasaki, M., Ueda, A., Tanimoto, Y., Iijima, S., Sugiyama, F., Yagami, K., Takahashi, S., Okuda, A., Okazaki, Y., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14117_A6Dcp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB14117_A6Dca.seq check
>D03255K9_A6Dc_pAxCA_F1_B03_06_ABI24.ab1
    1 GTGAGGGGGT CCTGCTACAT GTTCATGCCT TCTTCTTTTT CCTACAGCTC CTGGGCAACG
   61 TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT CCTCGACCAC CATGTACCCT
  121 TACGACGTGC CTGACTACGC CGGAGGAGGC ATGAGCAAGG AAAGACCCAA GAGGAATATC
  181 ATTCAGAAGA AATACGATGA CAGCGATGGG ATCCCGTGGT CAGAAGAGAG AGTTGTACGA
  241 AAAGTCCTGT ATTTGTCCCT AAAGGAATTC AAGAATGCAC AGAAAAGGCA GCATGGGGAA
  301 GGCCTTGCCG GGAGCCTGAA GGCGGTAAAT GGGCTTCTTG GTAATGCCCA GGCTAAGGCA
  361 CTAGGACCAG CCTCAGAGCA GTCAGAGAAC GAGAAGGATG ATGCCTCCCA AGTGTCCTCT
  421 ACTAGCAACG ATGTTAGTTC TTCAGATTTT GAAGAAGGGC CGTCGAGGAA AAGGCCCAGG
  481 CTGCAAGCAC AAAGGAAGTT TGCTCAATCT CAGCCGAATA GTCCCAGCAC AACTCCAGTG
  541 AAGATAGTGG AGCCTTTGCT ACCCCCGCCA GCTACTCAAA TTTCTGACCT CTCTAAAAGG
  601 AAGCCTAAGA CAGAAGACTT TCTTACCTTT CTCTGCCTTC GAGGTTCTCC TGCGCTGCCC
  661 AACAGTATGG TATATTTTGG AAGCTCTCAG GATGAGGAGG ATGTCGAAGA GGAAGATGAT
  721 GAGACGGAAG ATGTCAAAGC AACCACCAAC AATGCTTCAT CTTCGTGCCA GTCAACCCCC
  781 AGGAAAGGAA AAACCCATAA GCATGTACAC AACGGGCACG TTTTCAATGG CTCCAGTATG
  841 TCAGCACGGG AGAAAGAGCC TGCTCACAAA CACAGAAGCA AAGAGGCCAC TCCGGGGAAA
  901 GGAGAAGCAC AGCGAGCCCA GGCAGACAGC CGGGAGAGAA CAGGCTTCAG GGGCTCAGCC
  961 CACGGCCGCC TCGGCTGCGG CTTTCCTCTG CCAAGGGACT TGCTGCCACA CCAACCTCCC
 1021 CCTTTCGCAT CGGTCTGCTC AAGACTTTAC GAACCAGTGT CTAGTAAATT GTGTCACTCG
1081 TATGTCATCT CTTGGTGCAA GTACCAAAAC AAG
//
Primer: IRES-R
Sequence file: RDB14117_A6Dcb.seq check
>D03255K9_A6Dc_IRES-R_C03_09_ABI24.ab1
    1 GGACATGCAG CGTGCGACAG TGCGTGAGGC GGGGGGGAGG GAGAGGGGGG GGGGGCGGAA
   61 TTCGATATCA AGCTTATCGA GCGGCCGCTC GATACCGTCG ATCTCAGTGG TATTTGTGAG
  121 CCAGTTGGCC ACACCAGCCA CCACCTTCTG ATAGGCAGCC TGCACCTGAG GAGTGAATTC
  181 CTCGACTCAT GAGGATGGGA GCCGAGATGG CGGCACATCC ACGGTCGCTC TCTTGCGGGG
  241 ACCTCTTTTT GGTGTGGGTT TGTTGAGACA GTTCTCGATG CCACCGTGTT TGCCAGACAC
  301 TTTGCCACAT ATCTGGTTGA CCAAACTGAT GATCTGCTCC TCATCGTAGC GGTACATCAG
  361 CTTCAGCCCA CGGCAAGACT TCTGCTTCTC CACATGGCGC AGTGCACACT CCAGGCAGAA
  421 CACGACATTC TCATTCTCCT GTACCACCAT GGACAGGTAG CACAAGTGCT GGCAGATCTG
  481 GCACCTCCTT TCTGAAGTTT CCAGCTGTAG CCACTTTCGA GGCTTTTTCT TCCCATCCGC
  541 CACTGTGCTG TTGCCGTCGT GGCTGCCATA GCGTGCAGAA GAGTGAAGGC CAGCCTCGAA
  601 CAACAGCCTG CGCTGCCGCA GCTCTGTATC CCTGAGCTCA TCCAGAAGGG CTGAGATGGT
  661 ACTGAGGGTG GGACCATTTT CCTTTTTTGC TTCTGCTTGT GCAATCTGGT AGAGCAACTT
  721 CTCCATGGAG AATGGCTTAG CTATATGGCG GCGCTTCATC TCCTTGGCAG TCTCGAAGCC
  781 CATGCTTGTC CACTGGGTGG TAGCAAAGTG CACTGTCTCT GAGACGTTGT AGCCACAACA
  841 TACTTTGGAC ACAAAGGATC CCGGGAAGCA GACGACAAAC TGCCCACTCT GCTGTACAGT
  901 CCTGTGGACC TTGATCCCCT TTCTTGCACA GCACCTCTGG GGAGATCATT ACATTGCTCT
  961 CCAGCATCTG CAGCCCTGGG GTACCATTGC CCTGCAGCAG AGTGTGACTA CGTCCTCCAG
 1021 CCTGGTCTCT CTCAGCAAGA TGCATTACGA ATTGCAGTCA GCACCATGTG TAAGTAGTCA
1081 TATATGGCAA GTGAATTTTG GATCCTCCGG GAG
//
Primer: IRES-F
Sequence file: RDB14117_A6Dcc.seq check
>D03255K9_A6Dc_IRES-F_D03_12_ABI24.ab1
    1 GGCAGTCGTT AATGTGTTTA GTCGAGGTTA AAAAACGTCT AGGCCCCCCG AACCACGGGG
   61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGAC CGAGTACAAG
  121 CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCAGGG CCGTACGCAC CCTCGCCGCC
  181 GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGATC CGGACCGCCA CATCGAGCGG
  241 GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG
  301 GTCGCGGACG ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG
  361 GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG
  421 CAGCAACAGA TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC GTGGTTCCTG
  481 GCCACCGTCG GCGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC
  541 CCCGGAGTGG AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC
  601 CGCAACCTCC CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT CGAGGTGCCC
  661 GAAGGACCGC GCACCTGGTG CATGACCCGC AAGCCCGGTG CCTGACGCCC GCCCCACGAC
  721 CCGCAGCGCC CGACCGAAAG GAGCGCACGA CCCCATGCAT CGATGATCTA GAGCTCGCTG
  781 ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC
  841 TTCCTTGACC CTGGAAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG
  901 CATCGCATTG TCTGAGTAGT GTCATTCTAT TCTGGGGGGG TGGGGTGGGG CAGACAGCAA
  961 GGGGGAGGAT TGGGAAGACA TAGCAGGCAT GCTGGGGGAT GCGGTGGGCT CTATGGCTTT
 1021 CTGAGCCGAA AGAACTGCAG CCCAGCCTGG CCGTATTCAT GGTCATAGCC TGATTCCTGT
1081 GGTGAATGGT AATCCGGCCT CAACAATTTC ACCA
//
Primer: BGHpA_F
Sequence file: RDB14117_A6Dcd.seq check
>D03255K9_A6Dc_BGHpA_F_E10_13_ABI24.ab1
    1 TTGTAATGTT ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG
   61 GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA
  121 ACCTGCAGCC CAAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT
  181 CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC
  241 TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA
  301 AACCTGTCGT GCCAGCGGAT CCGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA
  361 CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC
  421 TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT
  481 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTAACTTGT TTATTGCAGC
  541 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
  601 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCCG
  661 CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC
  721 GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT
  781 CACTCAAAGG CGGTAATACG GTTATCCACA GAATCACGGG ATAACGCAGA AAGAACATGT
  841 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC
  901 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA
  961 ACCCGACAGG ACTATAAGAT ACCAAGGCGT TCCCCCCTGG AGCTCCCTCG TGCGCTCCTC
 1021 TGATCGACCC TGCGCTTACT GCATACTGTC GCATTCTCTT CGGTAAGCGT GCGCATTCTC
1081 ATAGGCTCAC AGC
//
Primer: Amp_R
Sequence file: RDB14117_A6Dce.seq check
>D03255K9_A6Dc_Amp_R_F10_16_ABI24.ab1
    1 CTCGTTTTCA TGACCGCTTT GGAAACAAAG ACTGTATTTC CTGGAAATTA ATGTTTATTC
   61 AATAAACTGT GTATTCAGCT ATATTCACAT AGTGGTGAGG CTGAAATGAG GCGGGAAGAG
  121 GCGGTTGGGG CTTAATTATA TCAATTTGGG TGGCCCCACA GCGCCTCCAA GGCGCCAGTC
  181 CTGTTTTGAC AAGTTGCCTC TGGAAGCCTC TCTTCTTTTT CTCCAGAGTA AGCGGAGGCC
  241 AGGGGCCCCC GGCCTCTGCT TAATACTAAA AAAAACAGCT GTTGTCATAG TAATGATTGG
  301 GTGGAAACAT TCCAGGCCTG GGTGGAGAGG CTTTTTGCTT CCTCTTGCAA AACCACACTG
  361 ACATTCCAGG CCTGGGTGGA GAGGCTTTTT GCTTCCTCTT GCAAAACCAC ACTGCCCTCT
  421 GGAGGGCAGT TGCCTAGCAA CTAATTAAAA GAGGATGTCG CACGGCCAGC TGCGGTCAGT
  481 TAGTCACTTC CTGCTTAACT GACTTGACAT TTTCTATTTT AAGAGTCGGG AGGAAAATTA
  541 CTGTGTTGGA GGCCCTCCGC CATCTTCTGA AGCTGAATCG AATTAACTTG TTTATTGCAG
  601 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT
  661 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGATCT
  721 GATATCATCG TCGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT
  781 TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG
  841 GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT TCCCATAGTA
  901 ACGCCAATAG GGACTTTCCA TTGACGTCAT GGGGTGGAGT ATTTACGGTA ACTGCCCACT
  961 GCAGTACATC AGTGTATCAT ATGCAGTACG CCCCCTATTG ACGTCCATGA CCGTAATTGA
1021 CCGCGTGGCA TTATGGCCAG TACATGACGT ATGACGTTCC TACTGCAGTA CATCTTACG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Iseki, H., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946. [link to RRC of NBRP]